Psychroflexus torquis (strain ATCC 700755 / ACAM 623)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Psychroflexus; Psychroflexus torquis

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3457 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4ID11|K4ID11_PSYTT Uncharacterized protein OS=Psychroflexus torquis (strain ATCC 700755 / ACAM 623) OX=313595 GN=P700755_001382 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.47NILLLAFTFLFFSSQILSAQSQNKK26 pKa = 9.57HH27 pKa = 5.61IEE29 pKa = 4.19TFLSSKK35 pKa = 10.42VSSKK39 pKa = 10.91KK40 pKa = 10.05LAQNDD45 pKa = 2.74ISEE48 pKa = 4.38YY49 pKa = 10.79VITSQHH55 pKa = 6.27KK56 pKa = 9.78SSISNIEE63 pKa = 3.88HH64 pKa = 6.25IYY66 pKa = 10.17FRR68 pKa = 11.84QSIDD72 pKa = 3.04GVSIVGTEE80 pKa = 4.12SSIHH84 pKa = 5.83VYY86 pKa = 10.22PDD88 pKa = 3.12EE89 pKa = 4.33TLLRR93 pKa = 11.84ANFGFKK99 pKa = 10.36KK100 pKa = 10.54SIADD104 pKa = 3.58KK105 pKa = 11.09VIGAKK110 pKa = 8.24TPKK113 pKa = 10.28LSAEE117 pKa = 3.78LAIRR121 pKa = 11.84RR122 pKa = 11.84VAQQMGYY129 pKa = 8.5YY130 pKa = 9.75QSGGLNSLPKK140 pKa = 9.72STIPNSDD147 pKa = 3.64YY148 pKa = 11.0VFSGAGISEE157 pKa = 4.03VNIPVKK163 pKa = 10.31LVYY166 pKa = 10.61VKK168 pKa = 10.84LEE170 pKa = 3.62QSKK173 pKa = 9.79FVLAWEE179 pKa = 4.47LSIKK183 pKa = 7.19EE184 pKa = 4.52TKK186 pKa = 10.57SSDD189 pKa = 2.76WYY191 pKa = 11.27NFYY194 pKa = 11.52ADD196 pKa = 4.02ASTGKK201 pKa = 10.22IISKK205 pKa = 10.43NNWTVNCLEE214 pKa = 4.17GHH216 pKa = 6.41DD217 pKa = 4.16HH218 pKa = 5.67STHH221 pKa = 6.47NEE223 pKa = 3.9SEE225 pKa = 4.2KK226 pKa = 10.66PKK228 pKa = 10.91AFVSVKK234 pKa = 10.57NKK236 pKa = 7.82PTTTNSGLSQGTYY249 pKa = 10.19NVFPLPVEE257 pKa = 5.12DD258 pKa = 4.67PFSDD262 pKa = 3.42PRR264 pKa = 11.84EE265 pKa = 3.95LVSNSNNALASPFGWHH281 pKa = 6.7DD282 pKa = 3.7TNGSIGAEE290 pKa = 3.85FTVTRR295 pKa = 11.84GNNVNAYY302 pKa = 10.0EE303 pKa = 4.68DD304 pKa = 4.13GNNQGFQPDD313 pKa = 4.09GGSSLSFDD321 pKa = 4.04FPFNPDD327 pKa = 3.1YY328 pKa = 11.59SFGDD332 pKa = 3.53QSEE335 pKa = 4.53SAAITNLFYY344 pKa = 10.68WNNIIHH350 pKa = 7.3DD351 pKa = 4.59IIYY354 pKa = 10.26QYY356 pKa = 11.54GFDD359 pKa = 3.62EE360 pKa = 4.81ASGNFQVNNYY370 pKa = 8.77GNPGVGNDD378 pKa = 3.83PVLAEE383 pKa = 4.31AQDD386 pKa = 3.87GGGTCNANFSRR397 pKa = 11.84APEE400 pKa = 4.76GISPVMQMYY409 pKa = 9.97ICNNRR414 pKa = 11.84DD415 pKa = 2.77GDD417 pKa = 3.79FDD419 pKa = 4.17NGVIIHH425 pKa = 6.98EE426 pKa = 4.4YY427 pKa = 9.58GHH429 pKa = 6.77GISIRR434 pKa = 11.84LSGGSNNSNCLLNDD448 pKa = 3.41EE449 pKa = 4.48QMGEE453 pKa = 4.24GWSDD457 pKa = 2.68WYY459 pKa = 11.28GAMLTIRR466 pKa = 11.84DD467 pKa = 4.3GDD469 pKa = 3.77TGTDD473 pKa = 3.44PRR475 pKa = 11.84PIGNWLFGNDD485 pKa = 3.42EE486 pKa = 4.59DD487 pKa = 5.05GAGIRR492 pKa = 11.84AFPYY496 pKa = 9.69STDD499 pKa = 3.49LAVNPQTYY507 pKa = 11.02DD508 pKa = 3.59DD509 pKa = 4.32IKK511 pKa = 10.29PTFGPHH517 pKa = 6.32PLGSIWATMLWEE529 pKa = 4.29MTWGLIDD536 pKa = 4.68DD537 pKa = 5.03HH538 pKa = 8.32GFDD541 pKa = 3.95PDD543 pKa = 4.44FYY545 pKa = 11.23NGSGGNNVALAIVTEE560 pKa = 4.08ALKK563 pKa = 10.49LQPCSPGFVDD573 pKa = 5.87GRR575 pKa = 11.84DD576 pKa = 3.63AILAADD582 pKa = 3.49QALYY586 pKa = 10.66GGQNFCTIWDD596 pKa = 3.84AFSKK600 pKa = 10.58RR601 pKa = 11.84GLGMSADD608 pKa = 3.51QGSSNNRR615 pKa = 11.84TDD617 pKa = 2.95GTEE620 pKa = 4.21AFDD623 pKa = 4.6SPSTMLTTPEE633 pKa = 4.32TIFCITDD640 pKa = 3.29SNQNLSGGLPLGGVYY655 pKa = 10.25SGSGVTDD662 pKa = 3.18SGDD665 pKa = 3.45GEE667 pKa = 4.83NYY669 pKa = 9.35TFSPLEE675 pKa = 3.97AGIGVHH681 pKa = 6.62TITYY685 pKa = 9.63AAEE688 pKa = 4.33SDD690 pKa = 3.8CSSANQASDD699 pKa = 3.54TIEE702 pKa = 4.18VKK704 pKa = 10.77DD705 pKa = 3.88EE706 pKa = 4.03NPIIEE711 pKa = 4.44CQNISVALDD720 pKa = 3.42EE721 pKa = 6.12DD722 pKa = 4.16GTATIIPQDD731 pKa = 2.91IVTNFEE737 pKa = 3.9ASDD740 pKa = 4.62GYY742 pKa = 9.52TLDD745 pKa = 3.4QSGTFAPEE753 pKa = 4.25NIDD756 pKa = 5.49DD757 pKa = 4.08VDD759 pKa = 4.07TQISLGDD766 pKa = 3.73DD767 pKa = 3.3QVASGLSLGFDD778 pKa = 3.66FNFYY782 pKa = 9.16GTDD785 pKa = 3.36YY786 pKa = 10.9SAFGISSNGYY796 pKa = 10.42LSFSDD801 pKa = 4.6NFDD804 pKa = 3.66SGCCSGQTLPNSNLPNNLIALGWTDD829 pKa = 4.84LNPGNGGSISYY840 pKa = 8.33ATIGSAPNRR849 pKa = 11.84ILIVQFDD856 pKa = 4.21NIQNYY861 pKa = 9.46GDD863 pKa = 3.55SSKK866 pKa = 9.89TVTSQIKK873 pKa = 10.16LFEE876 pKa = 4.55GSNHH880 pKa = 6.19IEE882 pKa = 3.58IHH884 pKa = 5.53SEE886 pKa = 3.82NVEE889 pKa = 4.33GNNMTQGIEE898 pKa = 4.06NTDD901 pKa = 2.77GSMALPVPGRR911 pKa = 11.84NSEE914 pKa = 4.25TLSLSNDD921 pKa = 3.12FVSFIPNTGSFSDD934 pKa = 3.58NCGLEE939 pKa = 4.34TIVTLDD945 pKa = 3.8INSFDD950 pKa = 4.95CNDD953 pKa = 3.53LGEE956 pKa = 4.91NIVTATATDD965 pKa = 3.59TAGNLASCMATVTVTSDD982 pKa = 3.27LDD984 pKa = 3.75VTFSDD989 pKa = 3.86VDD991 pKa = 3.56EE992 pKa = 4.94EE993 pKa = 4.52FCIDD997 pKa = 3.55QNPITGLNGGLPVGGFYY1014 pKa = 10.54SGNGVTDD1021 pKa = 4.25DD1022 pKa = 4.28GNGEE1026 pKa = 4.27TFTFNPSVAGEE1037 pKa = 4.25GTTTITYY1044 pKa = 10.59NGDD1047 pKa = 3.5NSCSAEE1053 pKa = 3.85GSATIDD1059 pKa = 3.59VEE1061 pKa = 4.25IQSAIPVLDD1070 pKa = 4.25CQDD1073 pKa = 2.62ITVPLNIDD1081 pKa = 3.47GLVTITVEE1089 pKa = 4.38DD1090 pKa = 3.77VFGADD1095 pKa = 4.58SIPVDD1100 pKa = 3.55NCVGEE1105 pKa = 4.3PLTFSISPNTFGCDD1119 pKa = 3.02DD1120 pKa = 3.63VGEE1123 pKa = 4.61NIVTVTATDD1132 pKa = 3.39NAGNSQTCTSTVTVEE1147 pKa = 4.21DD1148 pKa = 3.69TAPLQFEE1155 pKa = 5.06CINSIEE1161 pKa = 4.18LVLDD1165 pKa = 3.89EE1166 pKa = 5.0NQTATIKK1173 pKa = 10.75EE1174 pKa = 3.88DD1175 pKa = 4.84DD1176 pKa = 3.93IFEE1179 pKa = 4.65SLPQDD1184 pKa = 3.51SCDD1187 pKa = 3.19NGYY1190 pKa = 10.39SVSISQSNFNCDD1202 pKa = 3.39DD1203 pKa = 4.24LGNSSVEE1210 pKa = 4.06EE1211 pKa = 3.78ILINGSFEE1219 pKa = 4.09NGLTGWTSTVEE1230 pKa = 4.81DD1231 pKa = 4.73GLDD1234 pKa = 3.75DD1235 pKa = 5.33DD1236 pKa = 5.7SPGSCEE1242 pKa = 4.16QAWKK1246 pKa = 9.62PLEE1249 pKa = 4.57DD1250 pKa = 4.12SSTICCCVDD1259 pKa = 3.34NIVPTEE1265 pKa = 4.04GASASFTSFDD1275 pKa = 3.46GQAGTKK1281 pKa = 10.17YY1282 pKa = 10.27ILEE1285 pKa = 4.02QSFIATSGGNAILSFDD1301 pKa = 3.21WVAEE1305 pKa = 4.27FNLNTADD1312 pKa = 3.33IDD1314 pKa = 3.77RR1315 pKa = 11.84SFEE1318 pKa = 3.96VGIFDD1323 pKa = 4.22TNGNLIEE1330 pKa = 4.45TIYY1333 pKa = 10.53TEE1335 pKa = 4.63SITAGEE1341 pKa = 4.32TTSINSSLSFDD1352 pKa = 3.29ISSVLSLLDD1361 pKa = 3.54GEE1363 pKa = 4.6EE1364 pKa = 4.24VVLKK1368 pKa = 8.01FTATIPEE1375 pKa = 4.47TLSGPSKK1382 pKa = 11.25AMLDD1386 pKa = 3.75NVSLIVDD1393 pKa = 4.05SSTIPVEE1400 pKa = 3.94ITVTDD1405 pKa = 3.47AFGNSNTCIVPVTVTDD1421 pKa = 4.18PNEE1424 pKa = 3.87EE1425 pKa = 4.9CILSTDD1431 pKa = 4.26DD1432 pKa = 4.92RR1433 pKa = 11.84FLEE1436 pKa = 4.46NSLSLYY1442 pKa = 9.43PNPADD1447 pKa = 3.41TSFTITWNQDD1457 pKa = 3.0VTVEE1461 pKa = 3.96RR1462 pKa = 11.84LEE1464 pKa = 4.1ILDD1467 pKa = 3.69MTGKK1471 pKa = 10.84LILQRR1476 pKa = 11.84RR1477 pKa = 11.84INISDD1482 pKa = 3.56RR1483 pKa = 11.84QTLVGVSSLSSGVYY1497 pKa = 8.77FVKK1500 pKa = 10.69VSSKK1504 pKa = 10.56NGQSIKK1510 pKa = 10.67KK1511 pKa = 9.81LLVKK1515 pKa = 10.68

Molecular weight: 162.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4IJJ4|K4IJJ4_PSYTT Uncharacterized protein OS=Psychroflexus torquis (strain ATCC 700755 / ACAM 623) OX=313595 GN=P700755_002527 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNKK10 pKa = 8.92KK11 pKa = 9.95RR12 pKa = 11.84KK13 pKa = 8.04NKK15 pKa = 9.86HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.13RR21 pKa = 11.84MSSVNGRR28 pKa = 11.84KK29 pKa = 8.49VLKK32 pKa = 10.12RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.65LSVSSEE47 pKa = 3.37MSLRR51 pKa = 11.84KK52 pKa = 9.51RR53 pKa = 3.5

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3457

0

3457

1142495

31

4408

330.5

37.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.702 ± 0.042

0.688 ± 0.012

5.788 ± 0.033

6.9 ± 0.037

5.517 ± 0.032

6.078 ± 0.044

1.761 ± 0.023

8.089 ± 0.045

7.97 ± 0.064

9.661 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.127 ± 0.019

6.026 ± 0.049

3.263 ± 0.023

3.543 ± 0.025

3.456 ± 0.029

7.099 ± 0.036

5.52 ± 0.042

5.848 ± 0.034

1.024 ± 0.014

3.939 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski