Desulfallas gibsoniae DSM 7213
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4313 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4KRH9|R4KRH9_9FIRM Germination protease OS=Desulfallas gibsoniae DSM 7213 OX=767817 GN=gpr PE=3 SV=1
MM1 pKa = 7.84 SDD3 pKa = 3.74 LKK5 pKa = 11.38 SKK7 pKa = 10.34 VAYY10 pKa = 9.99 LQGLASGMNVEE21 pKa = 4.8 ADD23 pKa = 3.6 SKK25 pKa = 10.8 EE26 pKa = 3.84 GKK28 pKa = 10.15 LLGGIIDD35 pKa = 3.99 VLDD38 pKa = 3.98 EE39 pKa = 4.14 FAEE42 pKa = 4.38 SFSEE46 pKa = 4.45 LEE48 pKa = 3.96 EE49 pKa = 4.14 SQEE52 pKa = 3.79 QLEE55 pKa = 4.84 EE56 pKa = 4.11 YY57 pKa = 11.02 VEE59 pKa = 4.2 TLDD62 pKa = 3.62 EE63 pKa = 5.13 DD64 pKa = 5.54 LYY66 pKa = 10.81 TLEE69 pKa = 5.2 NDD71 pKa = 3.25 MHH73 pKa = 8.09 DD74 pKa = 4.18 GEE76 pKa = 5.14 DD77 pKa = 3.45 LEE79 pKa = 5.65 EE80 pKa = 4.48 YY81 pKa = 10.33 MEE83 pKa = 4.41 VDD85 pKa = 4.0 CPRR88 pKa = 11.84 CGEE91 pKa = 4.31 TVLFDD96 pKa = 3.93 PGIIEE101 pKa = 4.89 DD102 pKa = 3.74 EE103 pKa = 4.82 DD104 pKa = 4.45 IIEE107 pKa = 4.38 VTCPNCDD114 pKa = 2.5 EE115 pKa = 4.37 VVFVNDD121 pKa = 5.22 DD122 pKa = 3.64 DD123 pKa = 6.16 FEE125 pKa = 4.51 TADD128 pKa = 3.91 EE129 pKa = 4.37 PEE131 pKa = 4.31 ILHH134 pKa = 6.16 SRR136 pKa = 11.84 NSVIEE141 pKa = 3.79 EE142 pKa = 4.52 DD143 pKa = 3.38 II144 pKa = 4.4
Molecular weight: 16.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.844
IPC2_protein 3.49
IPC_protein 3.478
Toseland 3.274
ProMoST 3.63
Dawson 3.452
Bjellqvist 3.605
Wikipedia 3.363
Rodwell 3.3
Grimsley 3.185
Solomon 3.439
Lehninger 3.389
Nozaki 3.567
DTASelect 3.732
Thurlkill 3.325
EMBOSS 3.376
Sillero 3.592
Patrickios 0.947
IPC_peptide 3.439
IPC2_peptide 3.567
IPC2.peptide.svr19 3.621
Protein with the highest isoelectric point:
>tr|R4KGV0|R4KGV0_9FIRM RNA polymerase sigma factor SigA OS=Desulfallas gibsoniae DSM 7213 OX=767817 GN=sigA PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 9.68 QPKK8 pKa = 8.57 NRR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.2 KK14 pKa = 8.3 VHH16 pKa = 5.86 GFLKK20 pKa = 10.58 RR21 pKa = 11.84 MSTKK25 pKa = 10.48 AGQNVIKK32 pKa = 10.48 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.64 GRR39 pKa = 11.84 KK40 pKa = 8.84 KK41 pKa = 10.59 LSAA44 pKa = 3.95
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 11.023
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.398
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.135
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.966
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4313
0
4313
1287869
29
2740
298.6
33.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.088 ± 0.045
1.295 ± 0.02
5.213 ± 0.029
6.594 ± 0.039
3.834 ± 0.025
7.638 ± 0.036
1.895 ± 0.017
7.129 ± 0.037
5.924 ± 0.036
9.943 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.737 ± 0.019
4.275 ± 0.027
4.172 ± 0.024
3.651 ± 0.024
5.242 ± 0.038
5.303 ± 0.028
5.147 ± 0.034
7.44 ± 0.034
1.024 ± 0.015
3.455 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here