Amycolatopsis xylanica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8491 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3EAC7|A0A1H3EAC7_9PSEU dTMP kinase OS=Amycolatopsis xylanica OX=589385 GN=SAMN05421504_103697 PE=4 SV=1
MM1 pKa = 7.6AVTLDD6 pKa = 3.61AQLLEE11 pKa = 4.38ILACPSPDD19 pKa = 3.53HH20 pKa = 6.74APLRR24 pKa = 11.84AGSPGDD30 pKa = 3.87PEE32 pKa = 6.21ADD34 pKa = 2.81ALTCTEE40 pKa = 4.44CGRR43 pKa = 11.84VYY45 pKa = 10.34PVRR48 pKa = 11.84DD49 pKa = 4.78GIPVLLLDD57 pKa = 4.71EE58 pKa = 4.8ATTPDD63 pKa = 3.31ASEE66 pKa = 4.0TSSADD71 pKa = 3.26GAA73 pKa = 4.09

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2UUD4|A0A1H2UUD4_9PSEU Polysaccharide lyase OS=Amycolatopsis xylanica OX=589385 GN=SAMN05421504_1011002 PE=4 SV=1
MM1 pKa = 6.16VTRR4 pKa = 11.84VLVTVLVLVFAVLGVMVTPGGALTGWVMAGLAAGVLVWQAPARR47 pKa = 11.84VQRR50 pKa = 11.84MIKK53 pKa = 9.76VRR55 pKa = 11.84APVVGAAVFVGGLVIMGMAAVLGAAVTVWLLVLAALAVAVSFRR98 pKa = 11.84LRR100 pKa = 11.84GRR102 pKa = 11.84IHH104 pKa = 6.68

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8491

0

8491

2874613

29

6600

338.5

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.997 ± 0.043

0.778 ± 0.008

5.938 ± 0.021

5.67 ± 0.032

3.082 ± 0.014

9.124 ± 0.025

2.143 ± 0.012

3.767 ± 0.015

2.716 ± 0.022

10.548 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.716 ± 0.009

2.124 ± 0.019

5.715 ± 0.023

2.837 ± 0.015

7.234 ± 0.031

5.358 ± 0.02

6.071 ± 0.024

8.542 ± 0.023

1.542 ± 0.01

2.098 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski