Amino acid dipepetide frequency for Psychrobacillus glaciei

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.009AlaAla: 5.009 ± 0.092
0.546AlaCys: 0.546 ± 0.02
3.169AlaAsp: 3.169 ± 0.058
4.215AlaGlu: 4.215 ± 0.074
3.353AlaPhe: 3.353 ± 0.061
4.645AlaGly: 4.645 ± 0.077
1.236AlaHis: 1.236 ± 0.033
6.294AlaIle: 6.294 ± 0.098
4.714AlaLys: 4.714 ± 0.075
6.708AlaLeu: 6.708 ± 0.088
1.93AlaMet: 1.93 ± 0.049
2.99AlaAsn: 2.99 ± 0.055
1.875AlaPro: 1.875 ± 0.049
2.185AlaGln: 2.185 ± 0.051
2.387AlaArg: 2.387 ± 0.05
4.126AlaSer: 4.126 ± 0.059
3.779AlaThr: 3.779 ± 0.084
4.747AlaVal: 4.747 ± 0.071
0.557AlaTrp: 0.557 ± 0.021
2.295AlaTyr: 2.295 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.379CysAla: 0.379 ± 0.018
0.064CysCys: 0.064 ± 0.007
0.347CysAsp: 0.347 ± 0.018
0.426CysGlu: 0.426 ± 0.023
0.319CysPhe: 0.319 ± 0.019
0.591CysGly: 0.591 ± 0.026
0.191CysHis: 0.191 ± 0.014
0.556CysIle: 0.556 ± 0.024
0.376CysLys: 0.376 ± 0.018
0.607CysLeu: 0.607 ± 0.025
0.168CysMet: 0.168 ± 0.013
0.289CysAsn: 0.289 ± 0.016
0.299CysPro: 0.299 ± 0.015
0.19CysGln: 0.19 ± 0.012
0.274CysArg: 0.274 ± 0.016
0.497CysSer: 0.497 ± 0.025
0.387CysThr: 0.387 ± 0.02
0.395CysVal: 0.395 ± 0.018
0.085CysTrp: 0.085 ± 0.009
0.231CysTyr: 0.231 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.401AspAla: 3.401 ± 0.067
0.318AspCys: 0.318 ± 0.017
2.28AspAsp: 2.28 ± 0.051
4.277AspGlu: 4.277 ± 0.076
2.578AspPhe: 2.578 ± 0.049
3.239AspGly: 3.239 ± 0.055
0.971AspHis: 0.971 ± 0.03
4.229AspIle: 4.229 ± 0.061
3.129AspLys: 3.129 ± 0.059
4.887AspLeu: 4.887 ± 0.075
1.284AspMet: 1.284 ± 0.037
2.01AspAsn: 2.01 ± 0.042
1.69AspPro: 1.69 ± 0.045
1.697AspGln: 1.697 ± 0.035
1.934AspArg: 1.934 ± 0.041
2.747AspSer: 2.747 ± 0.054
2.413AspThr: 2.413 ± 0.048
3.623AspVal: 3.623 ± 0.066
0.579AspTrp: 0.579 ± 0.024
2.087AspTyr: 2.087 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
5.036GluAla: 5.036 ± 0.091
0.345GluCys: 0.345 ± 0.019
3.653GluAsp: 3.653 ± 0.063
6.92GluGlu: 6.92 ± 0.101
2.695GluPhe: 2.695 ± 0.049
4.058GluGly: 4.058 ± 0.068
1.423GluHis: 1.423 ± 0.036
6.025GluIle: 6.025 ± 0.083
6.668GluLys: 6.668 ± 0.085
7.184GluLeu: 7.184 ± 0.107
2.365GluMet: 2.365 ± 0.049
3.955GluAsn: 3.955 ± 0.068
1.758GluPro: 1.758 ± 0.037
3.431GluGln: 3.431 ± 0.078
3.021GluArg: 3.021 ± 0.059
3.77GluSer: 3.77 ± 0.065
3.867GluThr: 3.867 ± 0.056
5.409GluVal: 5.409 ± 0.075
0.863GluTrp: 0.863 ± 0.029
2.217GluTyr: 2.217 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.002PheAla: 3.002 ± 0.051
0.322PheCys: 0.322 ± 0.016
2.456PheAsp: 2.456 ± 0.046
3.183PheGlu: 3.183 ± 0.052
2.469PhePhe: 2.469 ± 0.058
3.277PheGly: 3.277 ± 0.065
1.043PheHis: 1.043 ± 0.031
4.096PheIle: 4.096 ± 0.084
2.548PheLys: 2.548 ± 0.051
4.665PheLeu: 4.665 ± 0.084
1.194PheMet: 1.194 ± 0.033
2.1PheAsn: 2.1 ± 0.045
1.686PhePro: 1.686 ± 0.04
1.72PheGln: 1.72 ± 0.04
1.475PheArg: 1.475 ± 0.034
3.444PheSer: 3.444 ± 0.065
2.772PheThr: 2.772 ± 0.055
3.381PheVal: 3.381 ± 0.057
0.479PheTrp: 0.479 ± 0.022
1.879PheTyr: 1.879 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.531GlyAla: 4.531 ± 0.102
0.543GlyCys: 0.543 ± 0.025
3.054GlyAsp: 3.054 ± 0.059
4.246GlyGlu: 4.246 ± 0.072
3.317GlyPhe: 3.317 ± 0.057
4.449GlyGly: 4.449 ± 0.079
1.276GlyHis: 1.276 ± 0.041
6.019GlyIle: 6.019 ± 0.079
5.04GlyLys: 5.04 ± 0.065
6.018GlyLeu: 6.018 ± 0.089
1.979GlyMet: 1.979 ± 0.041
2.908GlyAsn: 2.908 ± 0.055
1.602GlyPro: 1.602 ± 0.039
2.043GlyGln: 2.043 ± 0.05
2.364GlyArg: 2.364 ± 0.051
3.89GlySer: 3.89 ± 0.061
3.923GlyThr: 3.923 ± 0.072
4.595GlyVal: 4.595 ± 0.068
0.699GlyTrp: 0.699 ± 0.025
2.762GlyTyr: 2.762 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.283HisAla: 1.283 ± 0.037
0.171HisCys: 0.171 ± 0.014
0.934HisAsp: 0.934 ± 0.027
1.322HisGlu: 1.322 ± 0.036
1.047HisPhe: 1.047 ± 0.034
1.316HisGly: 1.316 ± 0.035
0.579HisHis: 0.579 ± 0.024
1.584HisIle: 1.584 ± 0.04
1.025HisLys: 1.025 ± 0.029
2.173HisLeu: 2.173 ± 0.05
0.5HisMet: 0.5 ± 0.02
0.762HisAsn: 0.762 ± 0.027
1.014HisPro: 1.014 ± 0.03
0.805HisGln: 0.805 ± 0.028
0.762HisArg: 0.762 ± 0.024
1.264HisSer: 1.264 ± 0.041
1.107HisThr: 1.107 ± 0.03
1.364HisVal: 1.364 ± 0.031
0.193HisTrp: 0.193 ± 0.013
0.838HisTyr: 0.838 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.261IleAla: 6.261 ± 0.092
0.658IleCys: 0.658 ± 0.026
4.461IleAsp: 4.461 ± 0.075
6.215IleGlu: 6.215 ± 0.089
3.653IlePhe: 3.653 ± 0.068
6.144IleGly: 6.144 ± 0.088
1.744IleHis: 1.744 ± 0.041
6.904IleIle: 6.904 ± 0.102
4.788IleLys: 4.788 ± 0.068
7.782IleLeu: 7.782 ± 0.091
1.954IleMet: 1.954 ± 0.043
3.658IleAsn: 3.658 ± 0.07
3.5IlePro: 3.5 ± 0.055
3.26IleGln: 3.26 ± 0.053
3.228IleArg: 3.228 ± 0.064
5.637IleSer: 5.637 ± 0.075
4.619IleThr: 4.619 ± 0.071
6.138IleVal: 6.138 ± 0.089
0.727IleTrp: 0.727 ± 0.026
2.576IleTyr: 2.576 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.34LysAla: 4.34 ± 0.065
0.345LysCys: 0.345 ± 0.018
3.805LysAsp: 3.805 ± 0.067
7.033LysGlu: 7.033 ± 0.107
2.272LysPhe: 2.272 ± 0.043
4.34LysGly: 4.34 ± 0.064
1.316LysHis: 1.316 ± 0.031
5.105LysIle: 5.105 ± 0.076
6.146LysLys: 6.146 ± 0.088
6.29LysLeu: 6.29 ± 0.075
2.457LysMet: 2.457 ± 0.051
3.673LysAsn: 3.673 ± 0.065
2.049LysPro: 2.049 ± 0.04
2.962LysGln: 2.962 ± 0.049
3.15LysArg: 3.15 ± 0.056
3.943LysSer: 3.943 ± 0.064
3.86LysThr: 3.86 ± 0.069
5.024LysVal: 5.024 ± 0.084
0.932LysTrp: 0.932 ± 0.027
2.419LysTyr: 2.419 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
6.981LeuAla: 6.981 ± 0.094
0.666LeuCys: 0.666 ± 0.024
4.674LeuAsp: 4.674 ± 0.065
6.929LeuGlu: 6.929 ± 0.093
5.001LeuPhe: 5.001 ± 0.087
6.023LeuGly: 6.023 ± 0.086
1.955LeuHis: 1.955 ± 0.048
7.616LeuIle: 7.616 ± 0.096
6.886LeuLys: 6.886 ± 0.092
10.522LeuLeu: 10.522 ± 0.126
2.469LeuMet: 2.469 ± 0.047
4.428LeuAsn: 4.428 ± 0.076
3.783LeuPro: 3.783 ± 0.064
3.635LeuGln: 3.635 ± 0.065
3.457LeuArg: 3.457 ± 0.054
6.786LeuSer: 6.786 ± 0.075
5.548LeuThr: 5.548 ± 0.07
6.505LeuVal: 6.505 ± 0.093
0.818LeuTrp: 0.818 ± 0.031
3.156LeuTyr: 3.156 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
1.769MetAla: 1.769 ± 0.043
0.14MetCys: 0.14 ± 0.012
1.551MetAsp: 1.551 ± 0.041
2.152MetGlu: 2.152 ± 0.047
1.105MetPhe: 1.105 ± 0.036
1.637MetGly: 1.637 ± 0.044
0.528MetHis: 0.528 ± 0.025
2.148MetIle: 2.148 ± 0.047
2.572MetLys: 2.572 ± 0.042
2.512MetLeu: 2.512 ± 0.05
0.823MetMet: 0.823 ± 0.028
1.637MetAsn: 1.637 ± 0.039
0.94MetPro: 0.94 ± 0.027
0.991MetGln: 0.991 ± 0.033
1.084MetArg: 1.084 ± 0.031
1.736MetSer: 1.736 ± 0.037
1.74MetThr: 1.74 ± 0.039
1.585MetVal: 1.585 ± 0.04
0.202MetTrp: 0.202 ± 0.013
0.857MetTyr: 0.857 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.055AsnAla: 3.055 ± 0.056
0.315AsnCys: 0.315 ± 0.018
2.368AsnAsp: 2.368 ± 0.046
3.902AsnGlu: 3.902 ± 0.078
1.902AsnPhe: 1.902 ± 0.041
3.413AsnGly: 3.413 ± 0.065
0.984AsnHis: 0.984 ± 0.031
3.719AsnIle: 3.719 ± 0.061
3.436AsnLys: 3.436 ± 0.064
4.243AsnLeu: 4.243 ± 0.066
1.272AsnMet: 1.272 ± 0.034
2.487AsnAsn: 2.487 ± 0.056
2.106AsnPro: 2.106 ± 0.047
1.736AsnGln: 1.736 ± 0.046
2.091AsnArg: 2.091 ± 0.046
2.752AsnSer: 2.752 ± 0.055
2.461AsnThr: 2.461 ± 0.05
3.175AsnVal: 3.175 ± 0.051
0.558AsnTrp: 0.558 ± 0.026
1.809AsnTyr: 1.809 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
1.956ProAla: 1.956 ± 0.049
0.223ProCys: 0.223 ± 0.015
1.635ProAsp: 1.635 ± 0.036
2.455ProGlu: 2.455 ± 0.044
1.987ProPhe: 1.987 ± 0.04
2.026ProGly: 2.026 ± 0.053
0.745ProHis: 0.745 ± 0.028
3.079ProIle: 3.079 ± 0.059
2.246ProLys: 2.246 ± 0.041
3.369ProLeu: 3.369 ± 0.056
0.843ProMet: 0.843 ± 0.026
1.824ProAsn: 1.824 ± 0.038
0.87ProPro: 0.87 ± 0.03
0.98ProGln: 0.98 ± 0.027
0.955ProArg: 0.955 ± 0.029
2.303ProSer: 2.303 ± 0.048
2.207ProThr: 2.207 ± 0.046
2.569ProVal: 2.569 ± 0.057
0.332ProTrp: 0.332 ± 0.019
1.351ProTyr: 1.351 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.48GlnAla: 2.48 ± 0.052
0.192GlnCys: 0.192 ± 0.013
1.546GlnAsp: 1.546 ± 0.038
2.478GlnGlu: 2.478 ± 0.052
1.674GlnPhe: 1.674 ± 0.041
1.968GlnGly: 1.968 ± 0.044
0.734GlnHis: 0.734 ± 0.025
2.787GlnIle: 2.787 ± 0.056
2.67GlnLys: 2.67 ± 0.051
4.204GlnLeu: 4.204 ± 0.074
1.1GlnMet: 1.1 ± 0.033
1.757GlnAsn: 1.757 ± 0.039
1.137GlnPro: 1.137 ± 0.033
1.697GlnGln: 1.697 ± 0.047
1.294GlnArg: 1.294 ± 0.037
2.207GlnSer: 2.207 ± 0.045
2.015GlnThr: 2.015 ± 0.045
2.448GlnVal: 2.448 ± 0.048
0.35GlnTrp: 0.35 ± 0.017
1.253GlnTyr: 1.253 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.292ArgAla: 2.292 ± 0.052
0.248ArgCys: 0.248 ± 0.014
1.825ArgAsp: 1.825 ± 0.044
2.847ArgGlu: 2.847 ± 0.058
1.785ArgPhe: 1.785 ± 0.042
2.153ArgGly: 2.153 ± 0.043
0.75ArgHis: 0.75 ± 0.027
3.174ArgIle: 3.174 ± 0.053
3.046ArgLys: 3.046 ± 0.059
3.781ArgLeu: 3.781 ± 0.067
1.186ArgMet: 1.186 ± 0.032
1.898ArgAsn: 1.898 ± 0.042
1.2ArgPro: 1.2 ± 0.039
1.311ArgGln: 1.311 ± 0.038
1.565ArgArg: 1.565 ± 0.042
2.03ArgSer: 2.03 ± 0.044
2.004ArgThr: 2.004 ± 0.04
2.58ArgVal: 2.58 ± 0.048
0.39ArgTrp: 0.39 ± 0.018
1.384ArgTyr: 1.384 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
3.611SerAla: 3.611 ± 0.068
0.361SerCys: 0.361 ± 0.017
2.86SerAsp: 2.86 ± 0.056
3.989SerGlu: 3.989 ± 0.073
3.646SerPhe: 3.646 ± 0.054
4.148SerGly: 4.148 ± 0.071
1.161SerHis: 1.161 ± 0.033
5.938SerIle: 5.938 ± 0.085
4.436SerLys: 4.436 ± 0.063
6.266SerLeu: 6.266 ± 0.093
1.743SerMet: 1.743 ± 0.037
3.095SerAsn: 3.095 ± 0.06
2.174SerPro: 2.174 ± 0.038
1.818SerGln: 1.818 ± 0.038
2.226SerArg: 2.226 ± 0.045
4.206SerSer: 4.206 ± 0.068
3.543SerThr: 3.543 ± 0.059
4.32SerVal: 4.32 ± 0.061
0.582SerTrp: 0.582 ± 0.023
2.31SerTyr: 2.31 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
3.783ThrAla: 3.783 ± 0.062
0.293ThrCys: 0.293 ± 0.018
2.695ThrAsp: 2.695 ± 0.056
3.794ThrGlu: 3.794 ± 0.06
2.751ThrPhe: 2.751 ± 0.052
4.166ThrGly: 4.166 ± 0.15
1.107ThrHis: 1.107 ± 0.031
5.176ThrIle: 5.176 ± 0.07
3.836ThrLys: 3.836 ± 0.058
5.328ThrLeu: 5.328 ± 0.065
1.327ThrMet: 1.327 ± 0.037
2.813ThrAsn: 2.813 ± 0.052
2.309ThrPro: 2.309 ± 0.049
1.508ThrGln: 1.508 ± 0.031
1.746ThrArg: 1.746 ± 0.042
3.713ThrSer: 3.713 ± 0.066
3.319ThrThr: 3.319 ± 0.068
3.998ThrVal: 3.998 ± 0.068
0.524ThrTrp: 0.524 ± 0.025
1.992ThrTyr: 1.992 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
4.796ValAla: 4.796 ± 0.075
0.546ValCys: 0.546 ± 0.024
3.687ValAsp: 3.687 ± 0.062
5.069ValGlu: 5.069 ± 0.067
3.213ValPhe: 3.213 ± 0.057
4.664ValGly: 4.664 ± 0.072
1.381ValHis: 1.381 ± 0.041
5.816ValIle: 5.816 ± 0.086
4.756ValLys: 4.756 ± 0.076
6.831ValLeu: 6.831 ± 0.089
1.832ValMet: 1.832 ± 0.043
3.272ValAsn: 3.272 ± 0.059
2.44ValPro: 2.44 ± 0.049
2.432ValGln: 2.432 ± 0.047
2.519ValArg: 2.519 ± 0.06
4.582ValSer: 4.582 ± 0.064
4.117ValThr: 4.117 ± 0.076
5.189ValVal: 5.189 ± 0.077
0.626ValTrp: 0.626 ± 0.026
2.178ValTyr: 2.178 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.521TrpAla: 0.521 ± 0.025
0.066TrpCys: 0.066 ± 0.007
0.535TrpAsp: 0.535 ± 0.024
0.64TrpGlu: 0.64 ± 0.024
0.514TrpPhe: 0.514 ± 0.022
0.636TrpGly: 0.636 ± 0.023
0.202TrpHis: 0.202 ± 0.013
0.9TrpIle: 0.9 ± 0.032
0.801TrpLys: 0.801 ± 0.028
1.075TrpLeu: 1.075 ± 0.033
0.337TrpMet: 0.337 ± 0.019
0.592TrpAsn: 0.592 ± 0.021
0.237TrpPro: 0.237 ± 0.013
0.338TrpGln: 0.338 ± 0.017
0.377TrpArg: 0.377 ± 0.018
0.633TrpSer: 0.633 ± 0.028
0.556TrpThr: 0.556 ± 0.02
0.613TrpVal: 0.613 ± 0.028
0.116TrpTrp: 0.116 ± 0.01
0.325TrpTyr: 0.325 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.277TyrAla: 2.277 ± 0.045
0.31TyrCys: 0.31 ± 0.02
1.836TyrAsp: 1.836 ± 0.043
2.571TyrGlu: 2.571 ± 0.046
1.909TyrPhe: 1.909 ± 0.044
2.363TyrGly: 2.363 ± 0.052
0.719TyrHis: 0.719 ± 0.025
2.744TyrIle: 2.744 ± 0.054
2.341TyrLys: 2.341 ± 0.046
3.396TyrLeu: 3.396 ± 0.063
0.943TyrMet: 0.943 ± 0.032
1.637TyrAsn: 1.637 ± 0.043
1.285TyrPro: 1.285 ± 0.039
1.254TyrGln: 1.254 ± 0.034
1.539TyrArg: 1.539 ± 0.038
2.168TyrSer: 2.168 ± 0.045
1.941TyrThr: 1.941 ± 0.044
2.285TyrVal: 2.285 ± 0.047
0.402TyrTrp: 0.402 ± 0.018
1.459TyrTyr: 1.459 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3932 proteins (1128006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski