Hippeastrum latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4F975|Q4F975_9VIRU 7 kDa protein OS=Hippeastrum latent virus OX=335963 GN=TGB3 PE=3 SV=1
MM1 pKa = 6.62ATKK4 pKa = 10.56VADD7 pKa = 3.8NPSDD11 pKa = 3.99PNVGEE16 pKa = 4.27PKK18 pKa = 9.33ATKK21 pKa = 10.15PGDD24 pKa = 3.89NVDD27 pKa = 3.68PAAAADD33 pKa = 4.13LFDD36 pKa = 5.18DD37 pKa = 4.15ARR39 pKa = 11.84LMEE42 pKa = 4.53DD43 pKa = 3.27QIEE46 pKa = 4.05EE47 pKa = 3.78RR48 pKa = 11.84MARR51 pKa = 11.84LRR53 pKa = 11.84EE54 pKa = 3.98FLMKK58 pKa = 9.54QQRR61 pKa = 11.84AVQVTNPSFEE71 pKa = 4.33LGRR74 pKa = 11.84PKK76 pKa = 10.81LKK78 pKa = 10.2MLDD81 pKa = 3.74SVRR84 pKa = 11.84SDD86 pKa = 3.1PTNLYY91 pKa = 10.11NKK93 pKa = 8.23PTIDD97 pKa = 3.28QLCRR101 pKa = 11.84IRR103 pKa = 11.84PKK105 pKa = 10.68SISNNMATSQDD116 pKa = 3.27MAAITVAIEE125 pKa = 4.06SLGVPSEE132 pKa = 4.15KK133 pKa = 10.48VQTVIIQAVAYY144 pKa = 9.64CKK146 pKa = 10.29DD147 pKa = 3.63ASSSAYY153 pKa = 10.3LDD155 pKa = 3.56PQGTFEE161 pKa = 4.35WEE163 pKa = 4.13GGAIMADD170 pKa = 3.03AVLAILKK177 pKa = 9.88RR178 pKa = 11.84DD179 pKa = 3.26AGTLRR184 pKa = 11.84RR185 pKa = 11.84VCRR188 pKa = 11.84LYY190 pKa = 11.2APVTWNHH197 pKa = 5.31MLAHH201 pKa = 6.71NAPPSDD207 pKa = 3.31WAAMGFQYY215 pKa = 9.65TEE217 pKa = 3.6RR218 pKa = 11.84FAAFDD223 pKa = 3.52CFDD226 pKa = 3.69YY227 pKa = 11.51VEE229 pKa = 4.45NQAAVQPFEE238 pKa = 3.92GLIRR242 pKa = 11.84RR243 pKa = 11.84PTPAEE248 pKa = 4.15KK249 pKa = 9.49IAHH252 pKa = 5.2NTHH255 pKa = 5.92KK256 pKa = 10.71RR257 pKa = 11.84IALDD261 pKa = 3.21RR262 pKa = 11.84ANRR265 pKa = 11.84NEE267 pKa = 4.0RR268 pKa = 11.84FSSLEE273 pKa = 3.68AEE275 pKa = 4.22ITGGRR280 pKa = 11.84LGPEE284 pKa = 3.92IEE286 pKa = 3.77RR287 pKa = 11.84SYY289 pKa = 11.07WKK291 pKa = 9.11TT292 pKa = 3.12

Molecular weight: 32.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4F974|Q4F974_9VIRU Capsid protein OS=Hippeastrum latent virus OX=335963 GN=CP PE=3 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84VDD4 pKa = 4.37ILIALCVNRR13 pKa = 11.84EE14 pKa = 3.74FNKK17 pKa = 10.36RR18 pKa = 11.84GEE20 pKa = 4.09HH21 pKa = 6.45HH22 pKa = 7.18IPLAIYY28 pKa = 8.66IAKK31 pKa = 10.06RR32 pKa = 11.84VGPPLVSTGTSTYY45 pKa = 10.35ARR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84AASIGRR56 pKa = 11.84CHH58 pKa = 6.37RR59 pKa = 11.84CYY61 pKa = 10.06RR62 pKa = 11.84VYY64 pKa = 10.7PPFWWTTRR72 pKa = 11.84CDD74 pKa = 3.51NKK76 pKa = 10.26TCVPGISYY84 pKa = 10.01KK85 pKa = 10.89AEE87 pKa = 3.68VEE89 pKa = 4.85SYY91 pKa = 10.36VKK93 pKa = 10.17WGVAEE98 pKa = 5.45AIPHH102 pKa = 5.98FKK104 pKa = 10.68LL105 pKa = 3.96

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2765

59

1971

460.8

51.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.21 ± 1.033

3.436 ± 0.553

4.774 ± 0.632

7.052 ± 0.914

5.244 ± 0.519

6.076 ± 0.423

2.351 ± 0.384

5.353 ± 0.748

5.859 ± 0.762

8.969 ± 0.671

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.493

3.508 ± 0.509

4.159 ± 1.065

3.183 ± 0.377

6.799 ± 0.514

6.438 ± 0.342

4.774 ± 0.621

7.052 ± 0.561

1.121 ± 0.212

3.183 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski