Corynebacterium sp. sy039
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1918 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B8NS09|A0A5B8NS09_9CORY Pyridoxal phosphate-dependent aminotransferase OS=Corynebacterium sp. sy039 OX=2599641 GN=FQV43_00685 PE=4 SV=1
MM1 pKa = 7.86 PAFQAEE7 pKa = 4.16 VGMPYY12 pKa = 10.25 WLDD15 pKa = 3.52 LTSSDD20 pKa = 3.89 SAQSVQFYY28 pKa = 10.98 SEE30 pKa = 4.14 LFGWEE35 pKa = 3.45 ISEE38 pKa = 4.17 YY39 pKa = 10.55 SPGYY43 pKa = 9.81 HH44 pKa = 5.78 VARR47 pKa = 11.84 IQGLPVAGIVQRR59 pKa = 11.84 DD60 pKa = 3.86 KK61 pKa = 11.77 NSAQPDD67 pKa = 3.17 TWITYY72 pKa = 8.39 FLSGDD77 pKa = 3.87 IDD79 pKa = 4.05 TQCQQIAQLGGRR91 pKa = 11.84 VLAPAAEE98 pKa = 4.17 VRR100 pKa = 11.84 LGRR103 pKa = 11.84 MAIVVDD109 pKa = 3.53 NAGAAFGLIEE119 pKa = 4.82 PGGEE123 pKa = 4.03 DD124 pKa = 3.17 SFIAAGEE131 pKa = 4.02 PGTPVWHH138 pKa = 6.77 EE139 pKa = 3.72 LSCVNDD145 pKa = 3.55 YY146 pKa = 11.3 AQATQFYY153 pKa = 10.03 ADD155 pKa = 3.58 LFEE158 pKa = 4.53 WLMSEE163 pKa = 4.23 QRR165 pKa = 11.84 NEE167 pKa = 3.89 YY168 pKa = 10.62 GSYY171 pKa = 7.51 TTALVDD177 pKa = 3.85 GAAFAGILDD186 pKa = 3.86 AHH188 pKa = 6.13 QLFAAHH194 pKa = 6.62 VPNFWQSYY202 pKa = 9.51 LGVADD207 pKa = 3.16 VDD209 pKa = 3.91 AAVVRR214 pKa = 11.84 VPEE217 pKa = 4.42 LGGDD221 pKa = 4.3 IIRR224 pKa = 11.84 EE225 pKa = 4.08 PWDD228 pKa = 3.32 SAFGRR233 pKa = 11.84 MAIIADD239 pKa = 3.6 ATGATLTICEE249 pKa = 4.13 VAEE252 pKa = 4.04 PVAEE256 pKa = 4.17 GRR258 pKa = 11.84 EE259 pKa = 4.15 SDD261 pKa = 3.8 PLQDD265 pKa = 3.78 IDD267 pKa = 4.29 LSNYY271 pKa = 10.0 PEE273 pKa = 4.39 FGG275 pKa = 3.28
Molecular weight: 29.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.923
IPC_protein 3.91
Toseland 3.706
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.745
Grimsley 3.617
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.202
Thurlkill 3.745
EMBOSS 3.808
Sillero 4.024
Patrickios 0.896
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A5B8NWH0|A0A5B8NWH0_9CORY Asparaginase OS=Corynebacterium sp. sy039 OX=2599641 GN=FQV43_06825 PE=3 SV=1
MM1 pKa = 7.46 TKK3 pKa = 10.27 GKK5 pKa = 8.73 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.59 HH19 pKa = 4.59 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MATRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.3 GRR42 pKa = 11.84 ARR44 pKa = 11.84 VTAA47 pKa = 4.09
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.735
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.457
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1918
0
1918
653807
33
3042
340.9
37.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.899 ± 0.058
0.9 ± 0.017
5.513 ± 0.049
6.029 ± 0.06
3.468 ± 0.04
7.558 ± 0.045
2.506 ± 0.029
6.241 ± 0.048
3.882 ± 0.045
9.629 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.167 ± 0.026
3.144 ± 0.03
4.653 ± 0.039
4.171 ± 0.042
5.393 ± 0.055
6.501 ± 0.043
5.972 ± 0.043
7.614 ± 0.051
1.264 ± 0.022
2.496 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here