Nostoc linckia NIES-25

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; Nostoc linckia

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z4L9R1|A0A1Z4L9R1_NOSLI Uncharacterized protein OS=Nostoc linckia NIES-25 OX=1091006 GN=NIES25_44150 PE=4 SV=1
MM1 pKa = 7.57ANINGTKK8 pKa = 10.23NDD10 pKa = 3.64DD11 pKa = 3.88TLNGSNLPDD20 pKa = 4.43TINGLLGNDD29 pKa = 3.98TITAKK34 pKa = 10.49EE35 pKa = 4.22GNDD38 pKa = 3.64TLSGGSGKK46 pKa = 8.81DD47 pKa = 2.98TFIYY51 pKa = 10.62KK52 pKa = 10.56LGDD55 pKa = 3.41GTDD58 pKa = 4.71FISDD62 pKa = 3.84FGGIGKK68 pKa = 7.92GTNPTAAVIAEE79 pKa = 4.11VDD81 pKa = 3.47TLIFQSYY88 pKa = 9.97GLTARR93 pKa = 11.84NLLLTQNGTNLEE105 pKa = 4.17ITFEE109 pKa = 4.15RR110 pKa = 11.84VDD112 pKa = 3.57DD113 pKa = 4.64AKK115 pKa = 11.4VILQNFALEE124 pKa = 4.15NLDD127 pKa = 3.95NLSKK131 pKa = 10.27STTATVDD138 pKa = 2.93LGNIVFYY145 pKa = 10.27EE146 pKa = 4.21QTSTTDD152 pKa = 2.95SFDD155 pKa = 3.78VFDD158 pKa = 5.19ANSNQSTIFNKK169 pKa = 8.77NTVTFFNDD177 pKa = 2.75LNNNVKK183 pKa = 10.57GFDD186 pKa = 3.67NSDD189 pKa = 3.5DD190 pKa = 4.09VINGQGGNDD199 pKa = 4.06KK200 pKa = 10.69IRR202 pKa = 11.84GLSGNDD208 pKa = 3.55LLRR211 pKa = 11.84GGTGNDD217 pKa = 3.47TLIGGAGNDD226 pKa = 3.92TLVGGAGNNLLNGDD240 pKa = 4.19IDD242 pKa = 5.59DD243 pKa = 5.35DD244 pKa = 4.2LLSIYY249 pKa = 10.16SYY251 pKa = 11.6YY252 pKa = 10.63DD253 pKa = 2.8IYY255 pKa = 11.21DD256 pKa = 3.58YY257 pKa = 10.99PPYY260 pKa = 10.54KK261 pKa = 10.59NNPRR265 pKa = 11.84SDD267 pKa = 3.65GNNTLNGGAGNDD279 pKa = 3.5TLIASRR285 pKa = 11.84STGNNLLDD293 pKa = 4.53GGDD296 pKa = 4.29GNDD299 pKa = 3.58SLDD302 pKa = 3.1ISGYY306 pKa = 10.29DD307 pKa = 3.39EE308 pKa = 4.65YY309 pKa = 11.64VLHH312 pKa = 7.04SDD314 pKa = 4.56YY315 pKa = 11.47SALGNNTLKK324 pKa = 11.02GGAGFDD330 pKa = 3.67NLNANYY336 pKa = 10.57SSGDD340 pKa = 3.45NLLSGGADD348 pKa = 3.42NDD350 pKa = 3.69NLSASGSKK358 pKa = 10.5GNNLLSGGDD367 pKa = 3.52GNDD370 pKa = 3.47YY371 pKa = 11.08VSVSGQYY378 pKa = 9.73QYY380 pKa = 11.75EE381 pKa = 3.89FDD383 pKa = 3.59SYY385 pKa = 11.97GRR387 pKa = 11.84FPTFNFDD394 pKa = 4.58SRR396 pKa = 11.84SLGNNTLNGGNGNDD410 pKa = 3.62TLSASGSKK418 pKa = 10.48GNNLLSGGNGNDD430 pKa = 3.48YY431 pKa = 11.44LNISGSYY438 pKa = 9.51SYY440 pKa = 11.35YY441 pKa = 9.82SNEE444 pKa = 3.99YY445 pKa = 9.19YY446 pKa = 10.51DD447 pKa = 5.65EE448 pKa = 4.53YY449 pKa = 11.3DD450 pKa = 2.91QTYY453 pKa = 9.96YY454 pKa = 11.32YY455 pKa = 10.31SASEE459 pKa = 3.87NDD461 pKa = 3.54SRR463 pKa = 11.84SLGNNTLKK471 pKa = 10.96GGAGDD476 pKa = 3.82DD477 pKa = 3.74TLNAGGSTGNNLLSGGDD494 pKa = 3.58GNDD497 pKa = 3.55YY498 pKa = 10.81ISALNASGNNIIKK511 pKa = 10.36GDD513 pKa = 3.94YY514 pKa = 11.34GNDD517 pKa = 3.32TLNGGFGNDD526 pKa = 3.23TLYY529 pKa = 11.09GGSGIDD535 pKa = 3.17TFGFKK540 pKa = 10.44SFNEE544 pKa = 4.27GLDD547 pKa = 3.41TIYY550 pKa = 11.13DD551 pKa = 3.95FNATNEE557 pKa = 4.25VISIAFGYY565 pKa = 10.73DD566 pKa = 3.52FGAKK570 pKa = 9.44GVLSSTQFTIGTSATTNAQRR590 pKa = 11.84LIYY593 pKa = 10.59NSTTGALFFDD603 pKa = 4.56EE604 pKa = 5.68DD605 pKa = 4.38GSGSTFSQLQFAQLSAGLLLTNNNFVIII633 pKa = 4.2

Molecular weight: 66.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z4LF21|A0A1Z4LF21_NOSLI 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein OS=Nostoc linckia NIES-25 OX=1091006 GN=NIES25_63120 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.03PNYY5 pKa = 9.07RR6 pKa = 11.84RR7 pKa = 11.84CISCRR12 pKa = 11.84KK13 pKa = 9.67LGLKK17 pKa = 10.18EE18 pKa = 3.92EE19 pKa = 4.62FWRR22 pKa = 11.84IVRR25 pKa = 11.84VFPSGKK31 pKa = 8.66VQLDD35 pKa = 3.07RR36 pKa = 11.84GMGRR40 pKa = 11.84SAYY43 pKa = 9.3ICPQVSCLQAAQKK56 pKa = 10.5KK57 pKa = 8.83NRR59 pKa = 11.84LGRR62 pKa = 11.84SLHH65 pKa = 6.06ASVPEE70 pKa = 3.79TLYY73 pKa = 10.86QSLSQRR79 pKa = 11.84LASNNTQNQII89 pKa = 3.34

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6881

0

6881

2248921

29

7234

326.8

36.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.05 ± 0.03

0.942 ± 0.011

4.88 ± 0.024

6.299 ± 0.034

4.032 ± 0.019

6.685 ± 0.051

1.73 ± 0.014

6.957 ± 0.025

4.924 ± 0.025

10.958 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.694 ± 0.013

4.728 ± 0.036

4.555 ± 0.025

5.379 ± 0.032

4.982 ± 0.026

6.5 ± 0.026

5.726 ± 0.033

6.529 ± 0.023

1.375 ± 0.014

3.077 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski