Amino acid dipepetide frequency for Rhodobacter phage RcapNL

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.307AlaAla: 24.307 ± 2.135
1.184AlaCys: 1.184 ± 0.32
8.602AlaAsp: 8.602 ± 0.889
9.944AlaGlu: 9.944 ± 1.0
3.315AlaPhe: 3.315 ± 0.586
12.469AlaGly: 12.469 ± 1.44
2.21AlaHis: 2.21 ± 0.45
6.235AlaIle: 6.235 ± 0.797
4.262AlaLys: 4.262 ± 0.712
12.943AlaLeu: 12.943 ± 1.295
5.051AlaMet: 5.051 ± 0.658
3.078AlaAsn: 3.078 ± 0.475
7.024AlaPro: 7.024 ± 1.288
5.13AlaGln: 5.13 ± 0.642
9.549AlaArg: 9.549 ± 0.78
7.419AlaSer: 7.419 ± 0.727
5.84AlaThr: 5.84 ± 0.662
10.575AlaVal: 10.575 ± 0.857
2.683AlaTrp: 2.683 ± 0.479
2.21AlaTyr: 2.21 ± 0.504
0.0AlaXaa: 0.0 ± 0.0
Cys
1.421CysAla: 1.421 ± 0.339
0.316CysCys: 0.316 ± 0.159
0.552CysAsp: 0.552 ± 0.26
0.552CysGlu: 0.552 ± 0.177
0.316CysPhe: 0.316 ± 0.132
1.263CysGly: 1.263 ± 0.396
0.079CysHis: 0.079 ± 0.08
0.474CysIle: 0.474 ± 0.165
0.474CysLys: 0.474 ± 0.16
0.947CysLeu: 0.947 ± 0.3
0.158CysMet: 0.158 ± 0.091
0.158CysAsn: 0.158 ± 0.118
0.947CysPro: 0.947 ± 0.257
0.237CysGln: 0.237 ± 0.145
0.552CysArg: 0.552 ± 0.206
0.237CysSer: 0.237 ± 0.137
0.631CysThr: 0.631 ± 0.234
0.947CysVal: 0.947 ± 0.288
0.079CysTrp: 0.079 ± 0.082
0.316CysTyr: 0.316 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
10.023AspAla: 10.023 ± 0.89
1.026AspCys: 1.026 ± 0.29
4.42AspAsp: 4.42 ± 0.577
3.315AspGlu: 3.315 ± 0.655
1.657AspPhe: 1.657 ± 0.338
7.497AspGly: 7.497 ± 0.89
1.263AspHis: 1.263 ± 0.275
1.973AspIle: 1.973 ± 0.457
1.105AspLys: 1.105 ± 0.367
6.077AspLeu: 6.077 ± 0.655
1.026AspMet: 1.026 ± 0.326
0.868AspAsn: 0.868 ± 0.216
3.63AspPro: 3.63 ± 0.626
2.052AspGln: 2.052 ± 0.488
4.341AspArg: 4.341 ± 0.593
2.368AspSer: 2.368 ± 0.406
2.683AspThr: 2.683 ± 0.414
4.025AspVal: 4.025 ± 0.578
1.894AspTrp: 1.894 ± 0.38
1.342AspTyr: 1.342 ± 0.316
0.0AspXaa: 0.0 ± 0.0
Glu
10.575GluAla: 10.575 ± 1.056
0.474GluCys: 0.474 ± 0.167
3.315GluAsp: 3.315 ± 0.47
1.263GluGlu: 1.263 ± 0.457
1.736GluPhe: 1.736 ± 0.402
3.472GluGly: 3.472 ± 0.403
0.947GluHis: 0.947 ± 0.323
3.867GluIle: 3.867 ± 0.637
0.868GluLys: 0.868 ± 0.293
5.603GluLeu: 5.603 ± 0.675
1.657GluMet: 1.657 ± 0.38
1.263GluAsn: 1.263 ± 0.392
1.578GluPro: 1.578 ± 0.388
1.894GluGln: 1.894 ± 0.348
5.446GluArg: 5.446 ± 0.812
1.815GluSer: 1.815 ± 0.323
2.841GluThr: 2.841 ± 0.475
4.577GluVal: 4.577 ± 0.658
1.105GluTrp: 1.105 ± 0.438
1.105GluTyr: 1.105 ± 0.249
0.0GluXaa: 0.0 ± 0.0
Phe
3.709PheAla: 3.709 ± 0.769
0.079PheCys: 0.079 ± 0.073
2.131PheAsp: 2.131 ± 0.419
2.604PheGlu: 2.604 ± 0.429
0.395PhePhe: 0.395 ± 0.187
2.92PheGly: 2.92 ± 0.482
0.552PheHis: 0.552 ± 0.194
0.395PheIle: 0.395 ± 0.206
0.947PheLys: 0.947 ± 0.254
2.131PheLeu: 2.131 ± 0.409
0.631PheMet: 0.631 ± 0.232
0.552PheAsn: 0.552 ± 0.222
1.184PhePro: 1.184 ± 0.242
0.71PheGln: 0.71 ± 0.206
1.894PheArg: 1.894 ± 0.317
1.736PheSer: 1.736 ± 0.396
1.421PheThr: 1.421 ± 0.416
2.447PheVal: 2.447 ± 0.375
0.947PheTrp: 0.947 ± 0.223
0.395PheTyr: 0.395 ± 0.175
0.0PheXaa: 0.0 ± 0.0
Gly
11.917GlyAla: 11.917 ± 0.881
1.026GlyCys: 1.026 ± 0.279
5.603GlyAsp: 5.603 ± 0.885
4.735GlyGlu: 4.735 ± 0.608
2.447GlyPhe: 2.447 ± 0.415
9.786GlyGly: 9.786 ± 1.178
1.894GlyHis: 1.894 ± 0.377
2.525GlyIle: 2.525 ± 0.43
3.709GlyLys: 3.709 ± 0.527
6.629GlyLeu: 6.629 ± 0.843
3.078GlyMet: 3.078 ± 0.375
2.841GlyAsn: 2.841 ± 0.425
4.893GlyPro: 4.893 ± 0.528
2.841GlyGln: 2.841 ± 0.352
7.024GlyArg: 7.024 ± 0.813
5.13GlySer: 5.13 ± 0.746
5.998GlyThr: 5.998 ± 0.927
6.156GlyVal: 6.156 ± 0.679
2.131GlyTrp: 2.131 ± 0.357
2.762GlyTyr: 2.762 ± 1.138
0.0GlyXaa: 0.0 ± 0.0
His
1.815HisAla: 1.815 ± 0.384
0.316HisCys: 0.316 ± 0.185
1.105HisAsp: 1.105 ± 0.243
0.789HisGlu: 0.789 ± 0.249
0.474HisPhe: 0.474 ± 0.168
2.052HisGly: 2.052 ± 0.47
0.552HisHis: 0.552 ± 0.225
0.395HisIle: 0.395 ± 0.203
0.868HisLys: 0.868 ± 0.294
1.578HisLeu: 1.578 ± 0.373
0.552HisMet: 0.552 ± 0.194
0.552HisAsn: 0.552 ± 0.196
1.184HisPro: 1.184 ± 0.321
0.474HisGln: 0.474 ± 0.182
1.105HisArg: 1.105 ± 0.322
0.868HisSer: 0.868 ± 0.307
0.947HisThr: 0.947 ± 0.275
1.342HisVal: 1.342 ± 0.327
0.71HisTrp: 0.71 ± 0.232
0.474HisTyr: 0.474 ± 0.235
0.0HisXaa: 0.0 ± 0.0
Ile
6.235IleAla: 6.235 ± 0.593
0.395IleCys: 0.395 ± 0.172
3.946IleAsp: 3.946 ± 0.719
4.104IleGlu: 4.104 ± 0.626
1.026IlePhe: 1.026 ± 0.248
4.656IleGly: 4.656 ± 0.557
0.552IleHis: 0.552 ± 0.211
1.894IleIle: 1.894 ± 0.369
1.657IleLys: 1.657 ± 0.38
2.368IleLeu: 2.368 ± 0.428
0.71IleMet: 0.71 ± 0.239
1.105IleAsn: 1.105 ± 0.263
2.289IlePro: 2.289 ± 0.366
0.947IleGln: 0.947 ± 0.255
4.656IleArg: 4.656 ± 0.618
1.973IleSer: 1.973 ± 0.312
3.63IleThr: 3.63 ± 0.496
2.052IleVal: 2.052 ± 0.42
1.342IleTrp: 1.342 ± 0.311
0.395IleTyr: 0.395 ± 0.206
0.0IleXaa: 0.0 ± 0.0
Lys
3.63LysAla: 3.63 ± 0.61
0.158LysCys: 0.158 ± 0.107
1.421LysAsp: 1.421 ± 0.356
1.184LysGlu: 1.184 ± 0.294
0.552LysPhe: 0.552 ± 0.174
2.289LysGly: 2.289 ± 0.439
0.868LysHis: 0.868 ± 0.276
1.815LysIle: 1.815 ± 0.422
1.421LysLys: 1.421 ± 0.304
2.447LysLeu: 2.447 ± 0.402
0.868LysMet: 0.868 ± 0.227
0.71LysAsn: 0.71 ± 0.202
2.447LysPro: 2.447 ± 0.459
1.184LysGln: 1.184 ± 0.384
2.368LysArg: 2.368 ± 0.43
1.421LysSer: 1.421 ± 0.362
2.762LysThr: 2.762 ± 0.494
2.131LysVal: 2.131 ± 0.453
0.552LysTrp: 0.552 ± 0.212
0.395LysTyr: 0.395 ± 0.179
0.0LysXaa: 0.0 ± 0.0
Leu
10.97LeuAla: 10.97 ± 1.013
1.578LeuCys: 1.578 ± 0.342
5.524LeuAsp: 5.524 ± 0.715
4.104LeuGlu: 4.104 ± 0.644
2.447LeuPhe: 2.447 ± 0.362
7.024LeuGly: 7.024 ± 0.704
1.263LeuHis: 1.263 ± 0.324
4.262LeuIle: 4.262 ± 0.608
2.289LeuLys: 2.289 ± 0.471
5.367LeuLeu: 5.367 ± 0.743
1.894LeuMet: 1.894 ± 0.451
1.736LeuAsn: 1.736 ± 0.458
4.972LeuPro: 4.972 ± 0.648
2.683LeuGln: 2.683 ± 0.438
7.182LeuArg: 7.182 ± 0.726
4.972LeuSer: 4.972 ± 0.571
5.682LeuThr: 5.682 ± 0.65
5.288LeuVal: 5.288 ± 0.587
0.71LeuTrp: 0.71 ± 0.231
1.184LeuTyr: 1.184 ± 0.343
0.0LeuXaa: 0.0 ± 0.0
Met
3.788MetAla: 3.788 ± 0.54
0.316MetCys: 0.316 ± 0.161
1.421MetAsp: 1.421 ± 0.293
1.342MetGlu: 1.342 ± 0.332
0.71MetPhe: 0.71 ± 0.212
2.131MetGly: 2.131 ± 0.456
0.631MetHis: 0.631 ± 0.167
1.657MetIle: 1.657 ± 0.343
0.789MetLys: 0.789 ± 0.293
2.131MetLeu: 2.131 ± 0.519
0.552MetMet: 0.552 ± 0.195
0.316MetAsn: 0.316 ± 0.154
1.499MetPro: 1.499 ± 0.321
1.026MetGln: 1.026 ± 0.345
2.92MetArg: 2.92 ± 0.47
2.131MetSer: 2.131 ± 0.466
2.841MetThr: 2.841 ± 0.52
1.815MetVal: 1.815 ± 0.366
0.158MetTrp: 0.158 ± 0.105
0.316MetTyr: 0.316 ± 0.144
0.0MetXaa: 0.0 ± 0.0
Asn
2.683AsnAla: 2.683 ± 0.438
0.237AsnCys: 0.237 ± 0.116
1.342AsnAsp: 1.342 ± 0.342
0.947AsnGlu: 0.947 ± 0.324
0.789AsnPhe: 0.789 ± 0.24
3.078AsnGly: 3.078 ± 0.513
0.552AsnHis: 0.552 ± 0.247
1.815AsnIle: 1.815 ± 0.341
0.474AsnLys: 0.474 ± 0.198
1.421AsnLeu: 1.421 ± 0.284
0.789AsnMet: 0.789 ± 0.197
0.474AsnAsn: 0.474 ± 0.182
0.947AsnPro: 0.947 ± 0.273
0.631AsnGln: 0.631 ± 0.273
1.894AsnArg: 1.894 ± 0.276
1.105AsnSer: 1.105 ± 0.273
1.499AsnThr: 1.499 ± 0.353
1.342AsnVal: 1.342 ± 0.328
0.631AsnTrp: 0.631 ± 0.221
0.395AsnTyr: 0.395 ± 0.153
0.0AsnXaa: 0.0 ± 0.0
Pro
6.629ProAla: 6.629 ± 1.121
0.474ProCys: 0.474 ± 0.195
3.709ProAsp: 3.709 ± 0.417
4.104ProGlu: 4.104 ± 0.517
1.184ProPhe: 1.184 ± 0.224
3.551ProGly: 3.551 ± 0.468
0.868ProHis: 0.868 ± 0.279
2.525ProIle: 2.525 ± 0.357
1.894ProLys: 1.894 ± 0.378
3.788ProLeu: 3.788 ± 0.628
1.578ProMet: 1.578 ± 0.469
1.105ProAsn: 1.105 ± 0.265
2.92ProPro: 2.92 ± 0.42
1.815ProGln: 1.815 ± 0.334
3.946ProArg: 3.946 ± 0.458
2.999ProSer: 2.999 ± 0.481
2.762ProThr: 2.762 ± 0.481
4.893ProVal: 4.893 ± 0.619
0.789ProTrp: 0.789 ± 0.235
0.552ProTyr: 0.552 ± 0.261
0.0ProXaa: 0.0 ± 0.0
Gln
5.603GlnAla: 5.603 ± 0.588
0.316GlnCys: 0.316 ± 0.13
1.736GlnAsp: 1.736 ± 0.301
1.342GlnGlu: 1.342 ± 0.412
1.263GlnPhe: 1.263 ± 0.359
2.052GlnGly: 2.052 ± 0.405
0.71GlnHis: 0.71 ± 0.252
2.368GlnIle: 2.368 ± 0.421
0.71GlnLys: 0.71 ± 0.212
2.683GlnLeu: 2.683 ± 0.418
1.105GlnMet: 1.105 ± 0.265
1.184GlnAsn: 1.184 ± 0.292
1.342GlnPro: 1.342 ± 0.253
1.105GlnGln: 1.105 ± 0.264
2.368GlnArg: 2.368 ± 0.47
1.578GlnSer: 1.578 ± 0.318
1.105GlnThr: 1.105 ± 0.361
1.973GlnVal: 1.973 ± 0.402
0.71GlnTrp: 0.71 ± 0.249
0.868GlnTyr: 0.868 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
11.996ArgAla: 11.996 ± 1.134
0.789ArgCys: 0.789 ± 0.249
4.735ArgAsp: 4.735 ± 0.564
4.893ArgGlu: 4.893 ± 0.541
2.841ArgPhe: 2.841 ± 0.343
5.524ArgGly: 5.524 ± 0.846
1.421ArgHis: 1.421 ± 0.304
4.025ArgIle: 4.025 ± 0.485
2.762ArgLys: 2.762 ± 0.543
6.55ArgLeu: 6.55 ± 0.639
3.472ArgMet: 3.472 ± 0.528
1.736ArgAsn: 1.736 ± 0.448
3.472ArgPro: 3.472 ± 0.413
2.052ArgGln: 2.052 ± 0.383
6.235ArgArg: 6.235 ± 0.818
2.604ArgSer: 2.604 ± 0.468
2.841ArgThr: 2.841 ± 0.39
5.13ArgVal: 5.13 ± 0.802
1.736ArgTrp: 1.736 ± 0.419
1.894ArgTyr: 1.894 ± 0.341
0.079ArgXaa: 0.079 ± 0.08
Ser
7.024SerAla: 7.024 ± 0.9
0.474SerCys: 0.474 ± 0.169
3.078SerAsp: 3.078 ± 0.521
2.052SerGlu: 2.052 ± 0.394
0.789SerPhe: 0.789 ± 0.264
5.524SerGly: 5.524 ± 0.764
1.026SerHis: 1.026 ± 0.356
2.525SerIle: 2.525 ± 0.483
1.499SerLys: 1.499 ± 0.36
3.709SerLeu: 3.709 ± 0.605
1.421SerMet: 1.421 ± 0.368
1.184SerAsn: 1.184 ± 0.274
2.447SerPro: 2.447 ± 0.503
2.289SerGln: 2.289 ± 0.369
2.368SerArg: 2.368 ± 0.526
1.815SerSer: 1.815 ± 0.395
3.078SerThr: 3.078 ± 0.537
3.63SerVal: 3.63 ± 0.484
1.105SerTrp: 1.105 ± 0.362
1.421SerTyr: 1.421 ± 0.505
0.0SerXaa: 0.0 ± 0.0
Thr
7.34ThrAla: 7.34 ± 1.015
0.395ThrCys: 0.395 ± 0.155
3.788ThrAsp: 3.788 ± 0.534
2.525ThrGlu: 2.525 ± 0.465
2.368ThrPhe: 2.368 ± 0.492
6.945ThrGly: 6.945 ± 0.799
1.105ThrHis: 1.105 ± 0.283
2.604ThrIle: 2.604 ± 0.491
1.894ThrLys: 1.894 ± 0.4
5.209ThrLeu: 5.209 ± 0.582
1.184ThrMet: 1.184 ± 0.261
1.657ThrAsn: 1.657 ± 0.351
4.183ThrPro: 4.183 ± 0.653
1.263ThrGln: 1.263 ± 0.282
3.394ThrArg: 3.394 ± 0.454
2.052ThrSer: 2.052 ± 0.364
4.025ThrThr: 4.025 ± 0.532
4.577ThrVal: 4.577 ± 0.79
1.263ThrTrp: 1.263 ± 0.332
1.263ThrTyr: 1.263 ± 0.324
0.0ThrXaa: 0.0 ± 0.0
Val
10.26ValAla: 10.26 ± 0.914
0.474ValCys: 0.474 ± 0.152
3.709ValAsp: 3.709 ± 0.465
3.63ValGlu: 3.63 ± 0.409
2.131ValPhe: 2.131 ± 0.395
5.761ValGly: 5.761 ± 0.623
0.631ValHis: 0.631 ± 0.22
3.315ValIle: 3.315 ± 0.43
2.447ValLys: 2.447 ± 0.558
5.288ValLeu: 5.288 ± 0.69
2.052ValMet: 2.052 ± 0.395
1.657ValAsn: 1.657 ± 0.352
3.472ValPro: 3.472 ± 0.48
2.447ValGln: 2.447 ± 0.364
5.84ValArg: 5.84 ± 0.543
4.025ValSer: 4.025 ± 0.66
5.524ValThr: 5.524 ± 0.68
4.972ValVal: 4.972 ± 0.803
1.263ValTrp: 1.263 ± 0.332
1.499ValTyr: 1.499 ± 0.346
0.0ValXaa: 0.0 ± 0.0
Trp
1.342TrpAla: 1.342 ± 0.358
0.395TrpCys: 0.395 ± 0.171
1.263TrpAsp: 1.263 ± 0.322
1.184TrpGlu: 1.184 ± 0.282
0.237TrpPhe: 0.237 ± 0.13
1.736TrpGly: 1.736 ± 0.401
0.631TrpHis: 0.631 ± 0.258
1.105TrpIle: 1.105 ± 0.261
0.237TrpLys: 0.237 ± 0.134
2.131TrpLeu: 2.131 ± 0.373
0.237TrpMet: 0.237 ± 0.159
0.552TrpAsn: 0.552 ± 0.186
1.026TrpPro: 1.026 ± 0.236
0.71TrpGln: 0.71 ± 0.215
2.21TrpArg: 2.21 ± 0.379
1.657TrpSer: 1.657 ± 0.337
2.052TrpThr: 2.052 ± 0.426
1.263TrpVal: 1.263 ± 0.293
0.316TrpTrp: 0.316 ± 0.136
0.316TrpTyr: 0.316 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.841TyrAla: 2.841 ± 0.476
0.237TyrCys: 0.237 ± 0.128
1.184TyrAsp: 1.184 ± 0.27
0.789TyrGlu: 0.789 ± 0.256
1.105TyrPhe: 1.105 ± 0.36
3.394TyrGly: 3.394 ± 1.28
0.395TyrHis: 0.395 ± 0.202
0.158TyrIle: 0.158 ± 0.122
0.316TyrLys: 0.316 ± 0.151
2.131TyrLeu: 2.131 ± 0.387
0.395TyrMet: 0.395 ± 0.165
0.316TyrAsn: 0.316 ± 0.196
0.631TyrPro: 0.631 ± 0.235
0.631TyrGln: 0.631 ± 0.198
1.499TyrArg: 1.499 ± 0.372
0.552TyrSer: 0.552 ± 0.226
0.789TyrThr: 0.789 ± 0.261
1.263TyrVal: 1.263 ± 0.314
0.474TyrTrp: 0.474 ± 0.187
0.395TyrTyr: 0.395 ± 0.172
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.079XaaThr: 0.079 ± 0.08
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (12672 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski