Tupaia chinensis (Chinese tree shrew)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Scandentia;

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20824 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8Y5B3|L8Y5B3_TUPCH DNA damage-binding protein 1 OS=Tupaia chinensis OX=246437 GN=TREES_T100002304 PE=3 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84DD3 pKa = 2.99SSDD6 pKa = 2.96ISRR9 pKa = 11.84ICYY12 pKa = 9.9NGLPSNVHH20 pKa = 5.01VCSGADD26 pKa = 2.9CGLGNEE32 pKa = 4.42NAVKK36 pKa = 10.18QAEE39 pKa = 4.36TLFQQICPNEE49 pKa = 4.49DD50 pKa = 3.38FCPPPPNPEE59 pKa = 4.92DD60 pKa = 3.23IVLDD64 pKa = 4.03GDD66 pKa = 4.14SLQPEE71 pKa = 4.36ASEE74 pKa = 4.32SSAIPEE80 pKa = 4.53ANSEE84 pKa = 4.13APKK87 pKa = 10.56EE88 pKa = 4.26STNLGSPEE96 pKa = 4.02EE97 pKa = 4.16SSEE100 pKa = 3.98

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L9L7X0|L9L7X0_TUPCH 60S ribosomal protein L31 OS=Tupaia chinensis OX=246437 GN=TREES_T100011561 PE=3 SV=1
MM1 pKa = 7.89PPPGVPGGNAGRR13 pKa = 11.84GGGAQAAPQRR23 pKa = 11.84PARR26 pKa = 11.84RR27 pKa = 11.84PGPPPAGRR35 pKa = 11.84PGAVTPPGGAAARR48 pKa = 11.84LALAQRR54 pKa = 11.84EE55 pKa = 4.23RR56 pKa = 11.84FSPVRR61 pKa = 11.84GGGAGRR67 pKa = 11.84LRR69 pKa = 11.84FISRR73 pKa = 11.84RR74 pKa = 11.84HH75 pKa = 4.74PQAPGRR81 pKa = 11.84RR82 pKa = 11.84NEE84 pKa = 3.93SPRR87 pKa = 11.84AQGHH91 pKa = 4.93VRR93 pKa = 11.84RR94 pKa = 11.84TWTLRR99 pKa = 11.84APVTT103 pKa = 3.68

Molecular weight: 10.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20824

0

20824

9847371

21

29519

472.9

52.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.171 ± 0.021

2.287 ± 0.014

4.715 ± 0.014

6.947 ± 0.028

3.62 ± 0.013

6.644 ± 0.025

2.593 ± 0.009

4.229 ± 0.018

5.601 ± 0.028

10.01 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.167 ± 0.009

3.366 ± 0.013

6.205 ± 0.027

4.625 ± 0.017

5.751 ± 0.017

8.137 ± 0.026

5.358 ± 0.02

6.303 ± 0.02

1.22 ± 0.007

2.607 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski