Elaphomyces granulatus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Elaphomycetaceae; Elaphomyces

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A232LSE7|A0A232LSE7_9EURO PKS_ER domain-containing protein OS=Elaphomyces granulatus OX=519963 GN=Egran_05180 PE=4 SV=1
MM1 pKa = 7.45QDD3 pKa = 2.7TVAIDD8 pKa = 3.54SVVSIRR14 pKa = 11.84KK15 pKa = 9.24FEE17 pKa = 4.19EE18 pKa = 3.57VTDD21 pKa = 3.75AHH23 pKa = 6.29KK24 pKa = 10.41HH25 pKa = 5.75LITEE29 pKa = 4.39AQYY32 pKa = 9.9TCAMGPNDD40 pKa = 3.57ATVTLGQCQSSDD52 pKa = 3.49MMNGDD57 pKa = 3.71QQSVTQAHH65 pKa = 5.65YY66 pKa = 10.86EE67 pKa = 4.01CDD69 pKa = 3.5LDD71 pKa = 5.7AFTAQNQLEE80 pKa = 4.24AVMDD84 pKa = 4.34ADD86 pKa = 5.13KK87 pKa = 11.57SLFTQDD93 pKa = 3.8QYY95 pKa = 12.02CGGATDD101 pKa = 4.03SAAVTQGQFEE111 pKa = 4.5AVMDD115 pKa = 4.4ADD117 pKa = 4.6KK118 pKa = 11.57SLFTQDD124 pKa = 3.8QYY126 pKa = 12.02CGGATDD132 pKa = 3.97SAAVTQSQFEE142 pKa = 4.08AVMDD146 pKa = 3.59VDD148 pKa = 4.35KK149 pKa = 11.41QLFTQGQYY157 pKa = 10.87CGGASVTQDD166 pKa = 2.63

Molecular weight: 17.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A232LYW8|A0A232LYW8_9EURO Uncharacterized protein OS=Elaphomyces granulatus OX=519963 GN=Egran_03008 PE=4 SV=1
MM1 pKa = 7.53SFRR4 pKa = 11.84GRR6 pKa = 11.84GRR8 pKa = 11.84GFATGANRR16 pKa = 11.84GGRR19 pKa = 11.84GGFQQQTFGPPAAVIGFYY37 pKa = 8.91QSRR40 pKa = 11.84SHH42 pKa = 6.23RR43 pKa = 11.84QVTSSPSPFLSALTKK58 pKa = 10.38IGTSKK63 pKa = 10.32PKK65 pKa = 10.13KK66 pKa = 10.02AGGATRR72 pKa = 11.84GGSRR76 pKa = 11.84GGGRR80 pKa = 11.84GAPRR84 pKa = 11.84GNFRR88 pKa = 11.84GGRR91 pKa = 11.84GGQRR95 pKa = 11.84GRR97 pKa = 11.84GGTRR101 pKa = 11.84GGPRR105 pKa = 11.84GGGRR109 pKa = 11.84GGSGGFSRR117 pKa = 11.84GGSRR121 pKa = 11.84GGFRR125 pKa = 11.84GGFRR129 pKa = 11.84GRR131 pKa = 11.84GG132 pKa = 3.28

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7163

6

7169

3610934

8

7225

503.7

55.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.817 ± 0.019

1.205 ± 0.01

5.759 ± 0.02

6.261 ± 0.03

3.747 ± 0.018

6.57 ± 0.025

2.41 ± 0.012

5.199 ± 0.019

4.834 ± 0.025

9.268 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.01

3.801 ± 0.013

6.023 ± 0.03

4.08 ± 0.019

6.504 ± 0.026

8.672 ± 0.034

5.737 ± 0.014

6.026 ± 0.02

1.345 ± 0.01

2.69 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski