Amino acid dipepetide frequency for Synechococcus phage S-RSM4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.396AlaAla: 6.396 ± 0.512
0.607AlaCys: 0.607 ± 0.111
4.378AlaAsp: 4.378 ± 0.292
4.051AlaGlu: 4.051 ± 0.269
2.837AlaPhe: 2.837 ± 0.259
6.379AlaGly: 6.379 ± 0.481
0.918AlaHis: 0.918 ± 0.119
4.198AlaIle: 4.198 ± 0.315
3.345AlaLys: 3.345 ± 0.29
4.87AlaLeu: 4.87 ± 0.309
1.279AlaMet: 1.279 ± 0.165
4.182AlaAsn: 4.182 ± 0.357
2.935AlaPro: 2.935 ± 0.264
2.279AlaGln: 2.279 ± 0.241
2.345AlaArg: 2.345 ± 0.208
5.166AlaSer: 5.166 ± 0.406
5.346AlaThr: 5.346 ± 0.358
4.001AlaVal: 4.001 ± 0.244
0.64AlaTrp: 0.64 ± 0.109
2.689AlaTyr: 2.689 ± 0.217
0.0AlaXaa: 0.0 ± 0.0
Cys
0.705CysAla: 0.705 ± 0.113
0.033CysCys: 0.033 ± 0.023
0.705CysAsp: 0.705 ± 0.108
0.59CysGlu: 0.59 ± 0.086
0.344CysPhe: 0.344 ± 0.074
0.59CysGly: 0.59 ± 0.119
0.197CysHis: 0.197 ± 0.062
0.574CysIle: 0.574 ± 0.121
0.672CysLys: 0.672 ± 0.12
0.492CysLeu: 0.492 ± 0.088
0.164CysMet: 0.164 ± 0.054
0.377CysAsn: 0.377 ± 0.071
0.312CysPro: 0.312 ± 0.065
0.394CysGln: 0.394 ± 0.081
0.344CysArg: 0.344 ± 0.08
0.476CysSer: 0.476 ± 0.095
0.312CysThr: 0.312 ± 0.071
0.59CysVal: 0.59 ± 0.101
0.131CysTrp: 0.131 ± 0.046
0.41CysTyr: 0.41 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
5.018AspAla: 5.018 ± 0.505
0.541AspCys: 0.541 ± 0.093
4.887AspAsp: 4.887 ± 0.34
4.001AspGlu: 4.001 ± 0.298
3.132AspPhe: 3.132 ± 0.238
6.1AspGly: 6.1 ± 0.378
1.115AspHis: 1.115 ± 0.16
4.329AspIle: 4.329 ± 0.322
3.427AspLys: 3.427 ± 0.281
5.002AspLeu: 5.002 ± 0.372
1.296AspMet: 1.296 ± 0.202
3.755AspAsn: 3.755 ± 0.285
3.214AspPro: 3.214 ± 0.342
2.181AspGln: 2.181 ± 0.147
2.837AspArg: 2.837 ± 0.259
4.1AspSer: 4.1 ± 0.315
4.805AspThr: 4.805 ± 0.34
4.411AspVal: 4.411 ± 0.276
1.132AspTrp: 1.132 ± 0.155
3.493AspTyr: 3.493 ± 0.3
0.0AspXaa: 0.0 ± 0.0
Glu
3.755GluAla: 3.755 ± 0.219
0.64GluCys: 0.64 ± 0.105
4.001GluAsp: 4.001 ± 0.33
5.362GluGlu: 5.362 ± 0.476
2.821GluPhe: 2.821 ± 0.243
4.133GluGly: 4.133 ± 0.306
1.017GluHis: 1.017 ± 0.15
4.133GluIle: 4.133 ± 0.279
4.116GluLys: 4.116 ± 0.434
4.641GluLeu: 4.641 ± 0.318
1.492GluMet: 1.492 ± 0.212
3.214GluAsn: 3.214 ± 0.216
1.919GluPro: 1.919 ± 0.213
2.263GluGln: 2.263 ± 0.19
2.46GluArg: 2.46 ± 0.236
2.919GluSer: 2.919 ± 0.226
4.018GluThr: 4.018 ± 0.37
5.461GluVal: 5.461 ± 0.321
1.099GluTrp: 1.099 ± 0.143
3.017GluTyr: 3.017 ± 0.234
0.0GluXaa: 0.0 ± 0.0
Phe
2.755PheAla: 2.755 ± 0.229
0.41PheCys: 0.41 ± 0.091
3.772PheAsp: 3.772 ± 0.256
2.935PheGlu: 2.935 ± 0.236
1.623PhePhe: 1.623 ± 0.186
2.919PheGly: 2.919 ± 0.252
0.672PheHis: 0.672 ± 0.124
2.329PheIle: 2.329 ± 0.218
2.411PheLys: 2.411 ± 0.188
3.017PheLeu: 3.017 ± 0.233
0.886PheMet: 0.886 ± 0.153
2.985PheAsn: 2.985 ± 0.274
1.886PhePro: 1.886 ± 0.171
1.558PheGln: 1.558 ± 0.209
1.902PheArg: 1.902 ± 0.172
3.05PheSer: 3.05 ± 0.23
3.477PheThr: 3.477 ± 0.302
3.116PheVal: 3.116 ± 0.308
0.459PheTrp: 0.459 ± 0.096
1.919PheTyr: 1.919 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
5.772GlyAla: 5.772 ± 0.481
0.574GlyCys: 0.574 ± 0.099
5.215GlyAsp: 5.215 ± 0.463
4.526GlyGlu: 4.526 ± 0.317
2.624GlyPhe: 2.624 ± 0.214
10.249GlyGly: 10.249 ± 1.814
0.918GlyHis: 0.918 ± 0.128
4.1GlyIle: 4.1 ± 0.329
3.526GlyLys: 3.526 ± 0.271
4.608GlyLeu: 4.608 ± 0.376
1.394GlyMet: 1.394 ± 0.171
5.198GlyAsn: 5.198 ± 0.46
1.935GlyPro: 1.935 ± 0.182
2.935GlyGln: 2.935 ± 0.207
2.968GlyArg: 2.968 ± 0.198
7.412GlySer: 7.412 ± 0.572
7.068GlyThr: 7.068 ± 0.613
5.707GlyVal: 5.707 ± 0.357
1.214GlyTrp: 1.214 ± 0.195
3.477GlyTyr: 3.477 ± 0.245
0.0GlyXaa: 0.0 ± 0.0
His
0.804HisAla: 0.804 ± 0.105
0.115HisCys: 0.115 ± 0.045
0.935HisAsp: 0.935 ± 0.131
0.886HisGlu: 0.886 ± 0.151
0.722HisPhe: 0.722 ± 0.105
1.017HisGly: 1.017 ± 0.15
0.426HisHis: 0.426 ± 0.098
0.902HisIle: 0.902 ± 0.136
0.902HisLys: 0.902 ± 0.151
1.214HisLeu: 1.214 ± 0.195
0.279HisMet: 0.279 ± 0.079
0.738HisAsn: 0.738 ± 0.105
0.886HisPro: 0.886 ± 0.119
0.525HisGln: 0.525 ± 0.11
0.64HisArg: 0.64 ± 0.113
0.968HisSer: 0.968 ± 0.109
0.82HisThr: 0.82 ± 0.113
0.754HisVal: 0.754 ± 0.129
0.23HisTrp: 0.23 ± 0.075
0.935HisTyr: 0.935 ± 0.146
0.0HisXaa: 0.0 ± 0.0
Ile
4.214IleAla: 4.214 ± 0.28
0.59IleCys: 0.59 ± 0.104
4.821IleAsp: 4.821 ± 0.269
3.821IleGlu: 3.821 ± 0.238
2.361IlePhe: 2.361 ± 0.207
4.247IleGly: 4.247 ± 0.277
0.886IleHis: 0.886 ± 0.144
3.919IleIle: 3.919 ± 0.294
3.296IleLys: 3.296 ± 0.329
4.362IleLeu: 4.362 ± 0.265
0.853IleMet: 0.853 ± 0.147
3.772IleAsn: 3.772 ± 0.292
2.624IlePro: 2.624 ± 0.206
2.853IleGln: 2.853 ± 0.215
2.673IleArg: 2.673 ± 0.179
4.739IleSer: 4.739 ± 0.3
5.789IleThr: 5.789 ± 0.511
3.706IleVal: 3.706 ± 0.27
0.607IleTrp: 0.607 ± 0.106
2.214IleTyr: 2.214 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
3.198LysAla: 3.198 ± 0.276
0.672LysCys: 0.672 ± 0.126
3.28LysAsp: 3.28 ± 0.327
4.214LysGlu: 4.214 ± 0.368
2.853LysPhe: 2.853 ± 0.286
3.099LysGly: 3.099 ± 0.264
0.754LysHis: 0.754 ± 0.132
3.936LysIle: 3.936 ± 0.315
3.887LysLys: 3.887 ± 0.452
4.116LysLeu: 4.116 ± 0.276
1.443LysMet: 1.443 ± 0.222
2.935LysAsn: 2.935 ± 0.211
1.541LysPro: 1.541 ± 0.2
2.132LysGln: 2.132 ± 0.212
2.558LysArg: 2.558 ± 0.208
3.001LysSer: 3.001 ± 0.277
3.28LysThr: 3.28 ± 0.303
3.624LysVal: 3.624 ± 0.248
0.574LysTrp: 0.574 ± 0.103
3.149LysTyr: 3.149 ± 0.268
0.0LysXaa: 0.0 ± 0.0
Leu
4.723LeuAla: 4.723 ± 0.289
0.59LeuCys: 0.59 ± 0.108
5.28LeuAsp: 5.28 ± 0.316
5.149LeuGlu: 5.149 ± 0.332
2.788LeuPhe: 2.788 ± 0.262
4.46LeuGly: 4.46 ± 0.323
1.05LeuHis: 1.05 ± 0.137
4.034LeuIle: 4.034 ± 0.249
3.985LeuLys: 3.985 ± 0.355
5.018LeuLeu: 5.018 ± 0.401
1.476LeuMet: 1.476 ± 0.195
5.067LeuAsn: 5.067 ± 0.299
3.132LeuPro: 3.132 ± 0.211
2.87LeuGln: 2.87 ± 0.248
3.132LeuArg: 3.132 ± 0.197
4.821LeuSer: 4.821 ± 0.385
5.297LeuThr: 5.297 ± 0.376
4.247LeuVal: 4.247 ± 0.267
0.59LeuTrp: 0.59 ± 0.089
3.001LeuTyr: 3.001 ± 0.221
0.0LeuXaa: 0.0 ± 0.0
Met
1.492MetAla: 1.492 ± 0.203
0.164MetCys: 0.164 ± 0.063
1.099MetAsp: 1.099 ± 0.171
1.115MetGlu: 1.115 ± 0.164
0.754MetPhe: 0.754 ± 0.123
1.05MetGly: 1.05 ± 0.152
0.246MetHis: 0.246 ± 0.068
1.066MetIle: 1.066 ± 0.145
1.64MetLys: 1.64 ± 0.256
1.591MetLeu: 1.591 ± 0.215
0.64MetMet: 0.64 ± 0.133
1.066MetAsn: 1.066 ± 0.158
1.0MetPro: 1.0 ± 0.136
0.64MetGln: 0.64 ± 0.125
0.656MetArg: 0.656 ± 0.109
1.214MetSer: 1.214 ± 0.192
1.279MetThr: 1.279 ± 0.179
1.148MetVal: 1.148 ± 0.139
0.262MetTrp: 0.262 ± 0.061
0.59MetTyr: 0.59 ± 0.103
0.0MetXaa: 0.0 ± 0.0
Asn
3.411AsnAla: 3.411 ± 0.297
0.492AsnCys: 0.492 ± 0.101
3.477AsnAsp: 3.477 ± 0.275
3.378AsnGlu: 3.378 ± 0.25
3.017AsnPhe: 3.017 ± 0.195
4.87AsnGly: 4.87 ± 0.371
1.082AsnHis: 1.082 ± 0.127
4.198AsnIle: 4.198 ± 0.292
2.706AsnLys: 2.706 ± 0.207
4.051AsnLeu: 4.051 ± 0.299
1.033AsnMet: 1.033 ± 0.172
3.427AsnAsn: 3.427 ± 0.287
3.05AsnPro: 3.05 ± 0.208
2.033AsnGln: 2.033 ± 0.211
2.837AsnArg: 2.837 ± 0.269
3.985AsnSer: 3.985 ± 0.328
4.378AsnThr: 4.378 ± 0.402
4.149AsnVal: 4.149 ± 0.258
0.804AsnTrp: 0.804 ± 0.099
2.739AsnTyr: 2.739 ± 0.188
0.0AsnXaa: 0.0 ± 0.0
Pro
3.083ProAla: 3.083 ± 0.35
0.18ProCys: 0.18 ± 0.059
3.017ProAsp: 3.017 ± 0.194
2.788ProGlu: 2.788 ± 0.236
1.689ProPhe: 1.689 ± 0.155
3.329ProGly: 3.329 ± 0.284
0.607ProHis: 0.607 ± 0.112
2.148ProIle: 2.148 ± 0.173
1.787ProLys: 1.787 ± 0.263
2.64ProLeu: 2.64 ± 0.186
0.574ProMet: 0.574 ± 0.111
2.23ProAsn: 2.23 ± 0.193
1.755ProPro: 1.755 ± 0.221
1.656ProGln: 1.656 ± 0.18
1.296ProArg: 1.296 ± 0.165
3.132ProSer: 3.132 ± 0.222
3.591ProThr: 3.591 ± 0.234
2.673ProVal: 2.673 ± 0.229
0.426ProTrp: 0.426 ± 0.091
1.623ProTyr: 1.623 ± 0.193
0.0ProXaa: 0.0 ± 0.0
Gln
1.902GlnAla: 1.902 ± 0.221
0.295GlnCys: 0.295 ± 0.076
2.083GlnAsp: 2.083 ± 0.191
2.312GlnGlu: 2.312 ± 0.208
2.05GlnPhe: 2.05 ± 0.218
2.378GlnGly: 2.378 ± 0.215
0.672GlnHis: 0.672 ± 0.122
2.345GlnIle: 2.345 ± 0.23
2.296GlnLys: 2.296 ± 0.241
2.968GlnLeu: 2.968 ± 0.211
0.886GlnMet: 0.886 ± 0.139
2.05GlnAsn: 2.05 ± 0.177
1.378GlnPro: 1.378 ± 0.143
1.378GlnGln: 1.378 ± 0.169
1.837GlnArg: 1.837 ± 0.154
2.755GlnSer: 2.755 ± 0.224
2.296GlnThr: 2.296 ± 0.18
2.443GlnVal: 2.443 ± 0.204
0.623GlnTrp: 0.623 ± 0.11
1.853GlnTyr: 1.853 ± 0.171
0.0GlnXaa: 0.0 ± 0.0
Arg
3.132ArgAla: 3.132 ± 0.255
0.312ArgCys: 0.312 ± 0.078
2.624ArgAsp: 2.624 ± 0.237
2.411ArgGlu: 2.411 ± 0.254
2.214ArgPhe: 2.214 ± 0.214
2.968ArgGly: 2.968 ± 0.249
0.558ArgHis: 0.558 ± 0.126
2.64ArgIle: 2.64 ± 0.205
2.575ArgLys: 2.575 ± 0.268
3.198ArgLeu: 3.198 ± 0.248
0.935ArgMet: 0.935 ± 0.148
2.083ArgAsn: 2.083 ± 0.178
1.459ArgPro: 1.459 ± 0.163
1.623ArgGln: 1.623 ± 0.172
2.181ArgArg: 2.181 ± 0.189
2.033ArgSer: 2.033 ± 0.213
2.378ArgThr: 2.378 ± 0.17
2.804ArgVal: 2.804 ± 0.19
0.607ArgTrp: 0.607 ± 0.102
2.279ArgTyr: 2.279 ± 0.226
0.0ArgXaa: 0.0 ± 0.0
Ser
5.034SerAla: 5.034 ± 0.354
0.459SerCys: 0.459 ± 0.097
4.329SerAsp: 4.329 ± 0.232
3.427SerGlu: 3.427 ± 0.272
3.017SerPhe: 3.017 ± 0.207
8.527SerGly: 8.527 ± 0.748
0.804SerHis: 0.804 ± 0.1
4.739SerIle: 4.739 ± 0.354
3.46SerLys: 3.46 ± 0.309
4.411SerLeu: 4.411 ± 0.3
1.05SerMet: 1.05 ± 0.164
4.362SerAsn: 4.362 ± 0.346
2.804SerPro: 2.804 ± 0.194
2.165SerGln: 2.165 ± 0.149
2.378SerArg: 2.378 ± 0.21
5.379SerSer: 5.379 ± 0.567
4.887SerThr: 4.887 ± 0.466
4.674SerVal: 4.674 ± 0.467
0.59SerTrp: 0.59 ± 0.102
3.067SerTyr: 3.067 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
5.395ThrAla: 5.395 ± 0.447
0.476ThrCys: 0.476 ± 0.09
4.739ThrAsp: 4.739 ± 0.338
3.985ThrGlu: 3.985 ± 0.26
3.591ThrPhe: 3.591 ± 0.307
6.838ThrGly: 6.838 ± 0.634
1.0ThrHis: 1.0 ± 0.108
5.1ThrIle: 5.1 ± 0.476
3.181ThrLys: 3.181 ± 0.234
5.559ThrLeu: 5.559 ± 0.317
0.787ThrMet: 0.787 ± 0.119
4.001ThrAsn: 4.001 ± 0.293
3.575ThrPro: 3.575 ± 0.232
2.673ThrGln: 2.673 ± 0.198
2.46ThrArg: 2.46 ± 0.185
5.494ThrSer: 5.494 ± 0.422
6.166ThrThr: 6.166 ± 0.509
5.051ThrVal: 5.051 ± 0.442
0.804ThrTrp: 0.804 ± 0.126
3.345ThrTyr: 3.345 ± 0.226
0.0ThrXaa: 0.0 ± 0.0
Val
4.772ValAla: 4.772 ± 0.313
0.607ValCys: 0.607 ± 0.128
5.756ValAsp: 5.756 ± 0.376
4.149ValGlu: 4.149 ± 0.314
2.903ValPhe: 2.903 ± 0.219
4.87ValGly: 4.87 ± 0.421
0.787ValHis: 0.787 ± 0.135
4.149ValIle: 4.149 ± 0.343
3.854ValLys: 3.854 ± 0.28
4.608ValLeu: 4.608 ± 0.267
1.164ValMet: 1.164 ± 0.178
3.837ValAsn: 3.837 ± 0.271
2.607ValPro: 2.607 ± 0.193
2.312ValGln: 2.312 ± 0.187
2.886ValArg: 2.886 ± 0.217
5.592ValSer: 5.592 ± 0.418
4.624ValThr: 4.624 ± 0.371
5.1ValVal: 5.1 ± 0.434
0.558ValTrp: 0.558 ± 0.094
2.394ValTyr: 2.394 ± 0.214
0.0ValXaa: 0.0 ± 0.0
Trp
0.722TrpAla: 0.722 ± 0.113
0.148TrpCys: 0.148 ± 0.054
1.033TrpAsp: 1.033 ± 0.146
0.738TrpGlu: 0.738 ± 0.148
0.558TrpPhe: 0.558 ± 0.096
0.771TrpGly: 0.771 ± 0.114
0.246TrpHis: 0.246 ± 0.064
0.623TrpIle: 0.623 ± 0.103
0.771TrpLys: 0.771 ± 0.113
0.804TrpLeu: 0.804 ± 0.137
0.246TrpMet: 0.246 ± 0.061
0.918TrpAsn: 0.918 ± 0.103
0.262TrpPro: 0.262 ± 0.071
0.459TrpGln: 0.459 ± 0.105
0.607TrpArg: 0.607 ± 0.1
0.607TrpSer: 0.607 ± 0.113
0.951TrpThr: 0.951 ± 0.118
0.771TrpVal: 0.771 ± 0.128
0.131TrpTrp: 0.131 ± 0.041
0.574TrpTyr: 0.574 ± 0.091
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.558TyrAla: 2.558 ± 0.192
0.508TyrCys: 0.508 ± 0.095
3.345TyrAsp: 3.345 ± 0.221
2.64TyrGlu: 2.64 ± 0.261
2.181TyrPhe: 2.181 ± 0.248
2.624TyrGly: 2.624 ± 0.208
0.738TyrHis: 0.738 ± 0.11
2.886TyrIle: 2.886 ± 0.237
2.427TyrLys: 2.427 ± 0.223
3.542TyrLeu: 3.542 ± 0.24
0.82TyrMet: 0.82 ± 0.136
2.919TyrAsn: 2.919 ± 0.249
1.951TyrPro: 1.951 ± 0.193
1.869TyrGln: 1.869 ± 0.16
2.017TyrArg: 2.017 ± 0.221
2.722TyrSer: 2.722 ± 0.22
3.46TyrThr: 3.46 ± 0.245
3.099TyrVal: 3.099 ± 0.249
0.459TyrTrp: 0.459 ± 0.093
2.099TyrTyr: 2.099 ± 0.223
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 237 proteins (60981 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski