Aequorivita sp. H23M31
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3051 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410FZF5|A0A410FZF5_9FLAO Uncharacterized protein OS=Aequorivita sp. H23M31 OX=2494375 GN=EI546_01105 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.32 KK3 pKa = 8.41 ITLIMFALATSFVFAQSATNAEE25 pKa = 4.7 SILSQTSGAIGDD37 pKa = 4.09 GGVACGDD44 pKa = 3.46 QGAGTTGDD52 pKa = 3.27 NLYY55 pKa = 10.48 FRR57 pKa = 11.84 SYY59 pKa = 10.6 YY60 pKa = 10.57 LYY62 pKa = 10.26 DD63 pKa = 3.35 YY64 pKa = 10.1 GVRR67 pKa = 11.84 GIVQATGLEE76 pKa = 4.53 FFVSTATGSTALQVMVFDD94 pKa = 4.46 MEE96 pKa = 4.38 GFPGGFDD103 pKa = 3.45 ATNMPVPLASASLTVTSAQVGTMVRR128 pKa = 11.84 ADD130 pKa = 3.8 FDD132 pKa = 3.89 SPAIVDD138 pKa = 3.58 EE139 pKa = 4.3 DD140 pKa = 4.06 TTLVALVYY148 pKa = 8.9 EE149 pKa = 4.63 TDD151 pKa = 3.29 GQTAQFYY158 pKa = 10.35 LGTAAAEE165 pKa = 4.14 DD166 pKa = 4.15 APSYY170 pKa = 9.79 LASVACEE177 pKa = 3.84 INNPVPVAVIGFPDD191 pKa = 3.69 ARR193 pKa = 11.84 HH194 pKa = 5.72 VINVIVDD201 pKa = 3.52 DD202 pKa = 4.36 VVAVGDD208 pKa = 3.96 NLAEE212 pKa = 4.17 MVSIFPNPTSSVLNINLPSNIEE234 pKa = 4.11 VLSSALVDD242 pKa = 3.36 VLGRR246 pKa = 11.84 TTGVVYY252 pKa = 10.95 SNGQMDD258 pKa = 4.08 VSGLSQGVYY267 pKa = 9.83 FLKK270 pKa = 11.07 LEE272 pKa = 4.26 TTQGSLTQRR281 pKa = 11.84 IVKK284 pKa = 9.23 QQ285 pKa = 3.32
Molecular weight: 29.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.681
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.986
Patrickios 1.074
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A410G096|A0A410G096_9FLAO DUF3095 domain-containing protein OS=Aequorivita sp. H23M31 OX=2494375 GN=EI546_02620 PE=4 SV=1
MM1 pKa = 7.48 LLKK4 pKa = 10.16 HH5 pKa = 6.8 DD6 pKa = 4.58 RR7 pKa = 11.84 CTKK10 pKa = 8.57 NNKK13 pKa = 9.27 NFVHH17 pKa = 6.06 YY18 pKa = 9.5 FALSRR23 pKa = 11.84 FVVSFCRR30 pKa = 11.84 DD31 pKa = 3.08 SLQSRR36 pKa = 11.84 FVVLSSRR43 pKa = 11.84 GAFPSCYY50 pKa = 9.87 LPIVMPFNNQSPHH63 pKa = 5.21 RR64 pKa = 4.27
Molecular weight: 7.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.545
IPC_protein 10.058
Toseland 10.306
ProMoST 10.365
Dawson 10.467
Bjellqvist 10.204
Wikipedia 10.657
Rodwell 10.745
Grimsley 10.54
Solomon 10.54
Lehninger 10.526
Nozaki 10.394
DTASelect 10.175
Thurlkill 10.35
EMBOSS 10.701
Sillero 10.409
Patrickios 10.599
IPC_peptide 10.54
IPC2_peptide 9.545
IPC2.peptide.svr19 8.503
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3051
0
3051
1022300
20
3897
335.1
37.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.254 ± 0.043
0.726 ± 0.017
5.651 ± 0.04
6.926 ± 0.045
5.267 ± 0.036
6.561 ± 0.05
1.728 ± 0.02
7.885 ± 0.046
7.478 ± 0.072
9.29 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.324 ± 0.022
5.874 ± 0.044
3.623 ± 0.03
3.33 ± 0.024
3.82 ± 0.036
6.524 ± 0.038
5.54 ± 0.067
6.104 ± 0.033
1.039 ± 0.015
4.056 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here