Arthrobacter sp. SRS-W-1-2016
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4129 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S9MI69|A0A1S9MI69_9MICC Ferredoxin OS=Arthrobacter sp. SRS-W-1-2016 OX=1930254 GN=BMF89_00740 PE=4 SV=1
MM1 pKa = 7.1 SWHH4 pKa = 6.26 VEE6 pKa = 3.43 GTYY9 pKa = 10.33 FEE11 pKa = 4.92 NCNCDD16 pKa = 3.55 MVCPCSTSGLTAPADD31 pKa = 3.54 NDD33 pKa = 3.8 RR34 pKa = 11.84 CNVALAFHH42 pKa = 6.85 VDD44 pKa = 3.15 TGEE47 pKa = 4.12 VNGVDD52 pKa = 3.33 VGGLTVCVVADD63 pKa = 4.79 TPALMSDD70 pKa = 4.27 GGWKK74 pKa = 9.9 IGVLMDD80 pKa = 4.34 AAASPEE86 pKa = 3.9 QAEE89 pKa = 4.0 ALGAVFGGQLGGPMEE104 pKa = 4.81 GLTPLIGEE112 pKa = 4.29 MAGMEE117 pKa = 4.73 SLEE120 pKa = 3.98 MAYY123 pKa = 9.18 TEE125 pKa = 5.12 DD126 pKa = 3.76 GRR128 pKa = 11.84 SHH130 pKa = 5.01 QVRR133 pKa = 11.84 IGSAVDD139 pKa = 3.24 MAVEE143 pKa = 4.45 DD144 pKa = 4.79 FVSPMDD150 pKa = 3.59 ATGLGVKK157 pKa = 10.12 ISGIGFPADD166 pKa = 3.14 TLAAGTASTARR177 pKa = 11.84 VDD179 pKa = 3.87 VFGMTWDD186 pKa = 3.69 NAGKK190 pKa = 10.21 NSFSAPFAWSAA201 pKa = 3.17
Molecular weight: 20.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|A0A1S9M2V7|A0A1S9M2V7_9MICC Uncharacterized protein OS=Arthrobacter sp. SRS-W-1-2016 OX=1930254 GN=BMF89_19875 PE=3 SV=1
PP1 pKa = 7.76 PPPPPPPPPPPPPPPPPPAPPPRR24 pKa = 11.84 PPRR27 pKa = 11.84 PPPPPPPPPPPPPPPPPPAPPARR50 pKa = 11.84 RR51 pKa = 11.84 PPAPPPPRR59 pKa = 11.84 PPTPRR64 pKa = 11.84 GRR66 pKa = 11.84 PPPRR70 pKa = 11.84 GGRR73 pKa = 11.84 VAKK76 pKa = 10.42 VV77 pKa = 2.88
Molecular weight: 7.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.504
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.457
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.193
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.167
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4129
0
4129
1309132
28
2572
317.1
33.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.874 ± 0.043
0.63 ± 0.01
5.511 ± 0.032
5.52 ± 0.039
3.291 ± 0.021
9.077 ± 0.038
2.088 ± 0.019
4.568 ± 0.023
2.926 ± 0.024
10.246 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.037 ± 0.013
2.538 ± 0.023
5.346 ± 0.027
3.118 ± 0.022
6.346 ± 0.037
6.187 ± 0.025
5.832 ± 0.032
8.357 ± 0.033
1.446 ± 0.016
2.062 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here