Rosellinia necatrix (White root-rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariaceae; Rosellinia

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12440 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W2TW94|A0A1W2TW94_ROSNE Putative WD repeat-containing protein OS=Rosellinia necatrix OX=77044 GN=SAMD00023353_8400150 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.23SFAVITGLLAVGVLSAPVKK21 pKa = 10.06PVSEE25 pKa = 4.29LDD27 pKa = 3.44SFNYY31 pKa = 8.79TNNIDD36 pKa = 3.99NAVTASDD43 pKa = 3.59AVSDD47 pKa = 3.59EE48 pKa = 4.33FNYY51 pKa = 10.52HH52 pKa = 5.82NNIDD56 pKa = 3.96NAVTANDD63 pKa = 3.94AEE65 pKa = 4.36SDD67 pKa = 3.45AFNYY71 pKa = 10.28SNNIDD76 pKa = 3.57NAVNAKK82 pKa = 9.76DD83 pKa = 3.72VNEE86 pKa = 4.55DD87 pKa = 3.45SFNYY91 pKa = 8.87TNNIDD96 pKa = 3.75NAVNANDD103 pKa = 4.45AKK105 pKa = 10.84SDD107 pKa = 3.14AYY109 pKa = 10.98NYY111 pKa = 10.33HH112 pKa = 6.39NNIDD116 pKa = 3.77NAVTVTDD123 pKa = 3.83ADD125 pKa = 3.83GTKK128 pKa = 10.53VILSSPYY135 pKa = 9.63TDD137 pKa = 4.52DD138 pKa = 4.49VNAADD143 pKa = 3.84AASILGEE150 pKa = 4.01

Molecular weight: 15.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S8A9A8|A0A1S8A9A8_ROSNE Putative HET domain-containing protein OS=Rosellinia necatrix OX=77044 GN=SAMD00023353_4400630 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12440

0

12440

5726925

49

6519

460.4

50.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.245 ± 0.024

1.197 ± 0.009

5.891 ± 0.015

5.916 ± 0.02

3.576 ± 0.012

7.309 ± 0.024

2.419 ± 0.01

4.786 ± 0.015

4.371 ± 0.018

8.81 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.07 ± 0.007

3.588 ± 0.014

6.203 ± 0.026

3.87 ± 0.015

6.497 ± 0.02

7.975 ± 0.023

5.956 ± 0.016

6.127 ± 0.015

1.469 ± 0.008

2.725 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski