Escherichia phage chee24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus; Escherichia virus chee24

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8HP56|A0A2K8HP56_9CAUD Tail length tape-measure protein OS=Escherichia phage chee24 OX=2024330 GN=P24_0132 PE=4 SV=1
MM1 pKa = 7.47SIVKK5 pKa = 9.69SVYY8 pKa = 7.53THH10 pKa = 7.3PDD12 pKa = 3.83DD13 pKa = 4.1IFVWADD19 pKa = 3.15GSWCYY24 pKa = 10.27RR25 pKa = 11.84CEE27 pKa = 4.03LWEE30 pKa = 4.28MDD32 pKa = 4.5YY33 pKa = 11.4KK34 pKa = 11.19SDD36 pKa = 3.93DD37 pKa = 3.5YY38 pKa = 11.91GVIYY42 pKa = 10.63VDD44 pKa = 3.28TVEE47 pKa = 4.3YY48 pKa = 8.99DD49 pKa = 3.32TFLEE53 pKa = 4.23RR54 pKa = 11.84DD55 pKa = 3.42KK56 pKa = 11.68NGNKK60 pKa = 9.89

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8H8W3|A0A2K8H8W3_9CAUD Ribonucleotide reductase of class III (Anaerobic) large subunit OS=Escherichia phage chee24 OX=2024330 GN=P24_0098 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 9.77QKK5 pKa = 10.18NAKK8 pKa = 7.58TQAAPAVKK16 pKa = 9.39TFPKK20 pKa = 10.24TEE22 pKa = 4.13ANRR25 pKa = 11.84KK26 pKa = 8.94ARR28 pKa = 11.84LEE30 pKa = 3.84RR31 pKa = 11.84HH32 pKa = 5.4LRR34 pKa = 11.84KK35 pKa = 10.0HH36 pKa = 5.6PTDD39 pKa = 3.49AQAAQALNNPAPIRR53 pKa = 11.84QKK55 pKa = 10.71PKK57 pKa = 10.52AKK59 pKa = 9.68NATRR63 pKa = 11.84KK64 pKa = 8.39SYY66 pKa = 11.17KK67 pKa = 8.89MVMYY71 pKa = 10.31VEE73 pKa = 4.89NYY75 pKa = 7.4GHH77 pKa = 6.99KK78 pKa = 9.99SVPVSLSFNAGAEE91 pKa = 4.17LFSRR95 pKa = 11.84NGMSMKK101 pKa = 10.42DD102 pKa = 3.33YY103 pKa = 11.01EE104 pKa = 4.31KK105 pKa = 10.98AVNQKK110 pKa = 9.97RR111 pKa = 11.84KK112 pKa = 8.13PTADD116 pKa = 3.13VLRR119 pKa = 11.84DD120 pKa = 3.4TRR122 pKa = 11.84GQFGSVKK129 pKa = 9.73PNIFGVEE136 pKa = 3.85YY137 pKa = 11.02SKK139 pKa = 11.81DD140 pKa = 3.37NVRR143 pKa = 11.84ALCYY147 pKa = 10.2GVGIKK152 pKa = 8.51FTGDD156 pKa = 3.22SARR159 pKa = 11.84KK160 pKa = 7.85SAKK163 pKa = 9.24PARR166 pKa = 11.84KK167 pKa = 9.36RR168 pKa = 11.84KK169 pKa = 9.78AKK171 pKa = 10.37

Molecular weight: 19.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

153

0

153

30303

37

1226

198.1

22.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.917 ± 0.37

1.079 ± 0.092

6.155 ± 0.152

7.075 ± 0.231

4.052 ± 0.129

6.643 ± 0.344

1.881 ± 0.118

6.448 ± 0.173

7.184 ± 0.262

8.336 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.69 ± 0.165

5.306 ± 0.171

3.455 ± 0.129

3.911 ± 0.218

4.31 ± 0.115

6.234 ± 0.168

5.689 ± 0.16

6.564 ± 0.173

1.221 ± 0.101

3.851 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski