Streptococcus satellite phage Javan176
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKY7|A0A4D5ZKY7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan176 OX=2558556 GN=JavanS176_0007 PE=4 SV=1
MM1 pKa = 7.65 TGTDD5 pKa = 3.83 FLGQEE10 pKa = 4.64 LEE12 pKa = 4.05 NMDD15 pKa = 4.13 RR16 pKa = 11.84 QRR18 pKa = 11.84 AIYY21 pKa = 8.71 MADD24 pKa = 3.46 DD25 pKa = 3.93 TPVTDD30 pKa = 3.74 EE31 pKa = 5.84 DD32 pKa = 3.82 IPKK35 pKa = 10.28 LLEE38 pKa = 4.2 IGAKK42 pKa = 9.87 LRR44 pKa = 11.84 EE45 pKa = 4.54 ADD47 pKa = 3.46 PSLNVYY53 pKa = 9.15 EE54 pKa = 4.79 LYY56 pKa = 10.56 KK57 pKa = 10.79 HH58 pKa = 6.33 PEE60 pKa = 3.45 ARR62 pKa = 11.84 AKK64 pKa = 10.61 LFAQITEE71 pKa = 4.05 ACYY74 pKa = 9.14 MALGKK79 pKa = 8.66 TPTQAQRR86 pKa = 11.84 LKK88 pKa = 10.56 FGEE91 pKa = 4.11 YY92 pKa = 10.68 LEE94 pKa = 5.19 GQFKK98 pKa = 8.36 TTMQKK103 pKa = 10.69 LINQTDD109 pKa = 3.77 TQALGEE115 pKa = 4.37 LIEE118 pKa = 4.12 VLEE121 pKa = 4.22 LDD123 pKa = 3.9 GAQRR127 pKa = 11.84 EE128 pKa = 4.28 QFAKK132 pKa = 10.85 DD133 pKa = 3.34 MAVSGLLARR142 pKa = 11.84 NEE144 pKa = 3.79 VDD146 pKa = 3.3 EE147 pKa = 4.45
Molecular weight: 16.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.615
IPC2_protein 4.546
IPC_protein 4.444
Toseland 4.279
ProMoST 4.495
Dawson 4.38
Bjellqvist 4.571
Wikipedia 4.24
Rodwell 4.279
Grimsley 4.19
Solomon 4.38
Lehninger 4.329
Nozaki 4.495
DTASelect 4.635
Thurlkill 4.291
EMBOSS 4.266
Sillero 4.546
Patrickios 4.012
IPC_peptide 4.38
IPC2_peptide 4.546
IPC2.peptide.svr19 4.479
Protein with the highest isoelectric point:
>tr|A0A4D5ZK46|A0A4D5ZK46_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan176 OX=2558556 GN=JavanS176_0011 PE=4 SV=1
MM1 pKa = 7.3 PQATQTNEE9 pKa = 3.67 KK10 pKa = 9.99 KK11 pKa = 10.8 VNQTTSYY18 pKa = 8.71 PARR21 pKa = 11.84 EE22 pKa = 4.29 IGTHH26 pKa = 5.39 RR27 pKa = 11.84 LKK29 pKa = 10.84 SLYY32 pKa = 10.05 GFYY35 pKa = 10.24 MSLL38 pKa = 3.4
Molecular weight: 4.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.144
IPC2_protein 9.385
IPC_protein 9.472
Toseland 9.692
ProMoST 9.516
Dawson 9.984
Bjellqvist 9.692
Wikipedia 10.204
Rodwell 10.218
Grimsley 10.087
Solomon 10.072
Lehninger 10.028
Nozaki 9.633
DTASelect 9.706
Thurlkill 9.794
EMBOSS 10.101
Sillero 9.882
Patrickios 7.556
IPC_peptide 10.058
IPC2_peptide 8.375
IPC2.peptide.svr19 8.246
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
2652
38
387
165.8
19.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.863 ± 0.677
0.49 ± 0.146
5.92 ± 0.572
8.861 ± 0.906
3.846 ± 0.36
4.374 ± 0.508
1.32 ± 0.298
5.845 ± 0.329
8.183 ± 0.521
9.917 ± 0.539
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.526 ± 0.34
4.487 ± 0.356
2.866 ± 0.349
5.882 ± 0.571
5.468 ± 0.476
6.712 ± 0.538
5.656 ± 0.562
5.354 ± 0.503
0.905 ± 0.199
4.525 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here