Salmonella phage SE20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Epseptimavirus; unclassified Epseptimavirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0CG35|A0A5C0CG35_9CAUD Uncharacterized protein OS=Salmonella phage SE20 OX=2592199 PE=4 SV=1
MM1 pKa = 7.51NIVLPKK7 pKa = 10.25TLDD10 pKa = 2.99KK11 pKa = 10.78KK12 pKa = 10.99QYY14 pKa = 8.27ITPDD18 pKa = 3.18DD19 pKa = 3.96MEE21 pKa = 5.2CGKK24 pKa = 10.62VYY26 pKa = 10.73QFDD29 pKa = 4.02YY30 pKa = 10.83MLASPTNTGCIIAVDD45 pKa = 3.67TRR47 pKa = 11.84DD48 pKa = 3.58NDD50 pKa = 3.56VLTFNDD56 pKa = 3.68YY57 pKa = 11.38SDD59 pKa = 3.65FMEE62 pKa = 4.4WLDD65 pKa = 4.7DD66 pKa = 4.49QSITNNGEE74 pKa = 3.68LTPYY78 pKa = 10.58QLTIAIKK85 pKa = 10.26

Molecular weight: 9.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0CGB1|A0A5C0CGB1_9CAUD Putative serine/threonine protein phosphatase OS=Salmonella phage SE20 OX=2592199 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 9.77QKK5 pKa = 10.18NAKK8 pKa = 7.58TQAAPAVKK16 pKa = 9.57TFAQTEE22 pKa = 4.28ANRR25 pKa = 11.84KK26 pKa = 8.94ARR28 pKa = 11.84LEE30 pKa = 3.84RR31 pKa = 11.84HH32 pKa = 5.4LRR34 pKa = 11.84KK35 pKa = 10.0HH36 pKa = 5.61PTDD39 pKa = 3.63AQAQTALKK47 pKa = 9.72SPAPIRR53 pKa = 11.84QKK55 pKa = 10.77PKK57 pKa = 10.34AKK59 pKa = 10.31NSTRR63 pKa = 11.84ATAKK67 pKa = 9.62QVVFIKK73 pKa = 11.09GEE75 pKa = 3.99GHH77 pKa = 6.3KK78 pKa = 10.36SVPVTLSFNGGAEE91 pKa = 3.83MFARR95 pKa = 11.84NGMALKK101 pKa = 10.34DD102 pKa = 3.61YY103 pKa = 10.55EE104 pKa = 4.21KK105 pKa = 10.99AVNQKK110 pKa = 9.22RR111 pKa = 11.84KK112 pKa = 9.85PMADD116 pKa = 2.87VMRR119 pKa = 11.84EE120 pKa = 3.73SRR122 pKa = 11.84GQFGSVKK129 pKa = 9.73PNIFGVEE136 pKa = 3.77YY137 pKa = 10.96SRR139 pKa = 11.84DD140 pKa = 3.43NVRR143 pKa = 11.84ALCYY147 pKa = 10.52GIGIKK152 pKa = 9.02FTGASDD158 pKa = 3.56RR159 pKa = 11.84KK160 pKa = 9.12SAKK163 pKa = 9.32PARR166 pKa = 11.84KK167 pKa = 9.36RR168 pKa = 11.84KK169 pKa = 9.78AKK171 pKa = 10.37

Molecular weight: 18.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

149

0

149

29009

38

1226

194.7

21.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.056 ± 0.449

0.972 ± 0.098

6.367 ± 0.165

7.074 ± 0.203

4.181 ± 0.146

6.446 ± 0.209

1.813 ± 0.145

6.519 ± 0.152

7.16 ± 0.194

8.397 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.146

5.116 ± 0.166

3.485 ± 0.131

3.657 ± 0.179

4.392 ± 0.139

6.229 ± 0.191

5.867 ± 0.216

6.47 ± 0.169

1.189 ± 0.095

3.868 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski