Hubei coleoptera virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Jingchuvirales; Aliusviridae; Ollusvirus; Ollusvirus coleopteri

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KMZ8|A0A1L3KMZ8_9VIRU Putative glycoprotein OS=Hubei coleoptera virus 3 OX=1922862 PE=4 SV=1
MM1 pKa = 7.16FPSRR5 pKa = 11.84QRR7 pKa = 11.84VQLAMNRR14 pKa = 11.84GRR16 pKa = 11.84TIRR19 pKa = 11.84ASPMFAAIGQFSKK32 pKa = 11.02SVDD35 pKa = 2.9IGRR38 pKa = 11.84EE39 pKa = 3.96LLLMKK44 pKa = 10.73AMSRR48 pKa = 11.84INNQPGFVNFSGAILIANQLSKK70 pKa = 10.52IVSSPTGAVTGSTSISQVSGILFARR95 pKa = 11.84WMLTEE100 pKa = 4.54EE101 pKa = 4.17LTEE104 pKa = 4.1NDD106 pKa = 3.13IQSSRR111 pKa = 11.84KK112 pKa = 9.71EE113 pKa = 3.82IVRR116 pKa = 11.84LLDD119 pKa = 3.62LQPPLSGPDD128 pKa = 3.46VPNLLVKK135 pKa = 10.65IMIEE139 pKa = 3.48IGKK142 pKa = 9.21IPKK145 pKa = 10.12DD146 pKa = 3.67SIIDD150 pKa = 3.55HH151 pKa = 7.29DD152 pKa = 4.33GATARR157 pKa = 11.84LGRR160 pKa = 11.84FSCEE164 pKa = 3.15AVIPQEE170 pKa = 3.76GLAARR175 pKa = 11.84KK176 pKa = 9.57VVFRR180 pKa = 11.84EE181 pKa = 3.65YY182 pKa = 10.41RR183 pKa = 11.84GNLIPTTWWGHH194 pKa = 3.97QTNSEE199 pKa = 4.27CANEE203 pKa = 4.26LSTLTTGPTTLDD215 pKa = 3.7FDD217 pKa = 5.24SSLQPMTPDD226 pKa = 3.64TPWASLTDD234 pKa = 4.06DD235 pKa = 3.98DD236 pKa = 5.6AKK238 pKa = 11.25LGMVEE243 pKa = 4.83LYY245 pKa = 10.99GSIHH249 pKa = 5.91SEE251 pKa = 3.63RR252 pKa = 11.84MRR254 pKa = 11.84QIFNSAFVIAVIALSKK270 pKa = 9.69HH271 pKa = 6.83DD272 pKa = 4.33NMTDD276 pKa = 2.67SWLEE280 pKa = 3.78TRR282 pKa = 11.84LEE284 pKa = 3.98RR285 pKa = 11.84LVTCLDD291 pKa = 3.88DD292 pKa = 4.18SQMTTTVTSEE302 pKa = 4.17AIKK305 pKa = 10.65NFYY308 pKa = 10.19RR309 pKa = 11.84RR310 pKa = 11.84YY311 pKa = 8.24QVEE314 pKa = 4.35KK315 pKa = 10.62IKK317 pKa = 11.12VDD319 pKa = 3.59DD320 pKa = 4.81LYY322 pKa = 11.75DD323 pKa = 3.75LFVYY327 pKa = 10.41INSLCLDD334 pKa = 4.16FDD336 pKa = 4.86LEE338 pKa = 4.21PMQWILEE345 pKa = 4.09QAFVNNVASATAIAEE360 pKa = 4.31IIAKK364 pKa = 10.04SKK366 pKa = 8.95TVPITTILEE375 pKa = 4.26TLPATQIQSLITLVAHH391 pKa = 6.56LQHH394 pKa = 7.3DD395 pKa = 4.66PFCAIIRR402 pKa = 11.84PPITVRR408 pKa = 11.84QYY410 pKa = 11.2PDD412 pKa = 2.77VAYY415 pKa = 10.77VGIAATYY422 pKa = 10.03NSRR425 pKa = 11.84TGPGGKK431 pKa = 9.08RR432 pKa = 11.84YY433 pKa = 9.43QGKK436 pKa = 8.63PDD438 pKa = 3.28TMATLTTAALNGLVNFLLQTSRR460 pKa = 11.84TQMEE464 pKa = 4.27MEE466 pKa = 4.94LNLDD470 pKa = 3.99TYY472 pKa = 11.32AKK474 pKa = 9.72KK475 pKa = 10.45FLMGQDD481 pKa = 3.08NSQVVFIDD489 pKa = 2.95QDD491 pKa = 4.19YY492 pKa = 10.76YY493 pKa = 11.12VIKK496 pKa = 9.95PDD498 pKa = 3.85VVQQPTAGQSGTSTTPTQTDD518 pKa = 3.65VEE520 pKa = 4.28ISRR523 pKa = 11.84DD524 pKa = 3.68LKK526 pKa = 11.15DD527 pKa = 3.15QLLRR531 pKa = 11.84SEE533 pKa = 4.18RR534 pKa = 11.84ADD536 pKa = 2.98WPRR539 pKa = 11.84RR540 pKa = 11.84AKK542 pKa = 10.25SLPEE546 pKa = 3.53GTVRR550 pKa = 11.84MTQQEE555 pKa = 4.42VFAQIVDD562 pKa = 3.84KK563 pKa = 11.21EE564 pKa = 4.29PVKK567 pKa = 10.64MKK569 pKa = 10.67AFRR572 pKa = 11.84DD573 pKa = 3.7AMDD576 pKa = 3.64ILLDD580 pKa = 3.4ITQQKK585 pKa = 9.09PISVLTGTNATARR598 pKa = 11.84KK599 pKa = 8.61RR600 pKa = 11.84KK601 pKa = 9.57SKK603 pKa = 10.04ISGRR607 pKa = 11.84MRR609 pKa = 11.84EE610 pKa = 4.52IIVSWGVTMEE620 pKa = 4.72EE621 pKa = 3.47IWNTEE626 pKa = 4.0DD627 pKa = 3.97APADD631 pKa = 3.94LTHH634 pKa = 5.96GTIDD638 pKa = 3.43IDD640 pKa = 3.84EE641 pKa = 4.43LVCLGRR647 pKa = 11.84KK648 pKa = 9.23KK649 pKa = 10.43IGVAGAQGPAQAPGPAEE666 pKa = 3.91VVEE669 pKa = 4.22QANAA673 pKa = 3.38

Molecular weight:
74.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KMZ8|A0A1L3KMZ8_9VIRU Putative glycoprotein OS=Hubei coleoptera virus 3 OX=1922862 PE=4 SV=1
MM1 pKa = 7.32FFAILLAILTLSYY14 pKa = 11.02GSPTGRR20 pKa = 11.84SKK22 pKa = 11.59LNLEE26 pKa = 4.33TATVMTQHH34 pKa = 7.27DD35 pKa = 4.94PMFLPYY41 pKa = 10.15FEE43 pKa = 4.89WKK45 pKa = 10.18HH46 pKa = 5.4LLHH49 pKa = 6.38VQLLCPDD56 pKa = 4.03SNIMSLIDD64 pKa = 3.82ALTKK68 pKa = 10.58NVYY71 pKa = 10.26DD72 pKa = 3.55QTNKK76 pKa = 10.72LEE78 pKa = 4.78LNLQVLTEE86 pKa = 4.13EE87 pKa = 5.22LSKK90 pKa = 11.6DD91 pKa = 3.51MNNNTLSTNTLLMRR105 pKa = 11.84DD106 pKa = 3.63QVHH109 pKa = 5.5VAKK112 pKa = 10.64NLQAFLRR119 pKa = 11.84GTSEE123 pKa = 3.5KK124 pKa = 9.59WKK126 pKa = 10.57KK127 pKa = 9.95LVNGLFKK134 pKa = 10.56FHH136 pKa = 6.64GMAQQSTCYY145 pKa = 10.26HH146 pKa = 5.98LVKK149 pKa = 9.93TNQGKK154 pKa = 7.1TVPTGISGMASDD166 pKa = 5.25ASTSYY171 pKa = 10.92EE172 pKa = 3.73GDD174 pKa = 3.04LWKK177 pKa = 10.75VLHH180 pKa = 7.5DD181 pKa = 4.05SMTKK185 pKa = 9.78SRR187 pKa = 11.84QKK189 pKa = 9.83RR190 pKa = 11.84TIHH193 pKa = 5.67IHH195 pKa = 6.25GSDD198 pKa = 2.8NTIFHH203 pKa = 7.06NSQITKK209 pKa = 9.93PSDD212 pKa = 2.86ITKK215 pKa = 10.05EE216 pKa = 3.81LRR218 pKa = 11.84RR219 pKa = 11.84DD220 pKa = 3.27KK221 pKa = 11.0DD222 pKa = 3.56LSDD225 pKa = 3.71NPTTVKK231 pKa = 10.34SVSYY235 pKa = 9.95HH236 pKa = 5.21NPKK239 pKa = 10.0SSEE242 pKa = 3.86MLKK245 pKa = 10.51LQNCFCWDD253 pKa = 3.33EE254 pKa = 4.21ATQQAHH260 pKa = 5.6VDD262 pKa = 4.06RR263 pKa = 11.84NCDD266 pKa = 3.2LANCKK271 pKa = 9.68RR272 pKa = 11.84VLDD275 pKa = 3.63KK276 pKa = 10.92WLMKK280 pKa = 10.8GFGQITGNVKK290 pKa = 10.5LYY292 pKa = 10.7NEE294 pKa = 4.14YY295 pKa = 10.66GKK297 pKa = 8.21TVIKK301 pKa = 8.19TTTQPPSQVEE311 pKa = 4.28DD312 pKa = 3.82EE313 pKa = 4.81VVGSPDD319 pKa = 2.93QSEE322 pKa = 4.51VSRR325 pKa = 11.84EE326 pKa = 3.61VDD328 pKa = 3.4GQAEE332 pKa = 4.13TLAEE336 pKa = 4.04KK337 pKa = 10.62LLRR340 pKa = 11.84KK341 pKa = 9.94HH342 pKa = 6.0PVVPSKK348 pKa = 9.99TMADD352 pKa = 3.29ANQASRR358 pKa = 11.84NLPTVDD364 pKa = 3.34EE365 pKa = 4.34QYY367 pKa = 11.37EE368 pKa = 4.21QILLEE373 pKa = 4.31NYY375 pKa = 6.68QTSAFVYY382 pKa = 7.35QLCRR386 pKa = 11.84LRR388 pKa = 11.84LSDD391 pKa = 4.8NLQNCLGTIPEE402 pKa = 4.34TNALNHH408 pKa = 5.42LWEE411 pKa = 4.96RR412 pKa = 11.84LQSTHH417 pKa = 5.58SKK419 pKa = 10.04RR420 pKa = 11.84IKK422 pKa = 9.86RR423 pKa = 11.84SSNRR427 pKa = 11.84VKK429 pKa = 10.55RR430 pKa = 11.84APLGIIGRR438 pKa = 11.84LYY440 pKa = 10.13KK441 pKa = 10.06WLFGVATVDD450 pKa = 4.24DD451 pKa = 4.03VDD453 pKa = 3.56EE454 pKa = 4.14VRR456 pKa = 11.84RR457 pKa = 11.84NFVSYY462 pKa = 11.1LKK464 pKa = 10.62GQQNLSKK471 pKa = 10.81ANQEE475 pKa = 3.91FMTNQLSINQHH486 pKa = 5.13FDD488 pKa = 3.35EE489 pKa = 4.73NLKK492 pKa = 10.32ILNTKK497 pKa = 10.05ISDD500 pKa = 3.79TVKK503 pKa = 10.8GMNSTIDD510 pKa = 3.37MFMDD514 pKa = 5.39TIQQTNNVFQDD525 pKa = 3.97LYY527 pKa = 10.39LTTKK531 pKa = 10.61LSSLLIHH538 pKa = 6.4GNILITGAVIDD549 pKa = 4.21ALRR552 pKa = 11.84INQYY556 pKa = 9.37MDD558 pKa = 3.68SLSSRR563 pKa = 11.84YY564 pKa = 9.71LSLYY568 pKa = 9.86HH569 pKa = 7.18ALLSDD574 pKa = 3.71TLTAEE579 pKa = 4.14ILDD582 pKa = 4.14PASLKK587 pKa = 10.24TILLEE592 pKa = 4.52INNKK596 pKa = 9.62LPPNYY601 pKa = 9.77QVIDD605 pKa = 4.03LPDD608 pKa = 3.44FRR610 pKa = 11.84SWYY613 pKa = 8.42KK614 pKa = 10.35VSYY617 pKa = 9.07STVSLIDD624 pKa = 3.29NSIVYY629 pKa = 9.89AFKK632 pKa = 10.69FPIVRR637 pKa = 11.84KK638 pKa = 10.0SSEE641 pKa = 3.61PTLWHH646 pKa = 6.44FNSLPVFKK654 pKa = 10.86NGTAFKK660 pKa = 10.74LNYY663 pKa = 8.43HH664 pKa = 6.01DD665 pKa = 3.37HH666 pKa = 6.03WVYY669 pKa = 11.14LDD671 pKa = 3.38EE672 pKa = 6.27HH673 pKa = 5.37NQEE676 pKa = 4.33WVPMSQTDD684 pKa = 3.56FNARR688 pKa = 11.84CDD690 pKa = 3.59KK691 pKa = 10.14STSVCSGFASRR702 pKa = 11.84YY703 pKa = 7.2TYY705 pKa = 11.19SKK707 pKa = 11.31NNYY710 pKa = 6.96NHH712 pKa = 6.99CLLSLLYY719 pKa = 10.45DD720 pKa = 3.49SSKK723 pKa = 11.37VIDD726 pKa = 3.85QCEE729 pKa = 4.01MSPIDD734 pKa = 3.78IDD736 pKa = 3.93QVLDD740 pKa = 4.25PIIAISIPPNQWLVEE755 pKa = 3.84TLYY758 pKa = 10.28PVKK761 pKa = 10.36AVKK764 pKa = 10.16SCPAKK769 pKa = 10.3SGQFITDD776 pKa = 3.01SVMISGVMTIEE787 pKa = 4.58LAPLCKK793 pKa = 9.75ISIAEE798 pKa = 4.11LTLEE802 pKa = 4.61SSLAPPGLEE811 pKa = 4.0SQSYY815 pKa = 8.62APEE818 pKa = 3.94YY819 pKa = 9.39TLEE822 pKa = 4.14SVKK825 pKa = 10.52ISTSDD830 pKa = 2.85IDD832 pKa = 4.65IIALKK837 pKa = 9.56EE838 pKa = 4.11NKK840 pKa = 9.37VPQIDD845 pKa = 3.23IKK847 pKa = 10.38MDD849 pKa = 2.91KK850 pKa = 9.7STWNTKK856 pKa = 9.44AMQIKK861 pKa = 9.67IKK863 pKa = 10.72SVEE866 pKa = 4.27TNLNAQKK873 pKa = 10.54EE874 pKa = 4.41LVSNISEE881 pKa = 4.2INKK884 pKa = 9.59KK885 pKa = 9.89LQADD889 pKa = 4.76LIKK892 pKa = 10.34IEE894 pKa = 4.23SSSGKK899 pKa = 8.16TFSSQWISFYY909 pKa = 10.1WEE911 pKa = 3.91NIIFYY916 pKa = 9.15IWVIILTLMTLRR928 pKa = 11.84VYY930 pKa = 10.85VRR932 pKa = 11.84VFGIPLIGRR941 pKa = 11.84GNGIMAKK948 pKa = 8.96ATFPLVSSSGFGTANAFPPQGKK970 pKa = 9.22IYY972 pKa = 9.82MDD974 pKa = 4.74GSTIWASAPNATSNVMVNVTNKK996 pKa = 9.61FNEE999 pKa = 4.71FFSQPHH1005 pKa = 5.28NTTSVIHH1012 pKa = 5.97LCVMVALMLLMLILMYY1028 pKa = 11.19SLMCHH1033 pKa = 5.64HH1034 pKa = 6.91QSMLLRR1040 pKa = 11.84KK1041 pKa = 9.13LLSLGVLPINTRR1053 pKa = 11.84EE1054 pKa = 4.02SQHH1057 pKa = 7.4IGEE1060 pKa = 5.17IGVQLYY1066 pKa = 9.86VLVMMKK1072 pKa = 10.79SMFGSKK1078 pKa = 7.15THH1080 pKa = 5.45YY1081 pKa = 10.85AEE1083 pKa = 5.56VGIQMCTLPGNIRR1096 pKa = 11.84DD1097 pKa = 4.45WICTSNLSEE1106 pKa = 4.05NKK1108 pKa = 9.33TFIVPKK1114 pKa = 9.41WVCRR1118 pKa = 11.84WSSTLEE1124 pKa = 4.41LSCQWGPICLKK1135 pKa = 8.65STKK1138 pKa = 9.91YY1139 pKa = 10.71PNVDD1143 pKa = 3.08TCKK1146 pKa = 10.43DD1147 pKa = 3.72LPKK1150 pKa = 10.46HH1151 pKa = 5.97GSFNMRR1157 pKa = 11.84EE1158 pKa = 4.28VIEE1161 pKa = 4.33QINPKK1166 pKa = 10.34CPFIWKK1172 pKa = 9.44YY1173 pKa = 10.12ISAVEE1178 pKa = 4.05VNKK1181 pKa = 10.43VILRR1185 pKa = 11.84THH1187 pKa = 6.43NDD1189 pKa = 2.84YY1190 pKa = 11.47KK1191 pKa = 10.87EE1192 pKa = 4.1IYY1194 pKa = 9.53SYY1196 pKa = 11.79NSDD1199 pKa = 3.64TLSS1202 pKa = 3.13

Molecular weight:
136.4 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

4334

673

2459

1444.7

164.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.73 ± 0.939

1.454 ± 0.205

5.653 ± 0.267

5.03 ± 0.207

4.199 ± 0.553

4.43 ± 0.33

2.653 ± 0.492

7.176 ± 0.155

5.699 ± 0.49

11.237 ± 0.828

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.276 ± 0.048

5.538 ± 0.597

4.522 ± 0.222

3.992 ± 0.678

4.569 ± 0.583

7.845 ± 0.633

6.714 ± 0.631

6.161 ± 0.212

1.777 ± 0.125

3.346 ± 0.405

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski