Alloscardovia omnicolens F0580
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1649 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U1SDQ6|U1SDQ6_9BIFI Branched-chain amino acid transport system carrier protein OS=Alloscardovia omnicolens F0580 OX=1321816 GN=HMPREF9244_01367 PE=3 SV=1
MM1 pKa = 7.13 AQDD4 pKa = 3.55 YY5 pKa = 7.65 DD6 pKa = 3.95 TPRR9 pKa = 11.84 SKK11 pKa = 11.32 DD12 pKa = 3.23 EE13 pKa = 4.4 DD14 pKa = 3.91 EE15 pKa = 4.63 EE16 pKa = 4.9 SIEE19 pKa = 4.23 ALGTSSRR26 pKa = 11.84 QNQNNDD32 pKa = 3.18 LDD34 pKa = 4.52 DD35 pKa = 5.29 DD36 pKa = 4.51 EE37 pKa = 5.41 NALAEE42 pKa = 4.77 GYY44 pKa = 8.06 EE45 pKa = 4.3 LPGSDD50 pKa = 5.12 LSNEE54 pKa = 4.12 DD55 pKa = 3.04 SSVTVVPMQGDD66 pKa = 3.54 EE67 pKa = 4.63 FVCSEE72 pKa = 4.28 CFLVKK77 pKa = 10.28 HH78 pKa = 6.48 RR79 pKa = 11.84 SQLAYY84 pKa = 9.35 TDD86 pKa = 4.23 EE87 pKa = 5.37 DD88 pKa = 3.89 GQPVCKK94 pKa = 10.23 EE95 pKa = 3.94 CAAA98 pKa = 4.21
Molecular weight: 10.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.732
IPC_protein 3.694
Toseland 3.49
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.617
Nozaki 3.808
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.821
Patrickios 1.837
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|U1QWS2|U1QWS2_9BIFI Phospholipase/carboxylesterase OS=Alloscardovia omnicolens F0580 OX=1321816 GN=HMPREF9244_00268 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.82 MKK15 pKa = 9.64 HH16 pKa = 5.7 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 AGRR28 pKa = 11.84 AVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.06 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1649
0
1649
522203
38
3094
316.7
34.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.15 ± 0.072
0.838 ± 0.02
5.966 ± 0.057
5.911 ± 0.054
3.751 ± 0.042
7.065 ± 0.051
2.371 ± 0.03
6.105 ± 0.058
4.302 ± 0.059
8.74 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.613 ± 0.03
3.985 ± 0.04
3.944 ± 0.034
3.852 ± 0.038
5.251 ± 0.055
6.92 ± 0.057
6.032 ± 0.046
7.875 ± 0.052
1.333 ± 0.028
2.994 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here