Alteribacillus iranensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alteribacillus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2DK74|A0A1I2DK74_9BACI Uncharacterized protein OS=Alteribacillus iranensis OX=930128 GN=SAMN05192532_104113 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.27RR3 pKa = 11.84LRR5 pKa = 11.84FAALFTASALFIGGCSTSGEE25 pKa = 3.74ADD27 pKa = 3.33QPKK30 pKa = 10.39EE31 pKa = 4.01STEE34 pKa = 4.12GNTQEE39 pKa = 4.39TTTEE43 pKa = 3.87ASSGGGTEE51 pKa = 3.89SGEE54 pKa = 3.97LVIYY58 pKa = 10.7SSGPGGMAEE67 pKa = 3.96EE68 pKa = 4.41LAEE71 pKa = 4.02QFEE74 pKa = 4.59EE75 pKa = 4.22QSGVEE80 pKa = 4.0VEE82 pKa = 5.0LFQSTTGDD90 pKa = 3.24VLGRR94 pKa = 11.84LEE96 pKa = 5.08AEE98 pKa = 4.21QSNPVADD105 pKa = 4.0VVVLASLQPAVDD117 pKa = 3.6YY118 pKa = 10.65MEE120 pKa = 5.29NGLIRR125 pKa = 11.84PYY127 pKa = 10.16EE128 pKa = 4.09SEE130 pKa = 4.41YY131 pKa = 11.33ADD133 pKa = 3.49QLHH136 pKa = 6.87DD137 pKa = 3.18GWYY140 pKa = 10.22DD141 pKa = 3.52EE142 pKa = 6.08DD143 pKa = 4.02YY144 pKa = 11.18HH145 pKa = 8.59FYY147 pKa = 11.06GFSAAALGLSYY158 pKa = 10.2NTDD161 pKa = 4.15LIEE164 pKa = 4.34EE165 pKa = 4.65PPRR168 pKa = 11.84EE169 pKa = 4.35WEE171 pKa = 4.28DD172 pKa = 3.58LTHH175 pKa = 6.91EE176 pKa = 5.08AYY178 pKa = 10.07QDD180 pKa = 3.54QIAIPSPSEE189 pKa = 3.75SGTARR194 pKa = 11.84DD195 pKa = 3.98FVAAYY200 pKa = 9.05IAQEE204 pKa = 4.24GEE206 pKa = 4.01EE207 pKa = 4.16EE208 pKa = 4.19GWAFFEE214 pKa = 4.13EE215 pKa = 4.62LRR217 pKa = 11.84DD218 pKa = 3.7NGLSMEE224 pKa = 4.56GSNNPALTSVINGANGVVMAGVDD247 pKa = 3.42YY248 pKa = 10.41MVYY251 pKa = 10.84NNIEE255 pKa = 3.97GGEE258 pKa = 4.0PLDD261 pKa = 3.58IVFPEE266 pKa = 4.34SGTTITPRR274 pKa = 11.84PAFILEE280 pKa = 3.98SAEE283 pKa = 3.97NVEE286 pKa = 4.24EE287 pKa = 4.07AEE289 pKa = 4.36KK290 pKa = 11.2YY291 pKa = 10.16IDD293 pKa = 5.45FILSDD298 pKa = 3.71EE299 pKa = 4.46GQGIVADD306 pKa = 4.69HH307 pKa = 6.53YY308 pKa = 11.17LLPARR313 pKa = 11.84EE314 pKa = 5.07DD315 pKa = 3.57IPPHH319 pKa = 6.05EE320 pKa = 5.49DD321 pKa = 2.6RR322 pKa = 11.84AAYY325 pKa = 10.12DD326 pKa = 4.1EE327 pKa = 4.16IAEE330 pKa = 5.1LDD332 pKa = 4.08FEE334 pKa = 4.53WEE336 pKa = 4.14YY337 pKa = 11.45LFEE340 pKa = 5.98NGEE343 pKa = 4.64DD344 pKa = 3.19ILSEE348 pKa = 4.12FTEE351 pKa = 4.24MMRR354 pKa = 4.76

Molecular weight: 38.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2C5W2|A0A1I2C5W2_9BACI DNA gyrase subunit B OS=Alteribacillus iranensis OX=930128 GN=gyrB PE=3 SV=1
MM1 pKa = 7.67GKK3 pKa = 7.97PTFKK7 pKa = 10.7PNTRR11 pKa = 11.84KK12 pKa = 9.83RR13 pKa = 11.84KK14 pKa = 8.17KK15 pKa = 8.5VHH17 pKa = 5.6GFRR20 pKa = 11.84EE21 pKa = 3.96RR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.55NGRR29 pKa = 11.84HH30 pKa = 3.86VLKK33 pKa = 10.52NRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.66GRR40 pKa = 11.84KK41 pKa = 8.69VLSAA45 pKa = 4.05

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3218

0

3218

934243

29

1495

290.3

32.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.29 ± 0.04

0.675 ± 0.014

5.256 ± 0.041

8.273 ± 0.053

4.266 ± 0.04

7.076 ± 0.037

2.292 ± 0.024

7.244 ± 0.039

6.145 ± 0.048

9.428 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.881 ± 0.02

3.968 ± 0.027

3.854 ± 0.023

3.687 ± 0.027

4.456 ± 0.03

6.099 ± 0.028

5.596 ± 0.031

7.111 ± 0.035

1.077 ± 0.019

3.325 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski