Amino acid dipepetide frequency for Psychroflexus torquis (strain ATCC 700755 / ACAM 623)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.311AlaAla: 3.311 ± 0.067	0.509AlaCys: 0.509 ± 0.024	2.982AlaAsp: 2.982 ± 0.054	3.752AlaGlu: 3.752 ± 0.062	3.18AlaPhe: 3.18 ± 0.063	3.655AlaGly: 3.655 ± 0.075	1.004AlaHis: 1.004 ± 0.028	5.026AlaIle: 5.026 ± 0.071	4.444AlaLys: 4.444 ± 0.086	5.842AlaLeu: 5.842 ± 0.078	1.36AlaMet: 1.36 ± 0.039	3.034AlaAsn: 3.034 ± 0.057	1.56AlaPro: 1.56 ± 0.043	2.232AlaGln: 2.232 ± 0.048	1.809AlaArg: 1.809 ± 0.04	3.963AlaSer: 3.963 ± 0.06	3.058AlaThr: 3.058 ± 0.07	3.422AlaVal: 3.422 ± 0.063	0.523AlaTrp: 0.523 ± 0.018	2.352AlaTyr: 2.352 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.337CysAla: 0.337 ± 0.016	0.094CysCys: 0.094 ± 0.011	0.398CysAsp: 0.398 ± 0.019	0.484CysGlu: 0.484 ± 0.021	0.428CysPhe: 0.428 ± 0.023	0.545CysGly: 0.545 ± 0.026	0.158CysHis: 0.158 ± 0.011	0.537CysIle: 0.537 ± 0.022	0.482CysLys: 0.482 ± 0.021	0.633CysLeu: 0.633 ± 0.023	0.129CysMet: 0.129 ± 0.012	0.362CysAsn: 0.362 ± 0.02	0.278CysPro: 0.278 ± 0.019	0.223CysGln: 0.223 ± 0.015	0.196CysArg: 0.196 ± 0.014	0.526CysSer: 0.526 ± 0.024	0.343CysThr: 0.343 ± 0.017	0.418CysVal: 0.418 ± 0.019	0.067CysTrp: 0.067 ± 0.008	0.24CysTyr: 0.24 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.302AspAla: 3.302 ± 0.072	0.397AspCys: 0.397 ± 0.021	3.016AspAsp: 3.016 ± 0.07	4.048AspGlu: 4.048 ± 0.065	4.116AspPhe: 4.116 ± 0.064	3.434AspGly: 3.434 ± 0.084	0.986AspHis: 0.986 ± 0.029	4.812AspIle: 4.812 ± 0.071	4.261AspLys: 4.261 ± 0.085	5.984AspLeu: 5.984 ± 0.082	1.207AspMet: 1.207 ± 0.045	3.049AspAsn: 3.049 ± 0.062	1.776AspPro: 1.776 ± 0.078	1.921AspGln: 1.921 ± 0.037	1.948AspArg: 1.948 ± 0.042	3.632AspSer: 3.632 ± 0.064	2.657AspThr: 2.657 ± 0.053	3.576AspVal: 3.576 ± 0.075	0.789AspTrp: 0.789 ± 0.042	2.964AspTyr: 2.964 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.202GluAla: 4.202 ± 0.072	0.321GluCys: 0.321 ± 0.019	4.248GluAsp: 4.248 ± 0.068	4.979GluGlu: 4.979 ± 0.091	3.563GluPhe: 3.563 ± 0.062	3.806GluGly: 3.806 ± 0.063	1.183GluHis: 1.183 ± 0.038	5.869GluIle: 5.869 ± 0.088	5.892GluLys: 5.892 ± 0.098	6.653GluLeu: 6.653 ± 0.095	1.593GluMet: 1.593 ± 0.039	4.953GluAsn: 4.953 ± 0.077	1.544GluPro: 1.544 ± 0.049	2.321GluGln: 2.321 ± 0.045	2.453GluArg: 2.453 ± 0.049	4.157GluSer: 4.157 ± 0.062	3.762GluThr: 3.762 ± 0.053	4.597GluVal: 4.597 ± 0.065	0.651GluTrp: 0.651 ± 0.027	2.255GluTyr: 2.255 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.894PheAla: 2.894 ± 0.053	0.439PheCys: 0.439 ± 0.019	3.569PheAsp: 3.569 ± 0.072	4.029PheGlu: 4.029 ± 0.059	2.873PhePhe: 2.873 ± 0.058	3.709PheGly: 3.709 ± 0.06	0.838PheHis: 0.838 ± 0.027	4.253PheIle: 4.253 ± 0.069	4.253PheLys: 4.253 ± 0.079	4.997PheLeu: 4.997 ± 0.088	1.142PheMet: 1.142 ± 0.035	3.576PheAsn: 3.576 ± 0.076	1.781PhePro: 1.781 ± 0.05	1.893PheGln: 1.893 ± 0.042	1.764PheArg: 1.764 ± 0.041	4.387PheSer: 4.387 ± 0.069	3.062PheThr: 3.062 ± 0.057	2.866PheVal: 2.866 ± 0.048	0.632PheTrp: 0.632 ± 0.026	2.221PheTyr: 2.221 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	3.661GlyAla: 3.661 ± 0.068	0.44GlyCys: 0.44 ± 0.02	3.455GlyAsp: 3.455 ± 0.09	3.749GlyGlu: 3.749 ± 0.064	3.856GlyPhe: 3.856 ± 0.073	4.188GlyGly: 4.188 ± 0.079	1.12GlyHis: 1.12 ± 0.04	5.302GlyIle: 5.302 ± 0.068	4.531GlyLys: 4.531 ± 0.079	5.802GlyLeu: 5.802 ± 0.076	1.452GlyMet: 1.452 ± 0.034	3.407GlyAsn: 3.407 ± 0.083	1.231GlyPro: 1.231 ± 0.035	1.983GlyGln: 1.983 ± 0.049	2.014GlyArg: 2.014 ± 0.043	3.891GlySer: 3.891 ± 0.066	3.516GlyThr: 3.516 ± 0.093	4.025GlyVal: 4.025 ± 0.071	0.67GlyTrp: 0.67 ± 0.027	2.49GlyTyr: 2.49 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	0.807HisAla: 0.807 ± 0.024	0.159HisCys: 0.159 ± 0.013	0.768HisAsp: 0.768 ± 0.028	0.902HisGlu: 0.902 ± 0.027	1.136HisPhe: 1.136 ± 0.032	0.952HisGly: 0.952 ± 0.033	0.498HisHis: 0.498 ± 0.023	1.51HisIle: 1.51 ± 0.045	1.381HisLys: 1.381 ± 0.036	1.94HisLeu: 1.94 ± 0.046	0.327HisMet: 0.327 ± 0.016	0.895HisAsn: 0.895 ± 0.03	0.893HisPro: 0.893 ± 0.029	0.792HisGln: 0.792 ± 0.028	0.686HisArg: 0.686 ± 0.023	1.145HisSer: 1.145 ± 0.031	0.898HisThr: 0.898 ± 0.027	0.882HisVal: 0.882 ± 0.028	0.203HisTrp: 0.203 ± 0.013	0.839HisTyr: 0.839 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.044IleAla: 5.044 ± 0.072	0.612IleCys: 0.612 ± 0.023	5.315IleAsp: 5.315 ± 0.077	6.053IleGlu: 6.053 ± 0.081	3.899IlePhe: 3.899 ± 0.062	4.997IleGly: 4.997 ± 0.073	1.406IleHis: 1.406 ± 0.041	6.192IleIle: 6.192 ± 0.093	6.045IleLys: 6.045 ± 0.092	7.837IleLeu: 7.837 ± 0.111	1.377IleMet: 1.377 ± 0.031	4.755IleAsn: 4.755 ± 0.076	3.175IlePro: 3.175 ± 0.058	2.868IleGln: 2.868 ± 0.052	2.605IleArg: 2.605 ± 0.052	6.231IleSer: 6.231 ± 0.078	4.386IleThr: 4.386 ± 0.067	4.446IleVal: 4.446 ± 0.076	0.726IleTrp: 0.726 ± 0.026	2.923IleTyr: 2.923 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.925LysAla: 4.925 ± 0.08	0.378LysCys: 0.378 ± 0.018	4.752LysAsp: 4.752 ± 0.081	6.243LysGlu: 6.243 ± 0.106	3.358LysPhe: 3.358 ± 0.057	4.352LysGly: 4.352 ± 0.071	1.451LysHis: 1.451 ± 0.04	6.282LysIle: 6.282 ± 0.093	6.667LysLys: 6.667 ± 0.12	7.19LysLeu: 7.19 ± 0.096	1.943LysMet: 1.943 ± 0.044	5.117LysAsn: 5.117 ± 0.083	2.46LysPro: 2.46 ± 0.054	2.768LysGln: 2.768 ± 0.057	3.169LysArg: 3.169 ± 0.07	5.411LysSer: 5.411 ± 0.089	5.07LysThr: 5.07 ± 0.082	4.586LysVal: 4.586 ± 0.078	0.74LysTrp: 0.74 ± 0.026	2.835LysTyr: 2.835 ± 0.058	0.0LysXaa: 0.0 ± 0.0
Leu	5.428LeuAla: 5.428 ± 0.073	0.645LeuCys: 0.645 ± 0.024	5.527LeuAsp: 5.527 ± 0.066	6.685LeuGlu: 6.685 ± 0.085	5.039LeuPhe: 5.039 ± 0.087	5.828LeuGly: 5.828 ± 0.072	1.527LeuHis: 1.527 ± 0.038	7.778LeuIle: 7.778 ± 0.105	8.833LeuLys: 8.833 ± 0.12	8.761LeuLeu: 8.761 ± 0.129	2.088LeuMet: 2.088 ± 0.047	6.226LeuAsn: 6.226 ± 0.082	3.459LeuPro: 3.459 ± 0.054	3.309LeuGln: 3.309 ± 0.059	3.295LeuArg: 3.295 ± 0.06	7.644LeuSer: 7.644 ± 0.09	5.096LeuThr: 5.096 ± 0.069	5.468LeuVal: 5.468 ± 0.08	0.768LeuTrp: 0.768 ± 0.024	3.204LeuTyr: 3.204 ± 0.074	0.0LeuXaa: 0.0 ± 0.0
Met	1.402MetAla: 1.402 ± 0.039	0.144MetCys: 0.144 ± 0.012	1.191MetAsp: 1.191 ± 0.035	1.373MetGlu: 1.373 ± 0.037	0.87MetPhe: 0.87 ± 0.037	1.316MetGly: 1.316 ± 0.042	0.345MetHis: 0.345 ± 0.017	1.561MetIle: 1.561 ± 0.037	2.24MetLys: 2.24 ± 0.045	1.842MetLeu: 1.842 ± 0.041	0.551MetMet: 0.551 ± 0.022	1.258MetAsn: 1.258 ± 0.033	0.79MetPro: 0.79 ± 0.037	0.762MetGln: 0.762 ± 0.023	0.865MetArg: 0.865 ± 0.028	1.506MetSer: 1.506 ± 0.037	1.203MetThr: 1.203 ± 0.033	1.249MetVal: 1.249 ± 0.037	0.158MetTrp: 0.158 ± 0.012	0.649MetTyr: 0.649 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.474AsnAla: 3.474 ± 0.059	0.411AsnCys: 0.411 ± 0.021	3.144AsnAsp: 3.144 ± 0.062	3.747AsnGlu: 3.747 ± 0.067	3.473AsnPhe: 3.473 ± 0.062	3.647AsnGly: 3.647 ± 0.12	1.022AsnHis: 1.022 ± 0.033	4.619AsnIle: 4.619 ± 0.065	4.308AsnLys: 4.308 ± 0.074	6.013AsnLeu: 6.013 ± 0.087	1.218AsnMet: 1.218 ± 0.033	3.341AsnAsn: 3.341 ± 0.069	2.673AsnPro: 2.673 ± 0.056	2.399AsnGln: 2.399 ± 0.059	2.212AsnArg: 2.212 ± 0.041	4.414AsnSer: 4.414 ± 0.076	3.446AsnThr: 3.446 ± 0.062	3.107AsnVal: 3.107 ± 0.058	0.8AsnTrp: 0.8 ± 0.033	2.796AsnTyr: 2.796 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	1.492ProAla: 1.492 ± 0.04	0.193ProCys: 0.193 ± 0.015	2.046ProAsp: 2.046 ± 0.054	2.721ProGlu: 2.721 ± 0.055	1.821ProPhe: 1.821 ± 0.04	1.737ProGly: 1.737 ± 0.045	0.552ProHis: 0.552 ± 0.021	2.64ProIle: 2.64 ± 0.052	2.746ProLys: 2.746 ± 0.051	2.959ProLeu: 2.959 ± 0.064	0.645ProMet: 0.645 ± 0.023	2.22ProAsn: 2.22 ± 0.05	0.786ProPro: 0.786 ± 0.032	1.168ProGln: 1.168 ± 0.038	0.962ProArg: 0.962 ± 0.031	2.364ProSer: 2.364 ± 0.053	1.845ProThr: 1.845 ± 0.076	2.106ProVal: 2.106 ± 0.045	0.314ProTrp: 0.314 ± 0.018	1.314ProTyr: 1.314 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	1.887GlnAla: 1.887 ± 0.048	0.17GlnCys: 0.17 ± 0.012	1.992GlnAsp: 1.992 ± 0.04	2.319GlnGlu: 2.319 ± 0.048	1.833GlnPhe: 1.833 ± 0.046	1.828GlnGly: 1.828 ± 0.05	0.669GlnHis: 0.669 ± 0.021	2.834GlnIle: 2.834 ± 0.051	3.105GlnLys: 3.105 ± 0.063	3.681GlnLeu: 3.681 ± 0.064	0.803GlnMet: 0.803 ± 0.027	2.544GlnAsn: 2.544 ± 0.056	1.166GlnPro: 1.166 ± 0.045	1.386GlnGln: 1.386 ± 0.037	1.358GlnArg: 1.358 ± 0.035	2.14GlnSer: 2.14 ± 0.046	2.048GlnThr: 2.048 ± 0.076	2.127GlnVal: 2.127 ± 0.04	0.367GlnTrp: 0.367 ± 0.018	1.168GlnTyr: 1.168 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	1.965ArgAla: 1.965 ± 0.047	0.214ArgCys: 0.214 ± 0.016	1.891ArgAsp: 1.891 ± 0.046	2.289ArgGlu: 2.289 ± 0.046	2.011ArgPhe: 2.011 ± 0.044	1.922ArgGly: 1.922 ± 0.047	0.644ArgHis: 0.644 ± 0.023	2.858ArgIle: 2.858 ± 0.048	2.755ArgLys: 2.755 ± 0.055	3.485ArgLeu: 3.485 ± 0.058	0.829ArgMet: 0.829 ± 0.03	2.019ArgAsn: 2.019 ± 0.045	1.116ArgPro: 1.116 ± 0.029	1.206ArgGln: 1.206 ± 0.032	1.364ArgArg: 1.364 ± 0.037	2.14ArgSer: 2.14 ± 0.052	1.811ArgThr: 1.811 ± 0.039	2.123ArgVal: 2.123 ± 0.046	0.388ArgTrp: 0.388 ± 0.018	1.526ArgTyr: 1.526 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.648SerAla: 3.648 ± 0.063	0.602SerCys: 0.602 ± 0.025	3.739SerAsp: 3.739 ± 0.07	4.827SerGlu: 4.827 ± 0.07	4.179SerPhe: 4.179 ± 0.077	4.555SerGly: 4.555 ± 0.074	1.226SerHis: 1.226 ± 0.036	6.176SerIle: 6.176 ± 0.075	5.826SerLys: 5.826 ± 0.087	6.91SerLeu: 6.91 ± 0.087	1.379SerMet: 1.379 ± 0.035	4.004SerAsn: 4.004 ± 0.08	2.214SerPro: 2.214 ± 0.047	2.607SerGln: 2.607 ± 0.054	2.381SerArg: 2.381 ± 0.045	5.312SerSer: 5.312 ± 0.093	3.838SerThr: 3.838 ± 0.067	3.959SerVal: 3.959 ± 0.061	0.739SerTrp: 0.739 ± 0.027	2.871SerTyr: 2.871 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	3.191ThrAla: 3.191 ± 0.062	0.319ThrCys: 0.319 ± 0.019	3.288ThrAsp: 3.288 ± 0.093	3.58ThrGlu: 3.58 ± 0.061	2.936ThrPhe: 2.936 ± 0.055	3.733ThrGly: 3.733 ± 0.106	1.058ThrHis: 1.058 ± 0.034	4.365ThrIle: 4.365 ± 0.068	3.825ThrLys: 3.825 ± 0.063	5.501ThrLeu: 5.501 ± 0.08	0.966ThrMet: 0.966 ± 0.035	3.098ThrAsn: 3.098 ± 0.067	2.198ThrPro: 2.198 ± 0.047	1.954ThrGln: 1.954 ± 0.056	1.659ThrArg: 1.659 ± 0.041	4.233ThrSer: 4.233 ± 0.068	3.295ThrThr: 3.295 ± 0.125	3.148ThrVal: 3.148 ± 0.055	0.496ThrTrp: 0.496 ± 0.02	2.356ThrTyr: 2.356 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	3.348ValAla: 3.348 ± 0.06	0.453ValCys: 0.453 ± 0.021	3.67ValAsp: 3.67 ± 0.065	4.146ValGlu: 4.146 ± 0.064	3.463ValPhe: 3.463 ± 0.069	3.564ValGly: 3.564 ± 0.065	0.959ValHis: 0.959 ± 0.029	4.697ValIle: 4.697 ± 0.072	4.192ValLys: 4.192 ± 0.069	5.706ValLeu: 5.706 ± 0.082	1.265ValMet: 1.265 ± 0.03	3.372ValAsn: 3.372 ± 0.057	1.814ValPro: 1.814 ± 0.039	1.687ValGln: 1.687 ± 0.04	1.889ValArg: 1.889 ± 0.045	4.398ValSer: 4.398 ± 0.068	3.221ValThr: 3.221 ± 0.081	3.747ValVal: 3.747 ± 0.065	0.565ValTrp: 0.565 ± 0.025	2.322ValTyr: 2.322 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.527TrpAla: 0.527 ± 0.023	0.097TrpCys: 0.097 ± 0.009	0.632TrpAsp: 0.632 ± 0.033	0.661TrpGlu: 0.661 ± 0.023	0.577TrpPhe: 0.577 ± 0.022	0.591TrpGly: 0.591 ± 0.022	0.197TrpHis: 0.197 ± 0.016	0.837TrpIle: 0.837 ± 0.03	0.779TrpLys: 0.779 ± 0.029	0.973TrpLeu: 0.973 ± 0.032	0.319TrpMet: 0.319 ± 0.017	0.678TrpAsn: 0.678 ± 0.022	0.243TrpPro: 0.243 ± 0.015	0.391TrpGln: 0.391 ± 0.018	0.39TrpArg: 0.39 ± 0.021	0.688TrpSer: 0.688 ± 0.036	0.528TrpThr: 0.528 ± 0.022	0.592TrpVal: 0.592 ± 0.026	0.172TrpTrp: 0.172 ± 0.013	0.366TrpTyr: 0.366 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.172TyrAla: 2.172 ± 0.045	0.282TyrCys: 0.282 ± 0.017	2.258TyrAsp: 2.258 ± 0.053	2.412TyrGlu: 2.412 ± 0.05	2.564TyrPhe: 2.564 ± 0.054	2.425TyrGly: 2.425 ± 0.057	0.77TyrHis: 0.77 ± 0.027	2.745TyrIle: 2.745 ± 0.053	3.132TyrLys: 3.132 ± 0.059	3.896TyrLeu: 3.896 ± 0.061	0.679TyrMet: 0.679 ± 0.023	2.358TyrAsn: 2.358 ± 0.047	1.475TyrPro: 1.475 ± 0.04	1.555TyrGln: 1.555 ± 0.043	1.538TyrArg: 1.538 ± 0.041	2.811TyrSer: 2.811 ± 0.049	2.116TyrThr: 2.116 ± 0.046	2.033TyrVal: 2.033 ± 0.043	0.468TyrTrp: 0.468 ± 0.019	1.704TyrTyr: 1.704 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3457 proteins (1142495 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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