Amino acid dipepetide frequency for Escherichia phage chee24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.382AlaAla: 8.382 ± 1.3
0.66AlaCys: 0.66 ± 0.161
4.224AlaAsp: 4.224 ± 0.377
5.841AlaGlu: 5.841 ± 0.531
3.432AlaPhe: 3.432 ± 0.338
5.379AlaGly: 5.379 ± 0.466
1.485AlaHis: 1.485 ± 0.263
4.686AlaIle: 4.686 ± 0.396
6.996AlaLys: 6.996 ± 0.493
6.27AlaLeu: 6.27 ± 0.407
2.112AlaMet: 2.112 ± 0.279
3.531AlaAsn: 3.531 ± 0.346
2.31AlaPro: 2.31 ± 0.294
3.861AlaGln: 3.861 ± 0.405
3.168AlaArg: 3.168 ± 0.258
4.884AlaSer: 4.884 ± 0.6
3.828AlaThr: 3.828 ± 0.425
4.851AlaVal: 4.851 ± 0.592
0.858AlaTrp: 0.858 ± 0.17
2.376AlaTyr: 2.376 ± 0.326
0.0AlaXaa: 0.0 ± 0.0
Cys
0.627CysAla: 0.627 ± 0.179
0.231CysCys: 0.231 ± 0.076
0.594CysAsp: 0.594 ± 0.12
0.759CysGlu: 0.759 ± 0.15
0.462CysPhe: 0.462 ± 0.119
0.825CysGly: 0.825 ± 0.188
0.33CysHis: 0.33 ± 0.091
0.792CysIle: 0.792 ± 0.167
0.693CysLys: 0.693 ± 0.132
0.726CysLeu: 0.726 ± 0.153
0.33CysMet: 0.33 ± 0.11
0.627CysAsn: 0.627 ± 0.131
0.429CysPro: 0.429 ± 0.103
0.363CysGln: 0.363 ± 0.109
0.66CysArg: 0.66 ± 0.152
0.792CysSer: 0.792 ± 0.14
0.495CysThr: 0.495 ± 0.122
0.594CysVal: 0.594 ± 0.147
0.099CysTrp: 0.099 ± 0.052
0.363CysTyr: 0.363 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
4.719AspAla: 4.719 ± 0.364
0.66AspCys: 0.66 ± 0.131
3.135AspAsp: 3.135 ± 0.394
4.554AspGlu: 4.554 ± 0.393
2.706AspPhe: 2.706 ± 0.273
4.125AspGly: 4.125 ± 0.369
1.122AspHis: 1.122 ± 0.185
4.653AspIle: 4.653 ± 0.42
4.488AspLys: 4.488 ± 0.419
5.544AspLeu: 5.544 ± 0.423
1.947AspMet: 1.947 ± 0.249
2.706AspAsn: 2.706 ± 0.35
2.475AspPro: 2.475 ± 0.214
1.683AspGln: 1.683 ± 0.194
2.541AspArg: 2.541 ± 0.321
3.63AspSer: 3.63 ± 0.316
3.696AspThr: 3.696 ± 0.393
3.597AspVal: 3.597 ± 0.357
0.891AspTrp: 0.891 ± 0.179
2.673AspTyr: 2.673 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
6.369GluAla: 6.369 ± 0.501
0.693GluCys: 0.693 ± 0.176
4.092GluAsp: 4.092 ± 0.318
5.643GluGlu: 5.643 ± 0.456
2.442GluPhe: 2.442 ± 0.254
3.828GluGly: 3.828 ± 0.391
1.419GluHis: 1.419 ± 0.211
4.554GluIle: 4.554 ± 0.381
4.95GluLys: 4.95 ± 0.423
7.458GluLeu: 7.458 ± 0.51
2.805GluMet: 2.805 ± 0.374
2.706GluAsn: 2.706 ± 0.289
1.584GluPro: 1.584 ± 0.255
3.3GluGln: 3.3 ± 0.373
2.937GluArg: 2.937 ± 0.331
3.3GluSer: 3.3 ± 0.323
3.96GluThr: 3.96 ± 0.309
4.422GluVal: 4.422 ± 0.356
1.287GluTrp: 1.287 ± 0.19
3.003GluTyr: 3.003 ± 0.304
0.0GluXaa: 0.0 ± 0.0
Phe
2.871PheAla: 2.871 ± 0.36
0.429PheCys: 0.429 ± 0.114
2.772PheAsp: 2.772 ± 0.299
2.541PheGlu: 2.541 ± 0.328
1.221PhePhe: 1.221 ± 0.187
3.036PheGly: 3.036 ± 0.345
1.221PheHis: 1.221 ± 0.219
2.64PheIle: 2.64 ± 0.361
2.706PheLys: 2.706 ± 0.309
3.333PheLeu: 3.333 ± 0.364
0.726PheMet: 0.726 ± 0.147
2.607PheAsn: 2.607 ± 0.266
1.584PhePro: 1.584 ± 0.234
0.99PheGln: 0.99 ± 0.137
1.815PheArg: 1.815 ± 0.301
2.838PheSer: 2.838 ± 0.288
2.178PheThr: 2.178 ± 0.255
2.97PheVal: 2.97 ± 0.306
0.528PheTrp: 0.528 ± 0.148
1.518PheTyr: 1.518 ± 0.212
0.0PheXaa: 0.0 ± 0.0
Gly
5.379GlyAla: 5.379 ± 0.606
0.891GlyCys: 0.891 ± 0.202
3.795GlyAsp: 3.795 ± 0.393
4.422GlyGlu: 4.422 ± 0.307
3.498GlyPhe: 3.498 ± 0.331
3.96GlyGly: 3.96 ± 0.647
1.089GlyHis: 1.089 ± 0.181
4.554GlyIle: 4.554 ± 0.382
5.181GlyLys: 5.181 ± 0.419
5.049GlyLeu: 5.049 ± 0.37
2.046GlyMet: 2.046 ± 0.236
3.762GlyAsn: 3.762 ± 0.536
1.122GlyPro: 1.122 ± 0.171
2.178GlyGln: 2.178 ± 0.27
2.607GlyArg: 2.607 ± 0.281
4.092GlySer: 4.092 ± 0.451
3.993GlyThr: 3.993 ± 0.498
4.719GlyVal: 4.719 ± 0.381
1.122GlyTrp: 1.122 ± 0.218
2.97GlyTyr: 2.97 ± 0.287
0.0GlyXaa: 0.0 ± 0.0
His
1.221HisAla: 1.221 ± 0.223
0.264HisCys: 0.264 ± 0.088
1.551HisAsp: 1.551 ± 0.251
1.221HisGlu: 1.221 ± 0.201
0.792HisPhe: 0.792 ± 0.188
1.32HisGly: 1.32 ± 0.231
0.495HisHis: 0.495 ± 0.163
1.353HisIle: 1.353 ± 0.219
1.221HisLys: 1.221 ± 0.192
1.815HisLeu: 1.815 ± 0.247
0.396HisMet: 0.396 ± 0.111
0.858HisAsn: 0.858 ± 0.188
0.99HisPro: 0.99 ± 0.195
0.495HisGln: 0.495 ± 0.124
1.023HisArg: 1.023 ± 0.205
1.056HisSer: 1.056 ± 0.195
0.957HisThr: 0.957 ± 0.19
0.825HisVal: 0.825 ± 0.194
0.165HisTrp: 0.165 ± 0.07
0.792HisTyr: 0.792 ± 0.165
0.0HisXaa: 0.0 ± 0.0
Ile
5.082IleAla: 5.082 ± 0.396
1.023IleCys: 1.023 ± 0.212
4.752IleAsp: 4.752 ± 0.415
4.686IleGlu: 4.686 ± 0.328
1.947IlePhe: 1.947 ± 0.24
3.993IleGly: 3.993 ± 0.399
1.089IleHis: 1.089 ± 0.15
4.026IleIle: 4.026 ± 0.386
4.62IleLys: 4.62 ± 0.384
5.049IleLeu: 5.049 ± 0.444
1.683IleMet: 1.683 ± 0.236
3.927IleAsn: 3.927 ± 0.376
2.409IlePro: 2.409 ± 0.351
2.343IleGln: 2.343 ± 0.305
2.937IleArg: 2.937 ± 0.296
4.389IleSer: 4.389 ± 0.339
4.422IleThr: 4.422 ± 0.338
3.564IleVal: 3.564 ± 0.358
0.528IleTrp: 0.528 ± 0.13
2.013IleTyr: 2.013 ± 0.271
0.0IleXaa: 0.0 ± 0.0
Lys
6.468LysAla: 6.468 ± 0.54
0.396LysCys: 0.396 ± 0.143
4.488LysAsp: 4.488 ± 0.373
4.917LysGlu: 4.917 ± 0.462
3.3LysPhe: 3.3 ± 0.338
3.234LysGly: 3.234 ± 0.303
1.221LysHis: 1.221 ± 0.224
3.729LysIle: 3.729 ± 0.366
4.719LysLys: 4.719 ± 0.458
6.732LysLeu: 6.732 ± 0.527
2.442LysMet: 2.442 ± 0.289
3.597LysAsn: 3.597 ± 0.414
2.376LysPro: 2.376 ± 0.28
3.465LysGln: 3.465 ± 0.348
3.432LysArg: 3.432 ± 0.31
4.422LysSer: 4.422 ± 0.397
3.894LysThr: 3.894 ± 0.491
5.148LysVal: 5.148 ± 0.447
0.627LysTrp: 0.627 ± 0.141
3.234LysTyr: 3.234 ± 0.307
0.0LysXaa: 0.0 ± 0.0
Leu
7.128LeuAla: 7.128 ± 0.507
0.759LeuCys: 0.759 ± 0.146
5.973LeuAsp: 5.973 ± 0.391
7.227LeuGlu: 7.227 ± 0.587
2.343LeuPhe: 2.343 ± 0.28
5.544LeuGly: 5.544 ± 0.432
1.947LeuHis: 1.947 ± 0.267
5.115LeuIle: 5.115 ± 0.465
6.27LeuLys: 6.27 ± 0.423
6.039LeuLeu: 6.039 ± 0.555
2.079LeuMet: 2.079 ± 0.317
5.181LeuAsn: 5.181 ± 0.424
3.498LeuPro: 3.498 ± 0.383
3.333LeuGln: 3.333 ± 0.417
3.498LeuArg: 3.498 ± 0.236
4.719LeuSer: 4.719 ± 0.391
4.422LeuThr: 4.422 ± 0.401
5.115LeuVal: 5.115 ± 0.496
0.594LeuTrp: 0.594 ± 0.123
2.574LeuTyr: 2.574 ± 0.289
0.0LeuXaa: 0.0 ± 0.0
Met
1.683MetAla: 1.683 ± 0.216
0.33MetCys: 0.33 ± 0.101
1.518MetAsp: 1.518 ± 0.232
2.211MetGlu: 2.211 ± 0.294
0.759MetPhe: 0.759 ± 0.169
1.584MetGly: 1.584 ± 0.214
0.462MetHis: 0.462 ± 0.129
1.815MetIle: 1.815 ± 0.305
2.574MetLys: 2.574 ± 0.322
2.145MetLeu: 2.145 ± 0.315
0.429MetMet: 0.429 ± 0.111
1.122MetAsn: 1.122 ± 0.176
0.99MetPro: 0.99 ± 0.164
1.386MetGln: 1.386 ± 0.228
1.221MetArg: 1.221 ± 0.169
2.409MetSer: 2.409 ± 0.296
1.749MetThr: 1.749 ± 0.277
1.419MetVal: 1.419 ± 0.208
0.165MetTrp: 0.165 ± 0.071
0.924MetTyr: 0.924 ± 0.182
0.0MetXaa: 0.0 ± 0.0
Asn
3.564AsnAla: 3.564 ± 0.371
0.528AsnCys: 0.528 ± 0.136
3.003AsnAsp: 3.003 ± 0.319
2.904AsnGlu: 2.904 ± 0.33
2.178AsnPhe: 2.178 ± 0.246
4.62AsnGly: 4.62 ± 0.523
0.825AsnHis: 0.825 ± 0.19
3.861AsnIle: 3.861 ± 0.389
3.531AsnLys: 3.531 ± 0.343
4.356AsnLeu: 4.356 ± 0.359
0.924AsnMet: 0.924 ± 0.164
3.168AsnAsn: 3.168 ± 0.359
2.673AsnPro: 2.673 ± 0.315
1.848AsnGln: 1.848 ± 0.269
2.343AsnArg: 2.343 ± 0.294
3.762AsnSer: 3.762 ± 0.409
3.003AsnThr: 3.003 ± 0.316
3.663AsnVal: 3.663 ± 0.341
0.561AsnTrp: 0.561 ± 0.149
1.749AsnTyr: 1.749 ± 0.246
0.0AsnXaa: 0.0 ± 0.0
Pro
2.904ProAla: 2.904 ± 0.281
0.396ProCys: 0.396 ± 0.113
2.64ProAsp: 2.64 ± 0.263
3.003ProGlu: 3.003 ± 0.375
1.452ProPhe: 1.452 ± 0.214
2.31ProGly: 2.31 ± 0.283
0.726ProHis: 0.726 ± 0.173
2.178ProIle: 2.178 ± 0.275
1.914ProLys: 1.914 ± 0.236
2.046ProLeu: 2.046 ± 0.241
0.396ProMet: 0.396 ± 0.123
1.914ProAsn: 1.914 ± 0.265
1.386ProPro: 1.386 ± 0.256
1.023ProGln: 1.023 ± 0.198
1.386ProArg: 1.386 ± 0.196
1.716ProSer: 1.716 ± 0.257
2.112ProThr: 2.112 ± 0.239
2.772ProVal: 2.772 ± 0.274
0.429ProTrp: 0.429 ± 0.101
1.848ProTyr: 1.848 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
3.201GlnAla: 3.201 ± 0.472
0.594GlnCys: 0.594 ± 0.132
2.112GlnAsp: 2.112 ± 0.27
2.772GlnGlu: 2.772 ± 0.322
1.683GlnPhe: 1.683 ± 0.233
1.914GlnGly: 1.914 ± 0.215
0.528GlnHis: 0.528 ± 0.142
2.508GlnIle: 2.508 ± 0.296
2.607GlnLys: 2.607 ± 0.332
3.927GlnLeu: 3.927 ± 0.486
1.056GlnMet: 1.056 ± 0.258
1.551GlnAsn: 1.551 ± 0.225
0.594GlnPro: 0.594 ± 0.153
2.838GlnGln: 2.838 ± 0.642
1.815GlnArg: 1.815 ± 0.296
2.376GlnSer: 2.376 ± 0.285
2.277GlnThr: 2.277 ± 0.261
2.64GlnVal: 2.64 ± 0.32
0.561GlnTrp: 0.561 ± 0.142
1.551GlnTyr: 1.551 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
3.102ArgAla: 3.102 ± 0.328
0.33ArgCys: 0.33 ± 0.102
2.838ArgAsp: 2.838 ± 0.288
2.871ArgGlu: 2.871 ± 0.307
1.782ArgPhe: 1.782 ± 0.255
3.168ArgGly: 3.168 ± 0.282
0.594ArgHis: 0.594 ± 0.149
2.937ArgIle: 2.937 ± 0.291
2.805ArgLys: 2.805 ± 0.406
3.795ArgLeu: 3.795 ± 0.318
1.485ArgMet: 1.485 ± 0.248
2.607ArgAsn: 2.607 ± 0.273
1.32ArgPro: 1.32 ± 0.204
1.584ArgGln: 1.584 ± 0.231
2.079ArgArg: 2.079 ± 0.262
2.211ArgSer: 2.211 ± 0.219
2.541ArgThr: 2.541 ± 0.236
2.97ArgVal: 2.97 ± 0.261
0.396ArgTrp: 0.396 ± 0.123
1.683ArgTyr: 1.683 ± 0.281
0.0ArgXaa: 0.0 ± 0.0
Ser
4.191SerAla: 4.191 ± 0.459
0.627SerCys: 0.627 ± 0.124
2.937SerAsp: 2.937 ± 0.277
3.96SerGlu: 3.96 ± 0.401
3.168SerPhe: 3.168 ± 0.253
5.412SerGly: 5.412 ± 0.482
0.759SerHis: 0.759 ± 0.152
4.125SerIle: 4.125 ± 0.422
4.158SerLys: 4.158 ± 0.319
5.874SerLeu: 5.874 ± 0.384
1.518SerMet: 1.518 ± 0.23
3.366SerAsn: 3.366 ± 0.359
2.112SerPro: 2.112 ± 0.317
1.848SerGln: 1.848 ± 0.26
2.871SerArg: 2.871 ± 0.262
4.026SerSer: 4.026 ± 0.395
3.531SerThr: 3.531 ± 0.344
4.191SerVal: 4.191 ± 0.321
1.188SerTrp: 1.188 ± 0.174
2.475SerTyr: 2.475 ± 0.309
0.0SerXaa: 0.0 ± 0.0
Thr
4.158ThrAla: 4.158 ± 0.508
0.462ThrCys: 0.462 ± 0.108
3.234ThrAsp: 3.234 ± 0.271
3.465ThrGlu: 3.465 ± 0.278
2.31ThrPhe: 2.31 ± 0.278
4.818ThrGly: 4.818 ± 0.507
0.924ThrHis: 0.924 ± 0.194
4.026ThrIle: 4.026 ± 0.433
3.498ThrLys: 3.498 ± 0.377
4.686ThrLeu: 4.686 ± 0.401
1.485ThrMet: 1.485 ± 0.225
2.937ThrAsn: 2.937 ± 0.32
2.409ThrPro: 2.409 ± 0.244
2.211ThrGln: 2.211 ± 0.252
2.244ThrArg: 2.244 ± 0.235
3.696ThrSer: 3.696 ± 0.327
3.069ThrThr: 3.069 ± 0.399
4.488ThrVal: 4.488 ± 0.412
0.693ThrTrp: 0.693 ± 0.178
2.079ThrTyr: 2.079 ± 0.306
0.0ThrXaa: 0.0 ± 0.0
Val
5.412ValAla: 5.412 ± 0.457
0.594ValCys: 0.594 ± 0.151
4.092ValAsp: 4.092 ± 0.458
4.521ValGlu: 4.521 ± 0.319
2.838ValPhe: 2.838 ± 0.284
4.125ValGly: 4.125 ± 0.371
1.089ValHis: 1.089 ± 0.182
4.257ValIle: 4.257 ± 0.306
4.818ValLys: 4.818 ± 0.346
4.719ValLeu: 4.719 ± 0.339
1.65ValMet: 1.65 ± 0.213
3.465ValAsn: 3.465 ± 0.386
2.706ValPro: 2.706 ± 0.325
2.31ValGln: 2.31 ± 0.253
2.475ValArg: 2.475 ± 0.269
4.686ValSer: 4.686 ± 0.421
3.795ValThr: 3.795 ± 0.341
4.686ValVal: 4.686 ± 0.414
0.759ValTrp: 0.759 ± 0.187
2.64ValTyr: 2.64 ± 0.295
0.0ValXaa: 0.0 ± 0.0
Trp
0.429TrpAla: 0.429 ± 0.123
0.231TrpCys: 0.231 ± 0.098
0.924TrpAsp: 0.924 ± 0.159
1.188TrpGlu: 1.188 ± 0.223
0.627TrpPhe: 0.627 ± 0.152
0.693TrpGly: 0.693 ± 0.19
0.198TrpHis: 0.198 ± 0.072
0.561TrpIle: 0.561 ± 0.13
0.957TrpLys: 0.957 ± 0.149
1.155TrpLeu: 1.155 ± 0.201
0.33TrpMet: 0.33 ± 0.108
0.66TrpAsn: 0.66 ± 0.128
0.264TrpPro: 0.264 ± 0.114
0.627TrpGln: 0.627 ± 0.123
0.528TrpArg: 0.528 ± 0.123
0.759TrpSer: 0.759 ± 0.182
0.594TrpThr: 0.594 ± 0.159
0.792TrpVal: 0.792 ± 0.159
0.231TrpTrp: 0.231 ± 0.101
0.462TrpTyr: 0.462 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.277TyrAla: 2.277 ± 0.269
0.693TyrCys: 0.693 ± 0.148
2.871TyrAsp: 2.871 ± 0.285
2.046TyrGlu: 2.046 ± 0.243
1.584TyrPhe: 1.584 ± 0.252
2.541TyrGly: 2.541 ± 0.289
1.287TyrHis: 1.287 ± 0.186
2.112TyrIle: 2.112 ± 0.279
3.135TyrLys: 3.135 ± 0.259
2.64TyrLeu: 2.64 ± 0.277
1.023TyrMet: 1.023 ± 0.184
2.772TyrAsn: 2.772 ± 0.278
1.32TyrPro: 1.32 ± 0.155
1.419TyrGln: 1.419 ± 0.24
1.518TyrArg: 1.518 ± 0.207
2.574TyrSer: 2.574 ± 0.262
2.376TyrThr: 2.376 ± 0.228
2.211TyrVal: 2.211 ± 0.226
0.528TyrTrp: 0.528 ± 0.133
1.584TyrTyr: 1.584 ± 0.254
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 153 proteins (30303 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski