Rhizobiales bacterium Sp-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Segnochrobactraceae; Segnochrobactrum

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6A7Y2Z1|A0A6A7Y2Z1_9RHIZ DUF1284 domain-containing protein OS=Rhizobiales bacterium Sp-1 OX=2608987 GN=F0357_05420 PE=4 SV=1
MM1 pKa = 7.95LDD3 pKa = 3.51LQGDD7 pKa = 4.01GTGYY11 pKa = 9.29ATITGNVVAVSSGSDD26 pKa = 3.25TLRR29 pKa = 11.84LSGLGGDD36 pKa = 4.56FDD38 pKa = 5.08GSLIDD43 pKa = 3.55TSGQFRR49 pKa = 11.84NFANMDD55 pKa = 3.17IDD57 pKa = 4.08TTGTWVLTTPQGGTSIGTVTVTNGTLKK84 pKa = 10.78LGNAQSDD91 pKa = 3.99GTLGSNVTVKK101 pKa = 10.74SGGTFANFSNGAGTTAANVSGSLTVEE127 pKa = 4.25SGGHH131 pKa = 4.86LQATVSNNYY140 pKa = 9.57AISAATVTLNAGAALDD156 pKa = 3.95VTLGAPQNSFALVYY170 pKa = 10.6SYY172 pKa = 11.9GNLALNGTVNITSNGAMGEE191 pKa = 4.28GTYY194 pKa = 11.29KK195 pKa = 10.71LIEE198 pKa = 4.19YY199 pKa = 9.97AGSLSGTTLVVGTAPSEE216 pKa = 3.53YY217 pKa = 10.22DD218 pKa = 3.46YY219 pKa = 11.87AVDD222 pKa = 3.67TTGTGYY228 pKa = 11.3VLLVVSAGGLYY239 pKa = 10.13WNGSTTTATGSVVGGSGTWTAAAGGVTNWTNSSGTTNVVTDD280 pKa = 3.8PTKK283 pKa = 9.59TAIFAGTAGTVTVDD297 pKa = 3.39ASSGAVSAKK306 pKa = 9.75GLKK309 pKa = 9.97FLTSGYY315 pKa = 9.4EE316 pKa = 3.66ITGATLTLANSSAAPIVNVDD336 pKa = 3.68GTSSLATISSVLAGSDD352 pKa = 3.42GLEE355 pKa = 4.33KK356 pKa = 10.43IGDD359 pKa = 3.85GTLVLSGVNTYY370 pKa = 9.72TGGTTVTAGTLQLGNATGVGSVAGGIVDD398 pKa = 3.73NAALVFDD405 pKa = 4.54NPTAQTYY412 pKa = 10.23GGVVSGTGTLEE423 pKa = 3.89KK424 pKa = 10.49QGAGILTLTGQNTFTGVTTITAGILQLGNGGITGTVSGNIVNNAALILNRR474 pKa = 11.84SGIGNDD480 pKa = 2.84AMAVNGAISGTGTIEE495 pKa = 3.7KK496 pKa = 10.1LGAGRR501 pKa = 11.84VTFNSSANTYY511 pKa = 10.66SGTTTISEE519 pKa = 4.22GTLEE523 pKa = 4.34IVNSTGLGSASAGTTVASGATLLVSSSGVLNEE555 pKa = 4.24PLTISGTGDD564 pKa = 3.19SVNFSSGNYY573 pKa = 8.01GAVSGGYY580 pKa = 7.82VTLGGTVTVAADD592 pKa = 3.33ATISVGGVTGEE603 pKa = 4.09VTGTGTTLTMIGGGIDD619 pKa = 4.11FSTTSTVSGSLNLVITGYY637 pKa = 9.95PSGGWPIVNFNGANTFTGTFTLEE660 pKa = 3.69SGLAFVNNSAALGSTEE676 pKa = 4.07GGTTIASGATLMLSPSSGSISIGAEE701 pKa = 3.36ALTISGTGYY710 pKa = 10.43FGFGALRR717 pKa = 11.84SAYY720 pKa = 10.08GNNSLAGPITLADD733 pKa = 4.02DD734 pKa = 3.48ATITVNSDD742 pKa = 3.04TLLLSGAIDD751 pKa = 4.15LSGKK755 pKa = 7.75TLTLTGSGGTISGTIAAGSGGVTVDD780 pKa = 5.0SSSSWIFTAANLYY793 pKa = 9.92TGDD796 pKa = 3.69TVIGSGATLQLGATGSAAGSVAGNIVDD823 pKa = 3.71NGSLSFVADD832 pKa = 3.41SNRR835 pKa = 11.84AYY837 pKa = 10.51AGVISGSGTLAKK849 pKa = 10.4SGTGTLTLTGNNTYY863 pKa = 9.71TGSTTVSGGLLQIGDD878 pKa = 4.11GGTTGWITSQVITNSGSDD896 pKa = 2.85SGGIVFNRR904 pKa = 11.84SDD906 pKa = 3.36NVTFSGTVGGSGTLEE921 pKa = 3.76QKK923 pKa = 11.11GPGTLTLTGSNMLSGTTTVSGGTLQLVGSLFGGPVDD959 pKa = 3.95VKK961 pKa = 11.25SGGTLLGTSTGMIFGNTTIEE981 pKa = 3.98NGGTLSAASGTYY993 pKa = 10.01FQMAGLSFGATSGLTVAVGAPSGTAAVYY1021 pKa = 10.58AGGALTLDD1029 pKa = 4.07GSLQLTTGAGFTSGTYY1045 pKa = 10.47RR1046 pKa = 11.84LIDD1049 pKa = 3.47YY1050 pKa = 8.53TGALTDD1056 pKa = 3.31NGMTVTSPVHH1066 pKa = 5.48SLYY1069 pKa = 10.87AIDD1072 pKa = 4.17TSTGGQVNLVVAVGQWWNGSNTTGGSQVVGGDD1104 pKa = 3.94GTWSVQAGATNWTNQAGSAASPWGQGGIAVFGGAAGTVTIAGATNPVVVGMDD1156 pKa = 3.99FVTDD1160 pKa = 4.81GYY1162 pKa = 9.4TVTGQSITLAPLTAGLTPAIMVDD1185 pKa = 3.04SGTATVASVLDD1196 pKa = 4.56GSAGMEE1202 pKa = 3.87KK1203 pKa = 9.61TGAGTLILAGTNTYY1217 pKa = 10.51SGQTTVSAGTLQIGDD1232 pKa = 3.82GGTTGSLSGNIVNNAALVFNRR1253 pKa = 11.84SDD1255 pKa = 3.31DD1256 pKa = 3.5VTYY1259 pKa = 11.1AGVISGAGTLEE1270 pKa = 3.94KK1271 pKa = 10.81LGGGTLTLTNNATTYY1286 pKa = 10.87SGLTTVSGGTLEE1298 pKa = 6.13LVGLWQRR1305 pKa = 11.84GGIALNGSTTRR1316 pKa = 11.84LLFNTSATTSVYY1328 pKa = 10.76SGLITGTGSVEE1339 pKa = 3.84KK1340 pKa = 9.9TGSNNVTLVADD1351 pKa = 3.59NTYY1354 pKa = 10.26TGGTTVTDD1362 pKa = 4.13GQLSIGNNTTTGSILGDD1379 pKa = 3.54VEE1381 pKa = 4.56VKK1383 pKa = 10.72AGAEE1387 pKa = 4.13LIISRR1392 pKa = 11.84SNTIVFGGNISGAGSVMVNGQGMAVLTGNVTNSGGVTVGIGSLQIGNGGTIGSVTGDD1449 pKa = 3.02IATDD1453 pKa = 3.88LLSFGGHH1460 pKa = 7.34LIFNRR1465 pKa = 11.84SDD1467 pKa = 3.26DD1468 pKa = 3.63LTYY1471 pKa = 11.16AGVISGAGTLAKK1483 pKa = 10.43LGAGTLTLTGEE1494 pKa = 4.31STLTGGTTITAGTLQIGNGGSTGSLAGAIVDD1525 pKa = 3.87NAALAFNRR1533 pKa = 11.84SDD1535 pKa = 4.09AITFAGVISGTGSLEE1550 pKa = 3.96QKK1552 pKa = 10.93GLGTLTLTGANTYY1565 pKa = 10.77SGGTVVSAGTLQLGDD1580 pKa = 3.61GGTTGSVSGAIANSGTVVFDD1600 pKa = 4.23RR1601 pKa = 11.84SDD1603 pKa = 3.43DD1604 pKa = 3.75VTASGSITGTGALVQAGTGTLTLVGSNSAGAGTTVSGGTLEE1645 pKa = 4.13ILSGVTLASDD1655 pKa = 3.44IVVQSGGTLQGEE1667 pKa = 4.63TSGTAGAAINGAVSVQDD1684 pKa = 3.43GGTLRR1689 pKa = 11.84AAPTSTAGVHH1699 pKa = 5.43GLSMTSLTLSNGANVDD1715 pKa = 3.95VILGSNTGIGVFSTGALTLDD1735 pKa = 3.37GVLNVTNAGAMSLGVYY1751 pKa = 10.23RR1752 pKa = 11.84LIDD1755 pKa = 3.89YY1756 pKa = 7.67TTMVANNGLVLGSTPTAFAYY1776 pKa = 8.84EE1777 pKa = 4.36VQQAPGQVNLAVLSGDD1793 pKa = 3.22MLYY1796 pKa = 10.47WNGSTTTPDD1805 pKa = 3.32GTIHH1809 pKa = 6.75GGNGTWTANAGQTNWLTSPLNQSRR1833 pKa = 11.84AWNSQFAVFAGTAGAVTVDD1852 pKa = 3.5NTAGPVSTTGMQFMVDD1868 pKa = 4.12GYY1870 pKa = 9.49SVSGGVVTLAASSGQTQVRR1889 pKa = 11.84VGDD1892 pKa = 3.95GTSAGAGYY1900 pKa = 10.05VATVGSVLDD1909 pKa = 3.84GTTGLEE1915 pKa = 4.17KK1916 pKa = 10.22TDD1918 pKa = 4.41LGTLILTGANTYY1930 pKa = 8.96TGNTTVTQGTLQIGNGGATGSIGGDD1955 pKa = 3.23VAVASGATLAFDD1967 pKa = 3.87RR1968 pKa = 11.84SGTVSFGGVISGAGGLTQAGPGTLTLTGTNTYY2000 pKa = 9.81TGGTTIAAGTLQIGNGGTTGSITGPVVNNGTLAFDD2035 pKa = 4.11RR2036 pKa = 11.84SDD2038 pKa = 3.24NTSFAEE2044 pKa = 4.83AISGSGSLIKK2054 pKa = 10.73NGSGTLTLAGTNAFTGSTTINGGTLSIMGGASLADD2089 pKa = 3.8GARR2092 pKa = 11.84LTVNANGLLNLVDD2105 pKa = 4.07ADD2107 pKa = 3.93EE2108 pKa = 4.87TVGSLAGGGNVALNTHH2124 pKa = 6.19CLITGGDD2131 pKa = 3.91GTSSTFSGGISGSGCVTKK2149 pKa = 10.41TGDD2152 pKa = 3.21GTMTLTGTSTYY2163 pKa = 10.99SGTTTVSGGAIAIGSADD2180 pKa = 4.25AIGTGPLALVGAGTLEE2196 pKa = 4.14AVDD2199 pKa = 4.92DD2200 pKa = 4.05FTFARR2205 pKa = 11.84GISLTPVDD2213 pKa = 4.18GSGGGTVTVDD2223 pKa = 2.93YY2224 pKa = 8.8TQTLTVSGVVSGNGALTKK2242 pKa = 10.26TGGGTLVLSGTNTFSGATTVDD2263 pKa = 3.45GGQLSLMGGSSLSDD2277 pKa = 3.14TARR2280 pKa = 11.84LTIAGFATVEE2290 pKa = 4.09LVDD2293 pKa = 3.89ADD2295 pKa = 3.93EE2296 pKa = 4.86TVGSLAGAGTVTLDD2310 pKa = 3.29GHH2312 pKa = 6.16CLSTGGDD2319 pKa = 3.73GTSSSFSGSLNGPGCLTKK2337 pKa = 10.54TGDD2340 pKa = 3.49GTLTLSGINTLTGPVTVSGGAVQVSAASALGGGPLALSGGGTLQASGTFTYY2391 pKa = 11.05ANAVSLTPVSGTGGGTFNVDD2411 pKa = 3.37DD2412 pKa = 4.93AQTLTLIGAITGLGNLAKK2430 pKa = 9.67TGTGTLVIEE2439 pKa = 4.84GANTASGATNVNAGTLVARR2458 pKa = 11.84GGQALSGEE2466 pKa = 4.54SAVTVASGAQLIVDD2480 pKa = 4.6GADD2483 pKa = 3.09ATIGSLSGAGAVQLSDD2499 pKa = 3.8ASLSLGGDD2507 pKa = 3.4NTTTTYY2513 pKa = 11.02SGTLSGTGGLTKK2525 pKa = 10.29TGTGTLTLAGANSYY2539 pKa = 10.74SGNTEE2544 pKa = 3.3IRR2546 pKa = 11.84GGALNVSGGLTDD2558 pKa = 3.43SDD2560 pKa = 4.54VYY2562 pKa = 11.58VYY2564 pKa = 10.83DD2565 pKa = 3.44QATLSGGGTITKK2577 pKa = 8.65TVHH2580 pKa = 5.36VLSGGTIAGSVGSGLTMGSLDD2601 pKa = 3.78MQAGATMSVSLGAPSGSGVFNINGNVNLAGTLAVNPNPGFGYY2643 pKa = 10.59GIYY2646 pKa = 10.3RR2647 pKa = 11.84IANYY2651 pKa = 10.66GGTLTDD2657 pKa = 2.95NGMTVSGLPSNLEE2670 pKa = 4.01GGLQTAIPGQVNLYY2684 pKa = 10.51VDD2686 pKa = 4.65DD2687 pKa = 5.09PNNPTEE2693 pKa = 4.23FWNGANTTPTQAVLGGTGTWTAGAQTNWVNEE2724 pKa = 4.18SGTVSKK2730 pKa = 8.91TWNSGFAVFQGSAGTVTVDD2749 pKa = 3.47DD2750 pKa = 3.78TDD2752 pKa = 3.87VAVSAVGMQFVTSGYY2767 pKa = 10.38VVTGDD2772 pKa = 4.42SINLTGTALIRR2783 pKa = 11.84VGDD2786 pKa = 3.9GTLAGAATTATIASVLTGTSGLEE2809 pKa = 3.71KK2810 pKa = 10.13SDD2812 pKa = 3.56YY2813 pKa = 9.06GTLVLSGANTYY2824 pKa = 10.94SGGTRR2829 pKa = 11.84LTAGTLAVGNNGALGTGTLAMAEE2852 pKa = 4.26GTTLAFATDD2861 pKa = 3.99GLTLANAVTLTGDD2874 pKa = 3.37PTISVGSGQTDD2885 pKa = 3.37TLAGVLSDD2893 pKa = 3.67GTQPGDD2899 pKa = 3.17IVKK2902 pKa = 9.26TGGGTLVLTGNNTYY2916 pKa = 10.89SGGTTISEE2924 pKa = 4.1GTLQIGNGGTTGSIVGDD2941 pKa = 3.91VIDD2944 pKa = 4.34NATLTFDD2951 pKa = 4.15RR2952 pKa = 11.84SDD2954 pKa = 3.46DD2955 pKa = 3.6VTFAGAISGSGALVQSGTGTLMLTGANTYY2984 pKa = 9.59TGGTTVSAGTLAGNATSLQGNIVDD3008 pKa = 3.83NAAVVFNQTTTGTYY3022 pKa = 10.25AGAISGTGSMTKK3034 pKa = 9.6TGTGTLILIGEE3045 pKa = 4.17NTYY3048 pKa = 10.31TGGTTISEE3056 pKa = 4.16GTLQIGNGGTTGSLVGAIVNNAALVFNRR3084 pKa = 11.84SDD3086 pKa = 3.35TYY3088 pKa = 11.41NFPGTISGPGSVTIIGGTVNFTGANAYY3115 pKa = 9.96SGPIAVADD3123 pKa = 3.81ATFEE3127 pKa = 4.17LAPGAVSASSYY3138 pKa = 10.26TIGAGGVIGGTGTIAGLSVLNGGTAAPGYY3167 pKa = 10.49SPGTLTVNGNVSFGAGSVYY3186 pKa = 10.48QVDD3189 pKa = 3.53ITPQGAHH3196 pKa = 6.9DD3197 pKa = 5.38LIIASGTATISGGTVQVNAAQGTYY3221 pKa = 10.45VPGSTYY3227 pKa = 10.49TILTAQGGVSGQFSGLTVNYY3247 pKa = 10.32AYY3249 pKa = 10.82LDD3251 pKa = 3.75PVLSYY3256 pKa = 10.89DD3257 pKa = 4.0ANDD3260 pKa = 3.15VYY3262 pKa = 10.85LTVWRR3267 pKa = 11.84NTVSFPDD3274 pKa = 3.32AAYY3277 pKa = 7.08TTNQKK3282 pKa = 9.26VAAAAVEE3289 pKa = 4.1NLPITNPVYY3298 pKa = 9.74TAVVQLSGAEE3308 pKa = 3.74APAAFDD3314 pKa = 3.87ALSGEE3319 pKa = 4.82AYY3321 pKa = 10.29ASASSVMQQQSSYY3334 pKa = 10.66LRR3336 pKa = 11.84EE3337 pKa = 4.03AVGTRR3342 pKa = 11.84VRR3344 pKa = 11.84QGLIGQSGFGQEE3356 pKa = 4.14TAKK3359 pKa = 10.7LAPGYY3364 pKa = 10.11DD3365 pKa = 3.21ATVWTQAYY3373 pKa = 7.75GAWGLTAGNGNAASVDD3389 pKa = 3.6RR3390 pKa = 11.84TIGGFFTGIDD3400 pKa = 3.43AALSEE3405 pKa = 4.32AARR3408 pKa = 11.84FGIVGGYY3415 pKa = 9.01SRR3417 pKa = 11.84STFNVDD3423 pKa = 2.81ARR3425 pKa = 11.84SSSGDD3430 pKa = 2.87IDD3432 pKa = 4.76NYY3434 pKa = 11.62DD3435 pKa = 3.41LGLYY3439 pKa = 10.2GGAKK3443 pKa = 9.98FGSLSFLAAASYY3455 pKa = 8.35TWHH3458 pKa = 7.29DD3459 pKa = 3.76LSVDD3463 pKa = 3.38RR3464 pKa = 11.84TIAFSGFSGIASADD3478 pKa = 3.6YY3479 pKa = 10.63KK3480 pKa = 11.29AGTTQVFSEE3489 pKa = 4.3AAWRR3493 pKa = 11.84VDD3495 pKa = 3.34LSKK3498 pKa = 11.26SVDD3501 pKa = 3.08AKK3503 pKa = 10.42TFGAASIEE3511 pKa = 4.17PFANLAYY3518 pKa = 10.97VNLSSQNFTEE3528 pKa = 4.32TGTPAALTGSAEE3540 pKa = 4.37TEE3542 pKa = 3.77NTLYY3546 pKa = 9.94STLGVRR3552 pKa = 11.84AATVFEE3558 pKa = 4.51LSNGASLTPHH3568 pKa = 7.26ASLGWQHH3575 pKa = 6.82AFGDD3579 pKa = 4.47VNSSASLAFASGGSAFSVAGVPIARR3604 pKa = 11.84DD3605 pKa = 3.28AALVGAGVDD3614 pKa = 3.62YY3615 pKa = 11.19AFSKK3619 pKa = 10.73SVSASVTYY3627 pKa = 10.44SGQFGSGTEE3636 pKa = 3.93DD3637 pKa = 3.06NAFKK3641 pKa = 10.14GTINIKK3647 pKa = 10.14FF3648 pKa = 3.45

Molecular weight:
353.45 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6A7Y718|A0A6A7Y718_9RHIZ Type VI secretion system contractile sheath small subunit OS=Rhizobiales bacterium Sp-1 OX=2608987 GN=tssB PE=4 SV=1
MM1 pKa = 7.45WPLRR5 pKa = 11.84SPSPRR10 pKa = 11.84PRR12 pKa = 11.84PPTTAPSCPRR22 pKa = 11.84RR23 pKa = 11.84SRR25 pKa = 11.84PIRR28 pKa = 11.84PPRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84WRR35 pKa = 11.84PSRR38 pKa = 11.84RR39 pKa = 11.84PAGSPRR45 pKa = 11.84CSAARR50 pKa = 11.84SPPPPRR56 pKa = 11.84RR57 pKa = 11.84WPPRR61 pKa = 11.84SRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84PRR67 pKa = 11.84RR68 pKa = 11.84CARR71 pKa = 11.84SRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84PRR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 3.13

Molecular weight:
9.71 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4553

0

4553

1515680

18

6203

332.9

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.081 ± 0.064

0.722 ± 0.01

5.655 ± 0.03

5.293 ± 0.043

3.611 ± 0.022

9.133 ± 0.076

1.938 ± 0.021

5.035 ± 0.031

2.671 ± 0.029

10.122 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.058 ± 0.018

2.263 ± 0.027

5.499 ± 0.04

2.502 ± 0.024

7.391 ± 0.059

5.199 ± 0.05

5.593 ± 0.071

7.894 ± 0.031

1.265 ± 0.017

2.075 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski