Hubei coleoptera virus 3
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KMZ8|A0A1L3KMZ8_9VIRU Putative glycoprotein OS=Hubei coleoptera virus 3 OX=1922862 PE=4 SV=1
MM1 pKa = 7.16 FPSRR5 pKa = 11.84 QRR7 pKa = 11.84 VQLAMNRR14 pKa = 11.84 GRR16 pKa = 11.84 TIRR19 pKa = 11.84 ASPMFAAIGQFSKK32 pKa = 11.02 SVDD35 pKa = 2.9 IGRR38 pKa = 11.84 EE39 pKa = 3.96 LLLMKK44 pKa = 10.73 AMSRR48 pKa = 11.84 INNQPGFVNFSGAILIANQLSKK70 pKa = 10.52 IVSSPTGAVTGSTSISQVSGILFARR95 pKa = 11.84 WMLTEE100 pKa = 4.54 EE101 pKa = 4.17 LTEE104 pKa = 4.1 NDD106 pKa = 3.13 IQSSRR111 pKa = 11.84 KK112 pKa = 9.71 EE113 pKa = 3.82 IVRR116 pKa = 11.84 LLDD119 pKa = 3.62 LQPPLSGPDD128 pKa = 3.46 VPNLLVKK135 pKa = 10.65 IMIEE139 pKa = 3.48 IGKK142 pKa = 9.21 IPKK145 pKa = 10.12 DD146 pKa = 3.67 SIIDD150 pKa = 3.55 HH151 pKa = 7.29 DD152 pKa = 4.33 GATARR157 pKa = 11.84 LGRR160 pKa = 11.84 FSCEE164 pKa = 3.15 AVIPQEE170 pKa = 3.76 GLAARR175 pKa = 11.84 KK176 pKa = 9.57 VVFRR180 pKa = 11.84 EE181 pKa = 3.65 YY182 pKa = 10.41 RR183 pKa = 11.84 GNLIPTTWWGHH194 pKa = 3.97 QTNSEE199 pKa = 4.27 CANEE203 pKa = 4.26 LSTLTTGPTTLDD215 pKa = 3.7 FDD217 pKa = 5.24 SSLQPMTPDD226 pKa = 3.64 TPWASLTDD234 pKa = 4.06 DD235 pKa = 3.98 DD236 pKa = 5.6 AKK238 pKa = 11.25 LGMVEE243 pKa = 4.83 LYY245 pKa = 10.99 GSIHH249 pKa = 5.91 SEE251 pKa = 3.63 RR252 pKa = 11.84 MRR254 pKa = 11.84 QIFNSAFVIAVIALSKK270 pKa = 9.69 HH271 pKa = 6.83 DD272 pKa = 4.33 NMTDD276 pKa = 2.67 SWLEE280 pKa = 3.78 TRR282 pKa = 11.84 LEE284 pKa = 3.98 RR285 pKa = 11.84 LVTCLDD291 pKa = 3.88 DD292 pKa = 4.18 SQMTTTVTSEE302 pKa = 4.17 AIKK305 pKa = 10.65 NFYY308 pKa = 10.19 RR309 pKa = 11.84 RR310 pKa = 11.84 YY311 pKa = 8.24 QVEE314 pKa = 4.35 KK315 pKa = 10.62 IKK317 pKa = 11.12 VDD319 pKa = 3.59 DD320 pKa = 4.81 LYY322 pKa = 11.75 DD323 pKa = 3.75 LFVYY327 pKa = 10.41 INSLCLDD334 pKa = 4.16 FDD336 pKa = 4.86 LEE338 pKa = 4.21 PMQWILEE345 pKa = 4.09 QAFVNNVASATAIAEE360 pKa = 4.31 IIAKK364 pKa = 10.04 SKK366 pKa = 8.95 TVPITTILEE375 pKa = 4.26 TLPATQIQSLITLVAHH391 pKa = 6.56 LQHH394 pKa = 7.3 DD395 pKa = 4.66 PFCAIIRR402 pKa = 11.84 PPITVRR408 pKa = 11.84 QYY410 pKa = 11.2 PDD412 pKa = 2.77 VAYY415 pKa = 10.77 VGIAATYY422 pKa = 10.03 NSRR425 pKa = 11.84 TGPGGKK431 pKa = 9.08 RR432 pKa = 11.84 YY433 pKa = 9.43 QGKK436 pKa = 8.63 PDD438 pKa = 3.28 TMATLTTAALNGLVNFLLQTSRR460 pKa = 11.84 TQMEE464 pKa = 4.27 MEE466 pKa = 4.94 LNLDD470 pKa = 3.99 TYY472 pKa = 11.32 AKK474 pKa = 9.72 KK475 pKa = 10.45 FLMGQDD481 pKa = 3.08 NSQVVFIDD489 pKa = 2.95 QDD491 pKa = 4.19 YY492 pKa = 10.76 YY493 pKa = 11.12 VIKK496 pKa = 9.95 PDD498 pKa = 3.85 VVQQPTAGQSGTSTTPTQTDD518 pKa = 3.65 VEE520 pKa = 4.28 ISRR523 pKa = 11.84 DD524 pKa = 3.68 LKK526 pKa = 11.15 DD527 pKa = 3.15 QLLRR531 pKa = 11.84 SEE533 pKa = 4.18 RR534 pKa = 11.84 ADD536 pKa = 2.98 WPRR539 pKa = 11.84 RR540 pKa = 11.84 AKK542 pKa = 10.25 SLPEE546 pKa = 3.53 GTVRR550 pKa = 11.84 MTQQEE555 pKa = 4.42 VFAQIVDD562 pKa = 3.84 KK563 pKa = 11.21 EE564 pKa = 4.29 PVKK567 pKa = 10.64 MKK569 pKa = 10.67 AFRR572 pKa = 11.84 DD573 pKa = 3.7 AMDD576 pKa = 3.64 ILLDD580 pKa = 3.4 ITQQKK585 pKa = 9.09 PISVLTGTNATARR598 pKa = 11.84 KK599 pKa = 8.61 RR600 pKa = 11.84 KK601 pKa = 9.57 SKK603 pKa = 10.04 ISGRR607 pKa = 11.84 MRR609 pKa = 11.84 EE610 pKa = 4.52 IIVSWGVTMEE620 pKa = 4.72 EE621 pKa = 3.47 IWNTEE626 pKa = 4.0 DD627 pKa = 3.97 APADD631 pKa = 3.94 LTHH634 pKa = 5.96 GTIDD638 pKa = 3.43 IDD640 pKa = 3.84 EE641 pKa = 4.43 LVCLGRR647 pKa = 11.84 KK648 pKa = 9.23 KK649 pKa = 10.43 IGVAGAQGPAQAPGPAEE666 pKa = 3.91 VVEE669 pKa = 4.22 QANAA673 pKa = 3.38
Molecular weight: 74.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.458
IPC2_protein 5.486
IPC_protein 5.461
Toseland 5.55
ProMoST 5.677
Dawson 5.537
Bjellqvist 5.639
Wikipedia 5.461
Rodwell 5.474
Grimsley 5.55
Solomon 5.537
Lehninger 5.512
Nozaki 5.715
DTASelect 5.893
Thurlkill 5.639
EMBOSS 5.614
Sillero 5.792
Patrickios 4.482
IPC_peptide 5.55
IPC2_peptide 5.779
IPC2.peptide.svr19 5.847
Protein with the highest isoelectric point:
>tr|A0A1L3KMZ8|A0A1L3KMZ8_9VIRU Putative glycoprotein OS=Hubei coleoptera virus 3 OX=1922862 PE=4 SV=1
MM1 pKa = 7.32 FFAILLAILTLSYY14 pKa = 11.02 GSPTGRR20 pKa = 11.84 SKK22 pKa = 11.59 LNLEE26 pKa = 4.33 TATVMTQHH34 pKa = 7.27 DD35 pKa = 4.94 PMFLPYY41 pKa = 10.15 FEE43 pKa = 4.89 WKK45 pKa = 10.18 HH46 pKa = 5.4 LLHH49 pKa = 6.38 VQLLCPDD56 pKa = 4.03 SNIMSLIDD64 pKa = 3.82 ALTKK68 pKa = 10.58 NVYY71 pKa = 10.26 DD72 pKa = 3.55 QTNKK76 pKa = 10.72 LEE78 pKa = 4.78 LNLQVLTEE86 pKa = 4.13 EE87 pKa = 5.22 LSKK90 pKa = 11.6 DD91 pKa = 3.51 MNNNTLSTNTLLMRR105 pKa = 11.84 DD106 pKa = 3.63 QVHH109 pKa = 5.5 VAKK112 pKa = 10.64 NLQAFLRR119 pKa = 11.84 GTSEE123 pKa = 3.5 KK124 pKa = 9.59 WKK126 pKa = 10.57 KK127 pKa = 9.95 LVNGLFKK134 pKa = 10.56 FHH136 pKa = 6.64 GMAQQSTCYY145 pKa = 10.26 HH146 pKa = 5.98 LVKK149 pKa = 9.93 TNQGKK154 pKa = 7.1 TVPTGISGMASDD166 pKa = 5.25 ASTSYY171 pKa = 10.92 EE172 pKa = 3.73 GDD174 pKa = 3.04 LWKK177 pKa = 10.75 VLHH180 pKa = 7.5 DD181 pKa = 4.05 SMTKK185 pKa = 9.78 SRR187 pKa = 11.84 QKK189 pKa = 9.83 RR190 pKa = 11.84 TIHH193 pKa = 5.67 IHH195 pKa = 6.25 GSDD198 pKa = 2.8 NTIFHH203 pKa = 7.06 NSQITKK209 pKa = 9.93 PSDD212 pKa = 2.86 ITKK215 pKa = 10.05 EE216 pKa = 3.81 LRR218 pKa = 11.84 RR219 pKa = 11.84 DD220 pKa = 3.27 KK221 pKa = 11.0 DD222 pKa = 3.56 LSDD225 pKa = 3.71 NPTTVKK231 pKa = 10.34 SVSYY235 pKa = 9.95 HH236 pKa = 5.21 NPKK239 pKa = 10.0 SSEE242 pKa = 3.86 MLKK245 pKa = 10.51 LQNCFCWDD253 pKa = 3.33 EE254 pKa = 4.21 ATQQAHH260 pKa = 5.6 VDD262 pKa = 4.06 RR263 pKa = 11.84 NCDD266 pKa = 3.2 LANCKK271 pKa = 9.68 RR272 pKa = 11.84 VLDD275 pKa = 3.63 KK276 pKa = 10.92 WLMKK280 pKa = 10.8 GFGQITGNVKK290 pKa = 10.5 LYY292 pKa = 10.7 NEE294 pKa = 4.14 YY295 pKa = 10.66 GKK297 pKa = 8.21 TVIKK301 pKa = 8.19 TTTQPPSQVEE311 pKa = 4.28 DD312 pKa = 3.82 EE313 pKa = 4.81 VVGSPDD319 pKa = 2.93 QSEE322 pKa = 4.51 VSRR325 pKa = 11.84 EE326 pKa = 3.61 VDD328 pKa = 3.4 GQAEE332 pKa = 4.13 TLAEE336 pKa = 4.04 KK337 pKa = 10.62 LLRR340 pKa = 11.84 KK341 pKa = 9.94 HH342 pKa = 6.0 PVVPSKK348 pKa = 9.99 TMADD352 pKa = 3.29 ANQASRR358 pKa = 11.84 NLPTVDD364 pKa = 3.34 EE365 pKa = 4.34 QYY367 pKa = 11.37 EE368 pKa = 4.21 QILLEE373 pKa = 4.31 NYY375 pKa = 6.68 QTSAFVYY382 pKa = 7.35 QLCRR386 pKa = 11.84 LRR388 pKa = 11.84 LSDD391 pKa = 4.8 NLQNCLGTIPEE402 pKa = 4.34 TNALNHH408 pKa = 5.42 LWEE411 pKa = 4.96 RR412 pKa = 11.84 LQSTHH417 pKa = 5.58 SKK419 pKa = 10.04 RR420 pKa = 11.84 IKK422 pKa = 9.86 RR423 pKa = 11.84 SSNRR427 pKa = 11.84 VKK429 pKa = 10.55 RR430 pKa = 11.84 APLGIIGRR438 pKa = 11.84 LYY440 pKa = 10.13 KK441 pKa = 10.06 WLFGVATVDD450 pKa = 4.24 DD451 pKa = 4.03 VDD453 pKa = 3.56 EE454 pKa = 4.14 VRR456 pKa = 11.84 RR457 pKa = 11.84 NFVSYY462 pKa = 11.1 LKK464 pKa = 10.62 GQQNLSKK471 pKa = 10.81 ANQEE475 pKa = 3.91 FMTNQLSINQHH486 pKa = 5.13 FDD488 pKa = 3.35 EE489 pKa = 4.73 NLKK492 pKa = 10.32 ILNTKK497 pKa = 10.05 ISDD500 pKa = 3.79 TVKK503 pKa = 10.8 GMNSTIDD510 pKa = 3.37 MFMDD514 pKa = 5.39 TIQQTNNVFQDD525 pKa = 3.97 LYY527 pKa = 10.39 LTTKK531 pKa = 10.61 LSSLLIHH538 pKa = 6.4 GNILITGAVIDD549 pKa = 4.21 ALRR552 pKa = 11.84 INQYY556 pKa = 9.37 MDD558 pKa = 3.68 SLSSRR563 pKa = 11.84 YY564 pKa = 9.71 LSLYY568 pKa = 9.86 HH569 pKa = 7.18 ALLSDD574 pKa = 3.71 TLTAEE579 pKa = 4.14 ILDD582 pKa = 4.14 PASLKK587 pKa = 10.24 TILLEE592 pKa = 4.52 INNKK596 pKa = 9.62 LPPNYY601 pKa = 9.77 QVIDD605 pKa = 4.03 LPDD608 pKa = 3.44 FRR610 pKa = 11.84 SWYY613 pKa = 8.42 KK614 pKa = 10.35 VSYY617 pKa = 9.07 STVSLIDD624 pKa = 3.29 NSIVYY629 pKa = 9.89 AFKK632 pKa = 10.69 FPIVRR637 pKa = 11.84 KK638 pKa = 10.0 SSEE641 pKa = 3.61 PTLWHH646 pKa = 6.44 FNSLPVFKK654 pKa = 10.86 NGTAFKK660 pKa = 10.74 LNYY663 pKa = 8.43 HH664 pKa = 6.01 DD665 pKa = 3.37 HH666 pKa = 6.03 WVYY669 pKa = 11.14 LDD671 pKa = 3.38 EE672 pKa = 6.27 HH673 pKa = 5.37 NQEE676 pKa = 4.33 WVPMSQTDD684 pKa = 3.56 FNARR688 pKa = 11.84 CDD690 pKa = 3.59 KK691 pKa = 10.14 STSVCSGFASRR702 pKa = 11.84 YY703 pKa = 7.2 TYY705 pKa = 11.19 SKK707 pKa = 11.31 NNYY710 pKa = 6.96 NHH712 pKa = 6.99 CLLSLLYY719 pKa = 10.45 DD720 pKa = 3.49 SSKK723 pKa = 11.37 VIDD726 pKa = 3.85 QCEE729 pKa = 4.01 MSPIDD734 pKa = 3.78 IDD736 pKa = 3.93 QVLDD740 pKa = 4.25 PIIAISIPPNQWLVEE755 pKa = 3.84 TLYY758 pKa = 10.28 PVKK761 pKa = 10.36 AVKK764 pKa = 10.16 SCPAKK769 pKa = 10.3 SGQFITDD776 pKa = 3.01 SVMISGVMTIEE787 pKa = 4.58 LAPLCKK793 pKa = 9.75 ISIAEE798 pKa = 4.11 LTLEE802 pKa = 4.61 SSLAPPGLEE811 pKa = 4.0 SQSYY815 pKa = 8.62 APEE818 pKa = 3.94 YY819 pKa = 9.39 TLEE822 pKa = 4.14 SVKK825 pKa = 10.52 ISTSDD830 pKa = 2.85 IDD832 pKa = 4.65 IIALKK837 pKa = 9.56 EE838 pKa = 4.11 NKK840 pKa = 9.37 VPQIDD845 pKa = 3.23 IKK847 pKa = 10.38 MDD849 pKa = 2.91 KK850 pKa = 9.7 STWNTKK856 pKa = 9.44 AMQIKK861 pKa = 9.67 IKK863 pKa = 10.72 SVEE866 pKa = 4.27 TNLNAQKK873 pKa = 10.54 EE874 pKa = 4.41 LVSNISEE881 pKa = 4.2 INKK884 pKa = 9.59 KK885 pKa = 9.89 LQADD889 pKa = 4.76 LIKK892 pKa = 10.34 IEE894 pKa = 4.23 SSSGKK899 pKa = 8.16 TFSSQWISFYY909 pKa = 10.1 WEE911 pKa = 3.91 NIIFYY916 pKa = 9.15 IWVIILTLMTLRR928 pKa = 11.84 VYY930 pKa = 10.85 VRR932 pKa = 11.84 VFGIPLIGRR941 pKa = 11.84 GNGIMAKK948 pKa = 8.96 ATFPLVSSSGFGTANAFPPQGKK970 pKa = 9.22 IYY972 pKa = 9.82 MDD974 pKa = 4.74 GSTIWASAPNATSNVMVNVTNKK996 pKa = 9.61 FNEE999 pKa = 4.71 FFSQPHH1005 pKa = 5.28 NTTSVIHH1012 pKa = 5.97 LCVMVALMLLMLILMYY1028 pKa = 11.19 SLMCHH1033 pKa = 5.64 HH1034 pKa = 6.91 QSMLLRR1040 pKa = 11.84 KK1041 pKa = 9.13 LLSLGVLPINTRR1053 pKa = 11.84 EE1054 pKa = 4.02 SQHH1057 pKa = 7.4 IGEE1060 pKa = 5.17 IGVQLYY1066 pKa = 9.86 VLVMMKK1072 pKa = 10.79 SMFGSKK1078 pKa = 7.15 THH1080 pKa = 5.45 YY1081 pKa = 10.85 AEE1083 pKa = 5.56 VGIQMCTLPGNIRR1096 pKa = 11.84 DD1097 pKa = 4.45 WICTSNLSEE1106 pKa = 4.05 NKK1108 pKa = 9.33 TFIVPKK1114 pKa = 9.41 WVCRR1118 pKa = 11.84 WSSTLEE1124 pKa = 4.41 LSCQWGPICLKK1135 pKa = 8.65 STKK1138 pKa = 9.91 YY1139 pKa = 10.71 PNVDD1143 pKa = 3.08 TCKK1146 pKa = 10.43 DD1147 pKa = 3.72 LPKK1150 pKa = 10.46 HH1151 pKa = 5.97 GSFNMRR1157 pKa = 11.84 EE1158 pKa = 4.28 VIEE1161 pKa = 4.33 QINPKK1166 pKa = 10.34 CPFIWKK1172 pKa = 9.44 YY1173 pKa = 10.12 ISAVEE1178 pKa = 4.05 VNKK1181 pKa = 10.43 VILRR1185 pKa = 11.84 THH1187 pKa = 6.43 NDD1189 pKa = 2.84 YY1190 pKa = 11.47 KK1191 pKa = 10.87 EE1192 pKa = 4.1 IYY1194 pKa = 9.53 SYY1196 pKa = 11.79 NSDD1199 pKa = 3.64 TLSS1202 pKa = 3.13
Molecular weight: 136.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.932
IPC2_protein 7.293
IPC_protein 7.117
Toseland 6.927
ProMoST 7.717
Dawson 7.819
Bjellqvist 8.214
Wikipedia 7.746
Rodwell 7.849
Grimsley 7.029
Solomon 7.849
Lehninger 7.863
Nozaki 8.434
DTASelect 7.98
Thurlkill 8.039
EMBOSS 8.053
Sillero 8.346
Patrickios 4.177
IPC_peptide 7.849
IPC2_peptide 7.263
IPC2.peptide.svr19 7.274
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
4334
673
2459
1444.7
164.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.73 ± 0.939
1.454 ± 0.205
5.653 ± 0.267
5.03 ± 0.207
4.199 ± 0.553
4.43 ± 0.33
2.653 ± 0.492
7.176 ± 0.155
5.699 ± 0.49
11.237 ± 0.828
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.276 ± 0.048
5.538 ± 0.597
4.522 ± 0.222
3.992 ± 0.678
4.569 ± 0.583
7.845 ± 0.633
6.714 ± 0.631
6.161 ± 0.212
1.777 ± 0.125
3.346 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here