Sulfurimonas hongkongensis
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2290 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T0KZV5|T0KZV5_9PROT Methyl-accepting chemotaxis protein OS=Sulfurimonas hongkongensis OX=1172190 GN=M947_08075 PE=4 SV=1
MM1 pKa = 7.32 FVQDD5 pKa = 5.17 DD6 pKa = 4.32 GLFVQDD12 pKa = 4.22 GGLFVQDD19 pKa = 4.56 DD20 pKa = 4.46 GLFVQSDD27 pKa = 4.02 GLFVQDD33 pKa = 4.64 DD34 pKa = 4.38 ACPVIPCLTRR44 pKa = 11.84 NLVYY48 pKa = 10.86 KK49 pKa = 10.04 LIRR52 pKa = 11.84 GFNTLYY58 pKa = 11.05
Molecular weight: 6.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.757
IPC_protein 3.681
Toseland 3.452
ProMoST 3.935
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.77
Rodwell 3.516
Grimsley 3.376
Solomon 3.694
Lehninger 3.643
Nozaki 3.884
DTASelect 4.19
Thurlkill 3.579
EMBOSS 3.77
Sillero 3.821
Patrickios 1.926
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|T0JGK4|T0JGK4_9PROT Response regulatory domain-containing protein OS=Sulfurimonas hongkongensis OX=1172190 GN=M947_02355 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.38 RR3 pKa = 11.84 TYY5 pKa = 9.96 QPHH8 pKa = 4.86 STPRR12 pKa = 11.84 KK13 pKa = 6.98 RR14 pKa = 11.84 THH16 pKa = 6.14 GFRR19 pKa = 11.84 HH20 pKa = 6.28 RR21 pKa = 11.84 MATKK25 pKa = 10.15 NGRR28 pKa = 11.84 NIINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.01 GRR39 pKa = 11.84 KK40 pKa = 8.53 KK41 pKa = 10.86 LSVV44 pKa = 3.15
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2290
0
2290
709285
29
2194
309.7
34.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.812 ± 0.054
0.86 ± 0.019
5.966 ± 0.045
7.137 ± 0.054
4.882 ± 0.039
5.668 ± 0.057
1.867 ± 0.021
8.557 ± 0.044
8.643 ± 0.062
9.744 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.623 ± 0.025
4.971 ± 0.043
2.809 ± 0.029
2.886 ± 0.029
3.56 ± 0.033
7.236 ± 0.041
4.963 ± 0.043
6.228 ± 0.047
0.694 ± 0.018
3.894 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here