Capybara microvirus Cap3_SP_481
Average proteome isoelectric point is 5.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8R9|A0A4P8W8R9_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_481 OX=2585469 PE=4 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.28 FYY4 pKa = 10.04 TAYY7 pKa = 10.32 NRR9 pKa = 11.84 PASEE13 pKa = 3.91 PTEE16 pKa = 4.36 IGSSIYY22 pKa = 9.94 PIYY25 pKa = 10.14 EE26 pKa = 3.4 PRR28 pKa = 11.84 IDD30 pKa = 3.58 KK31 pKa = 9.23 KK32 pKa = 9.16 TGRR35 pKa = 11.84 KK36 pKa = 8.86 ILIKK40 pKa = 10.7 VGEE43 pKa = 4.02 EE44 pKa = 3.58 DD45 pKa = 4.02 LYY47 pKa = 11.68 EE48 pKa = 4.82 KK49 pKa = 10.33 IQEE52 pKa = 4.29 SLEE55 pKa = 3.97 GTKK58 pKa = 9.82 IEE60 pKa = 4.42 NIVRR64 pKa = 11.84 RR65 pKa = 11.84 VTLGDD70 pKa = 3.43 PTALEE75 pKa = 3.96 QRR77 pKa = 11.84 IGQYY81 pKa = 11.02 LDD83 pKa = 3.37 LTEE86 pKa = 4.24 MPEE89 pKa = 4.26 NLMAAQNFILKK100 pKa = 10.53 AEE102 pKa = 4.31 AEE104 pKa = 4.27 FEE106 pKa = 4.17 KK107 pKa = 11.04 LPLEE111 pKa = 3.86 IRR113 pKa = 11.84 KK114 pKa = 8.81 EE115 pKa = 4.11 FNYY118 pKa = 9.43 STEE121 pKa = 4.46 EE122 pKa = 3.92 YY123 pKa = 9.65 VSAYY127 pKa = 10.2 GSEE130 pKa = 3.72 EE131 pKa = 3.55 WANRR135 pKa = 11.84 VGLTKK140 pKa = 10.39 VQEE143 pKa = 4.47 EE144 pKa = 4.26 IKK146 pKa = 10.49 EE147 pKa = 4.01 AATGVTADD155 pKa = 4.02 DD156 pKa = 4.01 QRR158 pKa = 11.84 NEE160 pKa = 3.71
Molecular weight: 18.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.787
IPC2_protein 4.774
IPC_protein 4.647
Toseland 4.52
ProMoST 4.66
Dawson 4.558
Bjellqvist 4.736
Wikipedia 4.368
Rodwell 4.482
Grimsley 4.431
Solomon 4.558
Lehninger 4.507
Nozaki 4.66
DTASelect 4.724
Thurlkill 4.495
EMBOSS 4.393
Sillero 4.749
Patrickios 4.037
IPC_peptide 4.571
IPC2_peptide 4.749
IPC2.peptide.svr19 4.692
Protein with the highest isoelectric point:
>tr|A0A4P8W6B1|A0A4P8W6B1_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_481 OX=2585469 PE=4 SV=1
MM1 pKa = 7.55 ACSSPLTGIIDD12 pKa = 3.73 QKK14 pKa = 11.47 GKK16 pKa = 8.66 ITFEE20 pKa = 4.2 SIQLKK25 pKa = 10.46 DD26 pKa = 3.36 GEE28 pKa = 4.72 SIKK31 pKa = 10.28 EE32 pKa = 3.73 RR33 pKa = 11.84 KK34 pKa = 8.67 QRR36 pKa = 11.84 LEE38 pKa = 3.49 NSGYY42 pKa = 9.02 ISKK45 pKa = 10.36 IIQIPCRR52 pKa = 11.84 RR53 pKa = 11.84 CMGCRR58 pKa = 11.84 LDD60 pKa = 4.9 YY61 pKa = 11.08 AKK63 pKa = 10.65 EE64 pKa = 3.52 WANRR68 pKa = 11.84 LTLEE72 pKa = 4.53 TKK74 pKa = 9.91 TSEE77 pKa = 3.75 NNYY80 pKa = 10.09 FITLTYY86 pKa = 10.55 DD87 pKa = 3.42 DD88 pKa = 4.41 NNIPIRR94 pKa = 11.84 EE95 pKa = 4.11 NKK97 pKa = 10.51 GEE99 pKa = 4.29 FISFPLNKK107 pKa = 9.4 KK108 pKa = 9.56 DD109 pKa = 5.36 AQDD112 pKa = 2.8 FWKK115 pKa = 10.18 RR116 pKa = 11.84 VRR118 pKa = 11.84 AKK120 pKa = 10.69 YY121 pKa = 9.81 PEE123 pKa = 3.76 PHH125 pKa = 6.31 IKK127 pKa = 10.66 YY128 pKa = 9.61 FMCGEE133 pKa = 4.17 YY134 pKa = 10.86 GEE136 pKa = 4.53 EE137 pKa = 3.89 TGRR140 pKa = 11.84 PHH142 pKa = 5.31 YY143 pKa = 10.24 HH144 pKa = 6.75 AIIYY148 pKa = 7.29 NAPWLNDD155 pKa = 3.12 LKK157 pKa = 11.04 YY158 pKa = 10.92 YY159 pKa = 10.54 KK160 pKa = 10.73 NNEE163 pKa = 3.66 FGDD166 pKa = 4.03 ALFHH170 pKa = 7.68 SEE172 pKa = 4.82 ILNKK176 pKa = 10.31 LWGKK180 pKa = 11.13 GDD182 pKa = 3.6 TTVGDD187 pKa = 4.0 VTWNSSSYY195 pKa = 7.73 VARR198 pKa = 11.84 YY199 pKa = 6.88 ITKK202 pKa = 9.77 KK203 pKa = 10.58 QYY205 pKa = 9.87 GDD207 pKa = 3.29 KK208 pKa = 10.82 AAEE211 pKa = 4.21 HH212 pKa = 6.39 YY213 pKa = 9.13 EE214 pKa = 3.98 NLGLEE219 pKa = 4.38 PEE221 pKa = 4.41 FVTMSLKK228 pKa = 10.72 PMIGQEE234 pKa = 4.14 YY235 pKa = 9.04 YY236 pKa = 10.39 DD237 pKa = 3.35 QHH239 pKa = 8.46 KK240 pKa = 9.26 EE241 pKa = 3.69 QIYY244 pKa = 9.98 KK245 pKa = 10.3 NDD247 pKa = 4.11 YY248 pKa = 10.18 IWINCKK254 pKa = 10.81 GEE256 pKa = 4.21 TIKK259 pKa = 10.64 IKK261 pKa = 10.31 PPRR264 pKa = 11.84 IYY266 pKa = 10.4 DD267 pKa = 3.7 LKK269 pKa = 11.32 YY270 pKa = 10.16 EE271 pKa = 4.12 IEE273 pKa = 4.1 NPEE276 pKa = 3.72 RR277 pKa = 11.84 MKK279 pKa = 10.5 EE280 pKa = 3.81 IKK282 pKa = 9.89 LQRR285 pKa = 11.84 QLRR288 pKa = 11.84 QEE290 pKa = 3.84 AATAEE295 pKa = 4.43 IINNTGYY302 pKa = 10.3 IDD304 pKa = 3.98 GAQMQRR310 pKa = 11.84 EE311 pKa = 4.36 IKK313 pKa = 10.49 ARR315 pKa = 11.84 ALDD318 pKa = 3.59 ARR320 pKa = 11.84 TANLKK325 pKa = 8.91 RR326 pKa = 11.84 TII328 pKa = 3.82
Molecular weight: 38.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.489
IPC2_protein 7.834
IPC_protein 7.717
Toseland 7.585
ProMoST 8.229
Dawson 8.463
Bjellqvist 8.639
Wikipedia 8.448
Rodwell 8.507
Grimsley 7.556
Solomon 8.58
Lehninger 8.565
Nozaki 8.726
DTASelect 8.448
Thurlkill 8.521
EMBOSS 8.653
Sillero 8.77
Patrickios 4.215
IPC_peptide 8.565
IPC2_peptide 7.424
IPC2.peptide.svr19 7.742
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1379
78
580
275.8
31.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.759 ± 1.103
0.725 ± 0.366
4.496 ± 0.34
8.484 ± 1.682
3.481 ± 1.044
7.179 ± 0.916
1.378 ± 0.385
7.179 ± 1.261
6.599 ± 1.253
5.729 ± 0.418
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.191 ± 0.471
6.526 ± 1.147
4.206 ± 0.923
4.641 ± 0.376
4.206 ± 0.71
6.164 ± 1.601
7.179 ± 0.653
3.191 ± 0.649
2.03 ± 0.412
5.656 ± 0.455
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here