Lysobacter dokdonensis DS-58
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3154 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A2WN33|A0A0A2WN33_9GAMM tRNA dimethylallyltransferase OS=Lysobacter dokdonensis DS-58 OX=1300345 GN=miaA PE=3 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.11 NDD4 pKa = 3.27 RR5 pKa = 11.84 TRR7 pKa = 11.84 ASRR10 pKa = 11.84 KK11 pKa = 8.07 AARR14 pKa = 11.84 RR15 pKa = 11.84 YY16 pKa = 9.32 NVSPVARR23 pKa = 11.84 AVRR26 pKa = 11.84 SVLTLSTAALLFSGTGAVLAKK47 pKa = 10.53 SPVLAPLPVEE57 pKa = 4.17 RR58 pKa = 11.84 VGLSHH63 pKa = 7.0 EE64 pKa = 4.17 FAKK67 pKa = 10.99 VVDD70 pKa = 4.24 LTRR73 pKa = 11.84 VADD76 pKa = 3.43 EE77 pKa = 4.14 WTPKK81 pKa = 10.58 SVVDD85 pKa = 5.14 EE86 pKa = 4.49 EE87 pKa = 4.47 FQSLAAPIGASEE99 pKa = 4.26 ATMFAAGAQPEE110 pKa = 4.51 SLGVVDD116 pKa = 6.29 DD117 pKa = 4.0 PTILPGSFHH126 pKa = 7.37 PGPGGVSGGDD136 pKa = 3.28 DD137 pKa = 2.95 AVLVNIGTGGNWTMVDD153 pKa = 4.07 GYY155 pKa = 11.45 APAPVVSNTGFADD168 pKa = 4.14 GAFFGTADD176 pKa = 3.22 SVTVRR181 pKa = 11.84 NYY183 pKa = 9.86 WDD185 pKa = 3.69 GLSVTGYY192 pKa = 7.4 TWAAGIEE199 pKa = 4.28 VEE201 pKa = 5.21 SGDD204 pKa = 3.53 EE205 pKa = 4.01 GLIGSYY211 pKa = 10.74 DD212 pKa = 4.52 AITATATGVDD222 pKa = 4.01 GVAFGLYY229 pKa = 10.21 VYY231 pKa = 10.01 GANKK235 pKa = 10.03 AGAANSSAINAYY247 pKa = 10.37 GDD249 pKa = 3.63 GALADD254 pKa = 4.02 AFGMYY259 pKa = 10.35 AFSPYY264 pKa = 10.26 GDD266 pKa = 4.01 AYY268 pKa = 8.94 TINVGSIHH276 pKa = 6.15 VAADD280 pKa = 3.4 DD281 pKa = 3.94 TAVGGSSVALAGYY294 pKa = 8.73 AANEE298 pKa = 3.91 NRR300 pKa = 11.84 AGATIDD306 pKa = 3.35 VTGFDD311 pKa = 3.53 ATGLYY316 pKa = 10.08 AASLYY321 pKa = 8.54 GTAVNLNAGTITVAGVDD338 pKa = 3.58 ATGLEE343 pKa = 4.59 SYY345 pKa = 10.34 SVYY348 pKa = 11.12 GNAFAQNDD356 pKa = 3.95 GTLKK360 pKa = 10.42 VDD362 pKa = 3.29 GDD364 pKa = 4.27 FAQGAWAHH372 pKa = 5.58 SAYY375 pKa = 10.47 GSATTTNGATGSVNANGYY393 pKa = 9.29 YY394 pKa = 10.2 GAAGLIAGAYY404 pKa = 9.64 NGTATITNAGNASASSAAGIARR426 pKa = 11.84 GAFAYY431 pKa = 10.46 GDD433 pKa = 3.6 VAIVDD438 pKa = 3.65 NSGALSADD446 pKa = 3.43 GFTQAIGIDD455 pKa = 3.52 AEE457 pKa = 4.68 GNRR460 pKa = 11.84 SVDD463 pKa = 3.61 VVNSGSVVATSNGDD477 pKa = 3.44 AFGVWATSYY486 pKa = 11.84 GDD488 pKa = 3.43 TTVVNTASGSIDD500 pKa = 2.83 VDD502 pKa = 4.28 AINGIAYY509 pKa = 9.44 GIYY512 pKa = 10.05 AGGYY516 pKa = 8.49 DD517 pKa = 3.18 AYY519 pKa = 10.47 VRR521 pKa = 11.84 NWATVDD527 pKa = 3.39 VSSTYY532 pKa = 11.28 GDD534 pKa = 4.05 AIGIGVAGVYY544 pKa = 10.42 AFAEE548 pKa = 4.12 NDD550 pKa = 3.66 SLVNATGGTAGVGMLGYY567 pKa = 10.74 GVVTSDD573 pKa = 3.98 LVNGGTINATATGTGAFAAGVLSIGDD599 pKa = 3.66 GASALNTGTITATGAFATGIDD620 pKa = 3.7 VRR622 pKa = 11.84 GTTSGEE628 pKa = 4.07 AINDD632 pKa = 3.98 NTVHH636 pKa = 7.25 AYY638 pKa = 10.22 GGDD641 pKa = 3.39 VTGISVSAMGQGGNANVVNYY661 pKa = 10.79 GNVTAEE667 pKa = 3.93 GDD669 pKa = 3.86 LFAVGAAAIARR680 pKa = 11.84 GYY682 pKa = 10.15 LGNASIYY689 pKa = 10.88 NGGNVYY695 pKa = 10.76 AGAKK699 pKa = 9.53 YY700 pKa = 9.98 GAAQGLVASADD711 pKa = 3.2 IAASIDD717 pKa = 3.59 NQGQLITVGGDD728 pKa = 2.9 SAYY731 pKa = 10.73 GMLALTADD739 pKa = 3.53 GDD741 pKa = 4.26 INVTNGGQMATVAMNRR757 pKa = 11.84 GYY759 pKa = 11.02 GIAYY763 pKa = 9.48 GIATQSNNGLISITNDD779 pKa = 2.64 GLMVTVGEE787 pKa = 4.47 GTAMGVYY794 pKa = 10.16 AATKK798 pKa = 8.37 WNDD801 pKa = 3.11 VEE803 pKa = 4.58 IEE805 pKa = 4.27 NNGQMQAVAKK815 pKa = 10.21 YY816 pKa = 9.51 GAATGAIAITYY827 pKa = 8.52 TGDD830 pKa = 3.61 ANVSNSEE837 pKa = 4.07 YY838 pKa = 10.93 GQVLVQGAYY847 pKa = 9.7 AASGLRR853 pKa = 11.84 AISLYY858 pKa = 11.14 GDD860 pKa = 3.56 ANVTNDD866 pKa = 3.71 GDD868 pKa = 3.92 IEE870 pKa = 4.58 TMGGVLDD877 pKa = 4.39 RR878 pKa = 11.84 GITSRR883 pKa = 11.84 AFYY886 pKa = 10.63 GGTATITNNGTITTDD901 pKa = 2.89 GGYY904 pKa = 10.26 SSYY907 pKa = 11.24 GVWARR912 pKa = 11.84 SGAGDD917 pKa = 3.9 AVVHH921 pKa = 6.05 NNGAIVSTSDD931 pKa = 2.93 YY932 pKa = 10.21 GVAYY936 pKa = 9.79 GALAGGAYY944 pKa = 10.13 AISIEE949 pKa = 4.13 NTGDD953 pKa = 2.66 IRR955 pKa = 11.84 AYY957 pKa = 10.52 AGDD960 pKa = 3.57 TAAGVLAYY968 pKa = 10.66 SLGGDD973 pKa = 3.22 ATVTNTDD980 pKa = 2.5 TGYY983 pKa = 11.51 LLGVSNNAAYY993 pKa = 9.45 GAQVIGAVSATLTNDD1008 pKa = 3.2 GTIVASGYY1016 pKa = 10.34 NLADD1020 pKa = 3.28 AVFASGATVYY1030 pKa = 10.68 VSNTGSLSADD1040 pKa = 3.28 ADD1042 pKa = 3.35 GWAAGVEE1049 pKa = 4.21 ATGGDD1054 pKa = 3.56 IEE1056 pKa = 5.06 LVSHH1060 pKa = 6.76 TGADD1064 pKa = 3.11 ITAYY1068 pKa = 9.91 AYY1070 pKa = 10.36 SGRR1073 pKa = 11.84 ATGMFASADD1082 pKa = 3.25 NDD1084 pKa = 3.42 ATVRR1088 pKa = 11.84 NGGDD1092 pKa = 3.79 LDD1094 pKa = 3.86 ASSYY1098 pKa = 11.44 SGDD1101 pKa = 3.43 AYY1103 pKa = 10.89 GLFAQADD1110 pKa = 3.99 GNAILTNGGALTATSVQGNAFGMAGVGYY1138 pKa = 9.98 NADD1141 pKa = 3.97 LSQSATITVSGNGTAVGIEE1160 pKa = 4.24 GVGYY1164 pKa = 10.28 AHH1166 pKa = 7.56 ANIVNNGDD1174 pKa = 3.29 VDD1176 pKa = 3.8 VTAASGIAAGLYY1188 pKa = 8.74 TYY1190 pKa = 10.47 SVHH1193 pKa = 7.33 DD1194 pKa = 3.59 TRR1196 pKa = 11.84 QVNNGTIDD1204 pKa = 3.24 VHH1206 pKa = 5.54 ATSGRR1211 pKa = 11.84 AFGMYY1216 pKa = 9.92 AYY1218 pKa = 10.21 GFDD1221 pKa = 3.98 DD1222 pKa = 4.67 VEE1224 pKa = 4.45 VLNFAGRR1231 pKa = 11.84 SIDD1234 pKa = 3.5 SSVDD1238 pKa = 2.79 NGTAYY1243 pKa = 10.73 GMFGYY1248 pKa = 10.16 GARR1251 pKa = 11.84 TILTNDD1257 pKa = 3.2 GSVTATANSGQAIGLLGIGYY1277 pKa = 7.52 EE1278 pKa = 4.31 TSNVTNTATGSIVASGTSAFGLIASGDD1305 pKa = 3.58 VTTATNAGAITVHH1318 pKa = 6.3 GGDD1321 pKa = 3.75 VGVALQGVGMYY1332 pKa = 10.42 DD1333 pKa = 3.5 GSTVSIDD1340 pKa = 3.51 NTGTLYY1346 pKa = 11.33 ANVNNKK1352 pKa = 9.72 YY1353 pKa = 8.41 GTSAGIVGSAGGDD1366 pKa = 3.14 VDD1368 pKa = 3.57 ITNGGSVEE1376 pKa = 3.95 AHH1378 pKa = 6.28 NGRR1381 pKa = 11.84 VATGVVGYY1389 pKa = 9.47 SEE1391 pKa = 4.52 YY1392 pKa = 11.61 GNVTIGNTGDD1402 pKa = 2.88 IAAYY1406 pKa = 10.03 GGFFNGRR1413 pKa = 11.84 GVGIDD1418 pKa = 2.98 ATTYY1422 pKa = 11.02 DD1423 pKa = 3.77 GDD1425 pKa = 3.78 IAIDD1429 pKa = 3.58 SDD1431 pKa = 4.17 GSILATGKK1439 pKa = 10.14 YY1440 pKa = 10.35 ASGINAIADD1449 pKa = 3.91 GGDD1452 pKa = 3.33 VTVVNGGAITAATLGRR1468 pKa = 11.84 QGLGILATSYY1478 pKa = 11.25 DD1479 pKa = 3.62 GAVSVTNSGTIDD1491 pKa = 3.48 VGVGSPVTGAMVMGVVAGSEE1511 pKa = 4.03 YY1512 pKa = 11.29 GDD1514 pKa = 3.59 ASIHH1518 pKa = 5.63 NLAGATVTANNEE1530 pKa = 3.6 YY1531 pKa = 10.88 GMAGGLYY1538 pKa = 10.35 GYY1540 pKa = 9.17 GAHH1543 pKa = 6.36 LTIDD1547 pKa = 3.24 NGGDD1551 pKa = 3.45 VLVTGGPAAVGVAFDD1566 pKa = 4.89 ALDD1569 pKa = 3.52 ATIVNTGSITVSQLGGSNTPPPLAVEE1595 pKa = 4.23 EE1596 pKa = 4.61 GNGIGIAGFVAEE1608 pKa = 5.14 GGLAHH1613 pKa = 5.86 VTNDD1617 pKa = 3.2 GAISVTAHH1625 pKa = 6.6 DD1626 pKa = 4.17 VALGIQVMGDD1636 pKa = 3.21 GDD1638 pKa = 4.08 ILIDD1642 pKa = 3.61 GTGTISVVSSYY1653 pKa = 11.76 AEE1655 pKa = 4.3 AIGIEE1660 pKa = 3.93 ARR1662 pKa = 11.84 GPNVDD1667 pKa = 3.51 VTNGGTTQVTGYY1679 pKa = 10.57 AFAAGIEE1686 pKa = 4.34 GSGDD1690 pKa = 3.36 VVNAGNTGHH1699 pKa = 6.41 MMVNVTGMEE1708 pKa = 3.72 QDD1710 pKa = 3.26 AVGIAVLASTTADD1723 pKa = 3.21 IDD1725 pKa = 3.81 NQGDD1729 pKa = 3.94 LTVTAASGDD1738 pKa = 3.69 AYY1740 pKa = 10.52 GASAYY1745 pKa = 10.62 SDD1747 pKa = 3.33 TGVASIGNGGSIAVTANYY1765 pKa = 9.97 DD1766 pKa = 3.23 AVAIFALGATANVDD1780 pKa = 3.25 NTGDD1784 pKa = 3.19 ITATAGSHH1792 pKa = 7.23 AIGLFANGDD1801 pKa = 3.68 TVDD1804 pKa = 3.94 AFNGGHH1810 pKa = 5.97 ATATATGADD1819 pKa = 3.27 GMAYY1823 pKa = 9.27 GARR1826 pKa = 11.84 VSGEE1830 pKa = 3.83 DD1831 pKa = 3.23 VTFGNDD1837 pKa = 3.07 GTLSAFAEE1845 pKa = 4.56 GDD1847 pKa = 3.3 GGIAIGALAAGGTLHH1862 pKa = 7.33 ADD1864 pKa = 3.21 NGGHH1868 pKa = 5.72 ITATSEE1874 pKa = 4.25 GAGGLAIGFGAYY1886 pKa = 10.03 AGGDD1890 pKa = 3.4 LFASNGGEE1898 pKa = 3.82 IYY1900 pKa = 10.62 AIAYY1904 pKa = 8.9 NGAAIGAIAGSYY1916 pKa = 10.76 DD1917 pKa = 3.19 GGNVTFGNSGTIAAHH1932 pKa = 6.51 AAGEE1936 pKa = 4.5 TIGLRR1941 pKa = 11.84 VGGDD1945 pKa = 3.07 ADD1947 pKa = 3.63 ATVTNSGTIGAIHH1960 pKa = 7.34 ADD1962 pKa = 3.59 YY1963 pKa = 11.11 AVALSLDD1970 pKa = 3.6 VFGNATVNNTGDD1982 pKa = 3.12 IVAQASEE1989 pKa = 4.19 EE1990 pKa = 4.14 GSIAIHH1996 pKa = 6.42 GGDD1999 pKa = 4.07 GFDD2002 pKa = 4.65 LVTNSGDD2009 pKa = 3.2 IVGALVTGAGNDD2021 pKa = 3.42 RR2022 pKa = 11.84 LTNAAGGTWTVRR2034 pKa = 11.84 GHH2036 pKa = 5.96 ATDD2039 pKa = 5.49 FGDD2042 pKa = 4.56 GDD2044 pKa = 4.09 DD2045 pKa = 5.3 AIVNAGTIVLHH2056 pKa = 6.91 DD2057 pKa = 4.32 AGIGLGGNSATGNSFANTGTLSILGDD2083 pKa = 3.57 STIDD2087 pKa = 3.25 MGTGSSVVTLTANPAAAPIAVLNPVAFTNNGTISFLDD2124 pKa = 4.05 GAPDD2128 pKa = 3.55 DD2129 pKa = 4.08 TLTIHH2134 pKa = 7.31 GDD2136 pKa = 3.52 FAGQGALNVDD2146 pKa = 3.55 VSLLNGASDD2155 pKa = 3.66 MLYY2158 pKa = 10.64 VDD2160 pKa = 5.51 GNFANNAVQSLNINVLDD2177 pKa = 3.91 MLTGATEE2184 pKa = 3.43 IDD2186 pKa = 3.47 IPVVVVTGEE2195 pKa = 4.05 SQANNVRR2202 pKa = 11.84 AGSIIAGNVNFNANNFLDD2220 pKa = 4.46 LKK2222 pKa = 10.48 VAVNPHH2228 pKa = 4.61 ITAANGAVDD2237 pKa = 4.26 MFSLTLGFGGLNDD2250 pKa = 3.68 TGALATAIVPGAHH2263 pKa = 6.48 SLMAAQVGTFRR2274 pKa = 11.84 QRR2276 pKa = 11.84 MGVFSQLGDD2285 pKa = 3.54 SDD2287 pKa = 3.08 KK2288 pKa = 11.26 GAWVRR2293 pKa = 11.84 VFGDD2297 pKa = 3.17 KK2298 pKa = 10.29 GTINPEE2304 pKa = 3.8 AQLDD2308 pKa = 3.95 NLPASSNFAFDD2319 pKa = 3.21 QTNQGVEE2326 pKa = 3.66 AGYY2329 pKa = 10.27 NALITDD2335 pKa = 4.32 GFYY2338 pKa = 10.23 IGASLSKK2345 pKa = 10.87 SRR2347 pKa = 11.84 GKK2349 pKa = 10.13 QDD2351 pKa = 3.41 LANGFGDD2358 pKa = 4.69 DD2359 pKa = 5.46 DD2360 pKa = 4.85 IDD2362 pKa = 3.72 GTTLGGYY2369 pKa = 7.49 LTWLGQNGMYY2379 pKa = 10.65 ADD2381 pKa = 3.66 VSYY2384 pKa = 11.17 RR2385 pKa = 11.84 WMHH2388 pKa = 6.61 FDD2390 pKa = 4.96 ADD2392 pKa = 3.96 MTSLGGVRR2400 pKa = 11.84 EE2401 pKa = 4.16 VGGDD2405 pKa = 3.0 AGAFNAEE2412 pKa = 4.46 LGWNIWTSAGGLKK2425 pKa = 10.15 LVPQVQYY2432 pKa = 11.03 TRR2434 pKa = 11.84 TTVEE2438 pKa = 3.61 NVDD2441 pKa = 4.28 RR2442 pKa = 11.84 IEE2444 pKa = 5.03 GDD2446 pKa = 3.21 LADD2449 pKa = 4.35 FVSDD2453 pKa = 3.61 GGTSSRR2459 pKa = 11.84 ARR2461 pKa = 11.84 LGLEE2465 pKa = 3.74 MEE2467 pKa = 4.32 QTFDD2471 pKa = 3.77 SASGTKK2477 pKa = 6.48 WTPYY2481 pKa = 9.59 GVLSIVRR2488 pKa = 11.84 EE2489 pKa = 4.12 FDD2491 pKa = 3.38 GEE2493 pKa = 4.5 TSFTVADD2500 pKa = 3.99 TFTGRR2505 pKa = 11.84 TTTEE2509 pKa = 3.99 GTSGQVEE2516 pKa = 4.23 FGVNAKK2522 pKa = 9.87 IGQRR2526 pKa = 11.84 VDD2528 pKa = 2.5 VWGGLNYY2535 pKa = 9.5 MDD2537 pKa = 5.12 GGAIDD2542 pKa = 4.75 GVWGGQVGIRR2552 pKa = 11.84 YY2553 pKa = 6.63 TWW2555 pKa = 2.99
Molecular weight: 252.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.757
IPC_protein 3.834
Toseland 3.592
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.656
Grimsley 3.49
Solomon 3.834
Lehninger 3.795
Nozaki 3.935
DTASelect 4.253
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.961
Patrickios 0.515
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A0A2WJJ7|A0A0A2WJJ7_9GAMM Beta-glucosidase OS=Lysobacter dokdonensis DS-58 OX=1300345 GN=LF41_887 PE=3 SV=1
MM1 pKa = 7.54 HH2 pKa = 7.39 GGGFRR7 pKa = 11.84 KK8 pKa = 9.53 RR9 pKa = 11.84 GRR11 pKa = 11.84 MIPARR16 pKa = 11.84 RR17 pKa = 11.84 ATSRR21 pKa = 11.84 RR22 pKa = 11.84 AGRR25 pKa = 11.84 FGSAKK30 pKa = 9.76 SSHH33 pKa = 5.76 GAAARR38 pKa = 11.84 GRR40 pKa = 3.76
Molecular weight: 4.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.466
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.544
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.281
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3154
0
3154
998534
37
2555
316.6
34.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.134 ± 0.062
0.853 ± 0.014
6.257 ± 0.039
5.214 ± 0.046
3.539 ± 0.027
8.411 ± 0.051
2.297 ± 0.022
4.204 ± 0.03
3.03 ± 0.043
10.1 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.314 ± 0.022
2.54 ± 0.034
5.29 ± 0.033
3.434 ± 0.027
7.746 ± 0.054
4.863 ± 0.034
5.21 ± 0.039
7.683 ± 0.04
1.601 ± 0.024
2.279 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here