Arthrobacter sp. SRS-W-1-2016

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S9MI69|A0A1S9MI69_9MICC Ferredoxin OS=Arthrobacter sp. SRS-W-1-2016 OX=1930254 GN=BMF89_00740 PE=4 SV=1
MM1 pKa = 7.1SWHH4 pKa = 6.26VEE6 pKa = 3.43GTYY9 pKa = 10.33FEE11 pKa = 4.92NCNCDD16 pKa = 3.55MVCPCSTSGLTAPADD31 pKa = 3.54NDD33 pKa = 3.8RR34 pKa = 11.84CNVALAFHH42 pKa = 6.85VDD44 pKa = 3.15TGEE47 pKa = 4.12VNGVDD52 pKa = 3.33VGGLTVCVVADD63 pKa = 4.79TPALMSDD70 pKa = 4.27GGWKK74 pKa = 9.9IGVLMDD80 pKa = 4.34AAASPEE86 pKa = 3.9QAEE89 pKa = 4.0ALGAVFGGQLGGPMEE104 pKa = 4.81GLTPLIGEE112 pKa = 4.29MAGMEE117 pKa = 4.73SLEE120 pKa = 3.98MAYY123 pKa = 9.18TEE125 pKa = 5.12DD126 pKa = 3.76GRR128 pKa = 11.84SHH130 pKa = 5.01QVRR133 pKa = 11.84IGSAVDD139 pKa = 3.24MAVEE143 pKa = 4.45DD144 pKa = 4.79FVSPMDD150 pKa = 3.59ATGLGVKK157 pKa = 10.12ISGIGFPADD166 pKa = 3.14TLAAGTASTARR177 pKa = 11.84VDD179 pKa = 3.87VFGMTWDD186 pKa = 3.69NAGKK190 pKa = 10.21NSFSAPFAWSAA201 pKa = 3.17

Molecular weight:
20.63 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S9M2V7|A0A1S9M2V7_9MICC Uncharacterized protein OS=Arthrobacter sp. SRS-W-1-2016 OX=1930254 GN=BMF89_19875 PE=3 SV=1
PP1 pKa = 7.76PPPPPPPPPPPPPPPPPPAPPPRR24 pKa = 11.84PPRR27 pKa = 11.84PPPPPPPPPPPPPPPPPPAPPARR50 pKa = 11.84RR51 pKa = 11.84PPAPPPPRR59 pKa = 11.84PPTPRR64 pKa = 11.84GRR66 pKa = 11.84PPPRR70 pKa = 11.84GGRR73 pKa = 11.84VAKK76 pKa = 10.42VV77 pKa = 2.88

Molecular weight:
7.82 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4129

0

4129

1309132

28

2572

317.1

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.874 ± 0.043

0.63 ± 0.01

5.511 ± 0.032

5.52 ± 0.039

3.291 ± 0.021

9.077 ± 0.038

2.088 ± 0.019

4.568 ± 0.023

2.926 ± 0.024

10.246 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.037 ± 0.013

2.538 ± 0.023

5.346 ± 0.027

3.118 ± 0.022

6.346 ± 0.037

6.187 ± 0.025

5.832 ± 0.032

8.357 ± 0.033

1.446 ± 0.016

2.062 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski