Planctomicrobium piriforme
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5057 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I3HHL5|A0A1I3HHL5_9PLAN Uncharacterized protein OS=Planctomicrobium piriforme OX=1576369 GN=SAMN05421753_10869 PE=4 SV=1
MM1 pKa = 7.34 LRR3 pKa = 11.84 KK4 pKa = 9.68 SLSSLLWSAWGRR16 pKa = 11.84 LQAGSRR22 pKa = 11.84 NSGRR26 pKa = 11.84 GRR28 pKa = 11.84 RR29 pKa = 11.84 PLQRR33 pKa = 11.84 HH34 pKa = 4.06 SWNRR38 pKa = 11.84 SLSRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 QSGVPALEE53 pKa = 3.89 RR54 pKa = 11.84 LEE56 pKa = 4.59 ARR58 pKa = 11.84 QLLSATASGAEE69 pKa = 4.02 YY70 pKa = 10.25 RR71 pKa = 11.84 VNAYY75 pKa = 6.29 TTSIQSQPSMAMDD88 pKa = 3.45 SAGNYY93 pKa = 7.71 VVTWQSYY100 pKa = 8.16 GQDD103 pKa = 3.12 GDD105 pKa = 4.03 GYY107 pKa = 10.94 GIYY110 pKa = 10.35 AQRR113 pKa = 11.84 FNAAGVAQGSEE124 pKa = 4.26 FRR126 pKa = 11.84 VNTYY130 pKa = 5.47 TTYY133 pKa = 10.88 YY134 pKa = 10.01 QFNPSAAMDD143 pKa = 3.75 SDD145 pKa = 3.88 GDD147 pKa = 4.54 FVITWVTDD155 pKa = 3.47 QEE157 pKa = 4.69 GSSNDD162 pKa = 3.04 IYY164 pKa = 10.71 AQRR167 pKa = 11.84 YY168 pKa = 5.85 NALGEE173 pKa = 4.1 AQGTEE178 pKa = 4.16 FRR180 pKa = 11.84 VNTYY184 pKa = 7.54 TTDD187 pKa = 3.45 PQYY190 pKa = 11.45 SPSAAMDD197 pKa = 3.51 SDD199 pKa = 3.76 GDD201 pKa = 4.95 FVVTWQSYY209 pKa = 8.32 GQDD212 pKa = 3.12 GDD214 pKa = 4.03 GYY216 pKa = 10.94 GIYY219 pKa = 10.35 AQRR222 pKa = 11.84 FNAAGVAQGSEE233 pKa = 4.37 FQVNTYY239 pKa = 6.31 TTSYY243 pKa = 10.78 QYY245 pKa = 9.89 TASAAMDD252 pKa = 3.68 SDD254 pKa = 3.92 GDD256 pKa = 5.21 FVITWQSYY264 pKa = 7.9 GQDD267 pKa = 2.97 GSGYY271 pKa = 9.91 GIYY274 pKa = 9.89 AQRR277 pKa = 11.84 YY278 pKa = 5.86 NAAGVAQGSEE288 pKa = 4.44 FQVSSYY294 pKa = 7.25 TTNNQNSSSAAMDD307 pKa = 3.43 SDD309 pKa = 3.84 GDD311 pKa = 4.86 FVISWQSSGQDD322 pKa = 3.16 GSGYY326 pKa = 10.49 GVYY329 pKa = 10.57 AKK331 pKa = 10.21 RR332 pKa = 11.84 FNASGVAQGSEE343 pKa = 4.18 FRR345 pKa = 11.84 VNTYY349 pKa = 7.27 TTSSQYY355 pKa = 10.51 NASPAMNAAGNFVISWQSSGQDD377 pKa = 3.49 GNGLGIYY384 pKa = 8.55 AQYY387 pKa = 11.04 YY388 pKa = 7.9 NAAGVAQGSEE398 pKa = 4.35 FRR400 pKa = 11.84 ANSYY404 pKa = 6.77 TTNSQANPTAAMNSTGNFVIAWQSSGQDD432 pKa = 3.27 GSSNGIYY439 pKa = 8.55 SQRR442 pKa = 11.84 YY443 pKa = 8.22 SVGAPPTDD451 pKa = 3.02 ISLSANTVSEE461 pKa = 4.11 IASIGTTIGTLTATDD476 pKa = 3.79 ADD478 pKa = 4.05 SPEE481 pKa = 4.16 SFTYY485 pKa = 10.5 RR486 pKa = 11.84 LLTNVEE492 pKa = 4.42 SPANGLFLIDD502 pKa = 4.38 GDD504 pKa = 4.28 TLKK507 pKa = 10.07 TAGALNYY514 pKa = 9.24 EE515 pKa = 4.37 AVHH518 pKa = 5.97 QYY520 pKa = 10.99 SVDD523 pKa = 3.42 IEE525 pKa = 4.64 VTDD528 pKa = 3.67 STAQTFVKK536 pKa = 9.8 TFTITVTDD544 pKa = 3.79 SNEE547 pKa = 3.92 FAVSAVSDD555 pKa = 3.96 TNGDD559 pKa = 3.61 TNTIAEE565 pKa = 4.16 NAAAGTVGITASASDD580 pKa = 4.28 ADD582 pKa = 3.97 GTTNAVSYY590 pKa = 11.09 SLTDD594 pKa = 3.29 NAGGLFTIDD603 pKa = 2.99 STTGVVSTTGPLNYY617 pKa = 7.74 EE618 pKa = 4.02 TATSYY623 pKa = 10.77 TITVRR628 pKa = 11.84 AEE630 pKa = 3.89 SADD633 pKa = 3.62 GSFSAADD640 pKa = 3.41 FTITVTDD647 pKa = 3.35 IDD649 pKa = 4.32 EE650 pKa = 4.92 SDD652 pKa = 3.53 VSPITDD658 pKa = 3.44 SDD660 pKa = 4.07 SASNTIAEE668 pKa = 4.18 NAAAGTVGITASASDD683 pKa = 4.18 ADD685 pKa = 4.02 GTTNTVTYY693 pKa = 10.72 SLTDD697 pKa = 3.28 NAGGLFTIDD706 pKa = 2.98 SATGVVSTTGPLDD719 pKa = 3.46 YY720 pKa = 9.48 EE721 pKa = 4.51 TATSYY726 pKa = 11.12 SITVRR731 pKa = 11.84 AEE733 pKa = 3.83 SADD736 pKa = 3.6 GSFSTADD743 pKa = 3.17 FTIAVTDD750 pKa = 3.48 VDD752 pKa = 4.0 EE753 pKa = 5.68 SDD755 pKa = 3.5 VSSITDD761 pKa = 3.19 TDD763 pKa = 3.81 TADD766 pKa = 3.39 NTITEE771 pKa = 4.34 SADD774 pKa = 3.05 AGTVGITASASDD786 pKa = 4.18 ADD788 pKa = 4.02 GTTNTVTYY796 pKa = 10.72 SLTDD800 pKa = 3.28 NAGGLFTIDD809 pKa = 2.99 STTGVVSTTGPLNYY823 pKa = 7.74 EE824 pKa = 4.02 TATSYY829 pKa = 10.77 TITVRR834 pKa = 11.84 AEE836 pKa = 3.99 SEE838 pKa = 4.17 DD839 pKa = 3.84 GSFSAADD846 pKa = 3.51 FTVTVTDD853 pKa = 3.22 VDD855 pKa = 3.87 EE856 pKa = 5.7 SDD858 pKa = 3.59 VSPITDD864 pKa = 3.44 SDD866 pKa = 4.07 SASNTIAEE874 pKa = 4.32 NADD877 pKa = 3.32 AGTVGITASASDD889 pKa = 4.18 ADD891 pKa = 4.02 GTTNTVTYY899 pKa = 10.72 SLTDD903 pKa = 3.28 NAGGLFTIDD912 pKa = 2.99 STTGVVSTTGPLNYY926 pKa = 7.74 EE927 pKa = 4.02 TATSYY932 pKa = 10.77 TITVRR937 pKa = 11.84 AEE939 pKa = 3.99 SEE941 pKa = 4.15 DD942 pKa = 3.8 GSFSTTDD949 pKa = 3.28 FTIAVTDD956 pKa = 3.48 VDD958 pKa = 3.91 EE959 pKa = 5.53 SDD961 pKa = 3.43 VSGVTDD967 pKa = 3.75 TDD969 pKa = 3.7 STDD972 pKa = 3.12 NTISEE977 pKa = 4.15 NAAAGPVGITAFASDD992 pKa = 4.78 ADD994 pKa = 3.96 GTTNAVSYY1002 pKa = 11.09 SLTDD1006 pKa = 3.29 NAGGLFTIDD1015 pKa = 3.95 SISGVVSTTGPLDD1028 pKa = 3.46 YY1029 pKa = 9.48 EE1030 pKa = 4.51 TATSYY1035 pKa = 11.12 SITVRR1040 pKa = 11.84 AEE1042 pKa = 3.83 SADD1045 pKa = 3.48 GSFSIADD1052 pKa = 3.26 FTIAVTDD1059 pKa = 3.31 VDD1061 pKa = 4.33 EE1062 pKa = 5.36 FDD1064 pKa = 3.68 VSAIADD1070 pKa = 3.52 ADD1072 pKa = 3.92 AADD1075 pKa = 3.62 NTIAEE1080 pKa = 4.45 NADD1083 pKa = 3.59 AGPVGITASASDD1095 pKa = 3.76 SDD1097 pKa = 4.17 GATNTVTYY1105 pKa = 10.86 SLTDD1109 pKa = 3.48 DD1110 pKa = 3.84 AGGLFTIDD1118 pKa = 3.95 SISGVVSTTGPLNYY1132 pKa = 7.7 EE1133 pKa = 4.02 TATSYY1138 pKa = 11.1 SITVRR1143 pKa = 11.84 AEE1145 pKa = 3.83 SADD1148 pKa = 3.31 GSFSTEE1154 pKa = 3.47 NFTIAVTDD1162 pKa = 3.55 VNEE1165 pKa = 4.09 FDD1167 pKa = 3.63 VSDD1170 pKa = 4.93 VIDD1173 pKa = 3.9 TNCASNVIAEE1183 pKa = 4.22 NAAAGPIGITAFADD1197 pKa = 4.13 DD1198 pKa = 4.94 EE1199 pKa = 5.2 DD1200 pKa = 4.49 GTTNGVTYY1208 pKa = 10.88 SLTDD1212 pKa = 3.47 DD1213 pKa = 3.84 AGGLFTINSCTGVVSTTGPLDD1234 pKa = 3.4 YY1235 pKa = 9.46 EE1236 pKa = 4.7 TACSYY1241 pKa = 11.03 SITVRR1246 pKa = 11.84 AEE1248 pKa = 3.83 SADD1251 pKa = 3.5 GSFSMADD1258 pKa = 3.19 FTVNVLNVNEE1268 pKa = 4.45 SPVISGVTGSTSYY1281 pKa = 10.86 IEE1283 pKa = 4.03 NAAPRR1288 pKa = 11.84 ILTTDD1293 pKa = 3.51 GVINDD1298 pKa = 4.51 PDD1300 pKa = 4.09 GNFCGGSLTVRR1311 pKa = 11.84 FRR1313 pKa = 11.84 GFSEE1317 pKa = 3.85 TGDD1320 pKa = 3.49 RR1321 pKa = 11.84 LSILSVGTSTGQISVTGNDD1340 pKa = 2.98 VFYY1343 pKa = 11.1 GCTLLGTVTSDD1354 pKa = 3.06 GTGGQNLVIQLAAGADD1370 pKa = 3.61 NAGVQQLLRR1379 pKa = 11.84 SITYY1383 pKa = 10.31 ASVSEE1388 pKa = 4.4 NPSTTQRR1395 pKa = 11.84 RR1396 pKa = 11.84 VEE1398 pKa = 4.1 FVLKK1402 pKa = 10.74 DD1403 pKa = 3.78 GVGTASNVGAKK1414 pKa = 8.29 VVRR1417 pKa = 11.84 VTAVNDD1423 pKa = 3.52 KK1424 pKa = 10.64 PVITGVTGSTTYY1436 pKa = 10.44 TEE1438 pKa = 4.01 NAAPLVLATDD1448 pKa = 4.72 GVISDD1453 pKa = 4.81 LDD1455 pKa = 4.12 DD1456 pKa = 3.95 NLGEE1460 pKa = 4.28 GSLTVRR1466 pKa = 11.84 FNGFSEE1472 pKa = 4.47 ADD1474 pKa = 3.43 DD1475 pKa = 3.88 RR1476 pKa = 11.84 LSILSVGTGDD1486 pKa = 3.68 GQISVTGNDD1495 pKa = 3.0 VFYY1498 pKa = 10.94 GGSLLGTISSDD1509 pKa = 3.04 GTGGQNLVIQLASGASSEE1527 pKa = 5.05 AVQQLLRR1534 pKa = 11.84 SITYY1538 pKa = 10.31 ASVSEE1543 pKa = 4.38 NPNTTQRR1550 pKa = 11.84 RR1551 pKa = 11.84 VEE1553 pKa = 3.82 FALRR1557 pKa = 11.84 DD1558 pKa = 3.48 GDD1560 pKa = 4.03 GANSNVGARR1569 pKa = 11.84 IVKK1572 pKa = 9.54 VKK1574 pKa = 10.51 SVNDD1578 pKa = 3.55 KK1579 pKa = 10.76 PVITGVTGTATYY1591 pKa = 10.26 SKK1593 pKa = 10.54 SGLPIALATGGDD1605 pKa = 3.84 VSDD1608 pKa = 5.77 LDD1610 pKa = 3.66 WNMAGGSLTARR1621 pKa = 11.84 FNGFSEE1627 pKa = 4.58 ANDD1630 pKa = 3.63 RR1631 pKa = 11.84 LSIISGGTGTGEE1643 pKa = 3.97 INVSGNDD1650 pKa = 3.2 VYY1652 pKa = 11.82 YY1653 pKa = 10.5 EE1654 pKa = 4.23 GDD1656 pKa = 4.05 LIGSITSNGIGGQALTITLTAAATPDD1682 pKa = 3.51 AVRR1685 pKa = 11.84 HH1686 pKa = 5.6 LLHH1689 pKa = 6.52 SLTYY1693 pKa = 10.57 ASDD1696 pKa = 3.44 STTPTTTNRR1705 pKa = 11.84 RR1706 pKa = 11.84 VEE1708 pKa = 4.13 FLLTDD1713 pKa = 3.61 SSGAKK1718 pKa = 10.05 SNTAARR1724 pKa = 11.84 VVKK1727 pKa = 10.15 FVSS1730 pKa = 3.15
Molecular weight: 178.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.668
IPC_protein 3.719
Toseland 3.478
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.821
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.681
Sillero 3.846
Patrickios 1.138
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A1I3LYJ1|A0A1I3LYJ1_9PLAN Putative signal transducing protein OS=Planctomicrobium piriforme OX=1576369 GN=SAMN05421753_113106 PE=4 SV=1
MM1 pKa = 7.66 AVILLIALMSNRR13 pKa = 11.84 QPSMQQSSSVRR24 pKa = 11.84 STSAQTVSNRR34 pKa = 11.84 NAQTQRR40 pKa = 11.84 GPLRR44 pKa = 11.84 RR45 pKa = 11.84 LVGRR49 pKa = 11.84 MRR51 pKa = 11.84 NN52 pKa = 3.38
Molecular weight: 5.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5057
0
5057
1801768
27
9591
356.3
39.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.904 ± 0.037
1.18 ± 0.015
5.482 ± 0.029
5.907 ± 0.038
3.803 ± 0.023
7.752 ± 0.044
2.136 ± 0.016
4.895 ± 0.023
3.673 ± 0.033
10.345 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.017
3.209 ± 0.031
5.522 ± 0.031
4.446 ± 0.026
6.463 ± 0.043
6.282 ± 0.036
5.694 ± 0.054
7.24 ± 0.029
1.543 ± 0.017
2.387 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here