Amino acid dipepetide frequency for Flexibacter flexilis DSM 6793

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.086AlaAla: 8.086 ± 0.124	0.784AlaCys: 0.784 ± 0.029	4.591AlaAsp: 4.591 ± 0.086	4.957AlaGlu: 4.957 ± 0.085	3.712AlaPhe: 3.712 ± 0.057	5.631AlaGly: 5.631 ± 0.092	1.502AlaHis: 1.502 ± 0.04	5.358AlaIle: 5.358 ± 0.076	4.978AlaLys: 4.978 ± 0.084	7.357AlaLeu: 7.357 ± 0.091	1.875AlaMet: 1.875 ± 0.044	4.356AlaAsn: 4.356 ± 0.071	2.77AlaPro: 2.77 ± 0.058	4.518AlaGln: 4.518 ± 0.073	2.809AlaArg: 2.809 ± 0.055	5.049AlaSer: 5.049 ± 0.088	5.235AlaThr: 5.235 ± 0.116	5.249AlaVal: 5.249 ± 0.081	0.91AlaTrp: 0.91 ± 0.033	2.983AlaTyr: 2.983 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.693CysAla: 0.693 ± 0.028	0.116CysCys: 0.116 ± 0.009	0.398CysAsp: 0.398 ± 0.02	0.46CysGlu: 0.46 ± 0.024	0.442CysPhe: 0.442 ± 0.019	0.731CysGly: 0.731 ± 0.032	0.182CysHis: 0.182 ± 0.013	0.581CysIle: 0.581 ± 0.022	0.456CysLys: 0.456 ± 0.021	0.792CysLeu: 0.792 ± 0.028	0.2CysMet: 0.2 ± 0.011	0.493CysAsn: 0.493 ± 0.022	0.358CysPro: 0.358 ± 0.022	0.374CysGln: 0.374 ± 0.02	0.325CysArg: 0.325 ± 0.018	0.633CysSer: 0.633 ± 0.032	0.522CysThr: 0.522 ± 0.028	0.604CysVal: 0.604 ± 0.025	0.115CysTrp: 0.115 ± 0.008	0.359CysTyr: 0.359 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.824AspAla: 3.824 ± 0.06	0.421AspCys: 0.421 ± 0.021	2.129AspAsp: 2.129 ± 0.055	3.065AspGlu: 3.065 ± 0.058	3.057AspPhe: 3.057 ± 0.056	3.6AspGly: 3.6 ± 0.143	0.614AspHis: 0.614 ± 0.024	3.541AspIle: 3.541 ± 0.063	3.579AspLys: 3.579 ± 0.068	4.65AspLeu: 4.65 ± 0.082	1.051AspMet: 1.051 ± 0.028	2.778AspAsn: 2.778 ± 0.076	1.38AspPro: 1.38 ± 0.037	1.251AspGln: 1.251 ± 0.033	1.808AspArg: 1.808 ± 0.046	3.129AspSer: 3.129 ± 0.074	3.068AspThr: 3.068 ± 0.069	3.099AspVal: 3.099 ± 0.05	0.731AspTrp: 0.731 ± 0.028	2.418AspTyr: 2.418 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.927GluAla: 4.927 ± 0.075	0.339GluCys: 0.339 ± 0.018	2.435GluAsp: 2.435 ± 0.046	3.602GluGlu: 3.602 ± 0.095	2.341GluPhe: 2.341 ± 0.052	3.373GluGly: 3.373 ± 0.055	1.127GluHis: 1.127 ± 0.028	4.311GluIle: 4.311 ± 0.077	4.944GluLys: 4.944 ± 0.096	5.625GluLeu: 5.625 ± 0.088	1.541GluMet: 1.541 ± 0.038	3.518GluAsn: 3.518 ± 0.058	1.66GluPro: 1.66 ± 0.036	3.056GluGln: 3.056 ± 0.061	2.507GluArg: 2.507 ± 0.052	2.943GluSer: 2.943 ± 0.052	3.073GluThr: 3.073 ± 0.059	3.523GluVal: 3.523 ± 0.064	0.627GluTrp: 0.627 ± 0.023	2.313GluTyr: 2.313 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	3.639PheAla: 3.639 ± 0.068	0.495PheCys: 0.495 ± 0.022	2.89PheAsp: 2.89 ± 0.051	2.951PheGlu: 2.951 ± 0.059	2.499PhePhe: 2.499 ± 0.049	3.481PheGly: 3.481 ± 0.056	0.733PheHis: 0.733 ± 0.025	2.83PheIle: 2.83 ± 0.057	2.74PheLys: 2.74 ± 0.051	4.303PheLeu: 4.303 ± 0.085	1.136PheMet: 1.136 ± 0.031	2.416PheAsn: 2.416 ± 0.075	1.574PhePro: 1.574 ± 0.036	1.522PheGln: 1.522 ± 0.034	1.91PheArg: 1.91 ± 0.037	3.58PheSer: 3.58 ± 0.065	3.008PheThr: 3.008 ± 0.068	3.644PheVal: 3.644 ± 0.053	0.712PheTrp: 0.712 ± 0.027	2.09PheTyr: 2.09 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.982GlyAla: 4.982 ± 0.102	0.719GlyCys: 0.719 ± 0.035	2.85GlyAsp: 2.85 ± 0.073	3.098GlyGlu: 3.098 ± 0.062	3.383GlyPhe: 3.383 ± 0.058	4.687GlyGly: 4.687 ± 0.086	1.229GlyHis: 1.229 ± 0.035	4.607GlyIle: 4.607 ± 0.091	4.466GlyLys: 4.466 ± 0.075	6.298GlyLeu: 6.298 ± 0.082	1.495GlyMet: 1.495 ± 0.042	3.535GlyAsn: 3.535 ± 0.081	0.851GlyPro: 0.851 ± 0.029	2.928GlyGln: 2.928 ± 0.056	2.476GlyArg: 2.476 ± 0.049	4.322GlySer: 4.322 ± 0.106	4.423GlyThr: 4.423 ± 0.13	4.666GlyVal: 4.666 ± 0.069	0.824GlyTrp: 0.824 ± 0.027	2.74GlyTyr: 2.74 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.173HisAla: 1.173 ± 0.036	0.211HisCys: 0.211 ± 0.013	0.836HisAsp: 0.836 ± 0.025	1.045HisGlu: 1.045 ± 0.033	1.199HisPhe: 1.199 ± 0.033	1.068HisGly: 1.068 ± 0.029	0.48HisHis: 0.48 ± 0.028	1.296HisIle: 1.296 ± 0.04	1.137HisLys: 1.137 ± 0.03	2.08HisLeu: 2.08 ± 0.044	0.226HisMet: 0.226 ± 0.015	0.944HisAsn: 0.944 ± 0.029	0.792HisPro: 0.792 ± 0.024	0.807HisGln: 0.807 ± 0.027	0.789HisArg: 0.789 ± 0.028	1.096HisSer: 1.096 ± 0.03	1.148HisThr: 1.148 ± 0.032	0.916HisVal: 0.916 ± 0.028	0.296HisTrp: 0.296 ± 0.015	0.929HisTyr: 0.929 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.61IleAla: 5.61 ± 0.079	0.687IleCys: 0.687 ± 0.027	3.749IleAsp: 3.749 ± 0.063	4.163IleGlu: 4.163 ± 0.076	3.109IlePhe: 3.109 ± 0.056	4.407IleGly: 4.407 ± 0.066	1.091IleHis: 1.091 ± 0.032	4.245IleIle: 4.245 ± 0.077	4.191IleLys: 4.191 ± 0.064	5.752IleLeu: 5.752 ± 0.079	1.241IleMet: 1.241 ± 0.037	3.568IleAsn: 3.568 ± 0.055	2.53IlePro: 2.53 ± 0.048	2.418IleGln: 2.418 ± 0.046	2.748IleArg: 2.748 ± 0.054	4.912IleSer: 4.912 ± 0.081	4.42IleThr: 4.42 ± 0.072	4.386IleVal: 4.386 ± 0.064	0.71IleTrp: 0.71 ± 0.029	2.502IleTyr: 2.502 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	5.346LysAla: 5.346 ± 0.087	0.351LysCys: 0.351 ± 0.017	3.301LysAsp: 3.301 ± 0.068	4.213LysGlu: 4.213 ± 0.08	2.362LysPhe: 2.362 ± 0.049	3.81LysGly: 3.81 ± 0.064	1.304LysHis: 1.304 ± 0.034	4.911LysIle: 4.911 ± 0.077	4.872LysLys: 4.872 ± 0.085	5.648LysLeu: 5.648 ± 0.088	1.744LysMet: 1.744 ± 0.041	3.911LysAsn: 3.911 ± 0.068	2.562LysPro: 2.562 ± 0.049	2.888LysGln: 2.888 ± 0.055	2.527LysArg: 2.527 ± 0.055	3.555LysSer: 3.555 ± 0.067	3.849LysThr: 3.849 ± 0.065	4.178LysVal: 4.178 ± 0.07	0.632LysTrp: 0.632 ± 0.025	2.651LysTyr: 2.651 ± 0.049	0.0LysXaa: 0.0 ± 0.0
Leu	8.008LeuAla: 8.008 ± 0.101	0.936LeuCys: 0.936 ± 0.034	4.675LeuAsp: 4.675 ± 0.083	5.302LeuGlu: 5.302 ± 0.093	4.423LeuPhe: 4.423 ± 0.077	6.093LeuGly: 6.093 ± 0.08	1.968LeuHis: 1.968 ± 0.044	5.473LeuIle: 5.473 ± 0.081	5.96LeuLys: 5.96 ± 0.094	9.705LeuLeu: 9.705 ± 0.137	2.154LeuMet: 2.154 ± 0.047	4.87LeuAsn: 4.87 ± 0.069	4.43LeuPro: 4.43 ± 0.069	4.38LeuGln: 4.38 ± 0.067	4.268LeuArg: 4.268 ± 0.065	6.922LeuSer: 6.922 ± 0.084	5.416LeuThr: 5.416 ± 0.076	5.812LeuVal: 5.812 ± 0.08	1.076LeuTrp: 1.076 ± 0.035	3.531LeuTyr: 3.531 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.019MetAla: 2.019 ± 0.043	0.153MetCys: 0.153 ± 0.012	0.887MetAsp: 0.887 ± 0.026	1.189MetGlu: 1.189 ± 0.04	0.941MetPhe: 0.941 ± 0.037	1.464MetGly: 1.464 ± 0.034	0.42MetHis: 0.42 ± 0.019	1.293MetIle: 1.293 ± 0.037	1.674MetLys: 1.674 ± 0.035	2.306MetLeu: 2.306 ± 0.049	0.572MetMet: 0.572 ± 0.024	1.12MetAsn: 1.12 ± 0.029	1.118MetPro: 1.118 ± 0.031	1.115MetGln: 1.115 ± 0.03	1.108MetArg: 1.108 ± 0.031	1.436MetSer: 1.436 ± 0.036	1.16MetThr: 1.16 ± 0.032	1.303MetVal: 1.303 ± 0.038	0.173MetTrp: 0.173 ± 0.013	0.758MetTyr: 0.758 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	4.289AsnAla: 4.289 ± 0.067	0.462AsnCys: 0.462 ± 0.026	2.524AsnAsp: 2.524 ± 0.073	2.763AsnGlu: 2.763 ± 0.05	2.577AsnPhe: 2.577 ± 0.053	3.832AsnGly: 3.832 ± 0.094	0.868AsnHis: 0.868 ± 0.026	4.279AsnIle: 4.279 ± 0.065	3.423AsnLys: 3.423 ± 0.058	4.963AsnLeu: 4.963 ± 0.07	1.147AsnMet: 1.147 ± 0.038	3.467AsnAsn: 3.467 ± 0.098	2.831AsnPro: 2.831 ± 0.061	2.285AsnGln: 2.285 ± 0.052	2.004AsnArg: 2.004 ± 0.044	3.318AsnSer: 3.318 ± 0.071	3.779AsnThr: 3.779 ± 0.086	3.457AsnVal: 3.457 ± 0.068	0.665AsnTrp: 0.665 ± 0.029	2.437AsnTyr: 2.437 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	3.304ProAla: 3.304 ± 0.06	0.287ProCys: 0.287 ± 0.018	2.313ProAsp: 2.313 ± 0.051	2.68ProGlu: 2.68 ± 0.055	1.751ProPhe: 1.751 ± 0.037	1.031ProGly: 1.031 ± 0.037	0.774ProHis: 0.774 ± 0.027	2.376ProIle: 2.376 ± 0.045	2.158ProLys: 2.158 ± 0.047	3.485ProLeu: 3.485 ± 0.05	0.793ProMet: 0.793 ± 0.026	2.437ProAsn: 2.437 ± 0.05	1.228ProPro: 1.228 ± 0.045	1.697ProGln: 1.697 ± 0.042	1.238ProArg: 1.238 ± 0.032	2.527ProSer: 2.527 ± 0.063	2.68ProThr: 2.68 ± 0.066	2.78ProVal: 2.78 ± 0.057	0.273ProTrp: 0.273 ± 0.018	1.445ProTyr: 1.445 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	3.676GlnAla: 3.676 ± 0.072	0.273GlnCys: 0.273 ± 0.016	1.789GlnAsp: 1.789 ± 0.034	2.483GlnGlu: 2.483 ± 0.052	1.722GlnPhe: 1.722 ± 0.038	2.077GlnGly: 2.077 ± 0.041	0.946GlnHis: 0.946 ± 0.03	3.091GlnIle: 3.091 ± 0.056	3.465GlnLys: 3.465 ± 0.059	4.261GlnLeu: 4.261 ± 0.067	1.19GlnMet: 1.19 ± 0.03	2.714GlnAsn: 2.714 ± 0.058	1.633GlnPro: 1.633 ± 0.043	2.585GlnGln: 2.585 ± 0.058	1.807GlnArg: 1.807 ± 0.039	2.295GlnSer: 2.295 ± 0.045	3.035GlnThr: 3.035 ± 0.052	2.531GlnVal: 2.531 ± 0.049	0.626GlnTrp: 0.626 ± 0.024	1.747GlnTyr: 1.747 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	2.637ArgAla: 2.637 ± 0.053	0.28ArgCys: 0.28 ± 0.015	1.928ArgAsp: 1.928 ± 0.045	2.515ArgGlu: 2.515 ± 0.053	2.054ArgPhe: 2.054 ± 0.045	2.063ArgGly: 2.063 ± 0.051	0.823ArgHis: 0.823 ± 0.021	2.914ArgIle: 2.914 ± 0.053	2.876ArgLys: 2.876 ± 0.054	4.032ArgLeu: 4.032 ± 0.06	1.037ArgMet: 1.037 ± 0.034	2.244ArgAsn: 2.244 ± 0.045	1.456ArgPro: 1.456 ± 0.038	1.885ArgGln: 1.885 ± 0.042	1.917ArgArg: 1.917 ± 0.051	1.905ArgSer: 1.905 ± 0.05	2.213ArgThr: 2.213 ± 0.047	2.647ArgVal: 2.647 ± 0.047	0.561ArgTrp: 0.561 ± 0.023	1.86ArgTyr: 1.86 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	5.119SerAla: 5.119 ± 0.081	0.624SerCys: 0.624 ± 0.027	3.19SerAsp: 3.19 ± 0.069	3.404SerGlu: 3.404 ± 0.055	3.446SerPhe: 3.446 ± 0.062	4.828SerGly: 4.828 ± 0.119	1.134SerHis: 1.134 ± 0.032	4.175SerIle: 4.175 ± 0.072	3.519SerLys: 3.519 ± 0.062	6.281SerLeu: 6.281 ± 0.095	1.275SerMet: 1.275 ± 0.031	3.342SerAsn: 3.342 ± 0.074	2.66SerPro: 2.66 ± 0.058	2.491SerGln: 2.491 ± 0.052	2.336SerArg: 2.336 ± 0.047	4.326SerSer: 4.326 ± 0.091	3.758SerThr: 3.758 ± 0.102	4.934SerVal: 4.934 ± 0.088	0.7SerTrp: 0.7 ± 0.027	2.743SerTyr: 2.743 ± 0.061	0.0SerXaa: 0.0 ± 0.0
Thr	5.511ThrAla: 5.511 ± 0.116	0.448ThrCys: 0.448 ± 0.021	3.522ThrAsp: 3.522 ± 0.071	3.323ThrGlu: 3.323 ± 0.052	2.977ThrPhe: 2.977 ± 0.057	4.54ThrGly: 4.54 ± 0.114	1.162ThrHis: 1.162 ± 0.032	3.924ThrIle: 3.924 ± 0.066	3.092ThrLys: 3.092 ± 0.055	6.231ThrLeu: 6.231 ± 0.1	1.057ThrMet: 1.057 ± 0.036	3.269ThrAsn: 3.269 ± 0.081	3.05ThrPro: 3.05 ± 0.061	2.646ThrGln: 2.646 ± 0.052	2.034ThrArg: 2.034 ± 0.04	3.916ThrSer: 3.916 ± 0.116	3.924ThrThr: 3.924 ± 0.114	4.284ThrVal: 4.284 ± 0.122	0.692ThrTrp: 0.692 ± 0.032	2.364ThrTyr: 2.364 ± 0.068	0.0ThrXaa: 0.0 ± 0.0
Val	5.953ValAla: 5.953 ± 0.092	0.746ValCys: 0.746 ± 0.029	2.609ValAsp: 2.609 ± 0.045	3.635ValGlu: 3.635 ± 0.062	3.242ValPhe: 3.242 ± 0.06	4.534ValGly: 4.534 ± 0.068	1.099ValHis: 1.099 ± 0.034	4.153ValIle: 4.153 ± 0.063	3.747ValLys: 3.747 ± 0.062	6.307ValLeu: 6.307 ± 0.08	1.472ValMet: 1.472 ± 0.043	3.29ValAsn: 3.29 ± 0.067	2.536ValPro: 2.536 ± 0.046	2.556ValGln: 2.556 ± 0.051	2.903ValArg: 2.903 ± 0.05	4.962ValSer: 4.962 ± 0.091	3.97ValThr: 3.97 ± 0.095	4.865ValVal: 4.865 ± 0.075	0.816ValTrp: 0.816 ± 0.026	2.632ValTyr: 2.632 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.953TrpAla: 0.953 ± 0.031	0.112TrpCys: 0.112 ± 0.01	0.57TrpAsp: 0.57 ± 0.022	0.627TrpGlu: 0.627 ± 0.025	0.596TrpPhe: 0.596 ± 0.023	0.805TrpGly: 0.805 ± 0.032	0.265TrpHis: 0.265 ± 0.015	0.694TrpIle: 0.694 ± 0.025	0.681TrpLys: 0.681 ± 0.027	1.352TrpLeu: 1.352 ± 0.035	0.173TrpMet: 0.173 ± 0.012	0.661TrpAsn: 0.661 ± 0.031	0.359TrpPro: 0.359 ± 0.017	0.739TrpGln: 0.739 ± 0.026	0.553TrpArg: 0.553 ± 0.023	0.696TrpSer: 0.696 ± 0.029	0.681TrpThr: 0.681 ± 0.029	0.79TrpVal: 0.79 ± 0.031	0.174TrpTrp: 0.174 ± 0.013	0.458TrpTyr: 0.458 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.959TyrAla: 2.959 ± 0.057	0.388TyrCys: 0.388 ± 0.018	2.006TyrAsp: 2.006 ± 0.043	2.311TyrGlu: 2.311 ± 0.048	2.321TyrPhe: 2.321 ± 0.046	2.525TyrGly: 2.525 ± 0.053	0.748TyrHis: 0.748 ± 0.024	2.387TyrIle: 2.387 ± 0.049	2.658TyrLys: 2.658 ± 0.052	3.975TyrLeu: 3.975 ± 0.055	0.829TyrMet: 0.829 ± 0.028	2.44TyrAsn: 2.44 ± 0.056	1.476TyrPro: 1.476 ± 0.035	1.807TyrGln: 1.807 ± 0.041	1.779TyrArg: 1.779 ± 0.041	2.763TyrSer: 2.763 ± 0.056	2.604TyrThr: 2.604 ± 0.066	2.36TyrVal: 2.36 ± 0.046	0.625TyrTrp: 0.625 ± 0.024	2.015TyrTyr: 2.015 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3664 proteins (1257853 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---