Penicillium vulpinum
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10245 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V6R2S4|A0A1V6R2S4_9EURO Uncharacterized protein OS=Penicillium vulpinum OX=29845 GN=PENVUL_c102G09685 PE=4 SV=1
QQ1 pKa = 7.03 SLSVHH6 pKa = 6.95 DD7 pKa = 4.45 PQLPPSTDD15 pKa = 2.23 WSQQYY20 pKa = 11.05 DD21 pKa = 3.21 WDD23 pKa = 4.08 FDD25 pKa = 4.75 GILQQSLSVHH35 pKa = 6.95 DD36 pKa = 4.4 PQLPPSTDD44 pKa = 2.78 RR45 pKa = 11.84 SQQYY49 pKa = 10.87 DD50 pKa = 3.01 WDD52 pKa = 4.01 FDD54 pKa = 4.75 GILQQSLSVHH64 pKa = 6.95 DD65 pKa = 4.4 PQLPPSTDD73 pKa = 3.0 RR74 pKa = 11.84 SQQCSSDD81 pKa = 4.56 LLQQDD86 pKa = 3.83 PLLL89 pKa = 4.91
Molecular weight: 10.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.63
IPC_protein 3.63
Toseland 3.401
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.706
Rodwell 3.465
Grimsley 3.312
Solomon 3.656
Lehninger 3.605
Nozaki 3.808
DTASelect 4.151
Thurlkill 3.49
EMBOSS 3.706
Sillero 3.77
Patrickios 1.901
IPC_peptide 3.643
IPC2_peptide 3.732
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A1V6SEP4|A0A1V6SEP4_9EURO PhoD domain-containing protein OS=Penicillium vulpinum OX=29845 GN=PENVUL_c001G08639 PE=4 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.11 WRR6 pKa = 11.84 KK7 pKa = 9.07 KK8 pKa = 6.93 RR9 pKa = 11.84 TRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 9.73 RR15 pKa = 11.84 KK16 pKa = 7.87 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.6 MRR21 pKa = 11.84 ARR23 pKa = 11.84 SKK25 pKa = 11.11
Molecular weight: 3.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10245
0
10245
5054469
8
6349
493.4
54.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.303 ± 0.021
1.257 ± 0.009
5.637 ± 0.015
6.15 ± 0.025
3.826 ± 0.015
6.678 ± 0.02
2.423 ± 0.009
5.258 ± 0.016
4.667 ± 0.02
9.025 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.27 ± 0.01
3.803 ± 0.01
6.003 ± 0.024
4.066 ± 0.015
5.876 ± 0.022
8.423 ± 0.025
5.985 ± 0.017
6.069 ± 0.018
1.475 ± 0.009
2.805 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here