Baboon orthoreovirus
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q918V6|Q918V6_9REOV Membrane fusion protein p15 OS=Baboon orthoreovirus OX=75888 PE=1 SV=1
MM1 pKa = 7.14 MEE3 pKa = 4.17 EE4 pKa = 4.0 TQMEE8 pKa = 4.7 VSTFEE13 pKa = 4.04 WPKK16 pKa = 11.25 SLDD19 pKa = 3.1 EE20 pKa = 4.34 SLQVLCNEE28 pKa = 4.81 LKK30 pKa = 10.94 GKK32 pKa = 7.59 TEE34 pKa = 3.9 WQDD37 pKa = 4.92 DD38 pKa = 4.03 MEE40 pKa = 6.18 DD41 pKa = 3.16 WMPYY45 pKa = 8.04 WIYY48 pKa = 10.27 MKK50 pKa = 10.3 HH51 pKa = 6.58 DD52 pKa = 5.3 GIAISQSRR60 pKa = 11.84 YY61 pKa = 10.6 SLLQQLAVWVWKK73 pKa = 10.86 CFDD76 pKa = 4.5 FDD78 pKa = 3.67 MCVYY82 pKa = 10.56 NIWTTWLVKK91 pKa = 9.79 HH92 pKa = 6.07 ACSRR96 pKa = 11.84 CPEE99 pKa = 4.16 FDD101 pKa = 5.99 DD102 pKa = 3.7 EE103 pKa = 5.0 AFWSGVPTIIKK114 pKa = 9.98 LVIRR118 pKa = 11.84 KK119 pKa = 7.22 TMHH122 pKa = 6.42 RR123 pKa = 11.84 YY124 pKa = 9.61 AYY126 pKa = 10.78 LDD128 pKa = 4.08 DD129 pKa = 4.05 STLADD134 pKa = 3.5 LTEE137 pKa = 4.15 QVGLL141 pKa = 3.99
Molecular weight: 16.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.35
IPC2_protein 4.495
IPC_protein 4.418
Toseland 4.253
ProMoST 4.546
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.266
Rodwell 4.253
Grimsley 4.164
Solomon 4.368
Lehninger 4.329
Nozaki 4.495
DTASelect 4.673
Thurlkill 4.266
EMBOSS 4.291
Sillero 4.533
Patrickios 3.16
IPC_peptide 4.38
IPC2_peptide 4.52
IPC2.peptide.svr19 4.47
Protein with the highest isoelectric point:
>tr|Q918V6|Q918V6_9REOV Membrane fusion protein p15 OS=Baboon orthoreovirus OX=75888 PE=1 SV=1
MM1 pKa = 7.11 GQRR4 pKa = 11.84 HH5 pKa = 6.56 SIVQPPAPPPNAFVEE20 pKa = 4.78 IVSSSTGIIIAVGIFAFIFSFLYY43 pKa = 10.67 KK44 pKa = 10.62 LLQWYY49 pKa = 8.34 NRR51 pKa = 11.84 KK52 pKa = 9.64 SKK54 pKa = 10.29 NKK56 pKa = 9.23 KK57 pKa = 8.49 RR58 pKa = 11.84 KK59 pKa = 7.21 EE60 pKa = 3.87 QIRR63 pKa = 11.84 EE64 pKa = 3.98 QIEE67 pKa = 4.39 LGLLSYY73 pKa = 10.67 GAGVASLPLLNVIAHH88 pKa = 6.24 NPGSVISATPIYY100 pKa = 9.99 KK101 pKa = 10.33 GPCTGVPNSRR111 pKa = 11.84 LLQITSGTAEE121 pKa = 4.06 EE122 pKa = 4.05 NTRR125 pKa = 11.84 ILNHH129 pKa = 7.2 DD130 pKa = 3.53 GRR132 pKa = 11.84 NPDD135 pKa = 2.83 GSINVV140 pKa = 3.56
Molecular weight: 15.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.103
IPC2_protein 9.224
IPC_protein 9.253
Toseland 9.867
ProMoST 9.575
Dawson 10.087
Bjellqvist 9.78
Wikipedia 10.262
Rodwell 10.452
Grimsley 10.16
Solomon 10.145
Lehninger 10.101
Nozaki 9.882
DTASelect 9.765
Thurlkill 9.926
EMBOSS 10.277
Sillero 10.014
Patrickios 10.058
IPC_peptide 10.131
IPC2_peptide 8.434
IPC2.peptide.svr19 8.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
7236
140
1284
657.8
74.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.818 ± 0.559
1.562 ± 0.169
6.191 ± 0.344
4.63 ± 0.357
3.994 ± 0.177
4.961 ± 0.337
2.64 ± 0.239
7.2 ± 0.299
4.671 ± 0.46
9.881 ± 0.299
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.054 ± 0.333
5.915 ± 0.305
4.768 ± 0.426
3.704 ± 0.24
4.92 ± 0.216
8.25 ± 0.311
5.735 ± 0.187
6.233 ± 0.222
1.423 ± 0.173
4.45 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here