Planktotalea frisia
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4128 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L9NW35|A0A1L9NW35_9RHOB Mycothiol acetyltransferase OS=Planktotalea frisia OX=696762 GN=mshD_3 PE=4 SV=1
MM1 pKa = 6.82 MRR3 pKa = 11.84 AGALCLALVPAPAMADD19 pKa = 3.46 FTEE22 pKa = 4.57 SNLLAIFYY30 pKa = 10.25 HH31 pKa = 6.07 EE32 pKa = 5.11 LGHH35 pKa = 7.26 AVIHH39 pKa = 5.87 QMDD42 pKa = 3.9 VPIFGQEE49 pKa = 3.34 EE50 pKa = 3.95 DD51 pKa = 3.86 AADD54 pKa = 3.95 ALSILLIDD62 pKa = 4.4 ALYY65 pKa = 9.73 EE66 pKa = 4.21 AEE68 pKa = 4.13 DD69 pKa = 4.43 AEE71 pKa = 4.81 VIAYY75 pKa = 9.74 DD76 pKa = 3.94 SANLFWAEE84 pKa = 4.04 AQNEE88 pKa = 4.04 PAFWDD93 pKa = 3.2 THH95 pKa = 6.94 GPDD98 pKa = 3.06 EE99 pKa = 4.1 QRR101 pKa = 11.84 YY102 pKa = 8.56 FNTVCLFYY110 pKa = 10.96 GADD113 pKa = 3.39 PDD115 pKa = 3.84 GRR117 pKa = 11.84 EE118 pKa = 3.97 EE119 pKa = 3.93 FAQDD123 pKa = 3.51 LEE125 pKa = 4.49 LPEE128 pKa = 4.33 EE129 pKa = 4.09 RR130 pKa = 11.84 AEE132 pKa = 4.22 GCEE135 pKa = 4.94 DD136 pKa = 3.79 EE137 pKa = 5.03 FALAADD143 pKa = 3.97 SWHH146 pKa = 6.34 SVLDD150 pKa = 3.88 GMSANSGLTGQLVLNGSVAMEE171 pKa = 5.08 LISHH175 pKa = 7.06 EE176 pKa = 4.18 IKK178 pKa = 10.79 ALNAEE183 pKa = 4.73 FKK185 pKa = 9.46 WPRR188 pKa = 11.84 NITVTLEE195 pKa = 4.08 SCDD198 pKa = 3.75 EE199 pKa = 4.18 ANAFYY204 pKa = 10.99 DD205 pKa = 5.09 LDD207 pKa = 3.95 AQSITMCTEE216 pKa = 3.51 LVDD219 pKa = 3.84 WLDD222 pKa = 4.8 GIATQIAADD231 pKa = 3.5
Molecular weight: 25.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.541
ProMoST 3.846
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.567
Grimsley 3.439
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.859
Patrickios 0.668
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A1L9P060|A0A1L9P060_9RHOB Amino_oxidase domain-containing protein OS=Planktotalea frisia OX=696762 GN=PFRI_08930 PE=4 SV=1
MM1 pKa = 6.92 TRR3 pKa = 11.84 FMAIQTTSRR12 pKa = 11.84 RR13 pKa = 11.84 VVAMAMTAFWAARR26 pKa = 11.84 ATTLSLAKK34 pKa = 9.17 TVRR37 pKa = 11.84 IPFVAMVVATRR48 pKa = 11.84 SLVAVVLTPFVVTGAAILSMVAMALTQSQAMAAQIPFAVAKK89 pKa = 10.03 VRR91 pKa = 11.84 IVLTVMQATII101 pKa = 3.57
Molecular weight: 10.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.398
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.149
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4128
0
4128
1233686
29
2126
298.9
32.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.387 ± 0.044
0.956 ± 0.013
6.033 ± 0.031
5.869 ± 0.038
3.971 ± 0.027
8.38 ± 0.041
2.114 ± 0.023
5.693 ± 0.027
3.938 ± 0.033
9.748 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.995 ± 0.02
3.045 ± 0.02
4.669 ± 0.023
3.312 ± 0.023
5.816 ± 0.035
5.709 ± 0.027
5.587 ± 0.026
7.08 ± 0.035
1.371 ± 0.017
2.327 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here