Bacteroides finegoldii
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3498 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A173WGT6|A0A173WGT6_9BACE AraC-type DNA-binding domain-containing proteins OS=Bacteroides finegoldii OX=338188 GN=adaA_1 PE=4 SV=1
MM1 pKa = 7.64 WKK3 pKa = 10.38 FKK5 pKa = 10.58 FGLWAVVLITSVFSFSACGGDD26 pKa = 3.94 DD27 pKa = 4.55 DD28 pKa = 6.37 NDD30 pKa = 4.15 TFVPPANIEE39 pKa = 4.08 VALKK43 pKa = 10.17 QLYY46 pKa = 9.75 PGAQNIEE53 pKa = 4.09 WEE55 pKa = 4.63 VKK57 pKa = 9.15 GVYY60 pKa = 9.37 YY61 pKa = 10.83 VADD64 pKa = 3.53 CWVTGDD70 pKa = 4.16 EE71 pKa = 4.38 LEE73 pKa = 4.18 VWFDD77 pKa = 3.82 VNANWVMTEE86 pKa = 3.93 NEE88 pKa = 4.4 LDD90 pKa = 3.66 NVNQLVPAVYY100 pKa = 8.72 TAFMDD105 pKa = 3.78 SDD107 pKa = 4.3 YY108 pKa = 10.36 NTWGVTDD115 pKa = 4.33 VYY117 pKa = 11.56 VLTYY121 pKa = 9.45 PLNPTTSVIQVKK133 pKa = 10.17 QGSQRR138 pKa = 11.84 YY139 pKa = 8.41 SLYY142 pKa = 10.29 FSQEE146 pKa = 3.24 GGLLHH151 pKa = 6.83 EE152 pKa = 5.27 KK153 pKa = 10.45 NISNGDD159 pKa = 3.58 DD160 pKa = 3.82 TNWPPTEE167 pKa = 3.83
Molecular weight: 18.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.808
Bjellqvist 4.012
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.139
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.935
Patrickios 0.477
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|A0A174BS10|A0A174BS10_9BACE Uncharacterized protein OS=Bacteroides finegoldii OX=338188 GN=ERS852397_01232 PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 4.29 EE3 pKa = 4.94 AVIKK7 pKa = 9.96 QIQRR11 pKa = 11.84 EE12 pKa = 4.28 GADD15 pKa = 3.29 ALLDD19 pKa = 3.49 IGVSVPLKK27 pKa = 10.3 AFHH30 pKa = 6.66 IPFRR34 pKa = 11.84 KK35 pKa = 9.79 SPLEE39 pKa = 3.57 LRR41 pKa = 11.84 VTMRR45 pKa = 11.84 RR46 pKa = 11.84 PYY48 pKa = 10.13 MSGQILFARR57 pKa = 11.84 TYY59 pKa = 10.37 LSMGITSEE67 pKa = 4.12 EE68 pKa = 3.51 MWGFSKK74 pKa = 10.86 EE75 pKa = 3.87 EE76 pKa = 3.83 EE77 pKa = 4.22 MQFLASHH84 pKa = 6.52 GKK86 pKa = 9.35 AVSRR90 pKa = 11.84 MVAYY94 pKa = 7.47 TLCRR98 pKa = 11.84 GPFSRR103 pKa = 11.84 RR104 pKa = 11.84 VLLRR108 pKa = 11.84 PVAWLIRR115 pKa = 11.84 NFMEE119 pKa = 3.96 QRR121 pKa = 11.84 YY122 pKa = 8.63 LVGAIKK128 pKa = 10.52 RR129 pKa = 11.84 FVSLMGTDD137 pKa = 3.43 PFIPIIRR144 pKa = 11.84 SAEE147 pKa = 3.87 RR148 pKa = 11.84 TNPMSLRR155 pKa = 11.84 LSQRR159 pKa = 11.84 KK160 pKa = 9.27 KK161 pKa = 10.83 GSS163 pKa = 3.15
Molecular weight: 18.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.692
IPC_protein 10.57
Toseland 10.745
ProMoST 10.467
Dawson 10.833
Bjellqvist 10.555
Wikipedia 11.067
Rodwell 11.008
Grimsley 10.877
Solomon 10.979
Lehninger 10.935
Nozaki 10.73
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.745
IPC_peptide 10.979
IPC2_peptide 9.472
IPC2.peptide.svr19 8.658
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3498
0
3498
1331831
29
2073
380.7
43.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.924 ± 0.039
1.202 ± 0.016
5.538 ± 0.027
6.69 ± 0.039
4.535 ± 0.029
6.853 ± 0.038
1.798 ± 0.015
6.923 ± 0.037
6.815 ± 0.029
8.957 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.735 ± 0.019
5.255 ± 0.032
3.77 ± 0.019
3.428 ± 0.024
4.491 ± 0.03
6.132 ± 0.033
5.663 ± 0.027
6.394 ± 0.027
1.33 ± 0.017
4.568 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here