Staphylococcus phage IME1354_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JQA0|A0A1W6JQA0_9CAUD Protease-like protein OS=Staphylococcus phage IME1354_01 OX=1965493 PE=3 SV=1
MM1 pKa = 7.53AYY3 pKa = 9.51EE4 pKa = 4.24YY5 pKa = 10.02EE6 pKa = 4.18SDD8 pKa = 3.94VISEE12 pKa = 4.44LEE14 pKa = 4.0QMGADD19 pKa = 3.81YY20 pKa = 10.96QSNEE24 pKa = 4.17YY25 pKa = 10.47FNMSIEE31 pKa = 4.07VEE33 pKa = 4.12EE34 pKa = 4.87VYY36 pKa = 10.64RR37 pKa = 11.84KK38 pKa = 10.3AKK40 pKa = 10.31AYY42 pKa = 7.7EE43 pKa = 4.15KK44 pKa = 10.58LVEE47 pKa = 4.95LYY49 pKa = 9.17PQKK52 pKa = 11.13DD53 pKa = 4.16EE54 pKa = 4.28IPSDD58 pKa = 3.88EE59 pKa = 4.73FFMMMGYY66 pKa = 9.81IIKK69 pKa = 10.23EE70 pKa = 4.01LTDD73 pKa = 3.5DD74 pKa = 3.65EE75 pKa = 4.63RR76 pKa = 5.97

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JQB0|A0A1W6JQB0_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1354_01 OX=1965493 PE=4 SV=1
MM1 pKa = 7.74ADD3 pKa = 3.71EE4 pKa = 4.93IKK6 pKa = 10.95GFTIDD11 pKa = 5.15LGIDD15 pKa = 3.11TSDD18 pKa = 3.69IDD20 pKa = 4.1RR21 pKa = 11.84GMANLQRR28 pKa = 11.84KK29 pKa = 8.8LKK31 pKa = 9.62TADD34 pKa = 3.29AQMKK38 pKa = 10.46ANLSTFDD45 pKa = 3.68KK46 pKa = 11.15AEE48 pKa = 3.95KK49 pKa = 10.56SVDD52 pKa = 3.24KK53 pKa = 11.21FEE55 pKa = 4.73TEE57 pKa = 4.73LEE59 pKa = 4.12GLNKK63 pKa = 10.35KK64 pKa = 8.05LTQQGRR70 pKa = 11.84ASEE73 pKa = 4.06QSQKK77 pKa = 11.32KK78 pKa = 10.07LDD80 pKa = 3.76QLRR83 pKa = 11.84NAQDD87 pKa = 3.43NTNKK91 pKa = 9.98KK92 pKa = 8.3MKK94 pKa = 9.52EE95 pKa = 3.86AAVQAQNAKK104 pKa = 9.91SRR106 pKa = 11.84YY107 pKa = 8.43EE108 pKa = 4.07SLADD112 pKa = 5.24SYY114 pKa = 12.17DD115 pKa = 3.42KK116 pKa = 11.43LNNEE120 pKa = 4.31LKK122 pKa = 10.3EE123 pKa = 4.01HH124 pKa = 4.9QTNVKK129 pKa = 9.71NAQNAQKK136 pKa = 10.7QMQNTVTALSAKK148 pKa = 8.74MKK150 pKa = 9.63NAKK153 pKa = 10.14SSVDD157 pKa = 3.35GLQEE161 pKa = 4.81EE162 pKa = 4.77FDD164 pKa = 3.69QLNASGKK171 pKa = 9.97ASKK174 pKa = 10.61QEE176 pKa = 3.9LTSLGNQLAKK186 pKa = 10.71ARR188 pKa = 11.84AQYY191 pKa = 11.15NSLSNAVDD199 pKa = 3.62SAKK202 pKa = 10.33QDD204 pKa = 3.78LNEE207 pKa = 4.56SKK209 pKa = 10.91VATANAKK216 pKa = 10.33NEE218 pKa = 4.01LQNFSDD224 pKa = 3.93ANKK227 pKa = 10.39EE228 pKa = 3.95AMTSAKK234 pKa = 9.61TAMQSAKK241 pKa = 10.35QEE243 pKa = 4.04ANNAEE248 pKa = 4.16KK249 pKa = 10.53SYY251 pKa = 11.44ASLNRR256 pKa = 11.84EE257 pKa = 4.17VAQLPSKK264 pKa = 10.4LDD266 pKa = 3.38KK267 pKa = 11.21AEE269 pKa = 4.13KK270 pKa = 9.77EE271 pKa = 4.38VYY273 pKa = 10.22EE274 pKa = 4.1NALAYY279 pKa = 10.32NVLQNRR285 pKa = 11.84IDD287 pKa = 3.64EE288 pKa = 4.74ATDD291 pKa = 3.08EE292 pKa = 4.01MRR294 pKa = 11.84EE295 pKa = 3.87FHH297 pKa = 7.38RR298 pKa = 11.84EE299 pKa = 3.53QTKK302 pKa = 9.88FFGMGPAIAAMGQRR316 pKa = 11.84WEE318 pKa = 4.2EE319 pKa = 3.9VNAKK323 pKa = 9.48INKK326 pKa = 8.9IGNSFRR332 pKa = 11.84NVGYY336 pKa = 8.3VVRR339 pKa = 11.84GIGMGALISNISAVIPVAGSAVSAIAGIGGAATAAAGGAIGLGGAYY385 pKa = 9.41GVALGGIMAFSGQATTALQMLEE407 pKa = 4.2DD408 pKa = 4.12GQIKK412 pKa = 8.21VTSQVRR418 pKa = 11.84RR419 pKa = 11.84YY420 pKa = 7.18QTALSGLQNQWKK432 pKa = 10.05GLVQSNQAAIFNTMANGINIARR454 pKa = 11.84TALTRR459 pKa = 11.84LTPFITTTTNQIARR473 pKa = 11.84ASKK476 pKa = 10.3EE477 pKa = 3.35MRR479 pKa = 11.84NWVTSSKK486 pKa = 10.4NANNAFKK493 pKa = 10.63LINNIGPPIFQNLLNAAMRR512 pKa = 11.84VGDD515 pKa = 5.14GITHH519 pKa = 7.18MFTQFGPLFTWTGKK533 pKa = 10.72GIEE536 pKa = 4.1SLANKK541 pKa = 9.03FNAWANSASTDD552 pKa = 3.09KK553 pKa = 11.45GIAQFIQYY561 pKa = 7.64TKK563 pKa = 10.15TNLPIVGQIFGNVFSGIVSLFQAFSGHH590 pKa = 4.85SHH592 pKa = 6.93DD593 pKa = 4.88VLVGMQGVTKK603 pKa = 9.86TFKK606 pKa = 10.63DD607 pKa = 3.21WAANLQGTEE616 pKa = 3.93GFKK619 pKa = 11.27NFIAYY624 pKa = 10.39LNANGPKK631 pKa = 9.53VWQLLKK637 pKa = 10.93NIGNIFVGLVRR648 pKa = 11.84GMAPVGSVVLSITTAITGFIAKK670 pKa = 9.76GATANNTMGLMTGVLTAVGGALAAILPMWGVYY702 pKa = 7.32KK703 pKa = 10.18TVMGGASLVTRR714 pKa = 11.84AYY716 pKa = 9.23NTIVNITKK724 pKa = 9.73TSMAIWTGVTRR735 pKa = 11.84ALALAQILNARR746 pKa = 11.84NTSLATIMTGKK757 pKa = 10.63YY758 pKa = 10.42SIATKK763 pKa = 10.01IAAVATRR770 pKa = 11.84GLGLALRR777 pKa = 11.84FMTGPIGWVITAIGALVAGIMYY799 pKa = 9.88LWKK802 pKa = 10.88NNEE805 pKa = 3.78TFRR808 pKa = 11.84NFVITAWNQIKK819 pKa = 8.71ATAISVFGFLKK830 pKa = 10.38PYY832 pKa = 10.55LVAIWNGIKK841 pKa = 9.75TSAIVAWTILKK852 pKa = 8.65TAAFATWNAIKK863 pKa = 10.01FAIQNPILALRR874 pKa = 11.84NVLSAIWAGIKK885 pKa = 8.71FTAIAVWTGIKK896 pKa = 9.09TAVLTIIRR904 pKa = 11.84TWLAVAKK911 pKa = 10.37AYY913 pKa = 10.86FNVWKK918 pKa = 10.62VALTAIWNGIKK929 pKa = 9.21TVAIAVWNGIKK940 pKa = 10.53NSILSIIRR948 pKa = 11.84TLVAVARR955 pKa = 11.84AILNTIKK962 pKa = 9.96TVVSTVFNGAKK973 pKa = 9.4TIAIAAWTILKK984 pKa = 9.95NRR986 pKa = 11.84VVAITRR992 pKa = 11.84AIWTLVKK999 pKa = 10.71AIFTGLKK1006 pKa = 9.19NSVVAIFNAVKK1017 pKa = 10.62NFAIRR1022 pKa = 11.84VWTLMKK1028 pKa = 10.71NRR1030 pKa = 11.84IIANARR1036 pKa = 11.84LLWTGVRR1043 pKa = 11.84NTFNALKK1050 pKa = 9.83KK1051 pKa = 10.62GVIAIFNAVKK1061 pKa = 10.68NFAIKK1066 pKa = 10.4VWTTLKK1072 pKa = 10.73NGVIARR1078 pKa = 11.84AKK1080 pKa = 10.19SLWNGVRR1087 pKa = 11.84NTFNALRR1094 pKa = 11.84KK1095 pKa = 9.79GVTNIFNAVKK1105 pKa = 10.65NFALKK1110 pKa = 10.58LWTNMKK1116 pKa = 10.14NGVVARR1122 pKa = 11.84AKK1124 pKa = 10.6SLWNGVKK1131 pKa = 10.7NMFNKK1136 pKa = 10.31LKK1138 pKa = 10.8NSVNSITKK1146 pKa = 10.09SIRR1149 pKa = 11.84SFLFDD1154 pKa = 2.88IWNKK1158 pKa = 9.89IKK1160 pKa = 11.16NKK1162 pKa = 10.03VISLAKK1168 pKa = 10.37ALWNSVRR1175 pKa = 11.84NTFNNMRR1182 pKa = 11.84NGIRR1186 pKa = 11.84GIIGKK1191 pKa = 9.72VKK1193 pKa = 10.63GNLINTWNSIKK1204 pKa = 11.02SKK1206 pKa = 9.27VTDD1209 pKa = 3.3IASKK1213 pKa = 10.26LWNSVKK1219 pKa = 9.94GTFNNMKK1226 pKa = 10.31NGLKK1230 pKa = 10.46DD1231 pKa = 3.43IIGKK1235 pKa = 9.57IKK1237 pKa = 10.32GHH1239 pKa = 5.28ITGMVDD1245 pKa = 3.58AVKK1248 pKa = 10.61KK1249 pKa = 10.58GLNKK1253 pKa = 10.67LIDD1256 pKa = 3.78GVNWVGGKK1264 pKa = 10.29LGMDD1268 pKa = 4.17KK1269 pKa = 10.89LPKK1272 pKa = 10.23LKK1274 pKa = 10.62LHH1276 pKa = 6.7TGTEE1280 pKa = 4.35HH1281 pKa = 7.04TNTTTNVVKK1290 pKa = 10.55NGKK1293 pKa = 8.63IARR1296 pKa = 11.84DD1297 pKa = 3.55TFATVGDD1304 pKa = 3.94KK1305 pKa = 11.26GRR1307 pKa = 11.84GNGPGGFRR1315 pKa = 11.84HH1316 pKa = 6.09EE1317 pKa = 4.64AIKK1320 pKa = 10.88YY1321 pKa = 8.41PNGKK1325 pKa = 8.3MALTPNKK1332 pKa = 9.0DD1333 pKa = 2.99TTAFLPKK1340 pKa = 10.08GSSVMNGAQTHH1351 pKa = 5.7SMLSNSNPTFSKK1363 pKa = 8.87GTLPRR1368 pKa = 11.84FHH1370 pKa = 7.17NGTTGWDD1377 pKa = 3.39MLGGGGSKK1385 pKa = 10.07PKK1387 pKa = 10.16KK1388 pKa = 9.55HH1389 pKa = 6.76KK1390 pKa = 10.6KK1391 pKa = 9.81DD1392 pKa = 3.77DD1393 pKa = 3.76NLFGDD1398 pKa = 4.89MISGAKK1404 pKa = 10.17DD1405 pKa = 2.97GAEE1408 pKa = 3.8ALQGKK1413 pKa = 7.3VVKK1416 pKa = 10.42GGKK1419 pKa = 9.66AVVNKK1424 pKa = 9.23TLEE1427 pKa = 3.96TAAKK1431 pKa = 8.46GKK1433 pKa = 10.0KK1434 pKa = 8.6WLGDD1438 pKa = 3.49KK1439 pKa = 11.06VGDD1442 pKa = 3.65VMDD1445 pKa = 5.17WIEE1448 pKa = 5.16KK1449 pKa = 9.52PGKK1452 pKa = 9.47LLKK1455 pKa = 10.35KK1456 pKa = 9.07VLEE1459 pKa = 4.45GFGVSMKK1466 pKa = 10.87GFGIPKK1472 pKa = 9.7AASLPYY1478 pKa = 11.43DD1479 pKa = 3.29MMKK1482 pKa = 10.92GMFGKK1487 pKa = 10.27LKK1489 pKa = 10.45KK1490 pKa = 10.25AATDD1494 pKa = 4.66LFTSWMEE1501 pKa = 3.94EE1502 pKa = 3.65QGGDD1506 pKa = 3.32GGYY1509 pKa = 9.86IDD1511 pKa = 5.07LSKK1514 pKa = 11.13GINFGFYY1521 pKa = 8.48KK1522 pKa = 10.17TAAQALAAGYY1532 pKa = 8.57PFASAHH1538 pKa = 6.24HH1539 pKa = 6.71GIDD1542 pKa = 3.48VNYY1545 pKa = 8.48KK1546 pKa = 9.61TGDD1549 pKa = 3.37KK1550 pKa = 10.59LYY1552 pKa = 9.28STVSGTATAKK1562 pKa = 10.3HH1563 pKa = 6.57DD1564 pKa = 3.5NSGFGKK1570 pKa = 10.2HH1571 pKa = 5.48MWIKK1575 pKa = 10.49SGIMDD1580 pKa = 4.14VIYY1583 pKa = 10.47GHH1585 pKa = 5.86MSKK1588 pKa = 10.44FAFTGSKK1595 pKa = 9.49KK1596 pKa = 10.43VKK1598 pKa = 8.93PGTYY1602 pKa = 9.71LGEE1605 pKa = 4.33SGNTGRR1611 pKa = 11.84SSGPHH1616 pKa = 5.25LHH1618 pKa = 7.02YY1619 pKa = 10.82EE1620 pKa = 3.84MRR1622 pKa = 11.84KK1623 pKa = 9.66NGVPFDD1629 pKa = 3.62PRR1631 pKa = 11.84NWLKK1635 pKa = 11.03KK1636 pKa = 9.83NNGGGGKK1643 pKa = 9.36SGKK1646 pKa = 9.33WNGDD1650 pKa = 2.44IKK1652 pKa = 10.85KK1653 pKa = 9.76ALKK1656 pKa = 10.14IAGLPTSGAYY1666 pKa = 9.11VKK1668 pKa = 10.36AWQKK1672 pKa = 10.55QIQTEE1677 pKa = 4.27SGGNPKK1683 pKa = 10.46ALGGTDD1689 pKa = 3.25GLADD1693 pKa = 3.74GRR1695 pKa = 11.84AKK1697 pKa = 10.88GLVQVKK1703 pKa = 9.78PGTFNAYY1710 pKa = 9.6KK1711 pKa = 10.66LPGHH1715 pKa = 5.7GNIWNGLDD1723 pKa = 3.44NLIAGMRR1730 pKa = 11.84YY1731 pKa = 9.57AKK1733 pKa = 10.34ARR1735 pKa = 11.84YY1736 pKa = 8.67GKK1738 pKa = 10.59GGMLSVIGKK1747 pKa = 7.97GHH1749 pKa = 6.78GYY1751 pKa = 8.38ATGGLIKK1758 pKa = 10.78NAGWYY1763 pKa = 9.68NLAEE1767 pKa = 4.38GGYY1770 pKa = 8.52PEE1772 pKa = 4.7WVIPTDD1778 pKa = 3.49PSRR1781 pKa = 11.84NSDD1784 pKa = 3.3AMKK1787 pKa = 10.73LLALAAQDD1795 pKa = 3.47IQGGKK1800 pKa = 8.05STTGNKK1806 pKa = 9.58RR1807 pKa = 11.84PNSMKK1812 pKa = 10.22TPKK1815 pKa = 10.41TNDD1818 pKa = 3.14DD1819 pKa = 3.51DD1820 pKa = 5.64AMLRR1824 pKa = 11.84KK1825 pKa = 9.5VVEE1828 pKa = 4.22RR1829 pKa = 11.84QDD1831 pKa = 3.56VQISQMQEE1839 pKa = 3.82TIGYY1843 pKa = 8.24LAQLVSSTQNIEE1855 pKa = 3.95QQPKK1859 pKa = 9.55GFNEE1863 pKa = 4.13GDD1865 pKa = 3.13VSQAQGKK1872 pKa = 8.42RR1873 pKa = 11.84MQMMAYY1879 pKa = 10.61NMGGAFF1885 pKa = 3.76

Molecular weight: 203.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13571

42

1885

212.0

24.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.079 ± 0.791

0.56 ± 0.119

6.624 ± 0.531

7.501 ± 0.777

4.104 ± 0.2

6.448 ± 0.669

1.967 ± 0.198

7.125 ± 0.243

9.402 ± 0.332

7.31 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.177

6.831 ± 0.406

2.631 ± 0.146

3.677 ± 0.179

3.913 ± 0.223

5.6 ± 0.191

6.219 ± 0.292

5.6 ± 0.222

1.26 ± 0.198

4.465 ± 0.418
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski