Flexibacter flexilis DSM 6793

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Flexibacteraceae; Flexibacter; Flexibacter flexilis

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3664 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1EG55|A0A1I1EG55_9BACT Uncharacterized protein OS=Flexibacter flexilis DSM 6793 OX=927664 GN=SAMN05421780_101708 PE=4 SV=1
MM1 pKa = 7.51IVTEE5 pKa = 4.2GQSVIDD11 pKa = 3.92AHH13 pKa = 6.3LQTHH17 pKa = 6.7GDD19 pKa = 3.81LNTVVQFCANNGITPTSLLLAGQVLQPLEE48 pKa = 4.28SVEE51 pKa = 4.3DD52 pKa = 3.75SSVTDD57 pKa = 3.52YY58 pKa = 11.3YY59 pKa = 11.21AARR62 pKa = 11.84TFRR65 pKa = 11.84VNSGSAMQSVIEE77 pKa = 4.43PMNDD81 pKa = 2.96FDD83 pKa = 5.5SQDD86 pKa = 3.25FDD88 pKa = 4.03SQDD91 pKa = 3.23FYY93 pKa = 12.19

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1NQK6|A0A1I1NQK6_9BACT 50S ribosomal protein L20 OS=Flexibacter flexilis DSM 6793 OX=927664 GN=rplT PE=3 SV=1
MM1 pKa = 7.77AEE3 pKa = 4.0LSSTEE8 pKa = 4.0SLATLSRR15 pKa = 11.84PLATLSRR22 pKa = 11.84PLATLSRR29 pKa = 11.84PLATLSRR36 pKa = 11.84PLAILSRR43 pKa = 11.84PLTTLSRR50 pKa = 11.84PLASLSRR57 pKa = 11.84PLASLSRR64 pKa = 11.84PLTTLSRR71 pKa = 11.84PLTTLSEE78 pKa = 4.26PLASLSRR85 pKa = 11.84PFASLSQPLASLSQPLATLSRR106 pKa = 11.84PLATLSRR113 pKa = 11.84PLTTLSRR120 pKa = 11.84PLTTLSRR127 pKa = 11.84PLTTLSRR134 pKa = 11.84PLTTLSRR141 pKa = 11.84PLTTLSRR148 pKa = 11.84PLTTLSRR155 pKa = 11.84PLTTLSRR162 pKa = 11.84PLTTLSRR169 pKa = 11.84PHH171 pKa = 6.34

Molecular weight: 18.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3664

0

3664

1257853

29

3601

343.3

38.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.271 ± 0.046

0.883 ± 0.014

4.919 ± 0.048

5.778 ± 0.052

4.815 ± 0.032

6.458 ± 0.048

1.847 ± 0.02

6.644 ± 0.037

6.461 ± 0.052

9.54 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.023

5.537 ± 0.042

3.725 ± 0.027

4.395 ± 0.038

3.985 ± 0.033

6.428 ± 0.041

6.197 ± 0.066

6.603 ± 0.034

1.194 ± 0.016

4.098 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski