Rhodococcus kyotonensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4877 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177YKY3|A0A177YKY3_9NOCA Glycosyl transferase family 1 OS=Rhodococcus kyotonensis OX=398843 GN=A3K89_17900 PE=4 SV=1
MM1 pKa = 7.62GGGAVKK7 pKa = 10.44KK8 pKa = 10.34LLALAVSAVSIAGLTACAQSNDD30 pKa = 2.97SDD32 pKa = 4.18TIRR35 pKa = 11.84FALDD39 pKa = 2.82WTPNTNHH46 pKa = 6.56TGLYY50 pKa = 9.41VALQEE55 pKa = 5.7GYY57 pKa = 10.07FADD60 pKa = 3.99AGLDD64 pKa = 3.87VEE66 pKa = 4.45VLPYY70 pKa = 11.26NNTSPDD76 pKa = 3.51TLVDD80 pKa = 3.35SGNAEE85 pKa = 4.27FGISTQSSTAFAKK98 pKa = 10.68AAGAQIVSVLAPLQHH113 pKa = 6.72WATGIGVRR121 pKa = 11.84ADD123 pKa = 3.87RR124 pKa = 11.84DD125 pKa = 4.34DD126 pKa = 3.9IPSPRR131 pKa = 11.84ALDD134 pKa = 3.61GKK136 pKa = 8.94TYY138 pKa = 10.98AGFGDD143 pKa = 3.79TGEE146 pKa = 4.2QATLRR151 pKa = 11.84QVVRR155 pKa = 11.84NDD157 pKa = 3.22GGAGEE162 pKa = 4.61YY163 pKa = 7.46EE164 pKa = 4.39TVVLGTSAYY173 pKa = 8.24EE174 pKa = 3.84AVYY177 pKa = 10.71SGTADD182 pKa = 3.42FTVSYY187 pKa = 8.35FAWEE191 pKa = 4.79GIEE194 pKa = 4.44AEE196 pKa = 4.44HH197 pKa = 6.86SGTPMKK203 pKa = 10.96YY204 pKa = 10.06FDD206 pKa = 3.55YY207 pKa = 10.45TDD209 pKa = 3.24YY210 pKa = 11.5GFPDD214 pKa = 4.17AYY216 pKa = 10.58AITISGNEE224 pKa = 3.54NWLADD229 pKa = 3.95NPEE232 pKa = 4.29DD233 pKa = 3.53ATKK236 pKa = 10.19FVQALQRR243 pKa = 11.84GYY245 pKa = 10.72QLAADD250 pKa = 4.46DD251 pKa = 4.26PHH253 pKa = 7.22RR254 pKa = 11.84AAQDD258 pKa = 3.61VIDD261 pKa = 4.82ANPGAFTDD269 pKa = 3.73EE270 pKa = 4.05NLVFEE275 pKa = 4.59SQQMLADD282 pKa = 4.29SYY284 pKa = 11.21MKK286 pKa = 10.48DD287 pKa = 2.92ASGTVGTQNLDD298 pKa = 2.45KK299 pKa = 10.12WAAYY303 pKa = 10.67SKK305 pKa = 10.4FLYY308 pKa = 10.78DD309 pKa = 3.47NGVLVDD315 pKa = 3.96EE316 pKa = 5.25SGAPLTSEE324 pKa = 5.03PDD326 pKa = 2.7WSTYY330 pKa = 8.29FTDD333 pKa = 6.18DD334 pKa = 3.65YY335 pKa = 11.64LADD338 pKa = 3.51QQ339 pKa = 4.39

Molecular weight: 36.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177Y9F6|A0A177Y9F6_9NOCA Uncharacterized protein OS=Rhodococcus kyotonensis OX=398843 GN=A3K89_10830 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.01GRR42 pKa = 11.84KK43 pKa = 8.43EE44 pKa = 3.47LTAA47 pKa = 4.94

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4877

0

4877

1626480

33

8883

333.5

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.5 ± 0.046

0.676 ± 0.01

6.717 ± 0.033

5.436 ± 0.031

3.112 ± 0.021

8.72 ± 0.03

2.104 ± 0.016

4.354 ± 0.024

2.319 ± 0.028

9.753 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.968 ± 0.015

2.129 ± 0.021

5.261 ± 0.025

2.728 ± 0.018

6.925 ± 0.032

6.205 ± 0.021

6.354 ± 0.023

9.301 ± 0.044

1.391 ± 0.014

2.044 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski