Amino acid dipepetide frequency for Halomonas sp. ND22Bw

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.639AlaAla: 14.639 ± 0.17
1.198AlaCys: 1.198 ± 0.032
6.835AlaAsp: 6.835 ± 0.08
8.925AlaGlu: 8.925 ± 0.123
4.185AlaPhe: 4.185 ± 0.062
10.552AlaGly: 10.552 ± 0.105
2.418AlaHis: 2.418 ± 0.049
5.285AlaIle: 5.285 ± 0.064
2.54AlaLys: 2.54 ± 0.054
15.043AlaLeu: 15.043 ± 0.157
3.612AlaMet: 3.612 ± 0.056
2.482AlaAsn: 2.482 ± 0.046
5.797AlaPro: 5.797 ± 0.079
3.805AlaGln: 3.805 ± 0.054
9.452AlaArg: 9.452 ± 0.103
6.488AlaSer: 6.488 ± 0.083
5.503AlaThr: 5.503 ± 0.061
8.121AlaVal: 8.121 ± 0.083
2.043AlaTrp: 2.043 ± 0.049
2.404AlaTyr: 2.404 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.918CysAla: 0.918 ± 0.03
0.101CysCys: 0.101 ± 0.01
0.536CysAsp: 0.536 ± 0.021
0.514CysGlu: 0.514 ± 0.021
0.299CysPhe: 0.299 ± 0.017
0.91CysGly: 0.91 ± 0.029
0.29CysHis: 0.29 ± 0.016
0.317CysIle: 0.317 ± 0.016
0.171CysLys: 0.171 ± 0.012
1.019CysLeu: 1.019 ± 0.029
0.155CysMet: 0.155 ± 0.011
0.186CysAsn: 0.186 ± 0.013
0.528CysPro: 0.528 ± 0.023
0.328CysGln: 0.328 ± 0.016
0.787CysArg: 0.787 ± 0.025
0.396CysSer: 0.396 ± 0.022
0.383CysThr: 0.383 ± 0.018
0.573CysVal: 0.573 ± 0.024
0.139CysTrp: 0.139 ± 0.01
0.225CysTyr: 0.225 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
7.808AspAla: 7.808 ± 0.092
0.492AspCys: 0.492 ± 0.018
4.502AspAsp: 4.502 ± 0.073
4.481AspGlu: 4.481 ± 0.068
2.181AspPhe: 2.181 ± 0.049
5.596AspGly: 5.596 ± 0.076
1.521AspHis: 1.521 ± 0.039
2.627AspIle: 2.627 ± 0.054
1.374AspLys: 1.374 ± 0.041
5.938AspLeu: 5.938 ± 0.076
1.309AspMet: 1.309 ± 0.035
1.369AspAsn: 1.369 ± 0.036
3.269AspPro: 3.269 ± 0.057
1.939AspGln: 1.939 ± 0.043
4.296AspArg: 4.296 ± 0.06
2.496AspSer: 2.496 ± 0.056
2.869AspThr: 2.869 ± 0.054
4.056AspVal: 4.056 ± 0.067
1.161AspTrp: 1.161 ± 0.032
1.713AspTyr: 1.713 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
10.013GluAla: 10.013 ± 0.107
0.466GluCys: 0.466 ± 0.022
3.378GluAsp: 3.378 ± 0.062
3.922GluGlu: 3.922 ± 0.079
1.586GluPhe: 1.586 ± 0.045
5.37GluGly: 5.37 ± 0.08
1.806GluHis: 1.806 ± 0.045
2.843GluIle: 2.843 ± 0.056
1.569GluLys: 1.569 ± 0.045
6.975GluLeu: 6.975 ± 0.087
1.469GluMet: 1.469 ± 0.034
1.321GluAsn: 1.321 ± 0.033
2.992GluPro: 2.992 ± 0.065
2.846GluGln: 2.846 ± 0.061
6.85GluArg: 6.85 ± 0.095
2.955GluSer: 2.955 ± 0.052
3.572GluThr: 3.572 ± 0.058
4.821GluVal: 4.821 ± 0.07
0.845GluTrp: 0.845 ± 0.026
1.113GluTyr: 1.113 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.826PheAla: 3.826 ± 0.067
0.344PheCys: 0.344 ± 0.016
2.577PheAsp: 2.577 ± 0.045
2.142PheGlu: 2.142 ± 0.042
1.213PhePhe: 1.213 ± 0.04
3.18PheGly: 3.18 ± 0.051
0.823PheHis: 0.823 ± 0.027
1.417PheIle: 1.417 ± 0.037
0.774PheLys: 0.774 ± 0.029
3.165PheLeu: 3.165 ± 0.061
0.789PheMet: 0.789 ± 0.026
0.924PheAsn: 0.924 ± 0.029
1.393PhePro: 1.393 ± 0.038
1.08PheGln: 1.08 ± 0.025
2.127PheArg: 2.127 ± 0.042
1.981PheSer: 1.981 ± 0.046
1.839PheThr: 1.839 ± 0.04
2.429PheVal: 2.429 ± 0.052
0.466PheTrp: 0.466 ± 0.024
0.881PheTyr: 0.881 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
8.545GlyAla: 8.545 ± 0.096
0.91GlyCys: 0.91 ± 0.027
5.071GlyAsp: 5.071 ± 0.075
6.391GlyGlu: 6.391 ± 0.078
3.345GlyPhe: 3.345 ± 0.054
7.459GlyGly: 7.459 ± 0.107
2.188GlyHis: 2.188 ± 0.046
4.185GlyIle: 4.185 ± 0.071
2.393GlyLys: 2.393 ± 0.052
10.086GlyLeu: 10.086 ± 0.13
2.5GlyMet: 2.5 ± 0.05
1.965GlyAsn: 1.965 ± 0.046
3.155GlyPro: 3.155 ± 0.055
3.214GlyGln: 3.214 ± 0.052
6.587GlyArg: 6.587 ± 0.091
4.182GlySer: 4.182 ± 0.064
3.828GlyThr: 3.828 ± 0.063
6.595GlyVal: 6.595 ± 0.078
1.481GlyTrp: 1.481 ± 0.039
2.37GlyTyr: 2.37 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
2.667HisAla: 2.667 ± 0.048
0.279HisCys: 0.279 ± 0.018
1.672HisAsp: 1.672 ± 0.044
1.419HisGlu: 1.419 ± 0.032
0.919HisPhe: 0.919 ± 0.027
2.303HisGly: 2.303 ± 0.047
0.758HisHis: 0.758 ± 0.026
0.792HisIle: 0.792 ± 0.027
0.481HisLys: 0.481 ± 0.02
2.672HisLeu: 2.672 ± 0.049
0.434HisMet: 0.434 ± 0.02
0.506HisAsn: 0.506 ± 0.022
1.58HisPro: 1.58 ± 0.033
0.883HisGln: 0.883 ± 0.027
2.05HisArg: 2.05 ± 0.044
0.998HisSer: 0.998 ± 0.029
0.889HisThr: 0.889 ± 0.026
1.448HisVal: 1.448 ± 0.038
0.437HisTrp: 0.437 ± 0.018
0.788HisTyr: 0.788 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.631IleAla: 5.631 ± 0.076
0.39IleCys: 0.39 ± 0.022
3.331IleAsp: 3.331 ± 0.048
3.22IleGlu: 3.22 ± 0.056
1.266IlePhe: 1.266 ± 0.031
4.215IleGly: 4.215 ± 0.064
0.93IleHis: 0.93 ± 0.027
1.631IleIle: 1.631 ± 0.045
1.087IleLys: 1.087 ± 0.03
3.57IleLeu: 3.57 ± 0.071
0.796IleMet: 0.796 ± 0.026
1.236IleAsn: 1.236 ± 0.032
1.892IlePro: 1.892 ± 0.041
1.261IleGln: 1.261 ± 0.033
2.882IleArg: 2.882 ± 0.046
2.16IleSer: 2.16 ± 0.044
2.356IleThr: 2.356 ± 0.044
3.154IleVal: 3.154 ± 0.058
0.438IleTrp: 0.438 ± 0.018
0.863IleTyr: 0.863 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.047LysAla: 3.047 ± 0.076
0.131LysCys: 0.131 ± 0.011
1.189LysAsp: 1.189 ± 0.038
1.257LysGlu: 1.257 ± 0.038
0.552LysPhe: 0.552 ± 0.023
2.038LysGly: 2.038 ± 0.043
0.504LysHis: 0.504 ± 0.02
0.954LysIle: 0.954 ± 0.033
0.808LysLys: 0.808 ± 0.031
2.399LysLeu: 2.399 ± 0.046
0.522LysMet: 0.522 ± 0.02
0.532LysAsn: 0.532 ± 0.024
1.396LysPro: 1.396 ± 0.044
0.886LysGln: 0.886 ± 0.031
2.11LysArg: 2.11 ± 0.044
1.253LysSer: 1.253 ± 0.034
1.255LysThr: 1.255 ± 0.029
1.917LysVal: 1.917 ± 0.043
0.234LysTrp: 0.234 ± 0.014
0.431LysTyr: 0.431 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
15.908LeuAla: 15.908 ± 0.156
1.055LeuCys: 1.055 ± 0.032
7.665LeuAsp: 7.665 ± 0.101
7.939LeuGlu: 7.939 ± 0.098
3.577LeuPhe: 3.577 ± 0.068
10.022LeuGly: 10.022 ± 0.128
2.356LeuHis: 2.356 ± 0.045
4.927LeuIle: 4.927 ± 0.082
2.953LeuLys: 2.953 ± 0.054
11.79LeuLeu: 11.79 ± 0.173
2.824LeuMet: 2.824 ± 0.055
2.562LeuAsn: 2.562 ± 0.047
6.261LeuPro: 6.261 ± 0.08
3.028LeuGln: 3.028 ± 0.058
7.192LeuArg: 7.192 ± 0.085
6.493LeuSer: 6.493 ± 0.074
6.022LeuThr: 6.022 ± 0.079
8.293LeuVal: 8.293 ± 0.094
1.452LeuTrp: 1.452 ± 0.04
2.248LeuTyr: 2.248 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
3.223MetAla: 3.223 ± 0.056
0.173MetCys: 0.173 ± 0.012
1.15MetAsp: 1.15 ± 0.027
1.129MetGlu: 1.129 ± 0.033
0.637MetPhe: 0.637 ± 0.024
1.882MetGly: 1.882 ± 0.035
0.489MetHis: 0.489 ± 0.02
1.147MetIle: 1.147 ± 0.033
0.751MetLys: 0.751 ± 0.023
2.634MetLeu: 2.634 ± 0.05
0.613MetMet: 0.613 ± 0.029
0.714MetAsn: 0.714 ± 0.024
1.501MetPro: 1.501 ± 0.037
0.804MetGln: 0.804 ± 0.028
1.708MetArg: 1.708 ± 0.034
1.733MetSer: 1.733 ± 0.043
1.768MetThr: 1.768 ± 0.043
1.647MetVal: 1.647 ± 0.044
0.179MetTrp: 0.179 ± 0.012
0.32MetTyr: 0.32 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.762AsnAla: 2.762 ± 0.048
0.184AsnCys: 0.184 ± 0.012
1.406AsnAsp: 1.406 ± 0.038
1.157AsnGlu: 1.157 ± 0.03
0.733AsnPhe: 0.733 ± 0.026
1.955AsnGly: 1.955 ± 0.04
0.522AsnHis: 0.522 ± 0.022
1.02AsnIle: 1.02 ± 0.033
0.504AsnLys: 0.504 ± 0.02
2.479AsnLeu: 2.479 ± 0.039
0.51AsnMet: 0.51 ± 0.02
0.552AsnAsn: 0.552 ± 0.023
1.5AsnPro: 1.5 ± 0.036
0.754AsnGln: 0.754 ± 0.023
1.717AsnArg: 1.717 ± 0.035
0.88AsnSer: 0.88 ± 0.027
1.061AsnThr: 1.061 ± 0.033
1.709AsnVal: 1.709 ± 0.039
0.315AsnTrp: 0.315 ± 0.018
0.545AsnTyr: 0.545 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
6.217ProAla: 6.217 ± 0.095
0.329ProCys: 0.329 ± 0.018
3.534ProAsp: 3.534 ± 0.068
4.351ProGlu: 4.351 ± 0.072
1.688ProPhe: 1.688 ± 0.043
4.7ProGly: 4.7 ± 0.067
1.155ProHis: 1.155 ± 0.032
1.774ProIle: 1.774 ± 0.038
1.091ProLys: 1.091 ± 0.045
5.773ProLeu: 5.773 ± 0.078
1.22ProMet: 1.22 ± 0.034
1.052ProAsn: 1.052 ± 0.03
2.393ProPro: 2.393 ± 0.051
1.567ProGln: 1.567 ± 0.04
3.404ProArg: 3.404 ± 0.058
2.567ProSer: 2.567 ± 0.048
2.296ProThr: 2.296 ± 0.049
3.883ProVal: 3.883 ± 0.057
0.846ProTrp: 0.846 ± 0.03
1.062ProTyr: 1.062 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
5.278GlnAla: 5.278 ± 0.078
0.247GlnCys: 0.247 ± 0.015
1.762GlnAsp: 1.762 ± 0.05
1.813GlnGlu: 1.813 ± 0.047
0.948GlnPhe: 0.948 ± 0.023
3.101GlnGly: 3.101 ± 0.056
0.811GlnHis: 0.811 ± 0.029
1.293GlnIle: 1.293 ± 0.041
0.791GlnLys: 0.791 ± 0.028
3.577GlnLeu: 3.577 ± 0.051
0.706GlnMet: 0.706 ± 0.027
0.658GlnAsn: 0.658 ± 0.024
1.756GlnPro: 1.756 ± 0.048
1.795GlnGln: 1.795 ± 0.048
3.303GlnArg: 3.303 ± 0.057
1.615GlnSer: 1.615 ± 0.037
1.502GlnThr: 1.502 ± 0.034
2.51GlnVal: 2.51 ± 0.045
0.518GlnTrp: 0.518 ± 0.022
0.671GlnTyr: 0.671 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
7.555ArgAla: 7.555 ± 0.075
0.596ArgCys: 0.596 ± 0.025
4.737ArgAsp: 4.737 ± 0.068
5.747ArgGlu: 5.747 ± 0.083
3.063ArgPhe: 3.063 ± 0.054
5.239ArgGly: 5.239 ± 0.069
2.579ArgHis: 2.579 ± 0.048
3.319ArgIle: 3.319 ± 0.051
1.564ArgLys: 1.564 ± 0.035
11.248ArgLeu: 11.248 ± 0.138
1.696ArgMet: 1.696 ± 0.035
1.428ArgAsn: 1.428 ± 0.038
3.623ArgPro: 3.623 ± 0.061
3.335ArgGln: 3.335 ± 0.068
7.324ArgArg: 7.324 ± 0.105
3.124ArgSer: 3.124 ± 0.057
2.986ArgThr: 2.986 ± 0.05
5.498ArgVal: 5.498 ± 0.065
1.221ArgTrp: 1.221 ± 0.033
2.093ArgTyr: 2.093 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
5.513SerAla: 5.513 ± 0.074
0.382SerCys: 0.382 ± 0.02
2.727SerAsp: 2.727 ± 0.057
2.853SerGlu: 2.853 ± 0.052
1.717SerPhe: 1.717 ± 0.047
4.883SerGly: 4.883 ± 0.074
1.185SerHis: 1.185 ± 0.036
1.999SerIle: 1.999 ± 0.048
1.015SerLys: 1.015 ± 0.03
6.377SerLeu: 6.377 ± 0.075
1.274SerMet: 1.274 ± 0.032
1.117SerAsn: 1.117 ± 0.03
2.876SerPro: 2.876 ± 0.051
1.735SerGln: 1.735 ± 0.037
4.138SerArg: 4.138 ± 0.068
2.577SerSer: 2.577 ± 0.051
2.425SerThr: 2.425 ± 0.05
3.653SerVal: 3.653 ± 0.051
0.672SerTrp: 0.672 ± 0.024
1.099SerTyr: 1.099 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
5.411ThrAla: 5.411 ± 0.065
0.411ThrCys: 0.411 ± 0.021
2.536ThrAsp: 2.536 ± 0.05
2.535ThrGlu: 2.535 ± 0.043
1.723ThrPhe: 1.723 ± 0.031
4.379ThrGly: 4.379 ± 0.064
1.101ThrHis: 1.101 ± 0.034
1.814ThrIle: 1.814 ± 0.041
0.871ThrLys: 0.871 ± 0.033
7.172ThrLeu: 7.172 ± 0.078
0.964ThrMet: 0.964 ± 0.031
1.013ThrAsn: 1.013 ± 0.031
3.474ThrPro: 3.474 ± 0.057
1.526ThrGln: 1.526 ± 0.033
3.769ThrArg: 3.769 ± 0.058
2.439ThrSer: 2.439 ± 0.045
2.499ThrThr: 2.499 ± 0.047
3.556ThrVal: 3.556 ± 0.052
0.62ThrTrp: 0.62 ± 0.022
1.017ThrTyr: 1.017 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
8.734ValAla: 8.734 ± 0.086
0.663ValCys: 0.663 ± 0.026
4.488ValAsp: 4.488 ± 0.059
5.121ValGlu: 5.121 ± 0.068
2.448ValPhe: 2.448 ± 0.044
5.697ValGly: 5.697 ± 0.077
1.473ValHis: 1.473 ± 0.039
3.663ValIle: 3.663 ± 0.064
1.859ValLys: 1.859 ± 0.05
7.514ValLeu: 7.514 ± 0.086
2.008ValMet: 2.008 ± 0.044
1.774ValAsn: 1.774 ± 0.04
3.59ValPro: 3.59 ± 0.047
2.12ValGln: 2.12 ± 0.044
4.654ValArg: 4.654 ± 0.071
4.058ValSer: 4.058 ± 0.062
4.097ValThr: 4.097 ± 0.061
5.865ValVal: 5.865 ± 0.077
0.854ValTrp: 0.854 ± 0.029
1.512ValTyr: 1.512 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.236TrpAla: 1.236 ± 0.037
0.166TrpCys: 0.166 ± 0.012
0.608TrpAsp: 0.608 ± 0.022
0.665TrpGlu: 0.665 ± 0.024
0.484TrpPhe: 0.484 ± 0.02
0.949TrpGly: 0.949 ± 0.028
0.453TrpHis: 0.453 ± 0.02
0.569TrpIle: 0.569 ± 0.024
0.349TrpLys: 0.349 ± 0.019
2.409TrpLeu: 2.409 ± 0.059
0.381TrpMet: 0.381 ± 0.019
0.373TrpAsn: 0.373 ± 0.017
0.749TrpPro: 0.749 ± 0.027
0.859TrpGln: 0.859 ± 0.028
1.389TrpArg: 1.389 ± 0.04
0.74TrpSer: 0.74 ± 0.028
0.622TrpThr: 0.622 ± 0.024
0.938TrpVal: 0.938 ± 0.033
0.316TrpTrp: 0.316 ± 0.016
0.321TrpTyr: 0.321 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.394TyrAla: 2.394 ± 0.046
0.256TyrCys: 0.256 ± 0.016
1.292TyrAsp: 1.292 ± 0.036
1.134TyrGlu: 1.134 ± 0.031
0.81TyrPhe: 0.81 ± 0.028
2.02TyrGly: 2.02 ± 0.04
0.614TyrHis: 0.614 ± 0.019
0.733TyrIle: 0.733 ± 0.026
0.471TyrLys: 0.471 ± 0.019
2.723TyrLeu: 2.723 ± 0.049
0.421TyrMet: 0.421 ± 0.021
0.501TyrAsn: 0.501 ± 0.021
1.174TyrPro: 1.174 ± 0.032
0.89TyrGln: 0.89 ± 0.028
2.235TyrArg: 2.235 ± 0.055
1.083TyrSer: 1.083 ± 0.028
1.057TyrThr: 1.057 ± 0.032
1.525TyrVal: 1.525 ± 0.035
0.34TyrTrp: 0.34 ± 0.018
0.617TyrTyr: 0.617 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4859 proteins (1204888 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski