Amino acid dipepetide frequency for Aequorivita sp. H23M31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.132AlaAla: 4.132 ± 0.094
0.515AlaCys: 0.515 ± 0.027
3.415AlaAsp: 3.415 ± 0.069
4.306AlaGlu: 4.306 ± 0.067
3.148AlaPhe: 3.148 ± 0.057
4.339AlaGly: 4.339 ± 0.078
1.069AlaHis: 1.069 ± 0.033
5.241AlaIle: 5.241 ± 0.08
4.205AlaLys: 4.205 ± 0.083
6.095AlaLeu: 6.095 ± 0.092
1.569AlaMet: 1.569 ± 0.045
3.248AlaAsn: 3.248 ± 0.086
1.959AlaPro: 1.959 ± 0.045
2.452AlaGln: 2.452 ± 0.056
2.235AlaArg: 2.235 ± 0.046
4.058AlaSer: 4.058 ± 0.078
3.622AlaThr: 3.622 ± 0.078
4.068AlaVal: 4.068 ± 0.062
0.564AlaTrp: 0.564 ± 0.024
2.303AlaTyr: 2.303 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.451CysAla: 0.451 ± 0.028
0.08CysCys: 0.08 ± 0.01
0.462CysAsp: 0.462 ± 0.028
0.514CysGlu: 0.514 ± 0.029
0.355CysPhe: 0.355 ± 0.021
0.679CysGly: 0.679 ± 0.033
0.147CysHis: 0.147 ± 0.013
0.539CysIle: 0.539 ± 0.021
0.425CysLys: 0.425 ± 0.022
0.57CysLeu: 0.57 ± 0.026
0.13CysMet: 0.13 ± 0.012
0.396CysAsn: 0.396 ± 0.024
0.332CysPro: 0.332 ± 0.021
0.206CysGln: 0.206 ± 0.014
0.244CysArg: 0.244 ± 0.014
0.539CysSer: 0.539 ± 0.029
0.418CysThr: 0.418 ± 0.024
0.411CysVal: 0.411 ± 0.022
0.067CysTrp: 0.067 ± 0.01
0.301CysTyr: 0.301 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.532AspAla: 3.532 ± 0.077
0.48AspCys: 0.48 ± 0.034
2.853AspAsp: 2.853 ± 0.066
3.864AspGlu: 3.864 ± 0.067
3.713AspPhe: 3.713 ± 0.071
3.986AspGly: 3.986 ± 0.102
0.917AspHis: 0.917 ± 0.032
4.362AspIle: 4.362 ± 0.064
3.966AspLys: 3.966 ± 0.073
5.423AspLeu: 5.423 ± 0.087
1.196AspMet: 1.196 ± 0.039
3.045AspAsn: 3.045 ± 0.078
2.091AspPro: 2.091 ± 0.052
1.48AspGln: 1.48 ± 0.037
2.226AspArg: 2.226 ± 0.045
3.613AspSer: 3.613 ± 0.066
2.665AspThr: 2.665 ± 0.051
3.477AspVal: 3.477 ± 0.064
0.738AspTrp: 0.738 ± 0.029
2.881AspTyr: 2.881 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
4.344GluAla: 4.344 ± 0.075
0.383GluCys: 0.383 ± 0.027
3.854GluAsp: 3.854 ± 0.063
5.292GluGlu: 5.292 ± 0.093
3.184GluPhe: 3.184 ± 0.056
3.879GluGly: 3.879 ± 0.065
1.089GluHis: 1.089 ± 0.035
6.056GluIle: 6.056 ± 0.084
6.255GluLys: 6.255 ± 0.1
6.045GluLeu: 6.045 ± 0.092
2.013GluMet: 2.013 ± 0.045
5.068GluAsn: 5.068 ± 0.075
1.758GluPro: 1.758 ± 0.047
2.031GluGln: 2.031 ± 0.047
2.93GluArg: 2.93 ± 0.063
3.524GluSer: 3.524 ± 0.055
3.794GluThr: 3.794 ± 0.066
4.395GluVal: 4.395 ± 0.07
0.673GluTrp: 0.673 ± 0.029
2.696GluTyr: 2.696 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.024PheAla: 3.024 ± 0.062
0.363PheCys: 0.363 ± 0.017
3.38PheAsp: 3.38 ± 0.065
3.53PheGlu: 3.53 ± 0.057
2.754PhePhe: 2.754 ± 0.057
3.706PheGly: 3.706 ± 0.074
0.927PheHis: 0.927 ± 0.031
3.889PheIle: 3.889 ± 0.082
3.511PheLys: 3.511 ± 0.064
4.971PheLeu: 4.971 ± 0.09
1.022PheMet: 1.022 ± 0.035
2.964PheAsn: 2.964 ± 0.06
2.025PhePro: 2.025 ± 0.04
1.768PheGln: 1.768 ± 0.041
1.94PheArg: 1.94 ± 0.042
4.074PheSer: 4.074 ± 0.073
2.969PheThr: 2.969 ± 0.06
3.089PheVal: 3.089 ± 0.056
0.554PheTrp: 0.554 ± 0.021
2.211PheTyr: 2.211 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
4.005GlyAla: 4.005 ± 0.076
0.572GlyCys: 0.572 ± 0.03
3.472GlyAsp: 3.472 ± 0.074
3.952GlyGlu: 3.952 ± 0.073
3.652GlyPhe: 3.652 ± 0.066
4.74GlyGly: 4.74 ± 0.106
1.092GlyHis: 1.092 ± 0.038
5.684GlyIle: 5.684 ± 0.095
5.084GlyLys: 5.084 ± 0.074
5.518GlyLeu: 5.518 ± 0.082
1.845GlyMet: 1.845 ± 0.049
4.094GlyAsn: 4.094 ± 0.094
1.568GlyPro: 1.568 ± 0.049
1.926GlyGln: 1.926 ± 0.049
2.419GlyArg: 2.419 ± 0.047
4.222GlySer: 4.222 ± 0.078
4.141GlyThr: 4.141 ± 0.125
4.078GlyVal: 4.078 ± 0.07
0.781GlyTrp: 0.781 ± 0.032
2.765GlyTyr: 2.765 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
0.881HisAla: 0.881 ± 0.031
0.178HisCys: 0.178 ± 0.013
0.79HisAsp: 0.79 ± 0.027
0.863HisGlu: 0.863 ± 0.029
1.223HisPhe: 1.223 ± 0.036
1.025HisGly: 1.025 ± 0.029
0.434HisHis: 0.434 ± 0.022
1.39HisIle: 1.39 ± 0.038
1.21HisLys: 1.21 ± 0.035
1.824HisLeu: 1.824 ± 0.043
0.273HisMet: 0.273 ± 0.017
0.996HisAsn: 0.996 ± 0.031
0.894HisPro: 0.894 ± 0.038
0.673HisGln: 0.673 ± 0.026
0.706HisArg: 0.706 ± 0.03
1.141HisSer: 1.141 ± 0.039
0.923HisThr: 0.923 ± 0.033
0.792HisVal: 0.792 ± 0.03
0.225HisTrp: 0.225 ± 0.015
0.835HisTyr: 0.835 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.499IleAla: 5.499 ± 0.086
0.65IleCys: 0.65 ± 0.029
4.788IleAsp: 4.788 ± 0.079
5.296IleGlu: 5.296 ± 0.078
3.921IlePhe: 3.921 ± 0.077
4.98IleGly: 4.98 ± 0.07
1.346IleHis: 1.346 ± 0.036
5.906IleIle: 5.906 ± 0.097
5.4IleLys: 5.4 ± 0.078
7.438IleLeu: 7.438 ± 0.11
1.491IleMet: 1.491 ± 0.033
4.193IleAsn: 4.193 ± 0.069
3.418IlePro: 3.418 ± 0.064
2.56IleGln: 2.56 ± 0.056
2.891IleArg: 2.891 ± 0.06
6.021IleSer: 6.021 ± 0.077
4.63IleThr: 4.63 ± 0.079
4.784IleVal: 4.784 ± 0.083
0.713IleTrp: 0.713 ± 0.027
2.929IleTyr: 2.929 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.283LysAla: 4.283 ± 0.079
0.337LysCys: 0.337 ± 0.019
4.447LysAsp: 4.447 ± 0.077
6.054LysGlu: 6.054 ± 0.109
3.071LysPhe: 3.071 ± 0.058
4.315LysGly: 4.315 ± 0.066
1.209LysHis: 1.209 ± 0.038
6.065LysIle: 6.065 ± 0.092
6.613LysLys: 6.613 ± 0.113
6.39LysLeu: 6.39 ± 0.09
2.202LysMet: 2.202 ± 0.05
5.161LysAsn: 5.161 ± 0.084
2.418LysPro: 2.418 ± 0.056
2.423LysGln: 2.423 ± 0.053
3.031LysArg: 3.031 ± 0.056
4.384LysSer: 4.384 ± 0.08
4.341LysThr: 4.341 ± 0.079
4.319LysVal: 4.319 ± 0.07
0.738LysTrp: 0.738 ± 0.029
2.984LysTyr: 2.984 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
5.892LeuAla: 5.892 ± 0.093
0.644LeuCys: 0.644 ± 0.027
5.308LeuAsp: 5.308 ± 0.081
6.52LeuGlu: 6.52 ± 0.093
4.922LeuPhe: 4.922 ± 0.078
5.833LeuGly: 5.833 ± 0.078
1.6LeuHis: 1.6 ± 0.046
6.88LeuIle: 6.88 ± 0.101
7.707LeuLys: 7.707 ± 0.11
8.865LeuLeu: 8.865 ± 0.139
2.177LeuMet: 2.177 ± 0.042
5.198LeuAsn: 5.198 ± 0.081
3.607LeuPro: 3.607 ± 0.065
3.312LeuGln: 3.312 ± 0.059
3.722LeuArg: 3.722 ± 0.065
6.533LeuSer: 6.533 ± 0.087
4.843LeuThr: 4.843 ± 0.079
5.281LeuVal: 5.281 ± 0.08
0.842LeuTrp: 0.842 ± 0.036
3.216LeuTyr: 3.216 ± 0.062
0.0LeuXaa: 0.0 ± 0.0
Met
1.857MetAla: 1.857 ± 0.045
0.147MetCys: 0.147 ± 0.012
1.521MetAsp: 1.521 ± 0.036
1.776MetGlu: 1.776 ± 0.042
0.877MetPhe: 0.877 ± 0.039
1.725MetGly: 1.725 ± 0.046
0.368MetHis: 0.368 ± 0.02
1.552MetIle: 1.552 ± 0.041
2.196MetLys: 2.196 ± 0.05
1.986MetLeu: 1.986 ± 0.04
0.592MetMet: 0.592 ± 0.028
1.293MetAsn: 1.293 ± 0.031
0.813MetPro: 0.813 ± 0.028
0.832MetGln: 0.832 ± 0.031
0.947MetArg: 0.947 ± 0.034
1.314MetSer: 1.314 ± 0.037
1.089MetThr: 1.089 ± 0.031
1.515MetVal: 1.515 ± 0.044
0.165MetTrp: 0.165 ± 0.012
0.679MetTyr: 0.679 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.565AsnAla: 3.565 ± 0.068
0.482AsnCys: 0.482 ± 0.026
2.987AsnAsp: 2.987 ± 0.052
3.613AsnGlu: 3.613 ± 0.065
3.148AsnPhe: 3.148 ± 0.063
4.064AsnGly: 4.064 ± 0.104
0.991AsnHis: 0.991 ± 0.033
4.579AsnIle: 4.579 ± 0.071
3.819AsnLys: 3.819 ± 0.075
5.668AsnLeu: 5.668 ± 0.086
1.232AsnMet: 1.232 ± 0.038
3.359AsnAsn: 3.359 ± 0.076
3.069AsnPro: 3.069 ± 0.068
2.029AsnGln: 2.029 ± 0.048
2.339AsnArg: 2.339 ± 0.054
3.802AsnSer: 3.802 ± 0.068
3.226AsnThr: 3.226 ± 0.073
3.388AsnVal: 3.388 ± 0.06
0.727AsnTrp: 0.727 ± 0.029
2.653AsnTyr: 2.653 ± 0.056
0.0AsnXaa: 0.0 ± 0.0
Pro
2.117ProAla: 2.117 ± 0.053
0.232ProCys: 0.232 ± 0.016
2.252ProAsp: 2.252 ± 0.058
3.086ProGlu: 3.086 ± 0.068
2.061ProPhe: 2.061 ± 0.045
2.191ProGly: 2.191 ± 0.058
0.699ProHis: 0.699 ± 0.024
2.816ProIle: 2.816 ± 0.056
2.637ProLys: 2.637 ± 0.055
3.105ProLeu: 3.105 ± 0.066
0.782ProMet: 0.782 ± 0.028
2.262ProAsn: 2.262 ± 0.047
1.042ProPro: 1.042 ± 0.034
1.3ProGln: 1.3 ± 0.043
1.096ProArg: 1.096 ± 0.032
2.351ProSer: 2.351 ± 0.052
1.986ProThr: 1.986 ± 0.052
2.37ProVal: 2.37 ± 0.059
0.336ProTrp: 0.336 ± 0.02
1.506ProTyr: 1.506 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
2.057GlnAla: 2.057 ± 0.047
0.167GlnCys: 0.167 ± 0.012
1.687GlnAsp: 1.687 ± 0.04
2.298GlnGlu: 2.298 ± 0.048
1.614GlnPhe: 1.614 ± 0.044
1.88GlnGly: 1.88 ± 0.05
0.545GlnHis: 0.545 ± 0.024
2.757GlnIle: 2.757 ± 0.052
2.932GlnLys: 2.932 ± 0.059
3.327GlnLeu: 3.327 ± 0.06
0.904GlnMet: 0.904 ± 0.033
2.194GlnAsn: 2.194 ± 0.053
1.114GlnPro: 1.114 ± 0.033
1.352GlnGln: 1.352 ± 0.045
1.361GlnArg: 1.361 ± 0.034
1.831GlnSer: 1.831 ± 0.048
1.759GlnThr: 1.759 ± 0.04
1.842GlnVal: 1.842 ± 0.041
0.36GlnTrp: 0.36 ± 0.019
1.321GlnTyr: 1.321 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.294ArgAla: 2.294 ± 0.046
0.193ArgCys: 0.193 ± 0.013
2.155ArgAsp: 2.155 ± 0.046
2.793ArgGlu: 2.793 ± 0.057
2.04ArgPhe: 2.04 ± 0.049
2.227ArgGly: 2.227 ± 0.048
0.659ArgHis: 0.659 ± 0.026
3.176ArgIle: 3.176 ± 0.056
3.11ArgLys: 3.11 ± 0.068
3.498ArgLeu: 3.498 ± 0.059
1.052ArgMet: 1.052 ± 0.033
2.486ArgAsn: 2.486 ± 0.058
1.245ArgPro: 1.245 ± 0.036
1.265ArgGln: 1.265 ± 0.038
1.571ArgArg: 1.571 ± 0.046
2.151ArgSer: 2.151 ± 0.051
2.037ArgThr: 2.037 ± 0.047
2.249ArgVal: 2.249 ± 0.055
0.379ArgTrp: 0.379 ± 0.019
1.618ArgTyr: 1.618 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
3.907SerAla: 3.907 ± 0.078
0.6SerCys: 0.6 ± 0.031
3.456SerAsp: 3.456 ± 0.064
4.813SerGlu: 4.813 ± 0.081
3.708SerPhe: 3.708 ± 0.071
4.747SerGly: 4.747 ± 0.093
1.153SerHis: 1.153 ± 0.035
5.062SerIle: 5.062 ± 0.08
4.585SerLys: 4.585 ± 0.086
6.191SerLeu: 6.191 ± 0.082
1.252SerMet: 1.252 ± 0.033
3.509SerAsn: 3.509 ± 0.065
2.373SerPro: 2.373 ± 0.046
2.289SerGln: 2.289 ± 0.047
2.346SerArg: 2.346 ± 0.055
4.359SerSer: 4.359 ± 0.09
3.469SerThr: 3.469 ± 0.068
4.007SerVal: 4.007 ± 0.072
0.739SerTrp: 0.739 ± 0.029
2.671SerTyr: 2.671 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
3.728ThrAla: 3.728 ± 0.069
0.347ThrCys: 0.347 ± 0.023
3.165ThrAsp: 3.165 ± 0.064
3.502ThrGlu: 3.502 ± 0.063
2.83ThrPhe: 2.83 ± 0.057
4.047ThrGly: 4.047 ± 0.081
1.015ThrHis: 1.015 ± 0.032
4.558ThrIle: 4.558 ± 0.097
3.339ThrLys: 3.339 ± 0.056
5.351ThrLeu: 5.351 ± 0.072
1.005ThrMet: 1.005 ± 0.039
2.885ThrAsn: 2.885 ± 0.07
2.359ThrPro: 2.359 ± 0.056
1.695ThrGln: 1.695 ± 0.045
1.742ThrArg: 1.742 ± 0.045
3.697ThrSer: 3.697 ± 0.076
3.312ThrThr: 3.312 ± 0.103
3.935ThrVal: 3.935 ± 0.115
0.575ThrTrp: 0.575 ± 0.027
2.31ThrTyr: 2.31 ± 0.071
0.0ThrXaa: 0.0 ± 0.0
Val
4.027ValAla: 4.027 ± 0.071
0.486ValCys: 0.486 ± 0.023
3.575ValAsp: 3.575 ± 0.065
4.132ValGlu: 4.132 ± 0.069
3.304ValPhe: 3.304 ± 0.065
3.903ValGly: 3.903 ± 0.067
1.026ValHis: 1.026 ± 0.032
4.689ValIle: 4.689 ± 0.075
4.138ValLys: 4.138 ± 0.069
5.832ValLeu: 5.832 ± 0.085
1.437ValMet: 1.437 ± 0.039
3.211ValAsn: 3.211 ± 0.059
2.338ValPro: 2.338 ± 0.048
1.903ValGln: 1.903 ± 0.049
2.244ValArg: 2.244 ± 0.05
4.321ValSer: 4.321 ± 0.07
3.345ValThr: 3.345 ± 0.115
4.192ValVal: 4.192 ± 0.084
0.578ValTrp: 0.578 ± 0.027
2.361ValTyr: 2.361 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.64TrpAla: 0.64 ± 0.026
0.074TrpCys: 0.074 ± 0.008
0.605TrpAsp: 0.605 ± 0.026
0.709TrpGlu: 0.709 ± 0.03
0.576TrpPhe: 0.576 ± 0.027
0.662TrpGly: 0.662 ± 0.026
0.208TrpHis: 0.208 ± 0.013
0.821TrpIle: 0.821 ± 0.027
0.769TrpLys: 0.769 ± 0.027
0.943TrpLeu: 0.943 ± 0.029
0.315TrpMet: 0.315 ± 0.018
0.723TrpAsn: 0.723 ± 0.031
0.259TrpPro: 0.259 ± 0.016
0.388TrpGln: 0.388 ± 0.019
0.448TrpArg: 0.448 ± 0.019
0.552TrpSer: 0.552 ± 0.025
0.547TrpThr: 0.547 ± 0.027
0.617TrpVal: 0.617 ± 0.029
0.144TrpTrp: 0.144 ± 0.013
0.385TrpTyr: 0.385 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.311TyrAla: 2.311 ± 0.045
0.334TyrCys: 0.334 ± 0.017
2.336TyrAsp: 2.336 ± 0.054
2.352TyrGlu: 2.352 ± 0.048
2.57TyrPhe: 2.57 ± 0.057
2.678TyrGly: 2.678 ± 0.046
0.783TyrHis: 0.783 ± 0.031
2.83TyrIle: 2.83 ± 0.049
2.888TyrLys: 2.888 ± 0.057
3.862TyrLeu: 3.862 ± 0.076
0.755TyrMet: 0.755 ± 0.024
2.455TyrAsn: 2.455 ± 0.059
1.545TyrPro: 1.545 ± 0.041
1.416TyrGln: 1.416 ± 0.042
1.759TyrArg: 1.759 ± 0.048
2.749TyrSer: 2.749 ± 0.059
2.283TyrThr: 2.283 ± 0.084
2.232TyrVal: 2.232 ± 0.047
0.49TyrTrp: 0.49 ± 0.02
1.932TyrTyr: 1.932 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3051 proteins (1022300 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski