Amino acid dipepetide frequency for Salmonella phage SE20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.308AlaAla: 9.308 ± 1.309
0.655AlaCys: 0.655 ± 0.141
4.309AlaAsp: 4.309 ± 0.405
5.654AlaGlu: 5.654 ± 0.489
3.034AlaPhe: 3.034 ± 0.346
5.654AlaGly: 5.654 ± 0.554
1.138AlaHis: 1.138 ± 0.201
4.964AlaIle: 4.964 ± 0.372
7.033AlaLys: 7.033 ± 0.512
6.757AlaLeu: 6.757 ± 0.465
2.103AlaMet: 2.103 ± 0.343
3.62AlaAsn: 3.62 ± 0.363
2.585AlaPro: 2.585 ± 0.362
3.413AlaGln: 3.413 ± 0.457
3.309AlaArg: 3.309 ± 0.312
4.792AlaSer: 4.792 ± 0.562
4.275AlaThr: 4.275 ± 0.576
4.619AlaVal: 4.619 ± 0.413
0.827AlaTrp: 0.827 ± 0.162
2.517AlaTyr: 2.517 ± 0.301
0.0AlaXaa: 0.0 ± 0.0
Cys
0.414CysAla: 0.414 ± 0.135
0.207CysCys: 0.207 ± 0.1
0.655CysAsp: 0.655 ± 0.169
0.621CysGlu: 0.621 ± 0.135
0.517CysPhe: 0.517 ± 0.145
0.931CysGly: 0.931 ± 0.202
0.172CysHis: 0.172 ± 0.072
0.621CysIle: 0.621 ± 0.116
0.793CysLys: 0.793 ± 0.168
0.862CysLeu: 0.862 ± 0.161
0.172CysMet: 0.172 ± 0.076
0.483CysAsn: 0.483 ± 0.154
0.379CysPro: 0.379 ± 0.123
0.31CysGln: 0.31 ± 0.124
0.448CysArg: 0.448 ± 0.125
0.724CysSer: 0.724 ± 0.171
0.448CysThr: 0.448 ± 0.136
0.621CysVal: 0.621 ± 0.144
0.069CysTrp: 0.069 ± 0.054
0.276CysTyr: 0.276 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
4.723AspAla: 4.723 ± 0.358
0.483AspCys: 0.483 ± 0.157
3.24AspAsp: 3.24 ± 0.441
4.964AspGlu: 4.964 ± 0.43
2.999AspPhe: 2.999 ± 0.348
3.482AspGly: 3.482 ± 0.424
0.931AspHis: 0.931 ± 0.184
4.516AspIle: 4.516 ± 0.395
4.482AspLys: 4.482 ± 0.41
5.481AspLeu: 5.481 ± 0.505
2.344AspMet: 2.344 ± 0.275
2.482AspAsn: 2.482 ± 0.299
3.103AspPro: 3.103 ± 0.309
1.655AspGln: 1.655 ± 0.233
2.585AspArg: 2.585 ± 0.346
3.827AspSer: 3.827 ± 0.414
4.102AspThr: 4.102 ± 0.456
4.275AspVal: 4.275 ± 0.409
0.931AspTrp: 0.931 ± 0.184
3.068AspTyr: 3.068 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
5.86GluAla: 5.86 ± 0.42
0.655GluCys: 0.655 ± 0.172
4.171GluAsp: 4.171 ± 0.389
5.55GluGlu: 5.55 ± 0.501
2.827GluPhe: 2.827 ± 0.356
3.654GluGly: 3.654 ± 0.358
1.62GluHis: 1.62 ± 0.228
5.274GluIle: 5.274 ± 0.469
4.999GluLys: 4.999 ± 0.458
7.205GluLeu: 7.205 ± 0.48
2.379GluMet: 2.379 ± 0.323
2.448GluAsn: 2.448 ± 0.293
1.62GluPro: 1.62 ± 0.266
3.172GluGln: 3.172 ± 0.474
3.309GluArg: 3.309 ± 0.396
3.551GluSer: 3.551 ± 0.305
4.171GluThr: 4.171 ± 0.365
4.378GluVal: 4.378 ± 0.353
0.965GluTrp: 0.965 ± 0.204
2.896GluTyr: 2.896 ± 0.288
0.0GluXaa: 0.0 ± 0.0
Phe
2.723PheAla: 2.723 ± 0.275
0.414PheCys: 0.414 ± 0.119
2.999PheAsp: 2.999 ± 0.361
2.723PheGlu: 2.723 ± 0.371
1.551PhePhe: 1.551 ± 0.245
2.965PheGly: 2.965 ± 0.363
1.276PheHis: 1.276 ± 0.215
2.723PheIle: 2.723 ± 0.36
3.137PheLys: 3.137 ± 0.324
2.999PheLeu: 2.999 ± 0.272
0.965PheMet: 0.965 ± 0.179
2.758PheAsn: 2.758 ± 0.33
1.758PhePro: 1.758 ± 0.256
1.103PheGln: 1.103 ± 0.211
1.965PheArg: 1.965 ± 0.309
2.723PheSer: 2.723 ± 0.257
2.413PheThr: 2.413 ± 0.267
2.585PheVal: 2.585 ± 0.32
0.483PheTrp: 0.483 ± 0.144
1.551PheTyr: 1.551 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
4.619GlyAla: 4.619 ± 0.481
0.896GlyCys: 0.896 ± 0.185
3.792GlyAsp: 3.792 ± 0.431
5.033GlyGlu: 5.033 ± 0.424
3.034GlyPhe: 3.034 ± 0.263
3.516GlyGly: 3.516 ± 0.377
1.344GlyHis: 1.344 ± 0.275
4.516GlyIle: 4.516 ± 0.409
5.033GlyLys: 5.033 ± 0.424
4.413GlyLeu: 4.413 ± 0.42
1.931GlyMet: 1.931 ± 0.269
3.758GlyAsn: 3.758 ± 0.451
0.862GlyPro: 0.862 ± 0.172
2.585GlyGln: 2.585 ± 0.337
2.896GlyArg: 2.896 ± 0.328
3.895GlySer: 3.895 ± 0.393
3.482GlyThr: 3.482 ± 0.393
4.792GlyVal: 4.792 ± 0.348
0.931GlyTrp: 0.931 ± 0.185
3.137GlyTyr: 3.137 ± 0.34
0.0GlyXaa: 0.0 ± 0.0
His
1.103HisAla: 1.103 ± 0.235
0.31HisCys: 0.31 ± 0.108
1.413HisAsp: 1.413 ± 0.23
1.034HisGlu: 1.034 ± 0.181
0.724HisPhe: 0.724 ± 0.169
1.207HisGly: 1.207 ± 0.209
0.655HisHis: 0.655 ± 0.164
1.655HisIle: 1.655 ± 0.252
1.069HisLys: 1.069 ± 0.185
1.655HisLeu: 1.655 ± 0.234
0.345HisMet: 0.345 ± 0.135
0.862HisAsn: 0.862 ± 0.195
0.931HisPro: 0.931 ± 0.226
0.379HisGln: 0.379 ± 0.118
0.965HisArg: 0.965 ± 0.182
1.379HisSer: 1.379 ± 0.225
0.793HisThr: 0.793 ± 0.179
0.621HisVal: 0.621 ± 0.164
0.172HisTrp: 0.172 ± 0.087
0.862HisTyr: 0.862 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
5.171IleAla: 5.171 ± 0.444
0.655IleCys: 0.655 ± 0.176
5.068IleAsp: 5.068 ± 0.47
4.24IleGlu: 4.24 ± 0.359
2.275IlePhe: 2.275 ± 0.275
4.171IleGly: 4.171 ± 0.406
1.138IleHis: 1.138 ± 0.174
3.689IleIle: 3.689 ± 0.333
4.93IleLys: 4.93 ± 0.433
4.93IleLeu: 4.93 ± 0.36
2.034IleMet: 2.034 ± 0.297
3.861IleAsn: 3.861 ± 0.411
2.62IlePro: 2.62 ± 0.358
2.206IleGln: 2.206 ± 0.258
2.517IleArg: 2.517 ± 0.241
4.102IleSer: 4.102 ± 0.349
4.585IleThr: 4.585 ± 0.415
3.895IleVal: 3.895 ± 0.381
0.827IleTrp: 0.827 ± 0.187
2.275IleTyr: 2.275 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
6.722LysAla: 6.722 ± 0.536
0.414LysCys: 0.414 ± 0.123
4.619LysAsp: 4.619 ± 0.375
5.24LysGlu: 5.24 ± 0.441
3.413LysPhe: 3.413 ± 0.337
3.516LysGly: 3.516 ± 0.358
1.241LysHis: 1.241 ± 0.254
3.447LysIle: 3.447 ± 0.391
4.068LysLys: 4.068 ± 0.539
6.929LysLeu: 6.929 ± 0.549
2.654LysMet: 2.654 ± 0.337
3.413LysAsn: 3.413 ± 0.343
2.689LysPro: 2.689 ± 0.377
3.137LysGln: 3.137 ± 0.299
3.482LysArg: 3.482 ± 0.304
4.033LysSer: 4.033 ± 0.413
3.964LysThr: 3.964 ± 0.382
4.826LysVal: 4.826 ± 0.492
0.655LysTrp: 0.655 ± 0.147
3.137LysTyr: 3.137 ± 0.308
0.0LysXaa: 0.0 ± 0.0
Leu
7.343LeuAla: 7.343 ± 0.575
0.724LeuCys: 0.724 ± 0.154
6.826LeuAsp: 6.826 ± 0.486
7.239LeuGlu: 7.239 ± 0.489
2.861LeuPhe: 2.861 ± 0.317
5.343LeuGly: 5.343 ± 0.512
1.551LeuHis: 1.551 ± 0.242
4.723LeuIle: 4.723 ± 0.469
6.033LeuLys: 6.033 ± 0.452
6.171LeuLeu: 6.171 ± 0.434
1.793LeuMet: 1.793 ± 0.317
4.964LeuAsn: 4.964 ± 0.395
3.516LeuPro: 3.516 ± 0.408
3.137LeuGln: 3.137 ± 0.33
3.861LeuArg: 3.861 ± 0.376
5.068LeuSer: 5.068 ± 0.444
4.413LeuThr: 4.413 ± 0.389
5.033LeuVal: 5.033 ± 0.503
0.689LeuTrp: 0.689 ± 0.156
2.689LeuTyr: 2.689 ± 0.338
0.0LeuXaa: 0.0 ± 0.0
Met
2.034MetAla: 2.034 ± 0.297
0.345MetCys: 0.345 ± 0.109
1.207MetAsp: 1.207 ± 0.179
2.172MetGlu: 2.172 ± 0.313
0.862MetPhe: 0.862 ± 0.206
1.62MetGly: 1.62 ± 0.235
0.552MetHis: 0.552 ± 0.151
2.034MetIle: 2.034 ± 0.292
2.62MetLys: 2.62 ± 0.351
2.448MetLeu: 2.448 ± 0.388
0.621MetMet: 0.621 ± 0.153
1.241MetAsn: 1.241 ± 0.211
0.862MetPro: 0.862 ± 0.151
1.103MetGln: 1.103 ± 0.228
1.103MetArg: 1.103 ± 0.198
2.206MetSer: 2.206 ± 0.306
1.931MetThr: 1.931 ± 0.25
1.103MetVal: 1.103 ± 0.23
0.31MetTrp: 0.31 ± 0.124
1.034MetTyr: 1.034 ± 0.187
0.0MetXaa: 0.0 ± 0.0
Asn
3.827AsnAla: 3.827 ± 0.442
0.552AsnCys: 0.552 ± 0.154
2.93AsnAsp: 2.93 ± 0.373
2.689AsnGlu: 2.689 ± 0.281
2.068AsnPhe: 2.068 ± 0.281
4.275AsnGly: 4.275 ± 0.543
0.862AsnHis: 0.862 ± 0.213
3.895AsnIle: 3.895 ± 0.372
3.516AsnLys: 3.516 ± 0.371
4.516AsnLeu: 4.516 ± 0.382
1.413AsnMet: 1.413 ± 0.184
2.275AsnAsn: 2.275 ± 0.294
2.31AsnPro: 2.31 ± 0.271
1.482AsnGln: 1.482 ± 0.18
2.068AsnArg: 2.068 ± 0.289
3.551AsnSer: 3.551 ± 0.355
3.034AsnThr: 3.034 ± 0.276
3.585AsnVal: 3.585 ± 0.365
0.689AsnTrp: 0.689 ± 0.167
1.62AsnTyr: 1.62 ± 0.269
0.0AsnXaa: 0.0 ± 0.0
Pro
2.275ProAla: 2.275 ± 0.305
0.31ProCys: 0.31 ± 0.11
2.344ProAsp: 2.344 ± 0.32
3.413ProGlu: 3.413 ± 0.406
1.586ProPhe: 1.586 ± 0.238
1.999ProGly: 1.999 ± 0.244
0.448ProHis: 0.448 ± 0.128
1.931ProIle: 1.931 ± 0.335
1.999ProLys: 1.999 ± 0.26
2.482ProLeu: 2.482 ± 0.287
0.621ProMet: 0.621 ± 0.135
2.379ProAsn: 2.379 ± 0.334
1.379ProPro: 1.379 ± 0.238
1.034ProGln: 1.034 ± 0.202
1.586ProArg: 1.586 ± 0.215
2.103ProSer: 2.103 ± 0.254
2.172ProThr: 2.172 ± 0.24
2.654ProVal: 2.654 ± 0.273
0.586ProTrp: 0.586 ± 0.132
1.551ProTyr: 1.551 ± 0.248
0.0ProXaa: 0.0 ± 0.0
Gln
3.103GlnAla: 3.103 ± 0.409
0.448GlnCys: 0.448 ± 0.123
2.034GlnAsp: 2.034 ± 0.299
2.482GlnGlu: 2.482 ± 0.368
1.862GlnPhe: 1.862 ± 0.254
2.068GlnGly: 2.068 ± 0.215
0.621GlnHis: 0.621 ± 0.162
2.068GlnIle: 2.068 ± 0.232
2.62GlnLys: 2.62 ± 0.369
3.827GlnLeu: 3.827 ± 0.432
0.793GlnMet: 0.793 ± 0.148
1.448GlnAsn: 1.448 ± 0.188
0.414GlnPro: 0.414 ± 0.126
1.793GlnGln: 1.793 ± 0.313
1.724GlnArg: 1.724 ± 0.251
2.482GlnSer: 2.482 ± 0.307
1.965GlnThr: 1.965 ± 0.26
2.93GlnVal: 2.93 ± 0.314
0.483GlnTrp: 0.483 ± 0.154
1.413GlnTyr: 1.413 ± 0.238
0.0GlnXaa: 0.0 ± 0.0
Arg
3.24ArgAla: 3.24 ± 0.384
0.207ArgCys: 0.207 ± 0.081
2.792ArgAsp: 2.792 ± 0.297
3.103ArgGlu: 3.103 ± 0.365
1.758ArgPhe: 1.758 ± 0.219
3.516ArgGly: 3.516 ± 0.308
0.552ArgHis: 0.552 ± 0.174
3.068ArgIle: 3.068 ± 0.322
2.723ArgLys: 2.723 ± 0.387
3.689ArgLeu: 3.689 ± 0.337
1.379ArgMet: 1.379 ± 0.244
2.448ArgAsn: 2.448 ± 0.307
1.276ArgPro: 1.276 ± 0.202
1.551ArgGln: 1.551 ± 0.221
2.275ArgArg: 2.275 ± 0.319
2.31ArgSer: 2.31 ± 0.276
2.551ArgThr: 2.551 ± 0.253
3.344ArgVal: 3.344 ± 0.299
0.379ArgTrp: 0.379 ± 0.129
1.758ArgTyr: 1.758 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
4.792SerAla: 4.792 ± 0.725
0.586SerCys: 0.586 ± 0.163
3.309SerAsp: 3.309 ± 0.366
3.723SerGlu: 3.723 ± 0.491
3.172SerPhe: 3.172 ± 0.33
4.55SerGly: 4.55 ± 0.423
0.793SerHis: 0.793 ± 0.156
4.585SerIle: 4.585 ± 0.476
4.413SerLys: 4.413 ± 0.391
5.654SerLeu: 5.654 ± 0.464
1.517SerMet: 1.517 ± 0.24
3.24SerAsn: 3.24 ± 0.318
1.965SerPro: 1.965 ± 0.282
1.586SerGln: 1.586 ± 0.256
2.517SerArg: 2.517 ± 0.344
4.413SerSer: 4.413 ± 0.373
3.689SerThr: 3.689 ± 0.367
4.068SerVal: 4.068 ± 0.354
1.241SerTrp: 1.241 ± 0.231
2.482SerTyr: 2.482 ± 0.291
0.0SerXaa: 0.0 ± 0.0
Thr
4.861ThrAla: 4.861 ± 0.598
0.483ThrCys: 0.483 ± 0.125
3.068ThrAsp: 3.068 ± 0.305
3.275ThrGlu: 3.275 ± 0.327
2.344ThrPhe: 2.344 ± 0.28
4.895ThrGly: 4.895 ± 0.464
1.0ThrHis: 1.0 ± 0.15
4.033ThrIle: 4.033 ± 0.36
3.895ThrLys: 3.895 ± 0.426
4.516ThrLeu: 4.516 ± 0.385
1.379ThrMet: 1.379 ± 0.21
3.723ThrAsn: 3.723 ± 0.441
2.585ThrPro: 2.585 ± 0.266
2.206ThrGln: 2.206 ± 0.275
2.62ThrArg: 2.62 ± 0.299
3.827ThrSer: 3.827 ± 0.444
2.999ThrThr: 2.999 ± 0.296
4.344ThrVal: 4.344 ± 0.432
0.552ThrTrp: 0.552 ± 0.135
2.068ThrTyr: 2.068 ± 0.259
0.0ThrXaa: 0.0 ± 0.0
Val
5.102ValAla: 5.102 ± 0.489
0.552ValCys: 0.552 ± 0.15
4.619ValAsp: 4.619 ± 0.461
4.482ValGlu: 4.482 ± 0.303
2.413ValPhe: 2.413 ± 0.275
3.964ValGly: 3.964 ± 0.375
0.862ValHis: 0.862 ± 0.179
4.482ValIle: 4.482 ± 0.404
4.792ValLys: 4.792 ± 0.358
4.999ValLeu: 4.999 ± 0.355
1.62ValMet: 1.62 ± 0.218
3.068ValAsn: 3.068 ± 0.389
2.62ValPro: 2.62 ± 0.242
2.585ValGln: 2.585 ± 0.357
2.654ValArg: 2.654 ± 0.316
3.964ValSer: 3.964 ± 0.407
4.585ValThr: 4.585 ± 0.433
4.171ValVal: 4.171 ± 0.569
0.483ValTrp: 0.483 ± 0.181
2.689ValTyr: 2.689 ± 0.269
0.0ValXaa: 0.0 ± 0.0
Trp
0.517TrpAla: 0.517 ± 0.153
0.172TrpCys: 0.172 ± 0.075
1.0TrpAsp: 1.0 ± 0.175
0.931TrpGlu: 0.931 ± 0.174
0.517TrpPhe: 0.517 ± 0.149
0.689TrpGly: 0.689 ± 0.134
0.172TrpHis: 0.172 ± 0.07
0.655TrpIle: 0.655 ± 0.175
0.896TrpLys: 0.896 ± 0.162
1.482TrpLeu: 1.482 ± 0.293
0.31TrpMet: 0.31 ± 0.105
0.517TrpAsn: 0.517 ± 0.149
0.207TrpPro: 0.207 ± 0.099
0.621TrpGln: 0.621 ± 0.147
0.517TrpArg: 0.517 ± 0.123
0.793TrpSer: 0.793 ± 0.2
0.689TrpThr: 0.689 ± 0.137
0.655TrpVal: 0.655 ± 0.161
0.207TrpTrp: 0.207 ± 0.089
0.345TrpTyr: 0.345 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.827TyrAla: 2.827 ± 0.309
0.655TyrCys: 0.655 ± 0.158
3.275TyrAsp: 3.275 ± 0.35
2.172TyrGlu: 2.172 ± 0.263
1.999TyrPhe: 1.999 ± 0.316
2.448TyrGly: 2.448 ± 0.337
1.207TyrHis: 1.207 ± 0.18
2.31TyrIle: 2.31 ± 0.268
2.551TyrLys: 2.551 ± 0.314
2.965TyrLeu: 2.965 ± 0.354
1.0TyrMet: 1.0 ± 0.21
2.172TyrAsn: 2.172 ± 0.242
1.172TyrPro: 1.172 ± 0.173
1.517TyrGln: 1.517 ± 0.227
1.517TyrArg: 1.517 ± 0.229
2.551TyrSer: 2.551 ± 0.289
2.413TyrThr: 2.413 ± 0.324
2.206TyrVal: 2.206 ± 0.298
0.414TyrTrp: 0.414 ± 0.129
1.31TyrTyr: 1.31 ± 0.247
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 149 proteins (29009 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski