Oryzihumus leptocrescens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Oryzihumus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4026 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A542Z9B9|A0A542Z9B9_9MICO Alpha/beta hydrolase family protein OS=Oryzihumus leptocrescens OX=297536 GN=FB474_3707 PE=4 SV=1
MM1 pKa = 7.23SQTTTITVTGMTCGHH16 pKa = 7.33CAQAVTGEE24 pKa = 4.36LSAVPGVTDD33 pKa = 3.23VAVDD37 pKa = 3.89LRR39 pKa = 11.84AGDD42 pKa = 4.38DD43 pKa = 3.77SPVTITSDD51 pKa = 3.2ATLDD55 pKa = 3.75PAAVRR60 pKa = 11.84AAVAEE65 pKa = 4.12AGYY68 pKa = 10.98AVATT72 pKa = 4.23

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A542ZN55|A0A542ZN55_9MICO Thiol-disulfide isomerase/thioredoxin OS=Oryzihumus leptocrescens OX=297536 GN=FB474_3145 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4026

0

4026

1271052

32

2031

315.7

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.705 ± 0.063

0.732 ± 0.012

5.89 ± 0.034

5.337 ± 0.045

2.661 ± 0.021

9.389 ± 0.033

2.36 ± 0.021

2.985 ± 0.026

1.842 ± 0.03

10.379 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.015

1.677 ± 0.021

5.906 ± 0.028

2.953 ± 0.021

7.827 ± 0.043

5.29 ± 0.03

6.187 ± 0.036

9.576 ± 0.038

1.627 ± 0.016

1.867 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski