Amino acid dipepetide frequency for Staphylococcus phage IME1354_01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.39AlaAla: 3.39 ± 1.021
0.368AlaCys: 0.368 ± 0.186
3.169AlaAsp: 3.169 ± 0.482
3.39AlaGlu: 3.39 ± 0.478
2.063AlaPhe: 2.063 ± 0.422
3.095AlaGly: 3.095 ± 0.693
1.326AlaHis: 1.326 ± 0.38
5.527AlaIle: 5.527 ± 1.342
7.074AlaLys: 7.074 ± 0.874
4.422AlaLeu: 4.422 ± 1.23
1.474AlaMet: 1.474 ± 0.348
3.832AlaAsn: 3.832 ± 0.712
1.4AlaPro: 1.4 ± 0.291
2.284AlaGln: 2.284 ± 0.811
2.874AlaArg: 2.874 ± 0.515
3.979AlaSer: 3.979 ± 0.429
3.685AlaThr: 3.685 ± 0.665
3.537AlaVal: 3.537 ± 0.929
0.958AlaTrp: 0.958 ± 0.368
2.948AlaTyr: 2.948 ± 0.426
0.0AlaXaa: 0.0 ± 0.0
Cys
0.368CysAla: 0.368 ± 0.153
0.0CysCys: 0.0 ± 0.0
0.442CysAsp: 0.442 ± 0.185
0.221CysGlu: 0.221 ± 0.105
0.147CysPhe: 0.147 ± 0.116
0.368CysGly: 0.368 ± 0.167
0.221CysHis: 0.221 ± 0.121
0.663CysIle: 0.663 ± 0.297
0.368CysLys: 0.368 ± 0.158
0.442CysLeu: 0.442 ± 0.201
0.147CysMet: 0.147 ± 0.103
0.295CysAsn: 0.295 ± 0.155
0.147CysPro: 0.147 ± 0.146
0.295CysGln: 0.295 ± 0.16
0.295CysArg: 0.295 ± 0.154
0.221CysSer: 0.221 ± 0.133
0.147CysThr: 0.147 ± 0.105
0.516CysVal: 0.516 ± 0.229
0.0CysTrp: 0.0 ± 0.0
0.295CysTyr: 0.295 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
3.685AspAla: 3.685 ± 0.53
0.295AspCys: 0.295 ± 0.19
4.864AspAsp: 4.864 ± 0.937
5.085AspGlu: 5.085 ± 0.862
2.358AspPhe: 2.358 ± 0.582
5.748AspGly: 5.748 ± 0.611
1.179AspHis: 1.179 ± 0.295
5.822AspIle: 5.822 ± 0.723
6.927AspLys: 6.927 ± 0.925
4.569AspLeu: 4.569 ± 0.681
1.99AspMet: 1.99 ± 0.33
5.527AspAsn: 5.527 ± 0.794
1.4AspPro: 1.4 ± 0.31
1.621AspGln: 1.621 ± 0.282
1.769AspArg: 1.769 ± 0.41
2.284AspSer: 2.284 ± 0.49
3.611AspThr: 3.611 ± 0.677
3.611AspVal: 3.611 ± 0.557
0.737AspTrp: 0.737 ± 0.195
3.169AspTyr: 3.169 ± 0.685
0.0AspXaa: 0.0 ± 0.0
Glu
4.274GluAla: 4.274 ± 0.551
0.737GluCys: 0.737 ± 0.236
4.053GluAsp: 4.053 ± 0.868
6.632GluGlu: 6.632 ± 1.0
3.906GluPhe: 3.906 ± 0.71
3.242GluGly: 3.242 ± 0.46
1.621GluHis: 1.621 ± 0.395
6.19GluIle: 6.19 ± 1.077
5.232GluLys: 5.232 ± 0.74
6.485GluLeu: 6.485 ± 1.037
2.8GluMet: 2.8 ± 0.433
4.053GluAsn: 4.053 ± 0.787
2.137GluPro: 2.137 ± 0.55
3.242GluGln: 3.242 ± 0.63
3.39GluArg: 3.39 ± 0.758
3.464GluSer: 3.464 ± 0.491
3.906GluThr: 3.906 ± 0.66
4.79GluVal: 4.79 ± 0.8
1.326GluTrp: 1.326 ± 0.357
3.537GluTyr: 3.537 ± 0.764
0.0GluXaa: 0.0 ± 0.0
Phe
2.063PheAla: 2.063 ± 0.471
0.221PheCys: 0.221 ± 0.12
3.095PheAsp: 3.095 ± 0.466
2.874PheGlu: 2.874 ± 0.716
1.179PhePhe: 1.179 ± 0.332
2.8PheGly: 2.8 ± 0.592
0.59PheHis: 0.59 ± 0.168
3.39PheIle: 3.39 ± 0.808
4.127PheLys: 4.127 ± 0.744
2.948PheLeu: 2.948 ± 0.449
1.179PheMet: 1.179 ± 0.226
4.053PheAsn: 4.053 ± 0.594
0.884PhePro: 0.884 ± 0.239
1.474PheGln: 1.474 ± 0.366
1.253PheArg: 1.253 ± 0.304
2.653PheSer: 2.653 ± 0.365
2.948PheThr: 2.948 ± 0.436
1.842PheVal: 1.842 ± 0.468
0.147PheTrp: 0.147 ± 0.116
1.326PheTyr: 1.326 ± 0.286
0.0PheXaa: 0.0 ± 0.0
Gly
4.2GlyAla: 4.2 ± 0.832
0.368GlyCys: 0.368 ± 0.153
4.2GlyAsp: 4.2 ± 0.627
3.39GlyGlu: 3.39 ± 0.64
3.979GlyPhe: 3.979 ± 0.546
3.906GlyGly: 3.906 ± 1.499
1.179GlyHis: 1.179 ± 0.383
5.527GlyIle: 5.527 ± 1.012
5.674GlyLys: 5.674 ± 0.896
5.158GlyLeu: 5.158 ± 0.817
1.769GlyMet: 1.769 ± 0.572
3.906GlyAsn: 3.906 ± 0.606
1.474GlyPro: 1.474 ± 0.451
2.063GlyGln: 2.063 ± 0.516
2.063GlyArg: 2.063 ± 0.383
3.537GlySer: 3.537 ± 0.58
3.758GlyThr: 3.758 ± 0.516
4.274GlyVal: 4.274 ± 0.806
0.958GlyTrp: 0.958 ± 0.259
3.095GlyTyr: 3.095 ± 0.39
0.0GlyXaa: 0.0 ± 0.0
His
1.105HisAla: 1.105 ± 0.407
0.295HisCys: 0.295 ± 0.151
1.695HisAsp: 1.695 ± 0.412
0.663HisGlu: 0.663 ± 0.162
0.516HisPhe: 0.516 ± 0.203
1.179HisGly: 1.179 ± 0.251
0.884HisHis: 0.884 ± 0.317
1.769HisIle: 1.769 ± 0.375
1.548HisLys: 1.548 ± 0.389
1.032HisLeu: 1.032 ± 0.294
0.811HisMet: 0.811 ± 0.26
1.4HisAsn: 1.4 ± 0.361
0.737HisPro: 0.737 ± 0.238
0.663HisGln: 0.663 ± 0.181
0.737HisArg: 0.737 ± 0.225
1.032HisSer: 1.032 ± 0.274
1.179HisThr: 1.179 ± 0.32
0.663HisVal: 0.663 ± 0.23
0.221HisTrp: 0.221 ± 0.14
1.548HisTyr: 1.548 ± 0.388
0.0HisXaa: 0.0 ± 0.0
Ile
4.79IleAla: 4.79 ± 0.97
0.442IleCys: 0.442 ± 0.2
5.822IleAsp: 5.822 ± 0.747
5.748IleGlu: 5.748 ± 1.031
3.021IlePhe: 3.021 ± 0.444
4.053IleGly: 4.053 ± 0.665
1.326IleHis: 1.326 ± 0.319
3.39IleIle: 3.39 ± 0.585
8.99IleLys: 8.99 ± 0.871
3.832IleLeu: 3.832 ± 0.545
1.621IleMet: 1.621 ± 0.341
4.864IleAsn: 4.864 ± 0.677
2.063IlePro: 2.063 ± 0.29
1.548IleGln: 1.548 ± 0.309
3.242IleArg: 3.242 ± 0.504
4.274IleSer: 4.274 ± 0.478
5.085IleThr: 5.085 ± 0.576
3.611IleVal: 3.611 ± 0.487
0.737IleTrp: 0.737 ± 0.443
2.8IleTyr: 2.8 ± 0.631
0.0IleXaa: 0.0 ± 0.0
Lys
5.527LysAla: 5.527 ± 0.696
0.295LysCys: 0.295 ± 0.154
7.296LysAsp: 7.296 ± 0.973
8.327LysGlu: 8.327 ± 1.266
2.653LysPhe: 2.653 ± 0.484
6.706LysGly: 6.706 ± 1.006
1.916LysHis: 1.916 ± 0.349
5.011LysIle: 5.011 ± 0.771
7.59LysLys: 7.59 ± 0.853
7.001LysLeu: 7.001 ± 0.791
3.095LysMet: 3.095 ± 0.495
6.485LysAsn: 6.485 ± 1.07
2.506LysPro: 2.506 ± 0.504
4.2LysGln: 4.2 ± 0.59
3.611LysArg: 3.611 ± 0.691
5.085LysSer: 5.085 ± 0.646
6.043LysThr: 6.043 ± 0.726
5.674LysVal: 5.674 ± 0.509
1.105LysTrp: 1.105 ± 0.296
3.906LysTyr: 3.906 ± 0.672
0.0LysXaa: 0.0 ± 0.0
Leu
4.495LeuAla: 4.495 ± 0.917
0.295LeuCys: 0.295 ± 0.169
5.822LeuAsp: 5.822 ± 0.978
6.043LeuGlu: 6.043 ± 0.88
2.579LeuPhe: 2.579 ± 0.438
3.685LeuGly: 3.685 ± 0.568
1.179LeuHis: 1.179 ± 0.297
4.79LeuIle: 4.79 ± 0.689
7.296LeuLys: 7.296 ± 0.691
5.674LeuLeu: 5.674 ± 0.756
1.326LeuMet: 1.326 ± 0.257
4.716LeuAsn: 4.716 ± 0.521
2.8LeuPro: 2.8 ± 0.537
2.8LeuGln: 2.8 ± 0.568
2.874LeuArg: 2.874 ± 0.446
4.937LeuSer: 4.937 ± 0.452
4.422LeuThr: 4.422 ± 0.591
3.316LeuVal: 3.316 ± 0.611
1.105LeuTrp: 1.105 ± 0.436
2.948LeuTyr: 2.948 ± 0.662
0.0LeuXaa: 0.0 ± 0.0
Met
2.358MetAla: 2.358 ± 0.486
0.0MetCys: 0.0 ± 0.0
1.4MetAsp: 1.4 ± 0.331
2.358MetGlu: 2.358 ± 0.66
1.105MetPhe: 1.105 ± 0.24
1.326MetGly: 1.326 ± 0.355
0.074MetHis: 0.074 ± 0.081
0.811MetIle: 0.811 ± 0.238
3.464MetLys: 3.464 ± 0.422
2.211MetLeu: 2.211 ± 0.445
0.737MetMet: 0.737 ± 0.234
1.474MetAsn: 1.474 ± 0.334
0.663MetPro: 0.663 ± 0.24
1.253MetGln: 1.253 ± 0.374
1.253MetArg: 1.253 ± 0.38
1.99MetSer: 1.99 ± 0.452
1.842MetThr: 1.842 ± 0.339
0.811MetVal: 0.811 ± 0.233
0.516MetTrp: 0.516 ± 0.166
1.179MetTyr: 1.179 ± 0.284
0.0MetXaa: 0.0 ± 0.0
Asn
4.864AsnAla: 4.864 ± 1.439
0.737AsnCys: 0.737 ± 0.255
3.685AsnAsp: 3.685 ± 0.69
5.527AsnGlu: 5.527 ± 0.779
2.358AsnPhe: 2.358 ± 0.378
6.19AsnGly: 6.19 ± 0.994
1.179AsnHis: 1.179 ± 0.384
3.906AsnIle: 3.906 ± 0.594
7.074AsnLys: 7.074 ± 0.701
3.021AsnLeu: 3.021 ± 0.46
1.548AsnMet: 1.548 ± 0.451
4.274AsnAsn: 4.274 ± 0.668
1.842AsnPro: 1.842 ± 0.369
2.358AsnGln: 2.358 ± 0.443
2.727AsnArg: 2.727 ± 0.519
4.79AsnSer: 4.79 ± 0.731
4.127AsnThr: 4.127 ± 0.707
4.053AsnVal: 4.053 ± 0.503
0.737AsnTrp: 0.737 ± 0.22
3.316AsnTyr: 3.316 ± 0.649
0.0AsnXaa: 0.0 ± 0.0
Pro
0.59ProAla: 0.59 ± 0.229
0.221ProCys: 0.221 ± 0.118
1.032ProAsp: 1.032 ± 0.325
1.842ProGlu: 1.842 ± 0.388
1.548ProPhe: 1.548 ± 0.398
1.105ProGly: 1.105 ± 0.314
0.884ProHis: 0.884 ± 0.329
2.137ProIle: 2.137 ± 0.391
2.8ProLys: 2.8 ± 0.46
1.548ProLeu: 1.548 ± 0.347
1.032ProMet: 1.032 ± 0.243
2.506ProAsn: 2.506 ± 0.391
0.884ProPro: 0.884 ± 0.24
0.811ProGln: 0.811 ± 0.325
1.179ProArg: 1.179 ± 0.272
1.769ProSer: 1.769 ± 0.379
1.548ProThr: 1.548 ± 0.337
1.179ProVal: 1.179 ± 0.212
0.074ProTrp: 0.074 ± 0.068
1.621ProTyr: 1.621 ± 0.363
0.0ProXaa: 0.0 ± 0.0
Gln
2.579GlnAla: 2.579 ± 0.459
0.074GlnCys: 0.074 ± 0.073
1.474GlnAsp: 1.474 ± 0.336
2.358GlnGlu: 2.358 ± 0.522
1.916GlnPhe: 1.916 ± 0.466
2.137GlnGly: 2.137 ± 0.436
0.958GlnHis: 0.958 ± 0.268
3.021GlnIle: 3.021 ± 0.426
2.579GlnLys: 2.579 ± 0.42
3.021GlnLeu: 3.021 ± 0.507
1.179GlnMet: 1.179 ± 0.345
2.506GlnAsn: 2.506 ± 0.681
1.179GlnPro: 1.179 ± 0.287
1.842GlnGln: 1.842 ± 0.43
1.4GlnArg: 1.4 ± 0.322
2.211GlnSer: 2.211 ± 0.413
2.358GlnThr: 2.358 ± 0.381
1.695GlnVal: 1.695 ± 0.39
0.59GlnTrp: 0.59 ± 0.244
1.695GlnTyr: 1.695 ± 0.354
0.0GlnXaa: 0.0 ± 0.0
Arg
1.916ArgAla: 1.916 ± 0.586
0.0ArgCys: 0.0 ± 0.0
2.284ArgAsp: 2.284 ± 0.545
2.653ArgGlu: 2.653 ± 0.626
1.326ArgPhe: 1.326 ± 0.28
2.653ArgGly: 2.653 ± 0.369
0.884ArgHis: 0.884 ± 0.231
2.8ArgIle: 2.8 ± 0.448
3.758ArgLys: 3.758 ± 0.673
3.39ArgLeu: 3.39 ± 0.649
1.253ArgMet: 1.253 ± 0.332
3.095ArgAsn: 3.095 ± 0.635
1.179ArgPro: 1.179 ± 0.286
1.179ArgGln: 1.179 ± 0.305
1.695ArgArg: 1.695 ± 0.407
1.842ArgSer: 1.842 ± 0.356
1.916ArgThr: 1.916 ± 0.307
2.137ArgVal: 2.137 ± 0.326
0.59ArgTrp: 0.59 ± 0.183
2.506ArgTyr: 2.506 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
3.021SerAla: 3.021 ± 0.665
0.221SerCys: 0.221 ± 0.159
4.127SerAsp: 4.127 ± 0.615
4.495SerGlu: 4.495 ± 0.862
2.653SerPhe: 2.653 ± 0.393
4.053SerGly: 4.053 ± 0.733
0.737SerHis: 0.737 ± 0.262
3.537SerIle: 3.537 ± 0.53
5.527SerLys: 5.527 ± 0.632
4.274SerLeu: 4.274 ± 0.629
1.474SerMet: 1.474 ± 0.278
3.832SerAsn: 3.832 ± 0.496
0.811SerPro: 0.811 ± 0.291
2.432SerGln: 2.432 ± 0.411
2.284SerArg: 2.284 ± 0.433
2.874SerSer: 2.874 ± 0.606
3.906SerThr: 3.906 ± 0.529
3.242SerVal: 3.242 ± 0.625
0.59SerTrp: 0.59 ± 0.183
1.916SerTyr: 1.916 ± 0.433
0.0SerXaa: 0.0 ± 0.0
Thr
4.79ThrAla: 4.79 ± 1.193
0.442ThrCys: 0.442 ± 0.178
4.79ThrAsp: 4.79 ± 0.583
3.832ThrGlu: 3.832 ± 0.683
2.727ThrPhe: 2.727 ± 0.55
5.232ThrGly: 5.232 ± 0.912
1.326ThrHis: 1.326 ± 0.294
4.2ThrIle: 4.2 ± 0.416
4.274ThrLys: 4.274 ± 0.449
4.643ThrLeu: 4.643 ± 0.727
1.105ThrMet: 1.105 ± 0.269
3.537ThrAsn: 3.537 ± 0.499
1.769ThrPro: 1.769 ± 0.359
1.916ThrGln: 1.916 ± 0.395
1.621ThrArg: 1.621 ± 0.312
3.537ThrSer: 3.537 ± 0.461
4.569ThrThr: 4.569 ± 0.596
4.569ThrVal: 4.569 ± 0.584
1.032ThrTrp: 1.032 ± 0.296
2.284ThrTyr: 2.284 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
4.2ValAla: 4.2 ± 0.762
0.368ValCys: 0.368 ± 0.178
2.948ValAsp: 2.948 ± 0.455
4.569ValGlu: 4.569 ± 0.816
2.284ValPhe: 2.284 ± 0.336
3.611ValGly: 3.611 ± 0.481
0.958ValHis: 0.958 ± 0.302
4.422ValIle: 4.422 ± 0.489
4.422ValLys: 4.422 ± 0.636
4.2ValLeu: 4.2 ± 0.477
0.958ValMet: 0.958 ± 0.225
3.464ValAsn: 3.464 ± 0.408
1.326ValPro: 1.326 ± 0.352
1.842ValGln: 1.842 ± 0.318
3.021ValArg: 3.021 ± 0.441
2.948ValSer: 2.948 ± 0.454
3.832ValThr: 3.832 ± 0.373
3.758ValVal: 3.758 ± 0.52
0.663ValTrp: 0.663 ± 0.376
2.211ValTyr: 2.211 ± 0.425
0.0ValXaa: 0.0 ± 0.0
Trp
0.59TrpAla: 0.59 ± 0.22
0.074TrpCys: 0.074 ± 0.068
0.737TrpAsp: 0.737 ± 0.224
0.516TrpGlu: 0.516 ± 0.232
0.516TrpPhe: 0.516 ± 0.23
0.663TrpGly: 0.663 ± 0.193
0.295TrpHis: 0.295 ± 0.19
0.884TrpIle: 0.884 ± 0.213
1.179TrpLys: 1.179 ± 0.217
1.253TrpLeu: 1.253 ± 0.333
0.221TrpMet: 0.221 ± 0.124
1.621TrpAsn: 1.621 ± 0.785
0.0TrpPro: 0.0 ± 0.0
1.032TrpGln: 1.032 ± 0.225
0.295TrpArg: 0.295 ± 0.138
0.221TrpSer: 0.221 ± 0.13
1.179TrpThr: 1.179 ± 0.627
0.516TrpVal: 0.516 ± 0.263
0.074TrpTrp: 0.074 ± 0.069
0.737TrpTyr: 0.737 ± 0.193
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.99TyrAla: 1.99 ± 0.355
0.147TyrCys: 0.147 ± 0.116
3.316TyrAsp: 3.316 ± 0.645
4.495TyrGlu: 4.495 ± 0.888
2.211TyrPhe: 2.211 ± 0.407
2.727TyrGly: 2.727 ± 0.43
0.958TyrHis: 0.958 ± 0.251
3.464TyrIle: 3.464 ± 0.629
4.127TyrLys: 4.127 ± 0.645
3.979TyrLeu: 3.979 ± 0.553
1.032TyrMet: 1.032 ± 0.333
2.874TyrAsn: 2.874 ± 0.324
1.105TyrPro: 1.105 ± 0.29
1.916TyrGln: 1.916 ± 0.366
1.548TyrArg: 1.548 ± 0.357
2.358TyrSer: 2.358 ± 0.44
2.137TyrThr: 2.137 ± 0.501
2.211TyrVal: 2.211 ± 0.455
0.442TyrTrp: 0.442 ± 0.19
1.621TyrTyr: 1.621 ± 0.402
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (13571 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski