Amino acid dipepetide frequency for Rhizobiales bacterium Sp-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.748AlaAla: 22.748 ± 0.206
0.977AlaCys: 0.977 ± 0.026
7.916AlaAsp: 7.916 ± 0.085
8.008AlaGlu: 8.008 ± 0.116
5.072AlaPhe: 5.072 ± 0.062
12.668AlaGly: 12.668 ± 0.125
2.409AlaHis: 2.409 ± 0.041
7.115AlaIle: 7.115 ± 0.074
3.672AlaLys: 3.672 ± 0.057
15.078AlaLeu: 15.078 ± 0.128
3.267AlaMet: 3.267 ± 0.052
2.779AlaAsn: 2.779 ± 0.06
6.896AlaPro: 6.896 ± 0.103
3.324AlaGln: 3.324 ± 0.057
10.275AlaArg: 10.275 ± 0.128
6.776AlaSer: 6.776 ± 0.092
7.097AlaThr: 7.097 ± 0.087
10.574AlaVal: 10.574 ± 0.094
1.574AlaTrp: 1.574 ± 0.034
2.582AlaTyr: 2.582 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.863CysAla: 0.863 ± 0.024
0.082CysCys: 0.082 ± 0.007
0.46CysAsp: 0.46 ± 0.019
0.36CysGlu: 0.36 ± 0.015
0.266CysPhe: 0.266 ± 0.012
0.848CysGly: 0.848 ± 0.025
0.209CysHis: 0.209 ± 0.014
0.298CysIle: 0.298 ± 0.013
0.151CysLys: 0.151 ± 0.011
0.699CysLeu: 0.699 ± 0.024
0.127CysMet: 0.127 ± 0.009
0.172CysAsn: 0.172 ± 0.01
0.408CysPro: 0.408 ± 0.018
0.144CysGln: 0.144 ± 0.011
0.617CysArg: 0.617 ± 0.022
0.358CysSer: 0.358 ± 0.015
0.364CysThr: 0.364 ± 0.016
0.546CysVal: 0.546 ± 0.02
0.095CysTrp: 0.095 ± 0.008
0.154CysTyr: 0.154 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.432AspAla: 7.432 ± 0.082
0.381AspCys: 0.381 ± 0.015
3.105AspAsp: 3.105 ± 0.053
3.327AspGlu: 3.327 ± 0.059
2.016AspPhe: 2.016 ± 0.041
5.7AspGly: 5.7 ± 0.074
1.273AspHis: 1.273 ± 0.037
2.714AspIle: 2.714 ± 0.043
1.355AspLys: 1.355 ± 0.031
6.437AspLeu: 6.437 ± 0.074
1.01AspMet: 1.01 ± 0.028
1.042AspAsn: 1.042 ± 0.032
3.866AspPro: 3.866 ± 0.06
1.455AspGln: 1.455 ± 0.039
4.553AspArg: 4.553 ± 0.076
1.919AspSer: 1.919 ± 0.039
2.38AspThr: 2.38 ± 0.04
4.285AspVal: 4.285 ± 0.064
0.897AspTrp: 0.897 ± 0.022
1.401AspTyr: 1.401 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
8.464GluAla: 8.464 ± 0.117
0.321GluCys: 0.321 ± 0.014
2.476GluAsp: 2.476 ± 0.043
2.736GluGlu: 2.736 ± 0.051
1.445GluPhe: 1.445 ± 0.03
4.192GluGly: 4.192 ± 0.059
1.122GluHis: 1.122 ± 0.027
3.637GluIle: 3.637 ± 0.059
1.606GluLys: 1.606 ± 0.04
4.296GluLeu: 4.296 ± 0.058
1.164GluMet: 1.164 ± 0.025
1.189GluAsn: 1.189 ± 0.028
2.96GluPro: 2.96 ± 0.059
1.463GluGln: 1.463 ± 0.031
5.115GluArg: 5.115 ± 0.081
2.101GluSer: 2.101 ± 0.041
3.621GluThr: 3.621 ± 0.056
3.731GluVal: 3.731 ± 0.061
0.537GluTrp: 0.537 ± 0.022
0.752GluTyr: 0.752 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
5.002PheAla: 5.002 ± 0.057
0.351PheCys: 0.351 ± 0.015
2.505PheAsp: 2.505 ± 0.044
2.072PheGlu: 2.072 ± 0.043
1.257PhePhe: 1.257 ± 0.033
3.728PheGly: 3.728 ± 0.058
0.726PheHis: 0.726 ± 0.023
1.409PheIle: 1.409 ± 0.03
0.846PheLys: 0.846 ± 0.026
3.377PheLeu: 3.377 ± 0.058
0.657PheMet: 0.657 ± 0.022
0.932PheAsn: 0.932 ± 0.031
1.645PhePro: 1.645 ± 0.034
0.914PheGln: 0.914 ± 0.024
2.256PheArg: 2.256 ± 0.043
1.997PheSer: 1.997 ± 0.043
1.969PheThr: 1.969 ± 0.043
3.148PheVal: 3.148 ± 0.053
0.501PheTrp: 0.501 ± 0.021
0.817PheTyr: 0.817 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
11.12GlyAla: 11.12 ± 0.116
0.755GlyCys: 0.755 ± 0.023
4.657GlyAsp: 4.657 ± 0.057
4.627GlyGlu: 4.627 ± 0.069
3.617GlyPhe: 3.617 ± 0.064
8.852GlyGly: 8.852 ± 0.287
1.924GlyHis: 1.924 ± 0.041
4.621GlyIle: 4.621 ± 0.056
2.632GlyLys: 2.632 ± 0.054
9.91GlyLeu: 9.91 ± 0.097
1.927GlyMet: 1.927 ± 0.036
1.978GlyAsn: 1.978 ± 0.07
4.09GlyPro: 4.09 ± 0.057
2.361GlyGln: 2.361 ± 0.045
7.321GlyArg: 7.321 ± 0.087
4.948GlySer: 4.948 ± 0.134
5.968GlyThr: 5.968 ± 0.313
6.424GlyVal: 6.424 ± 0.071
1.396GlyTrp: 1.396 ± 0.036
2.198GlyTyr: 2.198 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.439HisAla: 2.439 ± 0.045
0.195HisCys: 0.195 ± 0.011
1.209HisAsp: 1.209 ± 0.036
0.974HisGlu: 0.974 ± 0.025
0.809HisPhe: 0.809 ± 0.025
1.972HisGly: 1.972 ± 0.04
0.554HisHis: 0.554 ± 0.024
0.829HisIle: 0.829 ± 0.021
0.412HisLys: 0.412 ± 0.016
2.042HisLeu: 2.042 ± 0.041
0.399HisMet: 0.399 ± 0.017
0.364HisAsn: 0.364 ± 0.015
1.398HisPro: 1.398 ± 0.033
0.473HisGln: 0.473 ± 0.02
1.519HisArg: 1.519 ± 0.035
0.761HisSer: 0.761 ± 0.025
0.784HisThr: 0.784 ± 0.022
1.406HisVal: 1.406 ± 0.036
0.315HisTrp: 0.315 ± 0.016
0.521HisTyr: 0.521 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
8.208IleAla: 8.208 ± 0.083
0.397IleCys: 0.397 ± 0.017
3.519IleAsp: 3.519 ± 0.047
3.531IleGlu: 3.531 ± 0.054
1.455IlePhe: 1.455 ± 0.034
5.403IleGly: 5.403 ± 0.068
0.831IleHis: 0.831 ± 0.021
1.799IleIle: 1.799 ± 0.042
1.083IleLys: 1.083 ± 0.03
4.362IleLeu: 4.362 ± 0.061
0.706IleMet: 0.706 ± 0.022
1.199IleAsn: 1.199 ± 0.031
2.228IlePro: 2.228 ± 0.04
1.006IleGln: 1.006 ± 0.025
3.164IleArg: 3.164 ± 0.055
2.572IleSer: 2.572 ± 0.064
2.481IleThr: 2.481 ± 0.049
4.88IleVal: 4.88 ± 0.057
0.514IleTrp: 0.514 ± 0.018
1.005IleTyr: 1.005 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
4.0LysAla: 4.0 ± 0.067
0.1LysCys: 0.1 ± 0.008
1.379LysAsp: 1.379 ± 0.036
1.135LysGlu: 1.135 ± 0.029
0.709LysPhe: 0.709 ± 0.025
2.21LysGly: 2.21 ± 0.043
0.436LysHis: 0.436 ± 0.017
1.5LysIle: 1.5 ± 0.044
0.839LysLys: 0.839 ± 0.026
2.58LysLeu: 2.58 ± 0.045
0.548LysMet: 0.548 ± 0.019
0.614LysAsn: 0.614 ± 0.023
1.848LysPro: 1.848 ± 0.042
0.658LysGln: 0.658 ± 0.022
1.931LysArg: 1.931 ± 0.038
1.474LysSer: 1.474 ± 0.032
1.833LysThr: 1.833 ± 0.042
2.193LysVal: 2.193 ± 0.046
0.275LysTrp: 0.275 ± 0.013
0.453LysTyr: 0.453 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
15.508LeuAla: 15.508 ± 0.129
0.756LeuCys: 0.756 ± 0.022
6.429LeuAsp: 6.429 ± 0.08
4.908LeuGlu: 4.908 ± 0.075
3.47LeuPhe: 3.47 ± 0.056
8.932LeuGly: 8.932 ± 0.085
1.756LeuHis: 1.756 ± 0.036
4.857LeuIle: 4.857 ± 0.061
3.335LeuLys: 3.335 ± 0.057
8.669LeuLeu: 8.669 ± 0.107
2.092LeuMet: 2.092 ± 0.038
2.239LeuAsn: 2.239 ± 0.038
5.57LeuPro: 5.57 ± 0.073
2.331LeuGln: 2.331 ± 0.051
6.441LeuArg: 6.441 ± 0.078
6.328LeuSer: 6.328 ± 0.086
5.623LeuThr: 5.623 ± 0.134
8.663LeuVal: 8.663 ± 0.096
1.155LeuTrp: 1.155 ± 0.035
2.159LeuTyr: 2.159 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
2.603MetAla: 2.603 ± 0.046
0.123MetCys: 0.123 ± 0.009
0.848MetAsp: 0.848 ± 0.026
0.854MetGlu: 0.854 ± 0.021
0.587MetPhe: 0.587 ± 0.021
1.442MetGly: 1.442 ± 0.029
0.317MetHis: 0.317 ± 0.014
1.183MetIle: 1.183 ± 0.031
0.739MetLys: 0.739 ± 0.023
2.066MetLeu: 2.066 ± 0.041
0.521MetMet: 0.521 ± 0.021
0.612MetAsn: 0.612 ± 0.02
1.364MetPro: 1.364 ± 0.032
0.567MetGln: 0.567 ± 0.017
1.586MetArg: 1.586 ± 0.039
1.5MetSer: 1.5 ± 0.03
1.681MetThr: 1.681 ± 0.032
1.569MetVal: 1.569 ± 0.034
0.199MetTrp: 0.199 ± 0.011
0.218MetTyr: 0.218 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
3.125AsnAla: 3.125 ± 0.058
0.174AsnCys: 0.174 ± 0.011
1.151AsnAsp: 1.151 ± 0.025
0.955AsnGlu: 0.955 ± 0.025
0.798AsnPhe: 0.798 ± 0.029
2.365AsnGly: 2.365 ± 0.066
0.409AsnHis: 0.409 ± 0.018
1.103AsnIle: 1.103 ± 0.029
0.497AsnLys: 0.497 ± 0.022
2.27AsnLeu: 2.27 ± 0.044
0.45AsnMet: 0.45 ± 0.017
0.599AsnAsn: 0.599 ± 0.031
1.61AsnPro: 1.61 ± 0.034
0.606AsnGln: 0.606 ± 0.021
1.541AsnArg: 1.541 ± 0.034
0.963AsnSer: 0.963 ± 0.036
1.215AsnThr: 1.215 ± 0.059
1.883AsnVal: 1.883 ± 0.042
0.341AsnTrp: 0.341 ± 0.015
0.583AsnTyr: 0.583 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
7.8ProAla: 7.8 ± 0.11
0.3ProCys: 0.3 ± 0.015
3.724ProAsp: 3.724 ± 0.057
3.348ProGlu: 3.348 ± 0.051
2.205ProPhe: 2.205 ± 0.037
4.759ProGly: 4.759 ± 0.068
1.118ProHis: 1.118 ± 0.031
2.65ProIle: 2.65 ± 0.049
1.507ProLys: 1.507 ± 0.036
5.064ProLeu: 5.064 ± 0.066
1.071ProMet: 1.071 ± 0.027
1.38ProAsn: 1.38 ± 0.033
3.032ProPro: 3.032 ± 0.075
1.377ProGln: 1.377 ± 0.04
3.379ProArg: 3.379 ± 0.066
3.024ProSer: 3.024 ± 0.049
2.918ProThr: 2.918 ± 0.048
4.436ProVal: 4.436 ± 0.071
0.763ProTrp: 0.763 ± 0.024
1.139ProTyr: 1.139 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.536GlnAla: 3.536 ± 0.053
0.158GlnCys: 0.158 ± 0.01
1.156GlnAsp: 1.156 ± 0.03
1.108GlnGlu: 1.108 ± 0.028
0.835GlnPhe: 0.835 ± 0.025
2.064GlnGly: 2.064 ± 0.037
0.428GlnHis: 0.428 ± 0.018
1.605GlnIle: 1.605 ± 0.035
0.758GlnLys: 0.758 ± 0.027
2.14GlnLeu: 2.14 ± 0.04
0.637GlnMet: 0.637 ± 0.021
0.713GlnAsn: 0.713 ± 0.026
1.417GlnPro: 1.417 ± 0.035
0.867GlnGln: 0.867 ± 0.029
1.927GlnArg: 1.927 ± 0.042
1.552GlnSer: 1.552 ± 0.043
1.49GlnThr: 1.49 ± 0.035
1.876GlnVal: 1.876 ± 0.044
0.297GlnTrp: 0.297 ± 0.015
0.462GlnTyr: 0.462 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
9.377ArgAla: 9.377 ± 0.12
0.49ArgCys: 0.49 ± 0.019
4.112ArgAsp: 4.112 ± 0.067
3.858ArgGlu: 3.858 ± 0.066
3.034ArgPhe: 3.034 ± 0.049
5.268ArgGly: 5.268 ± 0.08
1.721ArgHis: 1.721 ± 0.039
4.016ArgIle: 4.016 ± 0.06
1.766ArgLys: 1.766 ± 0.035
8.66ArgLeu: 8.66 ± 0.101
1.674ArgMet: 1.674 ± 0.036
1.506ArgAsn: 1.506 ± 0.032
4.244ArgPro: 4.244 ± 0.071
2.063ArgGln: 2.063 ± 0.042
7.011ArgArg: 7.011 ± 0.101
3.678ArgSer: 3.678 ± 0.05
3.81ArgThr: 3.81 ± 0.057
5.085ArgVal: 5.085 ± 0.071
0.971ArgTrp: 0.971 ± 0.029
1.562ArgTyr: 1.562 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.574SerAla: 6.574 ± 0.09
0.343SerCys: 0.343 ± 0.017
2.79SerAsp: 2.79 ± 0.045
2.433SerGlu: 2.433 ± 0.045
2.095SerPhe: 2.095 ± 0.032
5.926SerGly: 5.926 ± 0.225
1.013SerHis: 1.013 ± 0.028
2.591SerIle: 2.591 ± 0.053
1.23SerLys: 1.23 ± 0.031
5.487SerLeu: 5.487 ± 0.068
1.019SerMet: 1.019 ± 0.025
1.242SerAsn: 1.242 ± 0.035
2.961SerPro: 2.961 ± 0.041
1.262SerGln: 1.262 ± 0.031
3.485SerArg: 3.485 ± 0.056
2.813SerSer: 2.813 ± 0.08
2.782SerThr: 2.782 ± 0.064
4.16SerVal: 4.16 ± 0.07
0.662SerTrp: 0.662 ± 0.022
1.125SerTyr: 1.125 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
7.184ThrAla: 7.184 ± 0.087
0.366ThrCys: 0.366 ± 0.016
2.837ThrAsp: 2.837 ± 0.045
2.583ThrGlu: 2.583 ± 0.046
2.218ThrPhe: 2.218 ± 0.049
5.538ThrGly: 5.538 ± 0.168
0.986ThrHis: 0.986 ± 0.026
3.158ThrIle: 3.158 ± 0.072
1.31ThrLys: 1.31 ± 0.033
6.501ThrLeu: 6.501 ± 0.183
1.05ThrMet: 1.05 ± 0.026
1.318ThrAsn: 1.318 ± 0.048
3.41ThrPro: 3.41 ± 0.056
1.311ThrGln: 1.311 ± 0.041
3.393ThrArg: 3.393 ± 0.052
2.853ThrSer: 2.853 ± 0.07
3.222ThrThr: 3.222 ± 0.102
4.637ThrVal: 4.637 ± 0.083
0.709ThrTrp: 0.709 ± 0.021
1.344ThrTyr: 1.344 ± 0.065
0.0ThrXaa: 0.0 ± 0.0
Val
11.054ValAla: 11.054 ± 0.103
0.635ValCys: 0.635 ± 0.022
4.292ValAsp: 4.292 ± 0.05
4.502ValGlu: 4.502 ± 0.065
2.947ValPhe: 2.947 ± 0.05
6.423ValGly: 6.423 ± 0.08
1.418ValHis: 1.418 ± 0.033
3.835ValIle: 3.835 ± 0.059
2.08ValLys: 2.08 ± 0.041
8.055ValLeu: 8.055 ± 0.08
1.627ValMet: 1.627 ± 0.036
1.834ValAsn: 1.834 ± 0.047
4.362ValPro: 4.362 ± 0.062
1.765ValGln: 1.765 ± 0.037
5.475ValArg: 5.475 ± 0.074
4.476ValSer: 4.476 ± 0.076
4.849ValThr: 4.849 ± 0.077
6.951ValVal: 6.951 ± 0.075
0.874ValTrp: 0.874 ± 0.021
1.482ValTyr: 1.482 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.204TrpAla: 1.204 ± 0.033
0.117TrpCys: 0.117 ± 0.009
0.616TrpAsp: 0.616 ± 0.022
0.488TrpGlu: 0.488 ± 0.02
0.496TrpPhe: 0.496 ± 0.018
0.924TrpGly: 0.924 ± 0.026
0.33TrpHis: 0.33 ± 0.014
0.659TrpIle: 0.659 ± 0.024
0.373TrpLys: 0.373 ± 0.016
1.508TrpLeu: 1.508 ± 0.034
0.288TrpMet: 0.288 ± 0.014
0.402TrpAsn: 0.402 ± 0.017
0.693TrpPro: 0.693 ± 0.023
0.457TrpGln: 0.457 ± 0.017
1.217TrpArg: 1.217 ± 0.028
0.802TrpSer: 0.802 ± 0.022
0.794TrpThr: 0.794 ± 0.025
0.792TrpVal: 0.792 ± 0.024
0.226TrpTrp: 0.226 ± 0.013
0.268TrpTyr: 0.268 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.567TyrAla: 2.567 ± 0.051
0.2TyrCys: 0.2 ± 0.012
1.367TyrAsp: 1.367 ± 0.033
1.121TyrGlu: 1.121 ± 0.03
0.78TyrPhe: 0.78 ± 0.024
2.111TyrGly: 2.111 ± 0.044
0.395TyrHis: 0.395 ± 0.016
0.768TyrIle: 0.768 ± 0.024
0.52TyrLys: 0.52 ± 0.021
2.02TyrLeu: 2.02 ± 0.04
0.346TyrMet: 0.346 ± 0.015
0.524TyrAsn: 0.524 ± 0.02
0.992TyrPro: 0.992 ± 0.026
0.62TyrGln: 0.62 ± 0.021
1.7TyrArg: 1.7 ± 0.035
1.097TyrSer: 1.097 ± 0.035
1.046TyrThr: 1.046 ± 0.039
1.697TyrVal: 1.697 ± 0.034
0.354TyrTrp: 0.354 ± 0.015
0.521TyrTyr: 0.521 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4553 proteins (1515680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski