Rhodobacter phage RcapNL

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6WBN7|H6WBN7_9CAUD Phage integrase/recombinase OS=Rhodobacter phage RcapNL OX=1131316 GN=RcapNL_00034 PE=3 SV=1
MM1 pKa = 7.16TSFYY5 pKa = 10.64KK6 pKa = 9.34ITAYY10 pKa = 10.63NSQALYY16 pKa = 10.19FWGTDD21 pKa = 3.08ADD23 pKa = 3.4VDD25 pKa = 5.29RR26 pKa = 11.84YY27 pKa = 10.98VDD29 pKa = 3.06WLNRR33 pKa = 11.84DD34 pKa = 3.34RR35 pKa = 11.84EE36 pKa = 4.45INVYY40 pKa = 9.68AAEE43 pKa = 5.33AIPEE47 pKa = 4.26AEE49 pKa = 3.73WAQYY53 pKa = 9.77EE54 pKa = 4.22GRR56 pKa = 11.84DD57 pKa = 3.61DD58 pKa = 3.72VLSGEE63 pKa = 4.19EE64 pKa = 4.38CGWDD68 pKa = 3.12DD69 pKa = 5.12FMSAEE74 pKa = 4.0AA75 pKa = 4.65

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6WBR1|H6WBR1_9CAUD Uncharacterized protein OS=Rhodobacter phage RcapNL OX=1131316 GN=RcapNL_00058 PE=4 SV=1
MM1 pKa = 6.73SAKK4 pKa = 8.84WRR6 pKa = 11.84CRR8 pKa = 11.84LFWGNPHH15 pKa = 5.41TTPPHH20 pKa = 5.16GMPRR24 pKa = 11.84IALSVLCEE32 pKa = 4.02RR33 pKa = 11.84TSPIPNEE40 pKa = 3.92ILQMSGPGTEE50 pKa = 4.16YY51 pKa = 11.1APGSGWTVGWEE62 pKa = 4.36LIEE65 pKa = 3.95QRR67 pKa = 11.84PVRR70 pKa = 11.84RR71 pKa = 11.84WSAEE75 pKa = 3.2AKK77 pKa = 10.38GRR79 pKa = 11.84VRR81 pKa = 11.84QLNLRR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84IEE90 pKa = 3.7KK91 pKa = 10.25RR92 pKa = 11.84FPLFAEE98 pKa = 4.09MFIADD103 pKa = 3.87EE104 pKa = 4.14LARR107 pKa = 11.84RR108 pKa = 11.84PQYY111 pKa = 10.79FRR113 pKa = 11.84GEE115 pKa = 3.92AA116 pKa = 3.15

Molecular weight: 13.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12672

54

651

198.0

21.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.41 ± 0.538

1.065 ± 0.118

6.258 ± 0.228

5.54 ± 0.314

2.778 ± 0.153

9.312 ± 0.314

1.815 ± 0.182

4.593 ± 0.199

3.062 ± 0.261

8.112 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.169

2.304 ± 0.155

5.208 ± 0.219

3.212 ± 0.172

7.678 ± 0.314

4.845 ± 0.271

5.903 ± 0.361

7.039 ± 0.281

2.06 ± 0.182

1.949 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski