Lysobacter dokdonensis DS-58

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; Lysobacter dokdonensis

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A2WN33|A0A0A2WN33_9GAMM tRNA dimethylallyltransferase OS=Lysobacter dokdonensis DS-58 OX=1300345 GN=miaA PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.11NDD4 pKa = 3.27RR5 pKa = 11.84TRR7 pKa = 11.84ASRR10 pKa = 11.84KK11 pKa = 8.07AARR14 pKa = 11.84RR15 pKa = 11.84YY16 pKa = 9.32NVSPVARR23 pKa = 11.84AVRR26 pKa = 11.84SVLTLSTAALLFSGTGAVLAKK47 pKa = 10.53SPVLAPLPVEE57 pKa = 4.17RR58 pKa = 11.84VGLSHH63 pKa = 7.0EE64 pKa = 4.17FAKK67 pKa = 10.99VVDD70 pKa = 4.24LTRR73 pKa = 11.84VADD76 pKa = 3.43EE77 pKa = 4.14WTPKK81 pKa = 10.58SVVDD85 pKa = 5.14EE86 pKa = 4.49EE87 pKa = 4.47FQSLAAPIGASEE99 pKa = 4.26ATMFAAGAQPEE110 pKa = 4.51SLGVVDD116 pKa = 6.29DD117 pKa = 4.0PTILPGSFHH126 pKa = 7.37PGPGGVSGGDD136 pKa = 3.28DD137 pKa = 2.95AVLVNIGTGGNWTMVDD153 pKa = 4.07GYY155 pKa = 11.45APAPVVSNTGFADD168 pKa = 4.14GAFFGTADD176 pKa = 3.22SVTVRR181 pKa = 11.84NYY183 pKa = 9.86WDD185 pKa = 3.69GLSVTGYY192 pKa = 7.4TWAAGIEE199 pKa = 4.28VEE201 pKa = 5.21SGDD204 pKa = 3.53EE205 pKa = 4.01GLIGSYY211 pKa = 10.74DD212 pKa = 4.52AITATATGVDD222 pKa = 4.01GVAFGLYY229 pKa = 10.21VYY231 pKa = 10.01GANKK235 pKa = 10.03AGAANSSAINAYY247 pKa = 10.37GDD249 pKa = 3.63GALADD254 pKa = 4.02AFGMYY259 pKa = 10.35AFSPYY264 pKa = 10.26GDD266 pKa = 4.01AYY268 pKa = 8.94TINVGSIHH276 pKa = 6.15VAADD280 pKa = 3.4DD281 pKa = 3.94TAVGGSSVALAGYY294 pKa = 8.73AANEE298 pKa = 3.91NRR300 pKa = 11.84AGATIDD306 pKa = 3.35VTGFDD311 pKa = 3.53ATGLYY316 pKa = 10.08AASLYY321 pKa = 8.54GTAVNLNAGTITVAGVDD338 pKa = 3.58ATGLEE343 pKa = 4.59SYY345 pKa = 10.34SVYY348 pKa = 11.12GNAFAQNDD356 pKa = 3.95GTLKK360 pKa = 10.42VDD362 pKa = 3.29GDD364 pKa = 4.27FAQGAWAHH372 pKa = 5.58SAYY375 pKa = 10.47GSATTTNGATGSVNANGYY393 pKa = 9.29YY394 pKa = 10.2GAAGLIAGAYY404 pKa = 9.64NGTATITNAGNASASSAAGIARR426 pKa = 11.84GAFAYY431 pKa = 10.46GDD433 pKa = 3.6VAIVDD438 pKa = 3.65NSGALSADD446 pKa = 3.43GFTQAIGIDD455 pKa = 3.52AEE457 pKa = 4.68GNRR460 pKa = 11.84SVDD463 pKa = 3.61VVNSGSVVATSNGDD477 pKa = 3.44AFGVWATSYY486 pKa = 11.84GDD488 pKa = 3.43TTVVNTASGSIDD500 pKa = 2.83VDD502 pKa = 4.28AINGIAYY509 pKa = 9.44GIYY512 pKa = 10.05AGGYY516 pKa = 8.49DD517 pKa = 3.18AYY519 pKa = 10.47VRR521 pKa = 11.84NWATVDD527 pKa = 3.39VSSTYY532 pKa = 11.28GDD534 pKa = 4.05AIGIGVAGVYY544 pKa = 10.42AFAEE548 pKa = 4.12NDD550 pKa = 3.66SLVNATGGTAGVGMLGYY567 pKa = 10.74GVVTSDD573 pKa = 3.98LVNGGTINATATGTGAFAAGVLSIGDD599 pKa = 3.66GASALNTGTITATGAFATGIDD620 pKa = 3.7VRR622 pKa = 11.84GTTSGEE628 pKa = 4.07AINDD632 pKa = 3.98NTVHH636 pKa = 7.25AYY638 pKa = 10.22GGDD641 pKa = 3.39VTGISVSAMGQGGNANVVNYY661 pKa = 10.79GNVTAEE667 pKa = 3.93GDD669 pKa = 3.86LFAVGAAAIARR680 pKa = 11.84GYY682 pKa = 10.15LGNASIYY689 pKa = 10.88NGGNVYY695 pKa = 10.76AGAKK699 pKa = 9.53YY700 pKa = 9.98GAAQGLVASADD711 pKa = 3.2IAASIDD717 pKa = 3.59NQGQLITVGGDD728 pKa = 2.9SAYY731 pKa = 10.73GMLALTADD739 pKa = 3.53GDD741 pKa = 4.26INVTNGGQMATVAMNRR757 pKa = 11.84GYY759 pKa = 11.02GIAYY763 pKa = 9.48GIATQSNNGLISITNDD779 pKa = 2.64GLMVTVGEE787 pKa = 4.47GTAMGVYY794 pKa = 10.16AATKK798 pKa = 8.37WNDD801 pKa = 3.11VEE803 pKa = 4.58IEE805 pKa = 4.27NNGQMQAVAKK815 pKa = 10.21YY816 pKa = 9.51GAATGAIAITYY827 pKa = 8.52TGDD830 pKa = 3.61ANVSNSEE837 pKa = 4.07YY838 pKa = 10.93GQVLVQGAYY847 pKa = 9.7AASGLRR853 pKa = 11.84AISLYY858 pKa = 11.14GDD860 pKa = 3.56ANVTNDD866 pKa = 3.71GDD868 pKa = 3.92IEE870 pKa = 4.58TMGGVLDD877 pKa = 4.39RR878 pKa = 11.84GITSRR883 pKa = 11.84AFYY886 pKa = 10.63GGTATITNNGTITTDD901 pKa = 2.89GGYY904 pKa = 10.26SSYY907 pKa = 11.24GVWARR912 pKa = 11.84SGAGDD917 pKa = 3.9AVVHH921 pKa = 6.05NNGAIVSTSDD931 pKa = 2.93YY932 pKa = 10.21GVAYY936 pKa = 9.79GALAGGAYY944 pKa = 10.13AISIEE949 pKa = 4.13NTGDD953 pKa = 2.66IRR955 pKa = 11.84AYY957 pKa = 10.52AGDD960 pKa = 3.57TAAGVLAYY968 pKa = 10.66SLGGDD973 pKa = 3.22ATVTNTDD980 pKa = 2.5TGYY983 pKa = 11.51LLGVSNNAAYY993 pKa = 9.45GAQVIGAVSATLTNDD1008 pKa = 3.2GTIVASGYY1016 pKa = 10.34NLADD1020 pKa = 3.28AVFASGATVYY1030 pKa = 10.68VSNTGSLSADD1040 pKa = 3.28ADD1042 pKa = 3.35GWAAGVEE1049 pKa = 4.21ATGGDD1054 pKa = 3.56IEE1056 pKa = 5.06LVSHH1060 pKa = 6.76TGADD1064 pKa = 3.11ITAYY1068 pKa = 9.91AYY1070 pKa = 10.36SGRR1073 pKa = 11.84ATGMFASADD1082 pKa = 3.25NDD1084 pKa = 3.42ATVRR1088 pKa = 11.84NGGDD1092 pKa = 3.79LDD1094 pKa = 3.86ASSYY1098 pKa = 11.44SGDD1101 pKa = 3.43AYY1103 pKa = 10.89GLFAQADD1110 pKa = 3.99GNAILTNGGALTATSVQGNAFGMAGVGYY1138 pKa = 9.98NADD1141 pKa = 3.97LSQSATITVSGNGTAVGIEE1160 pKa = 4.24GVGYY1164 pKa = 10.28AHH1166 pKa = 7.56ANIVNNGDD1174 pKa = 3.29VDD1176 pKa = 3.8VTAASGIAAGLYY1188 pKa = 8.74TYY1190 pKa = 10.47SVHH1193 pKa = 7.33DD1194 pKa = 3.59TRR1196 pKa = 11.84QVNNGTIDD1204 pKa = 3.24VHH1206 pKa = 5.54ATSGRR1211 pKa = 11.84AFGMYY1216 pKa = 9.92AYY1218 pKa = 10.21GFDD1221 pKa = 3.98DD1222 pKa = 4.67VEE1224 pKa = 4.45VLNFAGRR1231 pKa = 11.84SIDD1234 pKa = 3.5SSVDD1238 pKa = 2.79NGTAYY1243 pKa = 10.73GMFGYY1248 pKa = 10.16GARR1251 pKa = 11.84TILTNDD1257 pKa = 3.2GSVTATANSGQAIGLLGIGYY1277 pKa = 7.52EE1278 pKa = 4.31TSNVTNTATGSIVASGTSAFGLIASGDD1305 pKa = 3.58VTTATNAGAITVHH1318 pKa = 6.3GGDD1321 pKa = 3.75VGVALQGVGMYY1332 pKa = 10.42DD1333 pKa = 3.5GSTVSIDD1340 pKa = 3.51NTGTLYY1346 pKa = 11.33ANVNNKK1352 pKa = 9.72YY1353 pKa = 8.41GTSAGIVGSAGGDD1366 pKa = 3.14VDD1368 pKa = 3.57ITNGGSVEE1376 pKa = 3.95AHH1378 pKa = 6.28NGRR1381 pKa = 11.84VATGVVGYY1389 pKa = 9.47SEE1391 pKa = 4.52YY1392 pKa = 11.61GNVTIGNTGDD1402 pKa = 2.88IAAYY1406 pKa = 10.03GGFFNGRR1413 pKa = 11.84GVGIDD1418 pKa = 2.98ATTYY1422 pKa = 11.02DD1423 pKa = 3.77GDD1425 pKa = 3.78IAIDD1429 pKa = 3.58SDD1431 pKa = 4.17GSILATGKK1439 pKa = 10.14YY1440 pKa = 10.35ASGINAIADD1449 pKa = 3.91GGDD1452 pKa = 3.33VTVVNGGAITAATLGRR1468 pKa = 11.84QGLGILATSYY1478 pKa = 11.25DD1479 pKa = 3.62GAVSVTNSGTIDD1491 pKa = 3.48VGVGSPVTGAMVMGVVAGSEE1511 pKa = 4.03YY1512 pKa = 11.29GDD1514 pKa = 3.59ASIHH1518 pKa = 5.63NLAGATVTANNEE1530 pKa = 3.6YY1531 pKa = 10.88GMAGGLYY1538 pKa = 10.35GYY1540 pKa = 9.17GAHH1543 pKa = 6.36LTIDD1547 pKa = 3.24NGGDD1551 pKa = 3.45VLVTGGPAAVGVAFDD1566 pKa = 4.89ALDD1569 pKa = 3.52ATIVNTGSITVSQLGGSNTPPPLAVEE1595 pKa = 4.23EE1596 pKa = 4.61GNGIGIAGFVAEE1608 pKa = 5.14GGLAHH1613 pKa = 5.86VTNDD1617 pKa = 3.2GAISVTAHH1625 pKa = 6.6DD1626 pKa = 4.17VALGIQVMGDD1636 pKa = 3.21GDD1638 pKa = 4.08ILIDD1642 pKa = 3.61GTGTISVVSSYY1653 pKa = 11.76AEE1655 pKa = 4.3AIGIEE1660 pKa = 3.93ARR1662 pKa = 11.84GPNVDD1667 pKa = 3.51VTNGGTTQVTGYY1679 pKa = 10.57AFAAGIEE1686 pKa = 4.34GSGDD1690 pKa = 3.36VVNAGNTGHH1699 pKa = 6.41MMVNVTGMEE1708 pKa = 3.72QDD1710 pKa = 3.26AVGIAVLASTTADD1723 pKa = 3.21IDD1725 pKa = 3.81NQGDD1729 pKa = 3.94LTVTAASGDD1738 pKa = 3.69AYY1740 pKa = 10.52GASAYY1745 pKa = 10.62SDD1747 pKa = 3.33TGVASIGNGGSIAVTANYY1765 pKa = 9.97DD1766 pKa = 3.23AVAIFALGATANVDD1780 pKa = 3.25NTGDD1784 pKa = 3.19ITATAGSHH1792 pKa = 7.23AIGLFANGDD1801 pKa = 3.68TVDD1804 pKa = 3.94AFNGGHH1810 pKa = 5.97ATATATGADD1819 pKa = 3.27GMAYY1823 pKa = 9.27GARR1826 pKa = 11.84VSGEE1830 pKa = 3.83DD1831 pKa = 3.23VTFGNDD1837 pKa = 3.07GTLSAFAEE1845 pKa = 4.56GDD1847 pKa = 3.3GGIAIGALAAGGTLHH1862 pKa = 7.33ADD1864 pKa = 3.21NGGHH1868 pKa = 5.72ITATSEE1874 pKa = 4.25GAGGLAIGFGAYY1886 pKa = 10.03AGGDD1890 pKa = 3.4LFASNGGEE1898 pKa = 3.82IYY1900 pKa = 10.62AIAYY1904 pKa = 8.9NGAAIGAIAGSYY1916 pKa = 10.76DD1917 pKa = 3.19GGNVTFGNSGTIAAHH1932 pKa = 6.51AAGEE1936 pKa = 4.5TIGLRR1941 pKa = 11.84VGGDD1945 pKa = 3.07ADD1947 pKa = 3.63ATVTNSGTIGAIHH1960 pKa = 7.34ADD1962 pKa = 3.59YY1963 pKa = 11.11AVALSLDD1970 pKa = 3.6VFGNATVNNTGDD1982 pKa = 3.12IVAQASEE1989 pKa = 4.19EE1990 pKa = 4.14GSIAIHH1996 pKa = 6.42GGDD1999 pKa = 4.07GFDD2002 pKa = 4.65LVTNSGDD2009 pKa = 3.2IVGALVTGAGNDD2021 pKa = 3.42RR2022 pKa = 11.84LTNAAGGTWTVRR2034 pKa = 11.84GHH2036 pKa = 5.96ATDD2039 pKa = 5.49FGDD2042 pKa = 4.56GDD2044 pKa = 4.09DD2045 pKa = 5.3AIVNAGTIVLHH2056 pKa = 6.91DD2057 pKa = 4.32AGIGLGGNSATGNSFANTGTLSILGDD2083 pKa = 3.57STIDD2087 pKa = 3.25MGTGSSVVTLTANPAAAPIAVLNPVAFTNNGTISFLDD2124 pKa = 4.05GAPDD2128 pKa = 3.55DD2129 pKa = 4.08TLTIHH2134 pKa = 7.31GDD2136 pKa = 3.52FAGQGALNVDD2146 pKa = 3.55VSLLNGASDD2155 pKa = 3.66MLYY2158 pKa = 10.64VDD2160 pKa = 5.51GNFANNAVQSLNINVLDD2177 pKa = 3.91MLTGATEE2184 pKa = 3.43IDD2186 pKa = 3.47IPVVVVTGEE2195 pKa = 4.05SQANNVRR2202 pKa = 11.84AGSIIAGNVNFNANNFLDD2220 pKa = 4.46LKK2222 pKa = 10.48VAVNPHH2228 pKa = 4.61ITAANGAVDD2237 pKa = 4.26MFSLTLGFGGLNDD2250 pKa = 3.68TGALATAIVPGAHH2263 pKa = 6.48SLMAAQVGTFRR2274 pKa = 11.84QRR2276 pKa = 11.84MGVFSQLGDD2285 pKa = 3.54SDD2287 pKa = 3.08KK2288 pKa = 11.26GAWVRR2293 pKa = 11.84VFGDD2297 pKa = 3.17KK2298 pKa = 10.29GTINPEE2304 pKa = 3.8AQLDD2308 pKa = 3.95NLPASSNFAFDD2319 pKa = 3.21QTNQGVEE2326 pKa = 3.66AGYY2329 pKa = 10.27NALITDD2335 pKa = 4.32GFYY2338 pKa = 10.23IGASLSKK2345 pKa = 10.87SRR2347 pKa = 11.84GKK2349 pKa = 10.13QDD2351 pKa = 3.41LANGFGDD2358 pKa = 4.69DD2359 pKa = 5.46DD2360 pKa = 4.85IDD2362 pKa = 3.72GTTLGGYY2369 pKa = 7.49LTWLGQNGMYY2379 pKa = 10.65ADD2381 pKa = 3.66VSYY2384 pKa = 11.17RR2385 pKa = 11.84WMHH2388 pKa = 6.61FDD2390 pKa = 4.96ADD2392 pKa = 3.96MTSLGGVRR2400 pKa = 11.84EE2401 pKa = 4.16VGGDD2405 pKa = 3.0AGAFNAEE2412 pKa = 4.46LGWNIWTSAGGLKK2425 pKa = 10.15LVPQVQYY2432 pKa = 11.03TRR2434 pKa = 11.84TTVEE2438 pKa = 3.61NVDD2441 pKa = 4.28RR2442 pKa = 11.84IEE2444 pKa = 5.03GDD2446 pKa = 3.21LADD2449 pKa = 4.35FVSDD2453 pKa = 3.61GGTSSRR2459 pKa = 11.84ARR2461 pKa = 11.84LGLEE2465 pKa = 3.74MEE2467 pKa = 4.32QTFDD2471 pKa = 3.77SASGTKK2477 pKa = 6.48WTPYY2481 pKa = 9.59GVLSIVRR2488 pKa = 11.84EE2489 pKa = 4.12FDD2491 pKa = 3.38GEE2493 pKa = 4.5TSFTVADD2500 pKa = 3.99TFTGRR2505 pKa = 11.84TTTEE2509 pKa = 3.99GTSGQVEE2516 pKa = 4.23FGVNAKK2522 pKa = 9.87IGQRR2526 pKa = 11.84VDD2528 pKa = 2.5VWGGLNYY2535 pKa = 9.5MDD2537 pKa = 5.12GGAIDD2542 pKa = 4.75GVWGGQVGIRR2552 pKa = 11.84YY2553 pKa = 6.63TWW2555 pKa = 2.99

Molecular weight:
252.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A2WJJ7|A0A0A2WJJ7_9GAMM Beta-glucosidase OS=Lysobacter dokdonensis DS-58 OX=1300345 GN=LF41_887 PE=3 SV=1
MM1 pKa = 7.54HH2 pKa = 7.39GGGFRR7 pKa = 11.84KK8 pKa = 9.53RR9 pKa = 11.84GRR11 pKa = 11.84MIPARR16 pKa = 11.84RR17 pKa = 11.84ATSRR21 pKa = 11.84RR22 pKa = 11.84AGRR25 pKa = 11.84FGSAKK30 pKa = 9.76SSHH33 pKa = 5.76GAAARR38 pKa = 11.84GRR40 pKa = 3.76

Molecular weight:
4.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3154

0

3154

998534

37

2555

316.6

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.134 ± 0.062

0.853 ± 0.014

6.257 ± 0.039

5.214 ± 0.046

3.539 ± 0.027

8.411 ± 0.051

2.297 ± 0.022

4.204 ± 0.03

3.03 ± 0.043

10.1 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.022

2.54 ± 0.034

5.29 ± 0.033

3.434 ± 0.027

7.746 ± 0.054

4.863 ± 0.034

5.21 ± 0.039

7.683 ± 0.04

1.601 ± 0.024

2.279 ± 0.026

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski