bacterium LRH843

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P1B1A5|A0A6P1B1A5_9BACT Uncharacterized protein (Fragment) OS=bacterium LRH843 OX=2681972 GN=GN156_20715 PE=4 SV=1
LL1 pKa = 7.62ADD3 pKa = 4.57RR4 pKa = 11.84LSIDD8 pKa = 3.29AQGTQDD14 pKa = 5.41HH15 pKa = 6.75IFLMYY20 pKa = 10.84DD21 pKa = 3.04NEE23 pKa = 4.43IYY25 pKa = 10.76LDD27 pKa = 3.74RR28 pKa = 11.84FNSLEE33 pKa = 4.01TAALFVDD40 pKa = 4.07LGVYY44 pKa = 9.94DD45 pKa = 4.2AAFLSLRR52 pKa = 11.84DD53 pKa = 4.01DD54 pKa = 3.58RR55 pKa = 11.84VAAYY59 pKa = 9.53LKK61 pKa = 8.71QQGYY65 pKa = 9.52VSEE68 pKa = 4.19EE69 pKa = 4.02QIDD72 pKa = 4.07DD73 pKa = 4.14LQCALNPLFCTDD85 pKa = 2.85VV86 pKa = 3.13

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P1AZY4|A0A6P1AZY4_9BACT Uncharacterized protein (Fragment) OS=bacterium LRH843 OX=2681972 GN=GN156_28935 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.46KK3 pKa = 8.43PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.15AGRR29 pKa = 11.84NVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7593

0

7593

1363251

18

1552

179.5

20.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.654 ± 0.041

0.795 ± 0.01

5.15 ± 0.03

7.364 ± 0.04

4.336 ± 0.03

7.031 ± 0.034

2.176 ± 0.017

7.563 ± 0.033

6.328 ± 0.031

9.792 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.016

4.181 ± 0.024

3.731 ± 0.017

3.912 ± 0.025

4.404 ± 0.028

6.001 ± 0.023

5.447 ± 0.022

7.114 ± 0.029

1.009 ± 0.011

3.271 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski