Amino acid dipepetide frequency for Nostoc linckia NIES-25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.368AlaAla: 7.368 ± 0.083
0.783AlaCys: 0.783 ± 0.017
3.915AlaAsp: 3.915 ± 0.048
5.074AlaGlu: 5.074 ± 0.062
2.987AlaPhe: 2.987 ± 0.04
5.449AlaGly: 5.449 ± 0.072
1.234AlaHis: 1.234 ± 0.023
7.058AlaIle: 7.058 ± 0.073
4.2AlaLys: 4.2 ± 0.047
8.414AlaLeu: 8.414 ± 0.066
1.563AlaMet: 1.563 ± 0.031
3.736AlaAsn: 3.736 ± 0.058
2.712AlaPro: 2.712 ± 0.042
4.158AlaGln: 4.158 ± 0.05
3.466AlaArg: 3.466 ± 0.046
4.982AlaSer: 4.982 ± 0.062
4.712AlaThr: 4.712 ± 0.067
5.391AlaVal: 5.391 ± 0.057
0.967AlaTrp: 0.967 ± 0.021
2.336AlaTyr: 2.336 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
0.591CysAla: 0.591 ± 0.017
0.156CysCys: 0.156 ± 0.007
0.531CysAsp: 0.531 ± 0.018
0.515CysGlu: 0.515 ± 0.018
0.393CysPhe: 0.393 ± 0.013
0.734CysGly: 0.734 ± 0.019
0.256CysHis: 0.256 ± 0.011
0.606CysIle: 0.606 ± 0.017
0.358CysLys: 0.358 ± 0.013
1.07CysLeu: 1.07 ± 0.026
0.147CysMet: 0.147 ± 0.008
0.4CysAsn: 0.4 ± 0.013
0.509CysPro: 0.509 ± 0.016
0.565CysGln: 0.565 ± 0.018
0.474CysArg: 0.474 ± 0.015
0.595CysSer: 0.595 ± 0.017
0.451CysThr: 0.451 ± 0.016
0.558CysVal: 0.558 ± 0.017
0.144CysTrp: 0.144 ± 0.008
0.365CysTyr: 0.365 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.685AspAla: 3.685 ± 0.044
0.494AspCys: 0.494 ± 0.014
2.315AspAsp: 2.315 ± 0.045
3.121AspGlu: 3.121 ± 0.046
2.406AspPhe: 2.406 ± 0.035
3.533AspGly: 3.533 ± 0.064
0.502AspHis: 0.502 ± 0.017
3.603AspIle: 3.603 ± 0.047
2.329AspLys: 2.329 ± 0.034
5.564AspLeu: 5.564 ± 0.054
0.687AspMet: 0.687 ± 0.018
2.193AspAsn: 2.193 ± 0.038
2.138AspPro: 2.138 ± 0.034
1.248AspGln: 1.248 ± 0.025
3.503AspArg: 3.503 ± 0.045
3.051AspSer: 3.051 ± 0.039
2.666AspThr: 2.666 ± 0.05
3.008AspVal: 3.008 ± 0.035
0.873AspTrp: 0.873 ± 0.02
1.88AspTyr: 1.88 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
5.355GluAla: 5.355 ± 0.059
0.481GluCys: 0.481 ± 0.017
2.79GluAsp: 2.79 ± 0.042
4.163GluGlu: 4.163 ± 0.06
2.519GluPhe: 2.519 ± 0.037
3.066GluGly: 3.066 ± 0.04
1.006GluHis: 1.006 ± 0.024
5.199GluIle: 5.199 ± 0.057
3.623GluLys: 3.623 ± 0.049
7.111GluLeu: 7.111 ± 0.079
1.225GluMet: 1.225 ± 0.026
3.052GluAsn: 3.052 ± 0.039
2.329GluPro: 2.329 ± 0.038
3.734GluGln: 3.734 ± 0.045
3.364GluArg: 3.364 ± 0.05
3.485GluSer: 3.485 ± 0.045
3.44GluThr: 3.44 ± 0.042
4.324GluVal: 4.324 ± 0.051
0.771GluTrp: 0.771 ± 0.02
1.949GluTyr: 1.949 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.161PheAla: 3.161 ± 0.046
0.528PheCys: 0.528 ± 0.016
2.289PheAsp: 2.289 ± 0.035
2.295PheGlu: 2.295 ± 0.035
1.737PhePhe: 1.737 ± 0.033
2.999PheGly: 2.999 ± 0.041
0.727PheHis: 0.727 ± 0.019
2.592PheIle: 2.592 ± 0.038
1.696PheLys: 1.696 ± 0.03
4.205PheLeu: 4.205 ± 0.047
0.688PheMet: 0.688 ± 0.018
1.942PheAsn: 1.942 ± 0.046
1.781PhePro: 1.781 ± 0.028
1.873PheGln: 1.873 ± 0.028
1.745PheArg: 1.745 ± 0.031
3.055PheSer: 3.055 ± 0.046
2.356PheThr: 2.356 ± 0.038
2.553PheVal: 2.553 ± 0.037
0.691PheTrp: 0.691 ± 0.02
1.405PheTyr: 1.405 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
4.757GlyAla: 4.757 ± 0.064
0.702GlyCys: 0.702 ± 0.019
3.437GlyAsp: 3.437 ± 0.055
4.038GlyGlu: 4.038 ± 0.047
2.965GlyPhe: 2.965 ± 0.047
5.101GlyGly: 5.101 ± 0.114
1.171GlyHis: 1.171 ± 0.027
5.203GlyIle: 5.203 ± 0.058
4.047GlyLys: 4.047 ± 0.06
6.766GlyLeu: 6.766 ± 0.067
1.332GlyMet: 1.332 ± 0.024
3.523GlyAsn: 3.523 ± 0.103
1.22GlyPro: 1.22 ± 0.028
2.944GlyGln: 2.944 ± 0.045
3.266GlyArg: 3.266 ± 0.045
4.215GlySer: 4.215 ± 0.075
4.09GlyThr: 4.09 ± 0.081
4.683GlyVal: 4.683 ± 0.055
1.043GlyTrp: 1.043 ± 0.024
2.345GlyTyr: 2.345 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
1.016HisAla: 1.016 ± 0.023
0.217HisCys: 0.217 ± 0.01
0.681HisAsp: 0.681 ± 0.02
0.944HisGlu: 0.944 ± 0.022
0.74HisPhe: 0.74 ± 0.022
1.008HisGly: 1.008 ± 0.023
0.538HisHis: 0.538 ± 0.018
1.089HisIle: 1.089 ± 0.025
0.771HisLys: 0.771 ± 0.021
2.137HisLeu: 2.137 ± 0.036
0.229HisMet: 0.229 ± 0.011
0.76HisAsn: 0.76 ± 0.021
1.231HisPro: 1.231 ± 0.025
1.146HisGln: 1.146 ± 0.024
1.072HisArg: 1.072 ± 0.022
1.103HisSer: 1.103 ± 0.026
0.912HisThr: 0.912 ± 0.023
0.767HisVal: 0.767 ± 0.023
0.319HisTrp: 0.319 ± 0.011
0.623HisTyr: 0.623 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.389IleAla: 7.389 ± 0.075
0.755IleCys: 0.755 ± 0.022
3.816IleAsp: 3.816 ± 0.046
4.475IleGlu: 4.475 ± 0.056
2.749IlePhe: 2.749 ± 0.042
4.652IleGly: 4.652 ± 0.058
1.237IleHis: 1.237 ± 0.025
4.029IleIle: 4.029 ± 0.056
3.286IleLys: 3.286 ± 0.046
7.025IleLeu: 7.025 ± 0.066
0.818IleMet: 0.818 ± 0.021
3.374IleAsn: 3.374 ± 0.047
3.579IlePro: 3.579 ± 0.041
3.192IleGln: 3.192 ± 0.041
3.087IleArg: 3.087 ± 0.044
4.763IleSer: 4.763 ± 0.059
3.861IleThr: 3.861 ± 0.052
4.462IleVal: 4.462 ± 0.051
0.902IleTrp: 0.902 ± 0.023
2.12IleTyr: 2.12 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
3.977LysAla: 3.977 ± 0.055
0.337LysCys: 0.337 ± 0.013
2.252LysAsp: 2.252 ± 0.042
2.907LysGlu: 2.907 ± 0.05
1.976LysPhe: 1.976 ± 0.031
2.773LysGly: 2.773 ± 0.038
0.803LysHis: 0.803 ± 0.02
3.792LysIle: 3.792 ± 0.049
2.417LysLys: 2.417 ± 0.043
5.689LysLeu: 5.689 ± 0.059
0.939LysMet: 0.939 ± 0.021
2.389LysAsn: 2.389 ± 0.041
2.431LysPro: 2.431 ± 0.036
2.767LysGln: 2.767 ± 0.034
2.299LysArg: 2.299 ± 0.038
3.161LysSer: 3.161 ± 0.043
2.977LysThr: 2.977 ± 0.041
3.271LysVal: 3.271 ± 0.045
0.511LysTrp: 0.511 ± 0.017
1.571LysTyr: 1.571 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
9.473LeuAla: 9.473 ± 0.085
0.994LeuCys: 0.994 ± 0.022
5.163LeuAsp: 5.163 ± 0.048
7.436LeuGlu: 7.436 ± 0.073
3.878LeuPhe: 3.878 ± 0.05
7.53LeuGly: 7.53 ± 0.072
1.884LeuHis: 1.884 ± 0.03
6.939LeuIle: 6.939 ± 0.075
5.679LeuLys: 5.679 ± 0.058
11.417LeuLeu: 11.417 ± 0.107
1.96LeuMet: 1.96 ± 0.035
4.963LeuAsn: 4.963 ± 0.053
5.643LeuPro: 5.643 ± 0.066
5.796LeuGln: 5.796 ± 0.068
5.454LeuArg: 5.454 ± 0.058
7.547LeuSer: 7.547 ± 0.069
6.428LeuThr: 6.428 ± 0.06
7.163LeuVal: 7.163 ± 0.066
1.452LeuTrp: 1.452 ± 0.035
2.778LeuTyr: 2.778 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
1.514MetAla: 1.514 ± 0.028
0.121MetCys: 0.121 ± 0.008
0.653MetAsp: 0.653 ± 0.018
0.939MetGlu: 0.939 ± 0.021
0.53MetPhe: 0.53 ± 0.014
1.186MetGly: 1.186 ± 0.02
0.323MetHis: 0.323 ± 0.014
0.96MetIle: 0.96 ± 0.021
0.9MetLys: 0.9 ± 0.018
1.832MetLeu: 1.832 ± 0.032
0.396MetMet: 0.396 ± 0.014
0.829MetAsn: 0.829 ± 0.021
0.921MetPro: 0.921 ± 0.022
0.878MetGln: 0.878 ± 0.019
0.857MetArg: 0.857 ± 0.019
1.258MetSer: 1.258 ± 0.024
1.228MetThr: 1.228 ± 0.025
1.16MetVal: 1.16 ± 0.023
0.141MetTrp: 0.141 ± 0.008
0.321MetTyr: 0.321 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.202AsnAla: 3.202 ± 0.053
0.452AsnCys: 0.452 ± 0.018
2.13AsnAsp: 2.13 ± 0.072
2.09AsnGlu: 2.09 ± 0.035
2.225AsnPhe: 2.225 ± 0.036
3.085AsnGly: 3.085 ± 0.084
0.87AsnHis: 0.87 ± 0.023
3.201AsnIle: 3.201 ± 0.048
1.93AsnLys: 1.93 ± 0.032
5.904AsnLeu: 5.904 ± 0.078
0.62AsnMet: 0.62 ± 0.016
2.505AsnAsn: 2.505 ± 0.057
2.927AsnPro: 2.927 ± 0.042
2.84AsnGln: 2.84 ± 0.038
2.426AsnArg: 2.426 ± 0.035
3.445AsnSer: 3.445 ± 0.053
2.551AsnThr: 2.551 ± 0.049
2.444AsnVal: 2.444 ± 0.04
0.75AsnTrp: 0.75 ± 0.02
1.682AsnTyr: 1.682 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
3.092ProAla: 3.092 ± 0.04
0.336ProCys: 0.336 ± 0.014
2.833ProAsp: 2.833 ± 0.039
3.813ProGlu: 3.813 ± 0.049
1.71ProPhe: 1.71 ± 0.031
2.945ProGly: 2.945 ± 0.04
0.835ProHis: 0.835 ± 0.021
3.071ProIle: 3.071 ± 0.042
2.197ProLys: 2.197 ± 0.036
4.491ProLeu: 4.491 ± 0.05
0.671ProMet: 0.671 ± 0.018
2.369ProAsn: 2.369 ± 0.036
2.194ProPro: 2.194 ± 0.047
2.599ProGln: 2.599 ± 0.046
1.643ProArg: 1.643 ± 0.031
2.837ProSer: 2.837 ± 0.037
2.885ProThr: 2.885 ± 0.046
3.17ProVal: 3.17 ± 0.044
0.568ProTrp: 0.568 ± 0.018
1.29ProTyr: 1.29 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
4.765GlnAla: 4.765 ± 0.048
0.345GlnCys: 0.345 ± 0.014
2.076GlnAsp: 2.076 ± 0.035
3.728GlnGlu: 3.728 ± 0.043
1.724GlnPhe: 1.724 ± 0.03
3.254GlnGly: 3.254 ± 0.046
0.851GlnHis: 0.851 ± 0.022
3.861GlnIle: 3.861 ± 0.058
2.919GlnLys: 2.919 ± 0.045
6.129GlnLeu: 6.129 ± 0.074
0.997GlnMet: 0.997 ± 0.02
2.269GlnAsn: 2.269 ± 0.04
2.585GlnPro: 2.585 ± 0.036
3.897GlnGln: 3.897 ± 0.059
2.835GlnArg: 2.835 ± 0.038
2.911GlnSer: 2.911 ± 0.046
3.035GlnThr: 3.035 ± 0.04
3.685GlnVal: 3.685 ± 0.049
0.637GlnTrp: 0.637 ± 0.017
1.283GlnTyr: 1.283 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
3.099ArgAla: 3.099 ± 0.039
0.468ArgCys: 0.468 ± 0.015
2.465ArgAsp: 2.465 ± 0.039
3.298ArgGlu: 3.298 ± 0.042
2.195ArgPhe: 2.195 ± 0.035
2.903ArgGly: 2.903 ± 0.041
1.002ArgHis: 1.002 ± 0.024
3.285ArgIle: 3.285 ± 0.043
2.197ArgLys: 2.197 ± 0.039
5.886ArgLeu: 5.886 ± 0.055
0.933ArgMet: 0.933 ± 0.023
2.159ArgAsn: 2.159 ± 0.032
1.955ArgPro: 1.955 ± 0.036
3.277ArgGln: 3.277 ± 0.042
2.925ArgArg: 2.925 ± 0.042
3.18ArgSer: 3.18 ± 0.034
2.534ArgThr: 2.534 ± 0.037
3.35ArgVal: 3.35 ± 0.043
0.827ArgTrp: 0.827 ± 0.019
1.877ArgTyr: 1.877 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
4.545SerAla: 4.545 ± 0.051
0.595SerCys: 0.595 ± 0.017
3.33SerAsp: 3.33 ± 0.048
3.777SerGlu: 3.777 ± 0.043
2.688SerPhe: 2.688 ± 0.042
4.757SerGly: 4.757 ± 0.074
1.237SerHis: 1.237 ± 0.025
4.037SerIle: 4.037 ± 0.049
2.838SerLys: 2.838 ± 0.038
7.534SerLeu: 7.534 ± 0.069
1.075SerMet: 1.075 ± 0.026
3.084SerAsn: 3.084 ± 0.056
3.394SerPro: 3.394 ± 0.047
3.776SerGln: 3.776 ± 0.051
3.085SerArg: 3.085 ± 0.042
4.656SerSer: 4.656 ± 0.068
3.619SerThr: 3.619 ± 0.048
4.081SerVal: 4.081 ± 0.048
0.926SerTrp: 0.926 ± 0.021
1.966SerTyr: 1.966 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
4.779ThrAla: 4.779 ± 0.068
0.462ThrCys: 0.462 ± 0.015
2.715ThrAsp: 2.715 ± 0.047
3.274ThrGlu: 3.274 ± 0.039
2.286ThrPhe: 2.286 ± 0.036
4.361ThrGly: 4.361 ± 0.079
0.926ThrHis: 0.926 ± 0.022
3.945ThrIle: 3.945 ± 0.053
2.521ThrLys: 2.521 ± 0.039
6.33ThrLeu: 6.33 ± 0.069
0.707ThrMet: 0.707 ± 0.017
2.647ThrAsn: 2.647 ± 0.046
3.378ThrPro: 3.378 ± 0.047
3.043ThrGln: 3.043 ± 0.043
2.346ThrArg: 2.346 ± 0.033
3.712ThrSer: 3.712 ± 0.052
3.529ThrThr: 3.529 ± 0.055
3.997ThrVal: 3.997 ± 0.049
0.671ThrTrp: 0.671 ± 0.02
1.626ThrTyr: 1.626 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
5.773ValAla: 5.773 ± 0.06
0.663ValCys: 0.663 ± 0.019
3.316ValAsp: 3.316 ± 0.042
4.432ValGlu: 4.432 ± 0.051
2.591ValPhe: 2.591 ± 0.037
4.511ValGly: 4.511 ± 0.049
0.956ValHis: 0.956 ± 0.023
4.402ValIle: 4.402 ± 0.051
3.389ValLys: 3.389 ± 0.05
6.69ValLeu: 6.69 ± 0.058
1.254ValMet: 1.254 ± 0.025
3.077ValAsn: 3.077 ± 0.046
2.784ValPro: 2.784 ± 0.041
2.883ValGln: 2.883 ± 0.045
3.226ValArg: 3.226 ± 0.04
4.19ValSer: 4.19 ± 0.048
3.842ValThr: 3.842 ± 0.051
4.674ValVal: 4.674 ± 0.051
0.811ValTrp: 0.811 ± 0.022
1.823ValTyr: 1.823 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
0.865TrpAla: 0.865 ± 0.022
0.149TrpCys: 0.149 ± 0.009
0.618TrpAsp: 0.618 ± 0.022
0.913TrpGlu: 0.913 ± 0.026
0.592TrpPhe: 0.592 ± 0.017
0.95TrpGly: 0.95 ± 0.021
0.315TrpHis: 0.315 ± 0.013
0.842TrpIle: 0.842 ± 0.02
0.59TrpLys: 0.59 ± 0.018
1.761TrpLeu: 1.761 ± 0.036
0.284TrpMet: 0.284 ± 0.013
0.651TrpAsn: 0.651 ± 0.018
0.284TrpPro: 0.284 ± 0.013
1.152TrpGln: 1.152 ± 0.024
0.843TrpArg: 0.843 ± 0.022
0.806TrpSer: 0.806 ± 0.022
0.567TrpThr: 0.567 ± 0.017
0.92TrpVal: 0.92 ± 0.02
0.231TrpTrp: 0.231 ± 0.011
0.421TrpTyr: 0.421 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.097TyrAla: 2.097 ± 0.032
0.381TyrCys: 0.381 ± 0.015
1.474TyrAsp: 1.474 ± 0.028
1.754TyrGlu: 1.754 ± 0.032
1.428TyrPhe: 1.428 ± 0.032
2.052TyrGly: 2.052 ± 0.038
0.629TyrHis: 0.629 ± 0.017
1.856TyrIle: 1.856 ± 0.028
1.351TyrLys: 1.351 ± 0.026
3.621TyrLeu: 3.621 ± 0.043
0.421TyrMet: 0.421 ± 0.015
1.358TyrAsn: 1.358 ± 0.035
1.557TyrPro: 1.557 ± 0.026
2.017TyrGln: 2.017 ± 0.037
1.899TyrArg: 1.899 ± 0.032
2.048TyrSer: 2.048 ± 0.037
1.575TyrThr: 1.575 ± 0.03
1.624TyrVal: 1.624 ± 0.027
0.518TyrTrp: 0.518 ± 0.017
1.113TyrTyr: 1.113 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6881 proteins (2248921 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski