Geodermatophilus sabuli
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5204 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A285EFZ0|A0A285EFZ0_9ACTN Acyl-CoA thioesterase OS=Geodermatophilus sabuli OX=1564158 GN=SAMN06893097_105292 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.3 LSKK5 pKa = 9.72 RR6 pKa = 11.84 TAALIATGLSGVMVLSACGGGDD28 pKa = 4.1 DD29 pKa = 4.83 EE30 pKa = 5.76 GSSSADD36 pKa = 2.87 GGSFSIYY43 pKa = 9.67 IGEE46 pKa = 4.3 PEE48 pKa = 4.27 NPLMPGNTNEE58 pKa = 4.29 TEE60 pKa = 4.22 GGQVLDD66 pKa = 3.86 SLFTGLVQYY75 pKa = 10.87 DD76 pKa = 3.94 PEE78 pKa = 4.34 TNEE81 pKa = 3.74 AAFTGVAEE89 pKa = 4.77 SIEE92 pKa = 4.73 SDD94 pKa = 4.19 DD95 pKa = 3.61 QTTWTVKK102 pKa = 10.86 LNDD105 pKa = 2.97 GWTFHH110 pKa = 7.46 DD111 pKa = 5.08 GSPVNAQSFVDD122 pKa = 3.58 AWNYY126 pKa = 7.22 TAYY129 pKa = 10.76 SPNAQGNSYY138 pKa = 10.34 FFANIVGYY146 pKa = 10.98 GDD148 pKa = 3.7 LQAPEE153 pKa = 4.64 AGGDD157 pKa = 3.71 PVATEE162 pKa = 3.88 MSGLRR167 pKa = 11.84 VVDD170 pKa = 4.26 DD171 pKa = 3.66 LTFEE175 pKa = 4.42 VTLSSPYY182 pKa = 10.13 AQWPTTVGYY191 pKa = 7.89 TAFFPLPPAFFDD203 pKa = 4.09 DD204 pKa = 4.05 PAAFGEE210 pKa = 4.21 QPIGNGPFQAVEE222 pKa = 4.13 PFVPGQGVTLTRR234 pKa = 11.84 YY235 pKa = 9.96 EE236 pKa = 4.45 DD237 pKa = 3.68 FAGDD241 pKa = 3.71 EE242 pKa = 4.1 PAKK245 pKa = 10.71 AEE247 pKa = 4.12 SVEE250 pKa = 3.61 YY251 pKa = 9.76 RR252 pKa = 11.84 VYY254 pKa = 11.0 VEE256 pKa = 4.47 QDD258 pKa = 2.75 TAYY261 pKa = 9.82 TDD263 pKa = 3.77 LQGGSLDD270 pKa = 4.88 IMDD273 pKa = 5.5 TLPPDD278 pKa = 5.17 AIASAEE284 pKa = 4.19 SEE286 pKa = 3.99 FGDD289 pKa = 4.44 RR290 pKa = 11.84 YY291 pKa = 10.57 IEE293 pKa = 4.07 TAQGDD298 pKa = 3.84 ITSLGFPTYY307 pKa = 9.52 DD308 pKa = 4.34 QRR310 pKa = 11.84 FADD313 pKa = 3.84 PNVRR317 pKa = 11.84 KK318 pKa = 9.59 AFSMAIDD325 pKa = 3.7 RR326 pKa = 11.84 QAISDD331 pKa = 4.26 AIFNGTRR338 pKa = 11.84 TPATSFISPVVPGYY352 pKa = 10.64 RR353 pKa = 11.84 EE354 pKa = 4.25 GACEE358 pKa = 3.62 ACEE361 pKa = 4.41 LNVEE365 pKa = 4.42 EE366 pKa = 5.48 ANSLLDD372 pKa = 3.3 AAGFDD377 pKa = 3.54 RR378 pKa = 11.84 SQPVDD383 pKa = 2.84 LWFNAGAGHH392 pKa = 6.64 DD393 pKa = 3.49 AWMEE397 pKa = 3.82 AVGNQLRR404 pKa = 11.84 EE405 pKa = 4.15 NLGVEE410 pKa = 3.96 YY411 pKa = 10.03 QLRR414 pKa = 11.84 GDD416 pKa = 4.32 LPQAEE421 pKa = 4.58 FLPLKK426 pKa = 9.57 DD427 pKa = 3.92 AKK429 pKa = 11.18 GMTGPFRR436 pKa = 11.84 DD437 pKa = 4.18 AWIMDD442 pKa = 3.57 YY443 pKa = 10.75 PVAEE447 pKa = 4.34 NFLGPLYY454 pKa = 10.76 SSVALPPAGSNYY466 pKa = 9.09 TFYY469 pKa = 11.36 SNPQFDD475 pKa = 4.07 QLLQQGNAAASDD487 pKa = 4.06 DD488 pKa = 4.19 EE489 pKa = 5.4 AVAAYY494 pKa = 9.26 QAAEE498 pKa = 4.27 DD499 pKa = 4.12 VLIADD504 pKa = 5.16 LPSAPLFYY512 pKa = 10.72 RR513 pKa = 11.84 LNQGAHH519 pKa = 5.92 SEE521 pKa = 4.17 NVDD524 pKa = 3.37 NVIIDD529 pKa = 3.34 AFGRR533 pKa = 11.84 IDD535 pKa = 3.23 AAAVEE540 pKa = 4.93 VVSS543 pKa = 4.44
Molecular weight: 58.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.732
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.872
Patrickios 1.38
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|A0A285E903|A0A285E903_9ACTN Drug resistance transporter EmrB/QacA subfamily OS=Geodermatophilus sabuli OX=1564158 GN=SAMN06893097_102205 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.51 LLKK22 pKa = 8.15 KK23 pKa = 9.25 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.81 KK32 pKa = 9.63
Molecular weight: 4.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5204
0
5204
1639597
29
2138
315.1
33.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.326 ± 0.054
0.718 ± 0.009
6.187 ± 0.024
5.586 ± 0.029
2.611 ± 0.018
9.659 ± 0.03
2.076 ± 0.015
2.794 ± 0.022
1.265 ± 0.017
10.643 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.645 ± 0.013
1.433 ± 0.016
6.322 ± 0.031
2.686 ± 0.019
8.268 ± 0.037
4.758 ± 0.022
5.971 ± 0.025
9.81 ± 0.03
1.476 ± 0.014
1.766 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here