Synechococcus phage S-RSM4
Average proteome isoelectric point is 5.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7BUY8|C7BUY8_9CAUD Photosystem II OS=Synechococcus phage S-RSM4 OX=555387 GN=psbD PE=3 SV=1
MM1 pKa = 7.2 AQATVLYY8 pKa = 9.56 FSATVSFIFLCVGVIAGWTANEE30 pKa = 4.01 KK31 pKa = 10.33 LHH33 pKa = 6.08 EE34 pKa = 4.23 FMYY37 pKa = 10.76 GKK39 pKa = 10.1 AQEE42 pKa = 4.4 EE43 pKa = 4.51 NIHH46 pKa = 6.71 PEE48 pKa = 3.91 MLDD51 pKa = 5.51 GEE53 pKa = 4.67 GQWINEE59 pKa = 4.03 EE60 pKa = 4.08 LLSVRR65 pKa = 11.84 FVDD68 pKa = 5.73 EE69 pKa = 6.2 DD70 pKa = 4.53 DD71 pKa = 5.82 DD72 pKa = 7.0 DD73 pKa = 6.54 YY74 pKa = 12.18 DD75 pKa = 4.35 DD76 pKa = 4.26
Molecular weight: 8.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.834
IPC_protein 3.757
Toseland 3.567
ProMoST 3.846
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.872
Patrickios 1.863
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|C7BV11|C7BV11_9CAUD Hypothetical cyanophage protein OS=Synechococcus phage S-RSM4 OX=555387 GN=SRSM4_043 PE=4 SV=1
MM1 pKa = 7.62 GFTTPRR7 pKa = 11.84 VSYY10 pKa = 9.43 FLKK13 pKa = 10.94 VSGADD18 pKa = 3.27 GNTPAWFLASSQRR31 pKa = 11.84 ASGGSKK37 pKa = 10.27
Molecular weight: 3.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.252
IPC2_protein 9.721
IPC_protein 10.175
Toseland 10.511
ProMoST 10.335
Dawson 10.628
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.067
Grimsley 10.687
Solomon 10.716
Lehninger 10.701
Nozaki 10.452
DTASelect 10.277
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.54
Patrickios 11.023
IPC_peptide 10.716
IPC2_peptide 8.931
IPC2.peptide.svr19 8.638
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
237
0
237
60981
37
4482
257.3
28.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.884 ± 0.22
0.841 ± 0.074
6.878 ± 0.137
6.248 ± 0.231
4.408 ± 0.104
8.142 ± 0.405
1.469 ± 0.112
6.235 ± 0.15
5.426 ± 0.297
7.071 ± 0.145
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.871 ± 0.174
5.912 ± 0.151
4.023 ± 0.097
3.68 ± 0.111
4.101 ± 0.132
6.956 ± 0.238
7.422 ± 0.316
6.753 ± 0.178
1.194 ± 0.084
4.487 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here