Enterococcus thailandicus
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2368 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A179EUW3|A0A179EUW3_ENTTH Phosphopantothenoylcysteine decarboxylase OS=Enterococcus thailandicus OX=417368 GN=coaC PE=4 SV=1
MM1 pKa = 7.16 TLAKK5 pKa = 9.51 IVYY8 pKa = 10.46 ASMTGNTEE16 pKa = 3.89 EE17 pKa = 4.35 IADD20 pKa = 3.64 IVAEE24 pKa = 4.08 AFEE27 pKa = 4.39 NLDD30 pKa = 2.92 IDD32 pKa = 4.72 VEE34 pKa = 4.5 INEE37 pKa = 4.52 CTQVDD42 pKa = 3.42 ADD44 pKa = 4.07 EE45 pKa = 5.83 FEE47 pKa = 4.93 DD48 pKa = 5.21 ADD50 pKa = 3.59 ICVVATYY57 pKa = 9.24 TYY59 pKa = 11.39 GDD61 pKa = 3.83 GEE63 pKa = 4.56 LPDD66 pKa = 4.96 EE67 pKa = 4.49 IVDD70 pKa = 4.49 FYY72 pKa = 11.82 EE73 pKa = 4.8 DD74 pKa = 3.59 LQEE77 pKa = 4.21 VDD79 pKa = 5.18 LEE81 pKa = 4.28 GKK83 pKa = 10.11 IYY85 pKa = 9.85 GVCGSGDD92 pKa = 3.21 TFYY95 pKa = 11.45 DD96 pKa = 4.02 EE97 pKa = 4.62 FCKK100 pKa = 10.96 SVDD103 pKa = 3.59 DD104 pKa = 4.43 FDD106 pKa = 5.88 AVFAKK111 pKa = 10.19 TGATKK116 pKa = 10.29 GAEE119 pKa = 4.01 NVKK122 pKa = 10.18 VDD124 pKa = 4.48 LNAEE128 pKa = 4.09 EE129 pKa = 4.29 EE130 pKa = 4.49 DD131 pKa = 3.88 IEE133 pKa = 4.32 NLEE136 pKa = 4.33 AFAKK140 pKa = 10.81 ALVAKK145 pKa = 10.53 AKK147 pKa = 10.48
Molecular weight: 16.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.605
IPC_protein 3.592
Toseland 3.389
ProMoST 3.745
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.49
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.516
Nozaki 3.694
DTASelect 3.884
Thurlkill 3.439
EMBOSS 3.503
Sillero 3.706
Patrickios 1.799
IPC_peptide 3.554
IPC2_peptide 3.681
IPC2.peptide.svr19 3.687
Protein with the highest isoelectric point:
>tr|A0A179ERJ8|A0A179ERJ8_ENTTH Chromosomal replication initiator protein DnaA OS=Enterococcus thailandicus OX=417368 GN=dnaA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 9.16 RR10 pKa = 11.84 KK11 pKa = 9.12 RR12 pKa = 11.84 QKK14 pKa = 9.38 VHH16 pKa = 5.8 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2368
0
2368
739427
38
1872
312.3
35.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.153 ± 0.052
0.553 ± 0.012
5.103 ± 0.039
7.616 ± 0.054
4.719 ± 0.04
6.542 ± 0.049
1.808 ± 0.02
7.46 ± 0.049
6.749 ± 0.045
9.965 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.599 ± 0.025
4.548 ± 0.04
3.456 ± 0.025
4.498 ± 0.042
3.769 ± 0.039
5.853 ± 0.04
6.069 ± 0.04
6.895 ± 0.041
0.983 ± 0.019
3.663 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here