Psychrobacillus glaciei

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Psychrobacillus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6SP85|A0A5J6SP85_9BACI Polysaccharide biosynthesis protein OS=Psychrobacillus glaciei OX=2283160 GN=PB01_13375 PE=4 SV=1
MM1 pKa = 7.72ALTLQAYY8 pKa = 5.53TTPTNTVVTADD19 pKa = 3.52TAAPPGTLPTPAVPTTPSILSPMIDD44 pKa = 3.8GSDD47 pKa = 3.31AYY49 pKa = 9.58IWSPNTTSNQTVKK62 pKa = 10.9FEE64 pKa = 4.26TTFDD68 pKa = 3.25IGTLPILSLDD78 pKa = 4.02LPISVYY84 pKa = 10.0FAYY87 pKa = 10.21AANEE91 pKa = 4.12IASVTASLEE100 pKa = 3.99IVNLLGIVVATIPLFTGSNSGSFQDD125 pKa = 3.52VAIASGDD132 pKa = 3.69ALVAAGLLGNTGRR145 pKa = 11.84IVVDD149 pKa = 3.39TTVTSPSVIGYY160 pKa = 6.94STNPGTYY167 pKa = 9.53LGEE170 pKa = 3.79ITVRR174 pKa = 11.84SLSIVV179 pKa = 3.21

Molecular weight: 18.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6STD6|A0A5J6STD6_9BACI ABC transporter ATP-binding protein OS=Psychrobacillus glaciei OX=2283160 GN=PB01_15905 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.55RR3 pKa = 11.84TFQPNSRR10 pKa = 11.84KK11 pKa = 9.5RR12 pKa = 11.84SKK14 pKa = 10.67NHH16 pKa = 4.97GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.52NGRR28 pKa = 11.84RR29 pKa = 11.84IIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3932

0

3932

1128006

19

3345

286.9

32.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.676 ± 0.045

0.667 ± 0.01

4.937 ± 0.034

7.466 ± 0.048

4.719 ± 0.034

6.526 ± 0.047

2.004 ± 0.021

8.391 ± 0.043

7.146 ± 0.04

9.784 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.019

4.778 ± 0.033

3.395 ± 0.021

3.599 ± 0.026

3.745 ± 0.03

6.243 ± 0.032

5.618 ± 0.036

6.994 ± 0.038

1.002 ± 0.015

3.538 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski