Candidatus Fokinia solitaria
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 720 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U8BRW6|A0A2U8BRW6_9RICK Seryl-tRNA synthetase OS=Candidatus Fokinia solitaria OX=1802984 GN=Fsol_00280 PE=4 SV=1
MM1 pKa = 7.18 KK2 pKa = 10.15 HH3 pKa = 5.32 VPQYY7 pKa = 10.31 YY8 pKa = 9.19 QFILIYY14 pKa = 10.33 LDD16 pKa = 4.05 DD17 pKa = 4.13 FTVVTILFDD26 pKa = 4.01 FLLLLEE32 pKa = 4.61 SDD34 pKa = 4.1 EE35 pKa = 4.33 MDD37 pKa = 3.04
Molecular weight: 4.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 3.808
IPC_protein 3.643
Toseland 3.452
ProMoST 3.732
Dawson 3.668
Bjellqvist 3.973
Wikipedia 3.681
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.579
Nozaki 3.834
DTASelect 4.037
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.783
Patrickios 0.477
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A2U8BS13|A0A2U8BS13_9RICK Uncharacterized protein OS=Candidatus Fokinia solitaria OX=1802984 GN=Fsol_00296 PE=4 SV=1
MM1 pKa = 6.85 ATKK4 pKa = 9.5 RR5 pKa = 11.84 TYY7 pKa = 10.28 QPSTIVRR14 pKa = 11.84 KK15 pKa = 9.42 RR16 pKa = 11.84 RR17 pKa = 11.84 HH18 pKa = 4.62 GFRR21 pKa = 11.84 ARR23 pKa = 11.84 MATVGGRR30 pKa = 11.84 AVIRR34 pKa = 11.84 NRR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 9.91 GRR41 pKa = 11.84 HH42 pKa = 4.58 VLSAA46 pKa = 3.81
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.428
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.281
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.018
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
720
0
720
247709
29
7555
344.0
38.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.616 ± 0.073
1.549 ± 0.046
5.103 ± 0.085
6.603 ± 0.159
4.498 ± 0.083
5.324 ± 0.095
2.248 ± 0.049
9.016 ± 0.102
7.565 ± 0.097
9.256 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.733 ± 0.053
5.173 ± 0.063
2.643 ± 0.056
3.434 ± 0.116
4.109 ± 0.065
7.964 ± 0.095
5.181 ± 0.083
6.552 ± 0.089
0.647 ± 0.025
3.787 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here