Amino acid dipepetide frequency for Saprospira grandis (strain Lewin)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.066AlaAla: 9.066 ± 0.134	1.136AlaCys: 1.136 ± 0.03	4.209AlaAsp: 4.209 ± 0.074	6.33AlaGlu: 6.33 ± 0.126	3.931AlaPhe: 3.931 ± 0.061	5.784AlaGly: 5.784 ± 0.096	1.462AlaHis: 1.462 ± 0.039	4.852AlaIle: 4.852 ± 0.091	5.642AlaLys: 5.642 ± 0.091	10.033AlaLeu: 10.033 ± 0.117	1.836AlaMet: 1.836 ± 0.042	3.528AlaAsn: 3.528 ± 0.073	3.601AlaPro: 3.601 ± 0.069	5.609AlaGln: 5.609 ± 0.099	3.245AlaArg: 3.245 ± 0.061	4.923AlaSer: 4.923 ± 0.079	3.359AlaThr: 3.359 ± 0.145	4.578AlaVal: 4.578 ± 0.074	1.071AlaTrp: 1.071 ± 0.026	3.271AlaTyr: 3.271 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.712CysAla: 0.712 ± 0.038	0.187CysCys: 0.187 ± 0.013	0.428CysAsp: 0.428 ± 0.023	0.615CysGlu: 0.615 ± 0.035	0.616CysPhe: 0.616 ± 0.025	0.765CysGly: 0.765 ± 0.026	0.185CysHis: 0.185 ± 0.014	0.503CysIle: 0.503 ± 0.017	0.466CysLys: 0.466 ± 0.021	1.141CysLeu: 1.141 ± 0.035	0.201CysMet: 0.201 ± 0.013	0.389CysAsn: 0.389 ± 0.023	0.535CysPro: 0.535 ± 0.028	0.605CysGln: 0.605 ± 0.032	0.532CysArg: 0.532 ± 0.023	0.904CysSer: 0.904 ± 0.036	0.432CysThr: 0.432 ± 0.03	0.391CysVal: 0.391 ± 0.022	0.141CysTrp: 0.141 ± 0.01	0.417CysTyr: 0.417 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.513AspAla: 3.513 ± 0.07	0.569AspCys: 0.569 ± 0.029	2.071AspAsp: 2.071 ± 0.064	3.094AspGlu: 3.094 ± 0.048	3.117AspPhe: 3.117 ± 0.058	3.301AspGly: 3.301 ± 0.073	0.751AspHis: 0.751 ± 0.026	3.082AspIle: 3.082 ± 0.056	2.697AspLys: 2.697 ± 0.056	5.612AspLeu: 5.612 ± 0.077	1.136AspMet: 1.136 ± 0.03	1.939AspAsn: 1.939 ± 0.051	2.173AspPro: 2.173 ± 0.047	2.229AspGln: 2.229 ± 0.05	2.28AspArg: 2.28 ± 0.046	3.562AspSer: 3.562 ± 0.09	1.952AspThr: 1.952 ± 0.079	2.355AspVal: 2.355 ± 0.06	0.91AspTrp: 0.91 ± 0.03	2.657AspTyr: 2.657 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	6.044GluAla: 6.044 ± 0.1	0.32GluCys: 0.32 ± 0.017	3.676GluAsp: 3.676 ± 0.058	7.435GluGlu: 7.435 ± 0.151	2.396GluPhe: 2.396 ± 0.054	4.76GluGly: 4.76 ± 0.072	1.214GluHis: 1.214 ± 0.035	3.947GluIle: 3.947 ± 0.062	5.01GluLys: 5.01 ± 0.096	8.456GluLeu: 8.456 ± 0.12	1.742GluMet: 1.742 ± 0.046	2.972GluAsn: 2.972 ± 0.054	1.707GluPro: 1.707 ± 0.041	4.606GluGln: 4.606 ± 0.087	3.576GluArg: 3.576 ± 0.057	2.794GluSer: 2.794 ± 0.047	2.47GluThr: 2.47 ± 0.051	4.037GluVal: 4.037 ± 0.074	0.668GluTrp: 0.668 ± 0.025	2.083GluTyr: 2.083 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.686PheAla: 3.686 ± 0.059	0.652PheCys: 0.652 ± 0.025	2.517PheAsp: 2.517 ± 0.049	3.197PheGlu: 3.197 ± 0.052	3.09PhePhe: 3.09 ± 0.072	3.398PheGly: 3.398 ± 0.057	0.759PheHis: 0.759 ± 0.028	2.585PheIle: 2.585 ± 0.053	2.58PheLys: 2.58 ± 0.054	5.51PheLeu: 5.51 ± 0.096	1.242PheMet: 1.242 ± 0.033	1.955PheAsn: 1.955 ± 0.047	1.717PhePro: 1.717 ± 0.035	1.794PheGln: 1.794 ± 0.044	2.146PheArg: 2.146 ± 0.042	3.909PheSer: 3.909 ± 0.067	1.769PheThr: 1.769 ± 0.045	2.494PheVal: 2.494 ± 0.044	0.967PheTrp: 0.967 ± 0.033	2.027PheTyr: 2.027 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.89GlyAla: 4.89 ± 0.087	0.896GlyCys: 0.896 ± 0.054	3.01GlyAsp: 3.01 ± 0.06	4.057GlyGlu: 4.057 ± 0.058	3.017GlyPhe: 3.017 ± 0.051	4.931GlyGly: 4.931 ± 0.111	1.381GlyHis: 1.381 ± 0.04	3.616GlyIle: 3.616 ± 0.062	3.801GlyLys: 3.801 ± 0.074	7.658GlyLeu: 7.658 ± 0.115	1.393GlyMet: 1.393 ± 0.043	2.604GlyAsn: 2.604 ± 0.075	2.903GlyPro: 2.903 ± 0.054	4.984GlyGln: 4.984 ± 0.085	3.722GlyArg: 3.722 ± 0.067	4.186GlySer: 4.186 ± 0.08	2.791GlyThr: 2.791 ± 0.102	3.265GlyVal: 3.265 ± 0.064	0.923GlyTrp: 0.923 ± 0.029	2.441GlyTyr: 2.441 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	0.987HisAla: 0.987 ± 0.031	0.259HisCys: 0.259 ± 0.014	0.582HisAsp: 0.582 ± 0.022	0.851HisGlu: 0.851 ± 0.033	1.207HisPhe: 1.207 ± 0.035	0.946HisGly: 0.946 ± 0.033	0.427HisHis: 0.427 ± 0.021	1.187HisIle: 1.187 ± 0.035	0.995HisLys: 0.995 ± 0.029	2.017HisLeu: 2.017 ± 0.049	0.255HisMet: 0.255 ± 0.013	0.711HisAsn: 0.711 ± 0.024	1.121HisPro: 1.121 ± 0.034	0.849HisGln: 0.849 ± 0.031	0.881HisArg: 0.881 ± 0.03	1.069HisSer: 1.069 ± 0.03	0.598HisThr: 0.598 ± 0.02	0.59HisVal: 0.59 ± 0.023	0.35HisTrp: 0.35 ± 0.016	1.05HisTyr: 1.05 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	4.438IleAla: 4.438 ± 0.069	0.74IleCys: 0.74 ± 0.039	3.516IleAsp: 3.516 ± 0.058	4.215IleGlu: 4.215 ± 0.071	2.779IlePhe: 2.779 ± 0.053	3.366IleGly: 3.366 ± 0.06	1.028IleHis: 1.028 ± 0.027	3.17IleIle: 3.17 ± 0.065	3.549IleLys: 3.549 ± 0.072	5.763IleLeu: 5.763 ± 0.079	1.14IleMet: 1.14 ± 0.029	2.464IleAsn: 2.464 ± 0.053	2.393IlePro: 2.393 ± 0.047	2.63IleGln: 2.63 ± 0.051	2.866IleArg: 2.866 ± 0.054	3.969IleSer: 3.969 ± 0.065	2.159IleThr: 2.159 ± 0.059	2.943IleVal: 2.943 ± 0.064	0.817IleTrp: 0.817 ± 0.028	2.414IleTyr: 2.414 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	6.187LysAla: 6.187 ± 0.106	0.234LysCys: 0.234 ± 0.014	3.079LysAsp: 3.079 ± 0.061	5.635LysGlu: 5.635 ± 0.098	1.797LysPhe: 1.797 ± 0.043	4.594LysGly: 4.594 ± 0.077	0.97LysHis: 0.97 ± 0.031	3.72LysIle: 3.72 ± 0.066	5.847LysLys: 5.847 ± 0.101	5.802LysLeu: 5.802 ± 0.086	1.892LysMet: 1.892 ± 0.039	2.872LysAsn: 2.872 ± 0.06	1.745LysPro: 1.745 ± 0.041	3.022LysGln: 3.022 ± 0.066	3.246LysArg: 3.246 ± 0.063	3.309LysSer: 3.309 ± 0.068	3.276LysThr: 3.276 ± 0.059	3.495LysVal: 3.495 ± 0.063	0.727LysTrp: 0.727 ± 0.025	2.034LysTyr: 2.034 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	11.246LeuAla: 11.246 ± 0.137	0.987LeuCys: 0.987 ± 0.027	5.808LeuAsp: 5.808 ± 0.072	7.739LeuGlu: 7.739 ± 0.113	5.541LeuPhe: 5.541 ± 0.095	8.262LeuGly: 8.262 ± 0.109	1.841LeuHis: 1.841 ± 0.044	5.444LeuIle: 5.444 ± 0.077	6.783LeuLys: 6.783 ± 0.104	13.315LeuLeu: 13.315 ± 0.191	2.297LeuMet: 2.297 ± 0.048	4.345LeuAsn: 4.345 ± 0.062	5.677LeuPro: 5.677 ± 0.091	6.023LeuGln: 6.023 ± 0.098	5.877LeuArg: 5.877 ± 0.096	8.265LeuSer: 8.265 ± 0.105	3.724LeuThr: 3.724 ± 0.061	5.366LeuVal: 5.366 ± 0.07	1.423LeuTrp: 1.423 ± 0.04	3.727LeuTyr: 3.727 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.285MetAla: 2.285 ± 0.052	0.126MetCys: 0.126 ± 0.01	1.174MetAsp: 1.174 ± 0.032	1.391MetGlu: 1.391 ± 0.038	0.66MetPhe: 0.66 ± 0.025	1.807MetGly: 1.807 ± 0.041	0.381MetHis: 0.381 ± 0.018	1.074MetIle: 1.074 ± 0.032	1.604MetLys: 1.604 ± 0.037	2.215MetLeu: 2.215 ± 0.05	0.521MetMet: 0.521 ± 0.023	0.949MetAsn: 0.949 ± 0.025	1.086MetPro: 1.086 ± 0.031	1.535MetGln: 1.535 ± 0.037	1.314MetArg: 1.314 ± 0.034	1.439MetSer: 1.439 ± 0.038	0.822MetThr: 0.822 ± 0.029	1.067MetVal: 1.067 ± 0.031	0.114MetTrp: 0.114 ± 0.01	0.579MetTyr: 0.579 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.969AsnAla: 2.969 ± 0.068	0.574AsnCys: 0.574 ± 0.032	1.877AsnAsp: 1.877 ± 0.051	2.366AsnGlu: 2.366 ± 0.046	2.245AsnPhe: 2.245 ± 0.056	3.086AsnGly: 3.086 ± 0.088	0.63AsnHis: 0.63 ± 0.025	2.73AsnIle: 2.73 ± 0.052	2.416AsnLys: 2.416 ± 0.049	4.319AsnLeu: 4.319 ± 0.067	1.115AsnMet: 1.115 ± 0.03	2.175AsnAsn: 2.175 ± 0.068	2.2AsnPro: 2.2 ± 0.059	1.928AsnGln: 1.928 ± 0.043	2.103AsnArg: 2.103 ± 0.046	2.932AsnSer: 2.932 ± 0.059	1.774AsnThr: 1.774 ± 0.055	1.957AsnVal: 1.957 ± 0.048	0.93AsnTrp: 0.93 ± 0.026	2.306AsnTyr: 2.306 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	3.898ProAla: 3.898 ± 0.063	0.375ProCys: 0.375 ± 0.016	1.998ProAsp: 1.998 ± 0.044	2.883ProGlu: 2.883 ± 0.054	2.138ProPhe: 2.138 ± 0.044	1.853ProGly: 1.853 ± 0.044	0.759ProHis: 0.759 ± 0.024	2.625ProIle: 2.625 ± 0.054	3.645ProLys: 3.645 ± 0.064	4.653ProLeu: 4.653 ± 0.073	0.9ProMet: 0.9 ± 0.027	2.325ProAsn: 2.325 ± 0.052	1.67ProPro: 1.67 ± 0.051	2.512ProGln: 2.512 ± 0.053	1.367ProArg: 1.367 ± 0.035	3.053ProSer: 3.053 ± 0.058	1.847ProThr: 1.847 ± 0.051	1.884ProVal: 1.884 ± 0.047	0.475ProTrp: 0.475 ± 0.021	1.838ProTyr: 1.838 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	5.44GlnAla: 5.44 ± 0.088	0.225GlnCys: 0.225 ± 0.013	2.411GlnAsp: 2.411 ± 0.049	4.088GlnGlu: 4.088 ± 0.077	2.16GlnPhe: 2.16 ± 0.047	3.093GlnGly: 3.093 ± 0.057	0.892GlnHis: 0.892 ± 0.026	2.943GlnIle: 2.943 ± 0.047	4.0GlnLys: 4.0 ± 0.075	7.65GlnLeu: 7.65 ± 0.112	1.252GlnMet: 1.252 ± 0.038	2.326GlnAsn: 2.326 ± 0.046	2.161GlnPro: 2.161 ± 0.046	3.304GlnGln: 3.304 ± 0.07	2.45GlnArg: 2.45 ± 0.051	2.677GlnSer: 2.677 ± 0.048	2.471GlnThr: 2.471 ± 0.048	2.142GlnVal: 2.142 ± 0.05	0.604GlnTrp: 0.604 ± 0.023	1.804GlnTyr: 1.804 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	3.837ArgAla: 3.837 ± 0.067	0.379ArgCys: 0.379 ± 0.015	1.851ArgAsp: 1.851 ± 0.041	2.706ArgGlu: 2.706 ± 0.055	2.527ArgPhe: 2.527 ± 0.046	2.5ArgGly: 2.5 ± 0.051	0.762ArgHis: 0.762 ± 0.026	2.898ArgIle: 2.898 ± 0.058	3.316ArgLys: 3.316 ± 0.069	6.103ArgLeu: 6.103 ± 0.091	1.134ArgMet: 1.134 ± 0.031	1.926ArgAsn: 1.926 ± 0.04	2.652ArgPro: 2.652 ± 0.06	2.548ArgGln: 2.548 ± 0.058	2.676ArgArg: 2.676 ± 0.061	3.193ArgSer: 3.193 ± 0.066	1.525ArgThr: 1.525 ± 0.031	1.888ArgVal: 1.888 ± 0.04	0.701ArgTrp: 0.701 ± 0.025	2.321ArgTyr: 2.321 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	5.334SerAla: 5.334 ± 0.084	0.978SerCys: 0.978 ± 0.043	2.77SerAsp: 2.77 ± 0.054	3.22SerGlu: 3.22 ± 0.057	3.757SerPhe: 3.757 ± 0.059	4.15SerGly: 4.15 ± 0.091	0.925SerHis: 0.925 ± 0.025	3.901SerIle: 3.901 ± 0.064	3.325SerLys: 3.325 ± 0.058	8.159SerLeu: 8.159 ± 0.105	1.391SerMet: 1.391 ± 0.036	2.705SerAsn: 2.705 ± 0.074	3.087SerPro: 3.087 ± 0.056	2.945SerGln: 2.945 ± 0.058	2.889SerArg: 2.889 ± 0.058	5.115SerSer: 5.115 ± 0.082	2.699SerThr: 2.699 ± 0.058	3.131SerVal: 3.131 ± 0.073	1.229SerTrp: 1.229 ± 0.037	2.893SerTyr: 2.893 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	4.612ThrAla: 4.612 ± 0.125	0.333ThrCys: 0.333 ± 0.022	2.34ThrAsp: 2.34 ± 0.073	2.814ThrGlu: 2.814 ± 0.044	1.813ThrPhe: 1.813 ± 0.049	3.127ThrGly: 3.127 ± 0.085	0.669ThrHis: 0.669 ± 0.023	2.617ThrIle: 2.617 ± 0.073	2.0ThrLys: 2.0 ± 0.038	3.925ThrLeu: 3.925 ± 0.065	0.572ThrMet: 0.572 ± 0.021	1.726ThrAsn: 1.726 ± 0.049	2.034ThrPro: 2.034 ± 0.055	1.478ThrGln: 1.478 ± 0.034	1.043ThrArg: 1.043 ± 0.033	2.214ThrSer: 2.214 ± 0.055	2.311ThrThr: 2.311 ± 0.09	2.875ThrVal: 2.875 ± 0.117	0.285ThrTrp: 0.285 ± 0.017	1.721ThrTyr: 1.721 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	4.057ValAla: 4.057 ± 0.071	0.668ValCys: 0.668 ± 0.034	3.118ValAsp: 3.118 ± 0.06	3.802ValGlu: 3.802 ± 0.06	2.56ValPhe: 2.56 ± 0.048	3.207ValGly: 3.207 ± 0.066	0.875ValHis: 0.875 ± 0.027	2.626ValIle: 2.626 ± 0.055	2.763ValLys: 2.763 ± 0.061	5.405ValLeu: 5.405 ± 0.066	0.982ValMet: 0.982 ± 0.029	2.373ValAsn: 2.373 ± 0.064	1.757ValPro: 1.757 ± 0.04	2.396ValGln: 2.396 ± 0.056	2.287ValArg: 2.287 ± 0.054	3.151ValSer: 3.151 ± 0.093	2.1ValThr: 2.1 ± 0.095	3.35ValVal: 3.35 ± 0.063	0.701ValTrp: 0.701 ± 0.025	2.156ValTyr: 2.156 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	1.451TrpAla: 1.451 ± 0.044	0.09TrpCys: 0.09 ± 0.009	0.636TrpAsp: 0.636 ± 0.026	0.98TrpGlu: 0.98 ± 0.03	0.407TrpPhe: 0.407 ± 0.021	1.162TrpGly: 1.162 ± 0.037	0.227TrpHis: 0.227 ± 0.013	0.627TrpIle: 0.627 ± 0.028	0.756TrpLys: 0.756 ± 0.029	1.351TrpLeu: 1.351 ± 0.046	0.324TrpMet: 0.324 ± 0.018	0.473TrpAsn: 0.473 ± 0.019	0.722TrpPro: 0.722 ± 0.03	0.884TrpGln: 0.884 ± 0.027	0.77TrpArg: 0.77 ± 0.026	1.089TrpSer: 1.089 ± 0.042	0.703TrpThr: 0.703 ± 0.025	0.705TrpVal: 0.705 ± 0.024	0.177TrpTrp: 0.177 ± 0.014	0.355TrpTyr: 0.355 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.811TyrAla: 2.811 ± 0.054	0.435TyrCys: 0.435 ± 0.018	1.932TyrAsp: 1.932 ± 0.05	2.496TyrGlu: 2.496 ± 0.046	2.239TyrPhe: 2.239 ± 0.05	2.378TyrGly: 2.378 ± 0.05	0.793TyrHis: 0.793 ± 0.024	2.208TyrIle: 2.208 ± 0.052	2.291TyrLys: 2.291 ± 0.056	4.602TyrLeu: 4.602 ± 0.076	0.82TyrMet: 0.82 ± 0.024	1.877TyrAsn: 1.877 ± 0.044	1.756TyrPro: 1.756 ± 0.049	2.209TyrGln: 2.209 ± 0.044	2.175TyrArg: 2.175 ± 0.046	2.85TyrSer: 2.85 ± 0.066	1.725TyrThr: 1.725 ± 0.051	1.822TyrVal: 1.822 ± 0.038	0.677TyrTrp: 0.677 ± 0.026	2.088TyrTyr: 2.088 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4218 proteins (1241634 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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